LOCUS       V002847                 8270 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002847
VERSION     V002847
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8270)
  AUTHORS   Bergeron E, Zivcec M, Chakrabarti AK, Nichol ST, Albarino CG,
            Spiropoulou CF.
  TITLE     Recovery of Recombinant Crimean Congo Hemorrhagic Fever Virus
            Reveals a Function for Non-structural Glycoproteins Cleavage by
            Furin
  JOURNAL   PLoS Pathog. 11 (5), E1004879 (2015)
   PUBMED   25933376
REFERENCE   2  (bases 1 to 8270)
  AUTHORS   Bergeron E, Chakrabarti AK, Nichol ST, Albarino CG, Spiropoulou CF.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2014) Viral Special Pathogens Branch, Centers for
            Disease Control and Prevention, 1600 Clifton Rd NE, Atlanta, GA
            30333, USA
REFERENCE   3  (bases 1 to 8270)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8270)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS
            Pathog."; date: "2015"; volume: "11"; issue: "5"; pages: "E1004879"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-MAR-2014) Viral Special Pathogens Branch, Centers for Disease
            Control and Prevention, 1600 Clifton Rd NE, Atlanta, GA 30333, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(29..46)
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     promoter        705..809
                     /label="AmpR promoter"
     CDS             810..1667
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1841..2429
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     regulatory      2687..2704
                     /label="T7 promoter"
                     /note="T7 promoter"
                     /regulatory_class="promoter"
     promoter        2688..2705
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           2706..2798
                     /label="viral UTR"
                     /note="viral UTR"
     CDS             2798..7849
                     /gene="GP"
                     /label="Envelopment polyprotein"
                     /note="Envelopment polyprotein from Crimean-Congo
                     hemorrhagic fever virus (strain Nigeria/IbAr10200/1970).
                     Accession#: Q8JSZ3"
     3'UTR           7853..8071
                     /label="viral UTR"
                     /note="viral UTR"
     ncRNA           8072..8165
                     /product="Hepatitis D virus ribozyme"
                     /label="other"
                     /ncRNA_class="other"
     terminator      8224..8270
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
ORIGIN
        1 gctgaaagga ggaactatat ccggatcgag atcctctagg tacaagccta attgtgtagc
       61 atctggctta ctgaagcaga ccctatcatc tctctcgtaa actgccgtca gagtcggttt
      121 ggttggacga accttctgag tttctggtaa cgccgtcccg cacccggaaa tggtcagcga
      181 accaatcagc agggtcatcg ctagccagat cctctacgcc ggacgcatcg tggccggcat
      241 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga
      301 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
      361 cgtggccggg ggactgttgg gcgccatctc cttgcaccat tccttgcggc ggcggtgctc
      421 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt
      481 cgatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
      541 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
      601 gacaagctgt gacaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
      661 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
      721 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
      781 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
      841 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
      901 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
      961 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     1021 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     1081 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     1141 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     1201 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     1261 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     1321 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     1381 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     1441 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     1501 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     1561 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     1621 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     1681 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     1741 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     1801 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     1861 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     1921 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     1981 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2041 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2101 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2161 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2221 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2281 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2341 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2401 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2461 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     2521 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     2581 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     2641 cgcgttggcc gattcattaa tgcaggggga tctcgatccc gcgaaattaa tacgactcac
     2701 tatagtctca aagaaatact tgcggcacgt cagtacgtaa gtgttaactt tgaggaagtg
     2761 gattgagcat cttaattgca gcatacctgc taacatcatg catatatcat taatgtatgc
     2821 aatcctttgc ctacagctgt gtggtctggg agagactcat ggatcacaca atgaaactag
     2881 acacaataaa acagacacca tgacaacacc cggtgataac ccgagctctg aaccgccagt
     2941 gagcacggcc ttgtctatta cacttgaccc ctccactgtc acacccacaa caccagccag
     3001 tggattagaa ggctcagggg aagtctacac atcccctccg atcaccaccg ggagcttgcc
     3061 cctgtcggag acaacaccag aactccctgt tacaaccggc acagacacct taagcgcagg
     3121 tgatgtcgat cccagcacgc agacagccgg aggcacctcc gcaccaacag tccgcacaag
     3181 tctacccaac agccctagca caccatctac accacaagac acacaccatc ctgtgagaaa
     3241 tctactttca gtcacgagtc ctgggccaga tgaaacatca acaccctcgg gaacaggcaa
     3301 agagagctca gcaaccagta gccctcatcc agtctccaac agaccaccaa cccctcctgc
     3361 aacagcccag ggacccactg aaaatgacag tcacaacgcc actgaacacc ctgagtccct
     3421 gacacagtca gcaaccccag gcctaatgac ctctccaaca cagatagtcc acccacaaag
     3481 tgccaccccc ataaccgttc aagacacaca tcccagtcca acgaacaggt ctaaaagaaa
     3541 ccttaagatg gaaataatct tgactttatc tcagggttta aaaaagtact atgggaaaat
     3601 attaaggctt ctgcaactca ccttagagga ggacactgaa ggtctactgg aatggtgtaa
     3661 gagaaatctt ggtcttgatt gtgatgacac tttctttcaa aagagaattg aagaattctt
     3721 tataactggt gagggccatt ttaatgaagt tttacaattt agaacgccag gcacgttgag
     3781 caccacagag tcaacacctg ctgggctgcc aacagctgaa ccttttaagt cctacttcgc
     3841 caaaggattc ctctcgatag attcaggtta ctactcagcc aaatgttact caggaacatc
     3901 caattcaggg cttcaattga ttaacattac ccgacattca actagaatag ttgacacacc
     3961 tgggcctaag atcactaacc taaagaccat caactgcata aacttgaagg catcgatctt
     4021 caaagaacat agagaggttg aaatcaatgt gcttctcccc caagttgcag ttaatctctc
     4081 aaactgtcac gttgtaatca aatcacatgt ctgtgactac tctttagaca ttgacggtgc
     4141 ggtgaggctt cctcacattt accatgaagg agttttcatc ccaggaactt acaaaatagt
     4201 gatagataaa aaaaataagt tgaatgacag atgcacctta tttaccgact gtgtgataaa
     4261 aggaagggag gttcgtaaag gacagtcagt tttgaggcag tacaagacgg aaatcaggat
     4321 tggcaaggca tcaaccggct ctagaagatt gctttcagaa gaacccagtg atgactgtat
     4381 atcaagaact caactattaa ggacagagac tgcagagatc cacggcgaca actatggtgg
     4441 cccgggtgac aaaataacca tctgcaatgg ctcaactatt gtagaccaaa gactgggcag
     4501 tgaactagga tgctacacca tcaatagagt gaggtcattc aagctatgcg aaaacagtgc
     4561 cacagggaag aattgtgaaa tagacagtgt cccagttaaa tgcaggcagg gttattgcct
     4621 aagaatcact caggaaggga ggggccacgt aaaattatct aggggctcag aggttgtctt
     4681 agatgcatgc gatacaagct gtgaaataat gatacctaag ggcactggtg acatcctagt
     4741 tgactgttca ggtgggcagc aacattttct aaaggacaat ttgatagatc taggatgccc
     4801 caaaattcca ttattgggca aaatggctat ttacatttgc agaatgtcaa accaccccaa
     4861 aacaaccatg gctttcctct tctggttcag ctttggctat gtaataacct gcatactttg
     4921 caaggccatt ttttacttgt taataattgt tggaacacta gggaaaaggc tcaagcagta
     4981 tagagagttg aaacctcaga cttgcaccat atgtgagaca actcctgtaa atgcaataga
     5041 tgctgagatg catgacctca attgcagtta caacatttgt ccctactgtg catctagact
     5101 aacctcagat gggcttgcta ggcatgtgat acaatgccct aagcggaagg agaaagtgga
     5161 agaaactgaa ctgtacttga acttagaaag aattccttgg gttgtaagaa agctgttgca
     5221 ggtgtcagag tcaactggtg tggcattgaa aagaagcagt tggctgattg tgctgcttgt
     5281 gctattcact gtttcattat caccagttca atcagcaccc attggtcaag ggaagacaat
     5341 tgaggcatac cgggccaggg aagggtacac aagtatatgc ctctttgtac taggaagtat
     5401 cctatttata gtttcttgcc taatgaaagg gctggttgac agtgttggca actccttctt
     5461 ccctggactg tccatttgca aaacgtgctc cataagcagc attaatggct ttgaaattga
     5521 gtcccataag tgctattgca gcttattctg ttgcccctat tgtaggcact gctctaccga
     5581 taaagaaatt cataagctgc acttgagcat ctgcaaaaaa aggaaaaaag gaagtaatgt
     5641 catgttggct gtctgcaagc tcatgtgttt cagggccacc atggaagtaa gtaacagagc
     5701 cctgtttatc cgtagcatca tcaacaccac ttttgttttg tgcatactga tactagcagt
     5761 ttgtgttgtt agcacctcag cagtggagat ggaaaaccta ccagcaggga cctgggaaag
     5821 agaagaagac ctaacaaatt tctgtcatca ggaatgccag gttacagaga ctgaatgcct
     5881 ctgcccttat gaagctctag tactcagaaa gcctttattc ctagatagta cagctaaagg
     5941 catgaaaaat ctgctaaatt caacaagttt agaaacgagt ttatcaattg aggcaccatg
     6001 gggagcaata aatgttcagt caacctacaa accaactgtg tcaactgcaa acatagcact
     6061 cagttggagc tcagtggaac acagaggcaa taagatcttg gtttcaggca gatcagaatc
     6121 aattatgaag ctggaagaaa ggacaggaat cagctgggat ctcggtgtag aagatgcctc
     6181 tgaatctaaa ctgcttacag tatctgtcat ggacttgtct cagatgtact ctcctgtctt
     6241 cgagtactta tcaggggaca gacaggtggg agagtggccc aaagcaactt gcacaggtga
     6301 ctgcccagaa agatgtggct gcacatcatc aacctgtttg cacaaagaat ggcctcactc
     6361 aagaaattgg agatgcaatc ccacttggtg ctggggtgta gggactggct gcacctgttg
     6421 tggattagat gtgaaagacc tttttacaga ttatatgttt gtcaagtgga aagttgaata
     6481 catcaagaca gaggccatag tgtgtgtaga acttactagt caggaaaggc agtgtagctt
     6541 gattgaagcg ggcacaaggt tcaatttagg tcctgtgacc atcacactgt cagaaccaag
     6601 aaacatccaa caaaaactcc ctcctgaaat aatcacactg catcctagga tcgaagaagg
     6661 tttttttgac ctgatgcatg tgcaaaaggt gttatcggca agcacagtgt gtaagttgca
     6721 gagttgcaca catggtgtgc caggagacct acaggtctac cacatcggaa atttattaaa
     6781 aggggataag gtaaatggac atctaattca taaaattgag ccacacttca acacctcctg
     6841 gatgtcctgg gatggttgtg acctagacta ctactgcaac atgggagatt ggccttcttg
     6901 cacatacaca ggggtcaccc aacacaatca tgcttcattt gtaaacttac tcaacattga
     6961 aactgattac acaaagaact tccactttca ctctaaaagg gtcactgcac acggagatac
     7021 accacaacta gatcttaagg caagaccaac ctatggtgca ggcgagatca ctgttctggt
     7081 agaagttgct gacatggagt tacatacaaa gaagattgaa atatcaggct taaaatttgc
     7141 aagcttagct tgcacaggtt gttatgcttg tagctctggc atctcatgca aagttagaat
     7201 tcatgtggat gaaccagatg aacttacagt acatgttaaa agtgatgatc cagatgtggt
     7261 tgcagctagc tcaagtctca tggcaaggaa gcttgaattt ggaacagaca gtacatttaa
     7321 agctttctcg gccatgccta aaacttctct atgtttctac attgttgaaa gagaacactg
     7381 taagagctgc agtgaagaag acacaaaaaa atgtgttaac acaaaacttg agcaaccaca
     7441 aagcattttg atcgaacaca agggaactat aatcggaaag caaaacagca cttgcacggc
     7501 taaggcaagt tgctggttag agtcagtcaa gagttttttt tatggcctaa agaacatgct
     7561 tagtggcatt tttggcaatg tctttatggg cattttcttg ttccttgccc ccttcatcct
     7621 gttaatacta ttctttatgt ttgggtggag gatcctattc tgctttaaat gttgtagaag
     7681 aaccagaggc ctgttcaagt atagacacct caaagacgat gaagaaactg gttatagaag
     7741 gattattgaa aaactaaaca ataaaaaagg aaaaaacaaa ctgcttgatg gtgaaagact
     7801 tgctgataga agaattgccg aactgttctc tacaaaaaca cacattggct agaccaactg
     7861 aatgggcctt aaaaatgatg gcattacact gaacaatgct gtcattcatg ctgacatctt
     7921 tagttgcaac cctactacat tatcatcaca atatactaca tctaatctgc tacattgtat
     7981 ccatgtacag actctataat gcttgaaact gcctttgctc tatttactct gacctaaatc
     8041 ttgactgcgt gccgccacta tatctttgag agggtcggca tggcatctcc acctcctcgc
     8101 ggtccgacct gggcatccga aggaggacgt cgtccactcg gatggctaag ggagagctcg
     8161 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
     8221 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
//
