LOCUS       V013218                 6226 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013218
VERSION     V013218
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6226)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        141..159
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    160..184
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             199..221
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             240..257
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             276..1217
                     /gene="SIRT4"
                     /label="NAD-dependent protein lipoamidase sirtuin-4,
                     mitochondrial"
                     /note="NAD-dependent protein lipoamidase sirtuin-4,
                     mitochondrial from Homo sapiens. Accession#: Q9Y6E7"
     CDS             1227..1244
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      1311..1358
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     rep_origin      1395..1850
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(1946..2758)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      2880..3468
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(3654..3796)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(3901..4089)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     protein_bind    complement(4864..4885)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(4901..5980)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(5981..6058)
                     /label="lacI promoter"
ORIGIN
        1 tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc
       61 cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg
      121 agatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa
      181 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg ggcagcagcc
      241 atcatcatca tcatcacagc agcggccgcg gatccatgaa gatgagcttt gcgttgactt
      301 tcaggtcagc aaaaggccgt tggatcgcaa accccagcca gccgtgctcg aaagcctcca
      361 ttgggttatt tgtgccagca agtcctcctc tggaccctga gaaggtcaaa gagttacagc
      421 gcttcatcac cctttccaag agactccttg tgatgactgg ggcaggaatc tccaccgaat
      481 cggggatacc agactacagg tcagaaaaag tggggcttta tgcccgcact gaccgcaggc
      541 ccatccagca tggtgatttt gtccggagtg ccccaatccg ccagcggtac tgggcgagaa
      601 acttcgtagg ctggcctcaa ttctcctccc accagcctaa ccctgcacac tgggctttga
      661 gcacctggga gaaactcgga aagctgtact ggttggtgac ccaaaatgtg gatgctttgc
      721 acaccaaggc ggggagtcgg cgcctgacag agctccacgg atgcatggac agggtcctgt
      781 gcttggattg tggggaacag actccccggg gggtgctgca agagcgtttc caagtcctga
      841 accccacctg gagtgctgag gcccatggcc tggctcctga tggtgacgtc tttctctcag
      901 aggagcaagt ccggagcttt caggtcccaa cctgcgttca atgtggaggc catctgaaac
      961 cagatgtcgt tttcttcggg gacacagtga accctgacaa ggttgatttt gtgcacaagc
     1021 gtgtaaaaga agccgactcc ctcttggtgg tgggatcatc cttgcaggta tactctggtt
     1081 acaggtttat cctcactgcc tgggagaaga agctcccgat tgcaatactg aacattgggc
     1141 ccacacggtc ggatgacttg gcgtgtctga aactgaattc tcgttgtgga gagttgctgc
     1201 ctttgataga cccatgctga ctcgagcacc accaccacca ccactgagat ccggctgcta
     1261 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
     1321 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
     1381 gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     1441 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     1501 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     1561 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     1621 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     1681 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     1741 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     1801 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt caggtggcac
     1861 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     1921 gtatccgctc atgaattaat tcttagaaaa actcatcgag catcaaatga aactgcaatt
     1981 tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag
     2041 aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga
     2101 ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg
     2161 agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagttta tgcatttctt
     2221 tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca
     2281 aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag
     2341 gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa
     2401 tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc ccggggatcg
     2461 cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag
     2521 gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc
     2581 tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga
     2641 ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat
     2701 ccatgttgga atttaatcgc ggcctagagc aagacgtttc ccgttgaata tggctcataa
     2761 caccccttgt attactgttt atgtaagcag acagttttat tgttcatgac caaaatccct
     2821 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2881 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2941 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3001 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     3061 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3121 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3181 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3241 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3301 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3361 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3421 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3481 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3541 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     3601 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     3661 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca
     3721 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga
     3781 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     3841 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     3901 gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg
     3961 gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc
     4021 cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg
     4081 tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat
     4141 gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga
     4201 acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca
     4261 gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca
     4321 tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact
     4381 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca
     4441 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca
     4501 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc
     4561 cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac
     4621 gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt
     4681 cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc
     4741 tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc
     4801 ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc
     4861 ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     4921 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     4981 tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct
     5041 tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc
     5101 gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt
     5161 cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca
     5221 ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat
     5281 tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg
     5341 ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg
     5401 ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca
     5461 gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg
     5521 tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa
     5581 tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa
     5641 gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca
     5701 cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt
     5761 gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt
     5821 gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg
     5881 ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac
     5941 cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac
     6001 tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg
     6061 ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg
     6121 aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt
     6181 cccccggcca cggggcctgc caccataccc acgccgaaac aagcgc
//
