LOCUS       V014193                11359 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014193
VERSION     V014193
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11359)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11359
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(563..1375)
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1497..2085
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(2271..2413)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(2518..2706)
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     protein_bind    complement(3481..3502)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(3518..4597)
                     /label="lacI"
                     /note="lac repressor"
     promoter        complement(4598..4675)
                     /label="lacI promoter"
     promoter        4984..5002
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5003..5027
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5042..5064
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5083..5100
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             7882..11193
                     /gene="ABL1"
                     /label="Tyrosine-protein kinase ABL1"
                     /note="Tyrosine-protein kinase ABL1 from Homo sapiens.
                     Accession#: P00519"
     CDS             11203..11220
                     /label="6xHis"
                     /note="6xHis affinity tag"
     terminator      11287..11334
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat
      601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
      661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
      721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
      781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc
      841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
      901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
      961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag
     1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
     1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac
     1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa
     1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg
     2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta
     2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
     2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
     2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
     2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
     2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
     2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
     2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
     2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
     3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
     3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
     3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc
     3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa
     3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc
     3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac
     3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca
     3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
     3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
     3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
     3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
     3781 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
     3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
     3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
     3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
     4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
     4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
     4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga
     4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
     4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
     4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
     4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
     4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
     4981 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa
     5041 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
     5101 atggtggacc cggtgggctt cgcggaggcg tggaaggcgc agttcccgga ctcagagccc
     5161 ccgcgcatgg agctgcgctc agtgggcgac atcgagcagg agctggagcg ctgcaaggcc
     5221 tccattcggc gcctggagca ggaggtgaac caggagcgct tccgcatgat ctacctgcag
     5281 acgttgctgg ccaaggaaaa gaagagctat gaccggcagc gatggggctt ccggcgcgcg
     5341 gcgcaggccc ccgacggcgc ctccgagccc cgagcgtccg cgtcgcgccc gcagccagcg
     5401 cccgccgacg gagccgaccc gccgcccgcc gaggagcccg aggcccggcc cgacggcgag
     5461 ggttctccgg gtaaggccag gcccgggacc gcccgcaggc ccggggcagc cgcgtcgggg
     5521 gaacgggacg accggggacc ccccgccagc gtggcggcgc tcaggtccaa cttcgagcgg
     5581 atacgcaagg gccatggcca gcccggggcg gacgccgaga agcccttcta cgtgaacgtc
     5641 gagtttcacc acgagcgcgg cctggtgaag gtcaacgaca aagaggtgtc ggaccgcatc
     5701 agctccctgg gcagccaggc catgcagatg gagcgcaaaa agtcccagca cggcgcgggc
     5761 tcgagcgtgg gggatgcatc caggccccct taccggggac gctcctcgga gagcagctgc
     5821 ggcgtcgacg gcgactacga ggacgccgag ttgaaccccc gcttcctgaa ggacaacctg
     5881 atcgacgcca atggcggtag caggccccct tggccgcccc tggagtacca gccctaccag
     5941 agcatctacg tcgggggcat gatggaaggg gagggcaagg gcccgctcct gcgcagccag
     6001 agcacctctg agcaggagaa gcgccttacc tggccccgca ggtcctactc cccccggagt
     6061 tttgaggatt gcggaggcgg ctataccccg gactgcagct ccaatgagaa cctcacctcc
     6121 agcgaggagg acttctcctc tggccagtcc agccgcgtgt ccccaagccc caccacctac
     6181 cgcatgttcc gggacaaaag ccgctctccc tcgcagaact cgcaacagtc cttcgacagc
     6241 agcagtcccc ccacgccgca gtgccataag cggcaccggc actgcccggt tgtcgtgtcc
     6301 gaggccacca tcgtgggcgt ccgcaagacc gggcagatct ggcccaacga tggcgagggc
     6361 gccttccatg gagacgcaga tggctcgttc ggaacaccac ctggatacgg ctgcgctgca
     6421 gaccgggcag aggagcagcg ccggcaccaa gatgggctgc cctacattga tgactcgccc
     6481 tcctcatcgc cccacctcag cagcaagggc aggggcagcc gggatgcgct ggtctcggga
     6541 gccctggagt ccactaaagc gagtgagctg gacttggaaa agggcttgga gatgagaaaa
     6601 tgggtcctgt cgggaatcct ggctagcgag gagacttacc tgagccacct ggaggcactg
     6661 ctgctgccca tgaagccttt gaaagccgct gccaccacct ctcagccggt gctgacgagt
     6721 cagcagatcg agaccatctt cttcaaagtg cctgagctct acgagatcca caaggagttc
     6781 tatgatgggc tcttcccccg cgtgcagcag tggagccacc agcagcgggt gggcgacctc
     6841 ttccagaagc tggccagcca gctgggtgtg taccgggcct tcgtggacaa ctacggagtt
     6901 gccatggaaa tggctgagaa gtgctgtcag gccaatgctc agtttgcaga aatctccgag
     6961 aacctgagag ccagaagcaa caaagatgcc aaggatccaa cgaccaagaa ctctctggaa
     7021 actctgctct acaagcctgt ggaccgtgtg acgaggagca cgctggtcct ccatgacttg
     7081 ctgaagcaca ctcctgccag ccaccctgac caccccttgc tgcaggacgc cctccgcatc
     7141 tcacagaact tcctgtccag catcaatgag gagatcacac cccgacggca gtccatgacg
     7201 gtgaagaagg gagagcaccg gcagctgctg aaggacagct tcatggtgga gctggtggag
     7261 ggggcccgca agctgcgcca cgtcttcctg ttcaccgacc tgcttctctg caccaagctc
     7321 aagaagcaga gcggaggcaa aacgcagcag tatgactgca aatggtacat tccgctcacg
     7381 gatctcagct tccagatggt ggatgaactg gaggcagtgc ccaacatccc cctggtgccc
     7441 gatgaggagc tggacgcttt gaagatcaag atctcccaga tcaagagtga catccagaga
     7501 gagaagaggg cgaacaaggg cagcaaggct acggagaggc tgaagaagaa gctgtcggag
     7561 caggagtcac tgctgctgct tatgtctccc agcatggcct tcagggtgca cagccgcaac
     7621 ggcaagagtt acacgttcct gatctcctct gactatgagc gtgcagagtg gagggagaac
     7681 atccgggagc agcagaagaa gtgtttcaga agcttctccc tgacatccgt ggagctgcag
     7741 atgctgacca actcgtgtgt gaaactccag actgtccaca gcattccgct gaccatcaat
     7801 aaggaagatg atgagtctcc ggggctctat gggtttctga atgtcatcgt ccactcagcc
     7861 actggattta agcagagttc aaaagccctt cagcggccag tagcatctga ctttgagcct
     7921 cagggtctga gtgaagccgc tcgttggaac tccaaggaaa accttctcgc tggacccagt
     7981 gaaaatgacc ccaacctttt cgttgcactg tatgattttg tggccagtgg agataacact
     8041 ctaagcataa ctaaaggtga aaagctccgg gtcttaggct ataatcacaa tggggaatgg
     8101 tgtgaagccc aaaccaaaaa tggccaaggc tgggtcccaa gcaactacat cacgccagtc
     8161 aacagtctgg agaaacactc ctggtaccat gggcctgtgt cccgcaatgc cgctgagtat
     8221 ctgctgagca gcgggatcaa tggcagcttc ttggtgcgtg agagtgagag cagtcctggc
     8281 cagaggtcca tctcgctgag atacgaaggg agggtgtacc attacaggat caacactgct
     8341 tctgatggca agctctacgt ctcctccgag agccgcttca acaccctggc cgagttggtt
     8401 catcatcatt caacggtggc cgacgggctc atcaccacgc tccattatcc agccccaaag
     8461 cgcaacaagc ccactgtcta tggtgtgtcc cccaactacg acaagtggga gatggaacgc
     8521 acggacatca ccatgaagca caagctgggc gggggccagt acggggaggt gtacgagggc
     8581 gtgtggaaga aatacagcct gacggtggcc gtgcggacct tgaaggagga caccatggag
     8641 gtggaagagt tcttgaaaga agctgcagtc atgaaagaga tcaaacaccc taacctggtg
     8701 cagctccttg gggtctgcac ccgggagccc ccgttctata tcatcactga gttcatgacc
     8761 tacgggaacc tcctggacta cctgagggag tgcaaccggc aggaggtgaa cgccgtggtg
     8821 ctgctgtaca tggccactca gatctcgtca gccatggagt acctggagaa gaaaaacttc
     8881 atccacagag atcttgctgc ccgaaactgc ctggtagggg agaaccactt ggtgaaggta
     8941 gctgattttg gcctgagcag gttgatgaca ggggacacct acacagccca tgctggagcc
     9001 aagttcccca tcaaatggac tgcacccgag agcctggcct acaacaagtt ctccatcaag
     9061 tccgacgtct gggcatttgg agtattgctt tgggaaattg ctacctatgg catgtcccct
     9121 tacccgggaa ttgacctgtc ccaggtgtat gagctgctag agaaggacta ccgcatggag
     9181 cgcccagaag gctgcccaga gaaggtctat gaactcatgc gagcatgttg gcagtggaat
     9241 ccctctgacc ggccctcctt tgctgaaatc caccaagcct ttgaaacaat gttccaggaa
     9301 tccagtatct cagacgaagt ggaaaaggag ctggggaaac aaggcgtccg tggggctgtg
     9361 agtaccttgc tgcaggcccc agagctgccc accaagacga ggacctccag gagagctgca
     9421 gagcacagag acaccactga cgtgcctgag atgcctcact ccaagggcca gggagagagc
     9481 gatcctctgg accatgagcc tgccgtgtct ccattgctcc ctcgaaaaga gcgaggtccc
     9541 ccggagggcg gcctgaatga agatgagcgc cttctcccca aagacaaaaa gaccaacttg
     9601 ttcagcgcct tgatcaagaa gaagaagaag acagccccaa cccctcccaa acgcagcagc
     9661 tccttccggg agatggacgg ccagccggag cgcagagggg ccggcgagga agagggccga
     9721 gacatcagca acggggcact ggctttcacc cccttggaca cagctgaccc agccaagtcc
     9781 ccaaagccca gcaatggggc tggggtcccc aatggagccc tccgggagtc tgggggctca
     9841 ggcttccggt ctccccacct gtggaagaag tccagcacgc tgaccagcag ccgcctagcc
     9901 accggcgagg aggagggcgg tggcagctcc agcaagcgct tcctgcgctc ttgctccgcc
     9961 tcctgcgttc cccatggggc caaggacacg gagtggaggt cagtcacgct gcctcgggac
    10021 ttgcagtcca cgggaagaca gtttgactcg tccacatttg gagggcacaa aagtgagaag
    10081 ccggctctgc ctcggaagag ggcaggggag aacaggtctg accaggtgac ccgaggcaca
    10141 gtaacgcctc cccccaggct ggtgaaaaag aatgaggaag ctgctgatga ggtcttcaaa
    10201 gacatcatgg agtccagccc gggctccagc ccgcccaacc tgactccaaa acccctccgg
    10261 cggcaggtca ccgtggcccc tgcctcgggc ctcccccaca aggaagaagc tggaaagggc
    10321 agtgccttag ggacccctgc tgcagctgag ccagtgaccc ccaccagcaa agcaggctca
    10381 ggtgcaccag ggggcaccag caagggcccc gccgaggagt ccagagtgag gaggcacaag
    10441 cactcctctg agtcgccagg gagggacaag gggaaattgt ccaggctcaa acctgccccg
    10501 ccgcccccac cagcagcctc tgcagggaag gctggaggaa agccctcgca gagcccgagc
    10561 caggaggcgg ccggggaggc agtcctgggc gcaaagacaa aagccacgag tctggttgat
    10621 gctgtgaaca gtgacgctgc caagcccagc cagccgggag agggcctcaa aaagcccgtg
    10681 ctcccggcca ctccaaagcc acagtccgcc aagccgtcgg ggacccccat cagcccagcc
    10741 cccgttccct ccacgttgcc atcagcatcc tcggccctgg caggggacca gccgtcttcc
    10801 accgccttca tccctctcat atcaacccga gtgtctcttc ggaaaacccg ccagcctcca
    10861 gagcggatcg ccagcggcgc catcaccaag ggcgtggtcc tggacagcac cgaggcgctg
    10921 tgcctcgcca tctctaggaa ctccgagcag atggccagcc acagcgcagt gctggaggcc
    10981 ggcaaaaacc tctacacgtt ctgcgtgagc tatgtggatt ccatccagca aatgaggaac
    11041 aagtttgcct tccgagaggc catcaacaaa ctggagaata atctccggga gcttcagatc
    11101 tgcccggcga cagcaggcag tggtccagcg gccactcagg acttcagcaa gctcctcagt
    11161 tcggtgaagg aaatcagtga catagtgcag aggtagctcg agcaccacca ccaccaccac
    11221 tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag
    11281 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa
    11341 ggaggaacta tatccggat
//
