LOCUS       V013200                 7099 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013200
VERSION     V013200
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7099)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7099
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        141..159
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    160..184
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             199..221
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             240..257
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             276..2090
                     /gene="NFE2L2"
                     /label="Nuclear factor erythroid 2-related factor 2"
                     /note="Nuclear factor erythroid 2-related factor 2 from
                     Homo sapiens. Accession#: Q16236"
     CDS             2100..2117
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      2184..2231
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     rep_origin      2268..2723
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(2819..3631)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3753..4341
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(4527..4669)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(4774..4962)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     protein_bind    complement(5737..5758)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(5774..6853)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(6854..6931)
                     /label="lacI promoter"
ORIGIN
        1 tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc
       61 cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg
      121 agatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa
      181 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg ggcagcagcc
      241 atcatcatca tcatcacagc agcggccgcg gatccatgat ggacttggag ctgccgccgc
      301 cgggactccc gtcccagcag gacatggatt tgattgacat actttggagg caagatatag
      361 atcttggagt aagtcgagaa gtatttgact tcagtcagcg acggaaagag tatgagctgg
      421 aaaaacagaa aaaacttgaa aaggaaagac aagaacaact ccaaaaggag caagagaaag
      481 cctttttcgc tcagttacaa ctagatgaag agacaggtga atttctccca attcagccag
      541 cccagcacat ccagtcagaa accagtggat ctgccaacta ctcccaggtt gcccacattc
      601 ccaaatcaga tgctttgtac tttgatgact gcatgcagct tttggcgcag acattcccgt
      661 ttgtagatga caatgaggtt tcttcggcta cgtttcagtc acttgttcct gatattcccg
      721 gtcacatcga gagcccagtc ttcattgcta ctaatcaggc tcagtcacct gaaacttctg
      781 ttgctcaggt agcccctgtt gatttagacg gtatgcaaca ggacattgag caagtttggg
      841 aggagctatt atccattcct gagttacagt gtcttaatat tgaaaatgac aagctggttg
      901 agactaccat ggttccaagt ccagaagcca aactgacaga agttgacaat tatcattttt
      961 actcatctat accctcaatg gaaaaagaag taggtaactg tagtccacat tttcttaatg
     1021 cttttgagga ttccttcagc agcatcctct ccacagaaga ccccaaccag ttgacagtga
     1081 actcattaaa ttcagatgcc acagtcaaca cagattttgg tgatgaattt tattctgctt
     1141 tcatagctga gcccagtatc agcaacagca tgccctcacc tgctacttta agccattcac
     1201 tctctgaact tctaaatggg cccattgatg tttctgatct atcactttgc aaagctttca
     1261 accaaaacca ccctgaaagc acagcagaat tcaatgattc tgactccggc atttcactaa
     1321 acacaagtcc cagtgtggca tcaccagaac actcagtgga atcttccagc tatggagaca
     1381 cactacttgg cctcagtgat tctgaagtgg aagagctaga tagtgcccct ggaagtgtca
     1441 aacagaatgg tcctaaaaca ccagtacatt cttctgggga tatggtacaa cccttgtcac
     1501 catctcaggg gcagagcact cacgtgcatg atgcccaatg tgagaacaca ccagagaaag
     1561 aattgcctgt aagtcctggt catcggaaaa ccccattcac aaaagacaaa cattcaagcc
     1621 gcttggaggc tcatctcaca agagatgaac ttagggcaaa agctctccat atcccattcc
     1681 ctgtagaaaa aatcattaac ctccctgttg ttgacttcaa cgaaatgatg tccaaagagc
     1741 agttcaatga agctcaactt gcattaattc gggatatacg taggaggggt aagaataaag
     1801 tggctgctca gaattgcaga aaaagaaaac tggaaaatat agtagaacta gagcaagatt
     1861 tagatcattt gaaagatgaa aaagaaaaat tgctcaaaga aaaaggagaa aatgacaaaa
     1921 gccttcacct actgaaaaaa caactcagca ccttatatct cgaagttttc agcatgctac
     1981 gtgatgaaga tggaaaacct tattctccta gtgaatactc cctgcagcaa acaagagatg
     2041 gcaatgtttt ccttgttccc aaaagtaaga agccagatgt taagaaaaac tagctcgagc
     2101 accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
     2161 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
     2221 ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag
     2281 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     2341 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     2401 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     2461 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     2521 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     2581 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     2641 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     2701 caaaatatta acgtttacaa tttcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     2761 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgaatt aattcttaga
     2821 aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat
     2881 atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga
     2941 tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta
     3001 atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat
     3061 ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca ggccagccat
     3121 tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct
     3181 gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca
     3241 accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt
     3301 ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag
     3361 gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc
     3421 tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact
     3481 ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat
     3541 cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctag
     3601 agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag
     3661 cagacagttt tattgttcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     3721 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3781 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3841 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     3901 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3961 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     4021 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     4081 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     4141 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     4201 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     4261 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     4321 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     4381 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     4441 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     4501 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
     4561 ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     4621 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
     4681 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     4741 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     4801 cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc
     4861 ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat
     4921 aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg
     4981 gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg
     5041 ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt
     5101 atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac
     5161 agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat
     5221 ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat
     5281 tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg
     5341 tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa
     5401 cgacaggagc acgatcatgc gcacccgtgg ggccgccatg ccggcgataa tggcctgctt
     5461 ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat
     5521 tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc
     5581 gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt
     5641 cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa
     5701 ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat
     5761 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     5821 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt
     5881 tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca
     5941 gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg
     6001 gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac
     6061 caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg
     6121 caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac
     6181 cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga
     6241 gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta
     6301 acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt
     6361 cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg
     6421 ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt
     6481 taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt
     6541 cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag
     6601 atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc
     6661 caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca
     6721 gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt
     6781 tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg
     6841 ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac
     6901 cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac
     6961 tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg
     7021 aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata
     7081 cccacgccga aacaagcgc
//
