LOCUS       V013184                 6547 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013184
VERSION     V013184
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6547)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6547
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        141..159
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    160..184
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             199..221
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             240..257
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             282..1541
                     /gene="GSK3B"
                     /label="Glycogen synthase kinase-3 beta"
                     /note="Glycogen synthase kinase-3 beta from Homo sapiens.
                     Accession#: P49841"
     CDS             1548..1565
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      1632..1679
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     rep_origin      1716..2171
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(2267..3079)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3201..3789
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(3975..4117)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(4222..4410)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     protein_bind    complement(5185..5206)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(5222..6301)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(6302..6379)
                     /label="lacI promoter"
ORIGIN
        1 tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc
       61 cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg
      121 agatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa
      181 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccatg ggcagcagcc
      241 atcatcatca tcatcacagc agcggccgcg gatccgaatt catgtcaggg cggcccagaa
      301 ccacctcctt tgcggagagc tgcaagccgg tgcagcagcc ttcagctttt ggcagcatga
      361 aagttagcag agacaaggac ggcagcaagg tgacaacagt ggtggcaact cctgggcagg
      421 gtccagacag gccacaagaa gtcagctata cagacactaa agtgattgga aatggatcat
      481 ttggtgtggt atatcaagcc aaactttgtg attcaggaga actggtcgcc atcaagaaag
      541 tattgcagga caagagattt aagaatcgag agctccagat catgagaaag ctagatcact
      601 gtaacatagt ccgattgcgt tatttcttct actccagtgg tgagaagaaa gatgaggtct
      661 atcttaatct ggtgctggac tatgttccgg aaacagtata cagagttgcc agacactata
      721 gtcgagccaa acagacgctc cctgtgattt atgtcaagtt gtatatgtat cagctgttcc
      781 gaagtttagc ctatatccat tcctttggaa tctgccatcg ggatattaaa ccgcagaacc
      841 tcttgttgga tcctgatact gctgtattaa aactctgtga ctttggaagt gcaaagcagc
      901 tggtccgagg agaacccaat gtttcgtata tctgttctcg gtactatagg gcaccagagt
      961 tgatctttgg agccactgat tatacctcta gtatagatgt atggtctgct ggctgtgtgt
     1021 tggctgagct gttactagga caaccaatat ttccagggga tagtggtgtg gatcagttgg
     1081 tagaaataat caaggtcctg ggaactccaa caagggagca aatcagagaa atgaacccaa
     1141 actacacaga atttaaattc cctcaaatta aggcacatcc ttggactaag gtcttccgac
     1201 cccgaactcc accggaggca attgcactgt gtagccgtct gctggagtat acaccaactg
     1261 cccgactaac accactggaa gcttgtgcac attcattttt tgatgaatta cgggacccaa
     1321 atgtcaaact accaaatggg cgagacacac ctgcactctt caacttcacc actcaagaac
     1381 tgtcaagtaa tccacctctg gctaccatcc ttattcctcc tcatgctcgg attcaagcag
     1441 ctgcttcaac ccccacaaat gccacagcag cgtcagatgc taatactgga gaccgtggac
     1501 agaccaataa tgctgcttct gcatcagctt ccaactccac cctcgagcac caccaccacc
     1561 accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
     1621 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
     1681 tgaaaggagg aactatatcc ggattggcga atgggacgcg ccctgtagcg gcgcattaag
     1741 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     1801 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     1861 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     1921 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     1981 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     2041 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     2101 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     2161 gtttacaatt tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     2221 tttctaaata cattcaaata tgtatccgct catgaattaa ttcttagaaa aactcatcga
     2281 gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa
     2341 gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct
     2401 ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt
     2461 caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg
     2521 gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat
     2581 caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa
     2641 atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga
     2701 acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga
     2761 atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa
     2821 aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat
     2881 ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg
     2941 gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt
     3001 tatacccata taaatcagca tccatgttgg aatttaatcg cggcctagag caagacgttt
     3061 cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta
     3121 ttgttcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     3181 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3241 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3301 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     3361 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3421 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3481 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3541 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3601 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3661 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3721 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3781 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3841 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     3901 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     3961 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     4021 cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     4081 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     4141 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     4201 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct
     4261 gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc
     4321 gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat
     4381 gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt
     4441 catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga
     4501 tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg
     4561 ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt
     4621 tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc
     4681 tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc
     4741 tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc
     4801 attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac
     4861 gatcatgcgc acccgtgggg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg
     4921 tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc
     4981 aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca
     5041 gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc
     5101 gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg
     5161 catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg cgttgcgctc
     5221 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     5281 cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga
     5341 cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca
     5401 cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac
     5461 atgagctgtc ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc
     5521 cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg
     5581 cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac
     5641 tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc
     5701 agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt
     5761 gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga
     5821 aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag
     5881 tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc
     5941 cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc
     6001 gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg
     6061 cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg
     6121 actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg
     6181 ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg
     6241 aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca
     6301 cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
     6361 tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg
     6421 aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc
     6481 aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa
     6541 caagcgc
//
