LOCUS       62056_20775        4540 bp DNA     circular SYN 15-DEC-2020
DEFINITION  Cloning vector pT7-NSP1(02V0002G3), complete sequence.
ACCESSION   MN689785
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Patzina-Mehling C, Falkenhagen A, Trojnar E, Gadicherla AK, Johne R.
  TITLE     Potential of avian and mammalian species A rotaviruses to reassort 
            as explored by plasmid only-based reverse genetics
  JOURNAL   Virus Res 286, 198027 (2020)
  PUBMED    32442596
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Patzina-Mehling C, Falkenhagen A, Trojnar E, Gadicherla AK, Johne R.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2019) Unit Viruses in Food, German Federal 
            Institute for Risk Assessment, Diedersdorfer Weg 1, Berlin 12277, 
            Germany
REFERENCE   3  (bases 1 to 4540)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virus Res 
            286, 198027 (2020)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-NOV-2019) Unit Viruses in Food, German Federal Institute for 
            Risk Assessment, Diedersdorfer Weg 1, Berlin 12277, Germany"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        233..251
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           250..287
     gene            288..2021
                     /gene="NSP1"
                     /label=NSP1
     CDS             288..2021
                     /codon_start=1
                     /transl_table=11
                     /gene="NSP1"
                     /product="NSP1"
                     /label=NSP1
                     /note="derived from Rotavirus A strain 02V0002G3"
                     /protein_id="QPP12454.1"
                     /translation="MASNQLRFEYSVAKFTRSCFTPTLKSTEYVNSMQWLSKERLNRHL
                     WRTFNRYMIDDLPKGNCWTCGIFHNVYSCDFCNVNHICRSCKENRIDLCPFVSHTTCRF
                     AHDLIRIENCEKLTDDVLLHNLIPLYEKYFKILSKISRSAVWKELSEKKRRGQRMHTTS
                     SVAVDFNDCFLPTNIIAFKSVDKCNVIGYEKIYMNLLFGHYEPQRNREQMISYTNINLK
                     QFRKTFDLITNMNLQLALSRGKALNSMVNMDVSSNVVEIPYVNDQSYICQLDDSKLNYA
                     LIHFDAPEKFTLCRPILSCFHPEQLLKEIFTSWMVINVRVALTKLERSHYDSIFNLTNQ
                     ILWPNQERNYTSWLYFSYYENLVHDVLDNLQDIRKFAGASVHAARHVGLHESDCISCRK
                     YNYFIDVEMARKVSKILMKTCCYHDTRCADITTIFSISHRRNHDELVWRTFFDLRATLI
                     HNANIVRNLLNQSGLIAEFDTLCEWSDSSKLKLRHFCAKDNTEKLDLLQKFWSLSSDVR
                     DKMVKKFNINTDYYKVFEILPRDSEVLISITTEYDYELVTRSNSFKWLANILEIERIDD
                     E"
     3'UTR           2022..2371
     terminator      2532..2579
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(2748..2764)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      2812..2843
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
     promoter        2844..2946
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             2947..3804
                     /label=AmpR
                     /note="beta-lactamase"
     terminator      3879..3906
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(3918..4505)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cccgtgtaaa acgacggcca gtttatctag tcagcttgat tctagctgat cgtggaccgg
       61 aaggtgagcc agtgagttga ttgcagtcca gttacgctgg agtctgaggc tcgtcctgaa
      121 tgatatgcga ccgccggagg gttgcgtttg agacgggcga cagatcgaca ctgctcgatc
      181 cgctcgcacc ttaatgaatt ccggccggag atcctctaga ggaacctgca ggtaatacga
      241 ctcactatag gcttttaaaa gctaaacagt ggtgttggtg agacgcaatg gcttcaaacc
      301 aacttagatt cgaatattca gttgctaaat tcaccagatc atgttttact ccaacactta
      361 aatcaacaga gtacgtcaac tcaatgcaat ggctatctaa agagaggttg aatcggcatc
      421 tctggcgtac attcaataga tatatgattg atgatctgcc taaaggcaat tgctggacat
      481 gtggcatttt ccataatgtg tactcgtgtg atttttgcaa cgtcaatcat atttgtagat
      541 cttgtaagga aaacagaatt gatctttgtc catttgtgag tcatactacc tgtaggttcg
      601 cgcatgattt gattagaatt gagaattgtg agaaattaac tgacgatgtt ttgttacata
      661 atctaattcc tctatacgaa aaatacttta aaatcttaag taaaatatcg agaagtgccg
      721 tgtggaaaga gttatctgag aagaaacggc gtggacagag aatgcacact acttcttccg
      781 ttgcagttga ttttaatgat tgttttctac caacgaatat aatagcattc aaatctgttg
      841 acaaatgcaa tgtaattggc tacgagaaaa tttatatgaa tttgctgttt gggcattatg
      901 aaccgcagag aaatagagag caaatgatca gttacacaaa tattaatttg aaacagttta
      961 gaaaaacttt tgacttaatt acaaatatga atttacaact tgcgctgtcc agaggtaaag
     1021 ctttaaattc catggttaat atggatgtat cctccaatgt tgtggagata ccgtatgtga
     1081 atgatcaatc atacatttgt caactagatg actcaaaatt aaattatgct ttaattcatt
     1141 ttgatgcacc agaaaaattt acattatgta gaccaatttt gtcatgtttt catccagaac
     1201 aacttctaaa agagattttt acttcatgga tggtaataaa cgtaagagtt gctttaacga
     1261 agctagaaag gtcacactat gactcaattt ttaacttgac aaatcagatt ttatggccaa
     1321 atcaggagag aaattatacg tcatggctgt acttttcata ctatgagaat ttagtacatg
     1381 atgtgttgga caatttgcag gacattagaa aatttgctgg agctagtgtg catgctgcta
     1441 gacacgtagg attacacgaa tcagactgta ttagttgtag aaagtacaat tatttcattg
     1501 acgttgaaat ggctcgtaaa gtcagtaaga tattgatgaa aacatgttgt tatcatgata
     1561 cacgctgtgc tgatattact acaattttta gcatttcaca tagaagaaat cacgacgaat
     1621 tagtgtggag aacttttttt gatttacgtg caacactcat tcataacgca aatattgtga
     1681 gaaatctgct gaatcaatca ggcttgattg ctgagtttga tacactatgt gagtggagtg
     1741 actcttcaaa gctaaaatta agacattttt gtgcaaaaga taacacagag aaacttgatt
     1801 tgttgcaaaa gttttggagt ctaagttcag atgtcagaga caaaatggtt aaaaaattta
     1861 atataaacac tgattactac aaagtttttg agattctacc gagagactca gaagtgttga
     1921 tttcgataac tactgaatac gattatgaac ttgtaaccag atctaattca tttaagtggc
     1981 tggcgaatat tctggaaatt gaaaggattg atgatgagta aattaagaga tttgattatc
     2041 tgtattctgt tactgaaaaa tacaagaatc tagagaatta aaactaaaaa tgataaaaat
     2101 agtaaacacc aaaaaataca aaaaaaacaa acaaaaaaca aaacaaaaaa acaaaaaaac
     2161 aaaaaaacaa taaaaaacat aaaaaataaa aataaaatac aagaagtgga aaaaacaatt
     2221 gtaaaaacgt aaataaataa aaggtgaacg ataaaataaa taagaaatgc gaaatattac
     2281 aataaattaa aataaaataa aataaaataa aataaagccg cgtaacatca ctgtgattct
     2341 ggagtagtgc ccagaaaggc tttatatgac cgggtcggca tggcatctcc acctcctcgc
     2401 ggtccgacct gggcatccga aggaggacgt cgtccactcg gatggctaag ggagagctca
     2461 aaaaaaagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
     2521 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
     2581 tgaaaggagg aactatatcc ggatcgcggc cgcaagctta ttaaggatcg acgagagcag
     2641 cgcgactgga tcagttctgg acgagcgagc tgtcgtccga cccgtgatct tacggcatta
     2701 tacgtatgat cggtccacga tcagctagat tatctagtca gcttgatgtc atagctgttt
     2761 cctgaggctc aatactgacc atttaaatca tacctgacct ccatagcaga aagtcaaaag
     2821 cctccgaccg gaggcttttg acttgatcgg cacgtaagag gttccaactt tcaccataat
     2881 gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg agctaaggaa
     2941 gctaaaatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     3001 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     3061 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagttta
     3121 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     3181 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     3241 gacttggttg agtactcacc agtcacagaa aagcatctca cggatggcat gacagtaaga
     3301 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctggca
     3361 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     3421 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     3481 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     3541 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggatcactt
     3601 ctgcgctcgg ccctcccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     3661 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg catcgtagtt
     3721 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     3781 ggtgcctcac tgattaagca ttggtaatga gggcccaaat gtaatcacct ggctcacctt
     3841 cgggtgggcc tttcttgagg acctaaatgt aatcacctgg ctcaccttcg ggtgggcctt
     3901 tctgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     3961 tgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     4021 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     4081 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     4141 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4201 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     4261 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     4321 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     4381 ctgctgaagc cagttacctc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     4441 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     4501 ctcaagaaga tcctttgatt ttctaccgaa gaaaggccca
//
