LOCUS       V014982                 5485 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014982
VERSION     V014982
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             641..1423
                     /gene="CA1"
                     /label="Carbonic anhydrase 1"
                     /note="Carbonic anhydrase 1 from Homo sapiens. Accession#:
                     P00915"
     CDS             1472..2149
                     /label="DsRed1"
                     /note="wild-type DsRed"
     polyA_signal    2273..2394
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2401..2856)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2883..2987
                     /label="AmpR promoter"
     promoter        2989..3346
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3381..4172
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4407..4454
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4783..5371
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attcgccacc atggcaagtc cagactgggg
      661 atatgatgac aaaaatggtc ctgaacaatg gagcaagctg tatcccattg ccaatggaaa
      721 taaccagtcc cctgttgata ttaaaaccag tgaaaccaaa catgacacct ctctgaaacc
      781 tattagtgtc tcctacaacc cagccacagc caaagaaatt atcaatgtgg ggcattcctt
      841 ccatgtaaat tttgaggaca acgataaccg atcagtgctg aaaggtggtc ctttctctga
      901 cagctacagg ctctttcagt tccattttca ctggggcagt acaaatgagc atggttcaga
      961 acatacagtg gatggagtca aatattctgc cgagcttcac gtagctcact ggaattctgc
     1021 aaagtactcc agccttgctg aagctgcctc aaaggctgat ggtttggcag ttattggtgt
     1081 tttgatgaag gttggtgagg ccaacccaaa gctgcagaaa gtacttgatg ccctccaagc
     1141 aattaaaacc aagggcaaac gagccccatt cacaaatttt gacccctcta ctctccttcc
     1201 ttcatccctg gatttctgga cctaccctgg ctctctgact catcctcctc tttatgagag
     1261 tgtaacttgg atcatctgta aggagagcat cagtgtcagc tcagagcagc tggcacaatt
     1321 ccgcagcctt ctatcaaatg ttgaaggtga taacgctgtc cccatgcagc acaacaaccg
     1381 cccaacccaa cctctgaagg gcagaacagt gagagcttca tttggtggag gcggatcagg
     1441 aggcggtgga tcggatccac cggtcgccac catggtgcgc tcctccaaga acgtcatcaa
     1501 ggagttcatg cgcttcaagg tgcgcatgga gggcaccgtg aacggccacg agttcgagat
     1561 cgagggcgag ggcgagggcc gcccctacga gggccacaac accgtgaagc tgaaggtgac
     1621 caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttcc agtacggctc
     1681 caaggtgtac gtgaagcacc ccgccgacat ccccgactac aagaagctgt ccttccccga
     1741 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
     1801 ggactcctcc ctgcaggacg gctgcttcat ctacaaggtg aagttcatcg gcgtgaactt
     1861 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg
     1921 cctgtacccc cgcgacggcg tgctgaaggg cgagatccac aaggccctga agctgaagga
     1981 cggcggccac tacctggtgg agttcaagtc catctacatg gccaagaagc ccgtgcagct
     2041 gcccggctac tactacgtgg actccaagct ggacatcacc tcccacaacg aggactacac
     2101 catcgtggag cagtacgagc gcaccgaggg ccgccaccac ctgttcctgt agcggccgcg
     2161 actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     2221 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     2281 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     2341 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaaggcgt
     2401 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     2461 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     2521 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     2581 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     2641 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     2701 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     2761 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     2821 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg
     2881 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     2941 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa
     3001 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     3061 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     3121 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     3181 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     3241 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     3301 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga
     3361 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     3421 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     3481 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     3541 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg
     3601 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     3661 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     3721 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     3781 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     3841 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     3901 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     3961 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     4021 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     4081 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     4141 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     4201 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     4261 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     4321 tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac
     4381 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt
     4441 tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc
     4501 accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc
     4561 aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc
     4621 ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     4681 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     4741 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     4801 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     4861 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     4921 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     4981 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5041 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5101 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     5161 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     5221 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     5281 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     5341 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     5401 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     5461 ggataaccgt attaccgcca tgcat
//
