LOCUS       62056_20565       13372 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13372)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..13372
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(563..1375)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1497..2085
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(2271..2413)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(2518..2706)
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     protein_bind    complement(3481..3502)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(3518..4597)
                     /codon_start=1
                     /label=lacI
                     /note="lac repressor"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(4598..4675)
                     /label=lacI promoter
     promoter        4984..5002
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5003..5027
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5042..5064
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5083..5100
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             5110..5127
                     /codon_start=1
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
                     /translation="LVPRGS"
     CDS             5131..9234
                     /codon_start=1
                     /label=dCas9
                     /note="catalytically dead mutant of the Cas9 endonuclease
                     from the Streptococcus pyogenes Type II CRISPR/Cas system"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             9277..13197
                     /codon_start=1
                     /label=AsCpf1
                     /note="CRISPR-associated protein from Acidaminococcus sp.
                     BV3L6 (Zetsche et al., 2015)"
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIARGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     CDS             13216..13233
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      13300..13347
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat
      601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
      661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
      721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
      781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
      841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
      901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
      961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag
     1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
     1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac
     1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa
     1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta
     2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
     2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
     2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
     2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
     2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
     2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
     2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
     2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
     3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
     3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
     3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc
     3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa
     3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc
     3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac
     3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca
     3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
     3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
     3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
     3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
     3781 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
     3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
     3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
     3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
     4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
     4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
     4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga
     4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
     4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
     4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
     4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
     4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
     4981 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa
     5041 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
     5101 agcagcggcc tggtgccgcg cggcagccat atggataaga aatactcaat aggcttagct
     5161 atcggcacaa atagcgtcgg atgggcggtg atcactgatg aatataaggt tccgtctaaa
     5221 aagttcaagg ttctgggaaa tacagaccgc cacagtatca aaaaaaatct tataggggct
     5281 cttttatttg acagtggaga gacagcggaa gcgactcgtc tcaaacggac agctcgtaga
     5341 aggtatacac gtcggaagaa tcgtatttgt tatctacagg agattttttc aaatgagatg
     5401 gcgaaagtag atgatagttt ctttcatcga cttgaagagt cttttttggt ggaagaagac
     5461 aagaagcatg aacgtcatcc tatttttgga aatatagtag atgaagttgc ttatcatgag
     5521 aaatatccaa ctatctatca tctgcgaaaa aaattggtag attctactga taaagcggat
     5581 ttgcgcttaa tctatttggc cttagcgcat atgattaagt ttcgtggtca ttttttgatt
     5641 gagggagatt taaatcctga taatagtgat gtggacaaac tatttatcca gttggtacaa
     5701 acctacaatc aattatttga agaaaaccct attaacgcaa gtggagtaga tgctaaagcg
     5761 attctttctg cacgattgag taaatcaaga cgattagaaa atctcattgc tcagctcccc
     5821 ggtgagaaga aaaatggctt atttgggaat ctcattgctt tgtcattggg tttgacccct
     5881 aattttaaat caaattttga tttggcagaa gatgctaaat tacagctttc aaaagatact
     5941 tacgatgatg atttagataa tttattggcg caaattggag atcaatatgc tgatttgttt
     6001 ttggcagcta agaatttatc agatgctatt ttactttcag atatcctaag agtaaatact
     6061 gaaataacta aggctcccct atcagcttca atgattaaac gctacgatga acatcatcaa
     6121 gacttgactc ttttaaaagc tttagttcga caacaacttc cagaaaagta taaagaaatc
     6181 ttttttgatc aatcaaaaaa cggatatgca ggttatattg atgggggagc tagccaagaa
     6241 gaattttata aatttatcaa accaatttta gaaaaaatgg atggtactga ggaattattg
     6301 gtgaaactaa atcgtgaaga tttgctgcgc aagcaacgga cctttgacaa cggctctatt
     6361 ccccatcaaa ttcacttggg tgagctgcat gctattttga gaagacaaga agacttttat
     6421 ccatttttaa aagacaatcg tgagaagatt gaaaaaatct tgacttttcg aattccttat
     6481 tatgttggtc cattggcgcg tggcaatagt cgttttgcat ggatgactcg gaagtctgaa
     6541 gaaacaatta ccccatggaa ttttgaagaa gttgtcgata aaggtgcttc agctcaatca
     6601 tttattgaac gcatgacaaa ctttgataaa aatcttccaa atgaaaaagt actaccaaaa
     6661 catagtttgc tttatgagta ttttacggtt tataacgaat tgacaaaggt caaatatgtt
     6721 actgaaggaa tgcgaaaacc agcatttctt tcaggtgaac agaagaaagc cattgttgat
     6781 ttactcttca aaacaaatcg aaaagtaacc gttaagcaat taaaagaaga ttatttcaaa
     6841 aaaatagaat gttttgatag tgttgaaatt tcaggagttg aagatagatt taatgcttca
     6901 ttaggtacct accatgattt gctaaaaatt attaaagata aagatttttt ggataatgaa
     6961 gaaaatgaag atatcttaga ggatattgtt ttaacattga ccttatttga agatagggag
     7021 atgattgagg aaagacttaa aacatatgct cacctctttg atgataaggt gatgaaacag
     7081 cttaaacgtc gccgttatac tggttgggga cgtttgtctc gaaaattgat taatggtatt
     7141 agggataagc aatctggcaa aacaatatta gattttttga aatcagatgg ttttgccaat
     7201 cgcaatttta tgcagctgat ccatgatgat agtttgacat ttaaagaaga cattcaaaaa
     7261 gcacaagtgt ctggacaagg cgatagttta catgaacata ttgcaaattt agctggtagc
     7321 cctgctatta aaaaaggtat tttacagact gtaaaagttg ttgatgaatt ggtcaaagta
     7381 atggggcggc ataagccaga aaatatcgtt attgaaatgg cacgtgaaaa tcagacaact
     7441 caaaagggcc agaaaaattc gcgagagcgt atgaaacgaa tcgaagaagg tatcaaagaa
     7501 ttaggaagtc agattcttaa agagcatcct gttgaaaata ctcaattgca aaatgaaaag
     7561 ctctatctct attatctcca aaatggaaga gacatgtatg tggaccaaga attagatatt
     7621 aatcgtttaa gtgattatga tgtcgatgcc attgttccac aaagtttcct taaagacgat
     7681 tcaatagaca ataaggtctt aacgcgttct gataaaaatc gtggtaaatc ggataacgtt
     7741 ccaagtgaag aagtagtcaa aaagatgaaa aactattgga gacaacttct aaacgccaag
     7801 ttaatcactc aacgtaagtt tgataattta acgaaagctg aacgtggagg tttgagtgaa
     7861 cttgataaag ctggttttat caaacgccaa ttggttgaaa ctcgccaaat cactaagcat
     7921 gtggcacaaa ttttggatag tcgcatgaat actaaatacg atgaaaatga taaacttatt
     7981 cgagaggtta aagtgattac cttaaaatct aaattagttt ctgacttccg aaaagatttc
     8041 caattctata aagtacgtga gattaacaat taccatcatg cccatgatgc gtatctaaat
     8101 gccgtcgttg gaactgcttt gattaagaaa tatccaaaac ttgaatcgga gtttgtctat
     8161 ggtgattata aagtttatga tgttcgtaaa atgattgcta agtctgagca agaaataggc
     8221 aaagcaaccg caaaatattt cttttactct aatatcatga acttcttcaa aacagaaatt
     8281 acacttgcaa atggagagat tcgcaaacgc cctctaatcg aaactaatgg ggaaactgga
     8341 gaaattgtct gggataaagg gcgagatttt gccacagtgc gcaaagtatt gtccatgccc
     8401 caagtcaata ttgtcaagaa aacagaagta cagacaggcg gattctccaa ggagtcaatt
     8461 ttaccaaaaa gaaattcgga caagcttatt gctcgtaaaa aagactggga tccaaaaaaa
     8521 tatggtggtt ttgatagtcc aacggtagct tattcagtcc tagtggttgc taaggtggaa
     8581 aaagggaaat cgaagaagtt aaaatccgtt aaagagttac tagggatcac aattatggaa
     8641 agaagttcct ttgaaaaaaa tccgattgac tttttagaag ctaaaggata taaggaagtt
     8701 aaaaaagact taatcattaa actacctaaa tatagtcttt ttgagttaga aaacggtcgt
     8761 aaacggatgc tggctagtgc cggagaatta caaaaaggaa atgagctggc tctgccaagc
     8821 aaatatgtga attttttata tttagctagt cattatgaaa agttgaaggg tagtccagaa
     8881 gataacgaac aaaaacaatt gtttgtggag cagcataagc attatttaga tgagattatt
     8941 gagcaaatca gtgaattttc taagcgtgtt attttagcag atgccaattt agataaagtt
     9001 cttagtgcat ataacaaaca tagagacaaa ccaatacgtg aacaagcaga aaatattatt
     9061 catttattta cgttgacgaa tcttggagct cccgctgctt ttaaatattt tgatacaaca
     9121 attgatcgta aacgatatac gtctacaaaa gaagttttag atgccactct tatccatcaa
     9181 tccatcactg gtctttatga aacacgcatt gatttgagtc agctaggagg tgacggcggc
     9241 ggtggcagcg atgaagtgga tggtggcggc ggctcaatga cccagttcga aggcttcacc
     9301 aacctgtatc aggtgagcaa aaccctgcgc tttgaactga ttccgcaggg caaaaccctg
     9361 aaacacattc aggaacaggg ctttattgaa gaagacaaag cgcgtaatga ccattacaaa
     9421 gaactgaaac cgattattga tcgtatctac aaaacctatg ccgatcagtg tctgcagctg
     9481 gttcagctgg actgggaaaa cctgagtgcg gcaattgaca gctatcgcaa agaaaaaacg
     9541 gaagaaaccc gtaatgcgct gattgaagaa caggcgacct atcgtaatgc gattcatgat
     9601 tactttattg gccgcaccga taacctgacc gatgcgatca acaaacgcca tgcggaaatt
     9661 tataaaggtc tgtttaaagc cgaactgttc aacggcaaag tgctgaaaca gctggggacc
     9721 gtgaccacca ccgaacatga aaacgcgctg ctgcgtagct ttgacaaatt taccacgtac
     9781 ttcagcggct tttacgagaa ccgcaaaaac gtgtttagcg cggaagatat tagcaccgcc
     9841 attccgcacc gcattgtgca ggataatttt ccgaaattta aagaaaactg ccatattttc
     9901 acccgtttaa tcaccgcggt gccgagcctg cgcgaacact tcgaaaatgt gaaaaaagcc
     9961 attggcattt tcgtgagcac cagtattgaa gaagtgttta gctttccgtt ttacaatcag
    10021 ctgttgaccc agacccagat tgatctgtat aaccagctgc tgggcggtat tagccgcgaa
    10081 gccggcaccg aaaagattaa aggcctgaac gaagtcctga acctggccat tcagaaaaat
    10141 gatgaaacgg cgcacattat tgcgagcctg ccgcatcgtt ttattccgct gttcaaacag
    10201 attctgagcg accgcaacac actgagcttt attctggaag aatttaaaag cgatgaagaa
    10261 gtgattcaaa gcttctgcaa atataaaacc ctgctgcgca atgaaaacgt gctggaaacc
    10321 gccgaagcgc tgttcaacga actgaatagc attgatctga cccacatttt tattagccat
    10381 aaaaaactgg aaaccattag ctcggcgctg tgcgatcatt gggataccct gcgcaacgcg
    10441 ttatacgaac gccgcattag cgaactgacg ggcaaaatta ccaaatccgc gaaagaaaaa
    10501 gtgcagcgca gcctgaaaca tgaagatatt aatctgcagg aaattattag cgcggccggc
    10561 aaagaactga gcgaagcgtt taaacagaaa accagcgaaa ttctgagcca cgcgcatgcg
    10621 gcgctggacc agccgctgcc gaccaccctg aaaaaacagg aggaaaaaga aattctgaaa
    10681 agtcagctgg atagcctgct gggcctgtac catctgctgg attggtttgc agtggatgaa
    10741 agcaacgaag tggatccgga atttagcgcg cgtctgaccg gcattaaact ggaaatggaa
    10801 ccgagcctga gcttctataa taaagcacgt aattacgcga ccaaaaaacc gtatagcgtg
    10861 gaaaaattta aactgaactt ccagatgcct accctggcga gcggttggga tgtgaataaa
    10921 gaaaaaaaca acggcgcgat tctgttcgtg aaaaatggct tatattatct gggcattatg
    10981 ccgaaacaga aaggccgcta taaagcactg agcttcgaac cgaccgaaaa aaccagcgaa
    11041 ggctttgata aaatgtatta cgattatttt ccggatgcgg cgaaaatgat tccgaaatgc
    11101 tcaacccagc tgaaagcggt gaccgcccat ttccagaccc ataccactcc gatcctgctg
    11161 agcaacaact ttattgaacc actggaaatt accaaagaaa tctatgatct gaacaacccg
    11221 gaaaaagaac cgaaaaaatt ccagaccgcc tatgcaaaaa aaaccggcga tcagaaaggt
    11281 tatcgcgaag cgctgtgtaa atggattgat tttacccgcg attttctgag caaatacacc
    11341 aaaaccacct caatcgatct gagttcgctg cgtccgagca gccagtataa agatctgggt
    11401 gaatactatg cggaactgaa cccgctgctg tatcacatct cattccagcg cattgcggaa
    11461 aaagaaatca tggatgcggt agaaaccggt aaactgtacc tgttccagat ctataataaa
    11521 gattttgcca aaggccatca cggcaaaccg aacctgcata ccctgtattg gacgggcctg
    11581 tttagcccgg aaaacctggc caaaaccagc attaaactga atggccaggc ggaattgttt
    11641 tatcgcccga aaagccgcat gaaacgcatg gcccaccgcc tgggcgaaaa aatgctgaat
    11701 aaaaaactga aagatcagaa aaccccgatt ccggataccc tgtatcagga gctgtatgac
    11761 tatgtgaatc atcgcctgtc acatgatctg agcgatgaag cccgtgcgct gctgccgaac
    11821 gttattacga aagaagtgag ccatgaaatt attaaagatc gccgttttac gagcgataaa
    11881 tttttctttc atgttcctat caccctgaac taccaggccg ccaatagccc gagcaaattt
    11941 aatcagcgcg tgaacgcgta tctgaaagaa catccggaaa ccccgattat tggcatcgcc
    12001 cgtggcgaac gcaacctgat ttacattacg gtaattgata gcaccggcaa aattctggaa
    12061 cagcgctcac tgaataccat tcagcagttc gattaccaga agaaactgga taaccgcgaa
    12121 aaagaacgtg ttgccgcgcg tcaggcctgg agcgtggttg gtaccattaa agatctgaaa
    12181 caaggctacc tgagccaggt gattcacgaa attgtggatc tgatgattca ttatcaggcc
    12241 gtggtagtgc tggaaaatct gaacttcggc tttaaaagca aacgcaccgg catcgcggaa
    12301 aaagcggtat atcagcaatt tgaaaaaatg ctgatcgata aactgaactg cctggtgctg
    12361 aaagattatc cggccgaaaa agtgggcggc gtgctgaatc cctatcagct gaccgatcag
    12421 tttaccagct tcgcgaaaat gggcacccag agcggctttc tgttttatgt gccggccccg
    12481 tataccagca aaattgatcc gctgaccggc ttcgtggatc cgttcgtgtg gaaaaccatt
    12541 aaaaatcatg aaagccgtaa acacttcctg gaaggttttg attttctgca ttacgatgtg
    12601 aaaaccggcg attttatcct gcattttaaa atgaaccgta acctgagctt tcagcgtggt
    12661 ctgccgggct tcatgccggc ctgggatatc gtctttgaaa aaaacgaaac ccagtttgat
    12721 gcgaaaggca ccccgtttat cgccggcaaa cgtattgttc cggtgattga aaatcaccgc
    12781 tttaccggcc gttatcgcga tctgtacccg gcgaacgaac tgattgcgct gctggaggaa
    12841 aaaggcattg ttttccgtga tggcagcaat attctgccga aactgctgga aaacgatgat
    12901 agtcatgcga ttgataccat ggtggccctg attcgcagcg tgctgcaaat gcgcaacagc
    12961 aatgcggcca ccggcgaaga ttatattaac agcccggtgc gtgatctgaa tggcgtttgc
    13021 tttgatagcc gtttccaaaa cccggaatgg ccgatggatg cggacgcgaa tggcgcgtat
    13081 catattgcgc tgaaaggcca gctgctgctg aaccatctga aagaaagcaa agatctgaaa
    13141 ctgcagaatg gcattagtaa tcaggattgg ctggcctata ttcaggaact gcgtaattaa
    13201 gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga
    13261 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
    13321 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg at
//
