LOCUS       62056_20550        9883 bp DNA     circular SYN 06-APR-2019
DEFINITION  Transformation vector pT67KV, complete sequence.
ACCESSION   MK424968
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9883)
  AUTHORS   Luo J, Shen P, Chen J.
  TITLE     A modular toolset of phiC31-based fluorescent protein tagging 
            vectors for Drosophila
  JOURNAL   Fly (Austin) (2019) In press
  PUBMED    30885036
REFERENCE   2  (bases 1 to 9883)
  AUTHORS   Luo J.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2019) Model Animal Research Center, Nanjing 
            University, 12 Xu-fu Rd Pu-kou Dist, Nanjing, Jiangsu 210061, China
REFERENCE   3  (bases 1 to 9883)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fly 
            (Austin) (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-JAN-2019) Model Animal Research Center, Nanjing University, 12 
            Xu-fu Rd Pu-kou Dist, Nanjing, Jiangsu 210061, China"
FEATURES             Location/Qualifiers
     source          1..9883
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    667..2546
                     /label=vermilion
                     /note="vermilion"
     protein_bind    2553..2586
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    2587..3017
                     /label=gypsy insulator
                     /note="chromatin insulator from Drosophila"
     misc_feature    complement(3027..4407)
                     /label=K10 polyA signal sequence
                     /note="K10 polyA signal sequence"
     misc_feature    complement(4457..4480)
                     /label=multiple cloning site
                     /note="multiple cloning site"
     misc_feature    complement(4983..4985)
                     /label=ATG
                     /note="ATG"
     misc_feature    complement(4986..6903)
                     /label=aTub67C promoter
                     /note="aTub67C promoter"
     misc_feature    6938..7368
                     /label=gypsy insulator
                     /note="gypsy insulator"
     protein_bind    7407..7476
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     promoter        complement(7695..7713)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7734..7750)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7758..7774)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7782..7812)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7827..7848)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8136..8724)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8898..9755)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9756..9860)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggccccattt gtgtgcgccg cactttcacc tcaagtgatt gataattccc agcctatctg
      721 gcagtgccca tcgcccagat caccgactgt gcaatcagtc ggaactggag ctctctcgct
      781 ctgttatcgg ttcgctgggg tctcatctcc ggtccgctgg cggagatcag ttcgccagca
      841 tccgccgctc gaggagtcac gatctgatct gagctgtgca ccatgagctg tccctatgca
      901 ggaaacgggt gagcaccagc acgtgctgtc caggaatgcc aatcgatctt cagttctgcg
      961 attcaattca aacccataca gaaacgatca cgatgattcg gcggtgccat taaccacgga
     1021 agtgggcaaa atctatggag agtatctgat gctggacaaa ctgctggatg cccagtgtat
     1081 gctgtccgag gaggacaagc gacccgtgca cgatgagcat ctgttcatca tcacgcacca
     1141 gggtgagtag gtttacaact ttgatgacaa cactcaatgg catttaagta ccttcgccac
     1201 agcctacgag ctttggttca agcagatcat ctttgagttc gactccatac gagacatgtt
     1261 ggatgcagag gtcatcgatg aaaccaagac gctggagatt gtcaagcgac tgaaccgagt
     1321 ggttctgatt ctaaaagtga gtgctttctg aatctcttac caaaatccgt ttataacttc
     1381 ctttgtacag ctcctggtgg accaagtgcc cattctggag accatgaccc cgctagactt
     1441 catggacttc cgcaagtacc tggcacccgc atctggtttt cagtcgctgc agttccgttt
     1501 gatcgagaac aagctgggag ttctgacaga gcagcgggtg agatacaacc agaagtactc
     1561 ggatgtcttt agcgacgagg aggcgcggaa ctcgattcgc aactcggaga aagatccctc
     1621 gctactggag ctagtgcagc gatggctgga gaggacgccc ggactggagg agagtggctt
     1681 caacttctgg gccaagtttc aggagagcgt cgatcgattc ctggaggcgc aggtacagag
     1741 cgccatggag gagcccgtgg agaaggcgaa aaactaccgc ctcatggaca ttgagaagcg
     1801 acgcgaggtg tatcgctcca tctttgatcc ggcagtgcac gatgcactgg tgcgtcgtgg
     1861 ggatcgccgg tttagccatc gtgcccttca gggagccatc atgatcacct tctataggga
     1921 tgaacccagg ttcagccaac cacaccagtt gctcaccctg ctcatggaca tcgactcgtt
     1981 aataaccaag tggagatgta agtattgcat tctttgatac tcttttataa atatatctta
     2041 tgtttaagac tggttttcct aaccaaatac tttctattcc cgccgcagac aatcacgtga
     2101 tcatggtgca acgcatgatt ggatcccaac agttgggcac tggtggctcg tctggatatc
     2161 aatatctgcg ctccactctc aggtgatcat cgcagatgtg attatatcgg ggatcaatga
     2221 actcaaactg ttctcccttt gttttttttt ggtttcagtg atcggtacaa ggtgtttctg
     2281 gatctgttca atctgtccac ttttctgatt ccccgcgagg cgattccacc gctggacgag
     2341 accattcgca agaaactgat caacaaaagt gtctgacaat cggcagggta tccaattcgt
     2401 caatgtttgg ctatgcgttg tttgttctgc ctactgtttt gtcgttttgg tgtaataaaa
     2461 ttacttgttt agtctttgtt atcacatttg atgtgttcct tttctttatg tctgacatat
     2521 aatacatata acataacaaa ataaatgcta gtataacttc gtataatgta tgctatacga
     2581 agttattggc cacgtaataa gtgtgcgttg aatttattcg caaaaacatt gcatattttc
     2641 ggcaaagtaa aattttgttg cataccttat caaaaaataa gtgctgcata ctttttagag
     2701 aaaccaaata attttttatt gcatacccgt ttttaataaa atacattgca taccctcttt
     2761 taataaaaaa tattgcatac tttgacgaaa caaattttcg ttgcataccc aataaaagat
     2821 tattatattg catacccgtt tttaataaaa tacattgcat accctctttt aataaagaat
     2881 attgcatacg ttgacgaaac aaattttcgt tgcataccca ataaaagatt attatattgc
     2941 ataccttttc ttgccatacc atttagccga tcaattgtgc tcggcaacag tatatttgtg
     3001 gtgtgccaac caacaacacg cgtacttcag ccaatccgcc gcaccctcag ctccaaagca
     3061 cgcatatgac ccgtgagcag gcagagaaac gcaggcggat aggagtcact agcgcagttc
     3121 tgtatagcct gcactatcaa gccgaagagc tggtagatat tgatgagcac atagtttctg
     3181 gtggagtgca tccagtcaac gccgaaccag gccggataca ggagctctcg atccgggcga
     3241 acgaccacgc ggacgcaact caaagtagtg ccaaatacga aaatactggc aaaggtgcgg
     3301 aaagtgccct gtgcgatatt cacttccttc ctcaaggcag aaatctcctc gggatcgccc
     3361 accagccggg ctctagcgtc catgagcctt aggagagagc gaatctcatg gagctgcttc
     3421 ctcaccatgt acacattcgc aaacttcaaa ttggccacaa tatcggtaat agttatggtc
     3481 aggttctcgc acaatccggc catgttggtg gtgaacagca gcctggccag caagctcaag
     3541 ggaaacagca cggtgaccac caagttgacc gtaatggagt ataccacgta aagcaggctt
     3601 gaaacgcccg gaggacgcat caggccagtg agctcccaaa cgcgccagtg gtagtacaca
     3661 gcactgtggg tgttcagttt gaaatcctct tgcttctcca ttgtctcggt tacctttggt
     3721 caaatccatg ggttctattg cctatatact cttgcgatta ccagtgattg cgctattagc
     3781 tattagatgg attgttggcc aaacttgtcg cttaagtggc tgggaattgt aaccgtaggc
     3841 ccgagtgtaa tgatccccca taaaaagttt tcgcaatgcc tttatttttt gttgcaaatc
     3901 tctctttatt ctgcggtatt cttcattatt gcggggatgg ggaaagtgtt tatatagaag
     3961 caacttacga ttgaacccaa atgcacctga caagcaaggt caaagggcca gatttttaaa
     4021 tatattattt agtcttagga ctctctattt gcaattaaat tactttgcta cctgagggtt
     4081 aaatcttccc cattgataat aataattcca ctatatgttc aattgggttt caccgcgctt
     4141 agttacatga cgagccctaa tgagccgtcg gtggtctata aactgtgcct tacaaatact
     4201 tgcaactctt ctcgttttga agtcagcaga gttattgcta attgctaatt gctaattgct
     4261 tttaactgat ttcttcgaaa ttggtgctat gtttatggcg ctattaacaa gtatgaatgt
     4321 caggtttaac caggggatgc ttaattgtgt tctcaacttc aaaggcagaa atgtttactc
     4381 ttgaccatgg gtttaggtat aatgttatca agctcctcga gttaacgtta cgttaacgtt
     4441 aacgttcgag gtcgacgcta gctccgcggc cgccggtacc aatctggatg gagactactt
     4501 cgcgctggcg ggtgaaacaa taaaagtgat gagctgggca agagaaggcg ccattgttta
     4561 aggttaggtt aggtcctccc tttgtaattt actttataaa cacattacta actaaaacta
     4621 atattaagaa cagtattata tgacgaaaaa gtagcactct tttttataaa tggaatattt
     4681 tatcactcaa aaaatatttt aaattaaaga ttctttaata aacttgattg tctgttataa
     4741 tacaaacgag gaatttgttt taaataaaca attcaaagat cttaatattt ttcaaagatt
     4801 tgtaagcagt aacagttaag acgtttcaaa aaattcgcaa ttagctgttc atttccaatt
     4861 aacatttatt catgttcact tcccacacct tttcatttca catcttacac atcttttctg
     4921 attttaaatc tcacttcccg cttttcgcaa ctcttaaact aattggtttc ttttcttctc
     4981 accattttat tagttttttt tgctggacta aaattgggtg tatacaaatt aaaatctaat
     5041 gcgggacctt ttttttataa tttttttggt cgccacgcga aaagtgcagc ctcaggttag
     5101 ttggttcgct gattggggag tcactggaaa agcgccgtta cagagcgcca aagaggaatg
     5161 tcaaattggg agacagggct gctaaccaat aatcattcca aggtttttcc aacccatttc
     5221 tggcggctta tcgatactaa tttgaaatag tatgtatgta tctcaggatt tgctttacct
     5281 ttacttaaat tattattaaa aactatataa tatcatattg aaaatcttca aaagtttagg
     5341 tgtcttcaaa acttgtttat ttttacaaat tcatatattg acaaatgcac tctacttgaa
     5401 taaatatttt atggcatctt aagtgtgaat ttcttttttt aatttcgtgc gaattgaatt
     5461 ctatctctct agacacctac aattgtgctt caaaatcttt acagacgaaa taccttaaca
     5521 taaacataaa gtgggacgaa aataaattca ttgaacctgt gacacaataa ataatcatct
     5581 aggcatgcta atccttgcga aatccggggg gcagcgtcac atcgaaaacg atgggggcat
     5641 tgttgggtag gtagttgatg tcacacgttg aggcattcac aaagatgatc ctgccatcgc
     5701 tggtgatgcc gtagccttcg tgtacatgtc cgaagacatg gtatttcggg cgcactcgtt
     5761 gctgcactgt gctgagtagc tccacacaac cggctcgtac gccggaacag cagagatcgc
     5821 cgtgacctac gggcggcgtg tgggtgacca ggatatcgat gccttccggg atttgattcc
     5881 acttatccaa acaggcagtt ccacgtggaa cattaaaggc ccagcgacag aattccggtt
     5941 gccatggcga gccatatatt tgaatacccc aaatctcaag tagttcatcc tccaaatacc
     6001 gacagttggt cagaacatcg cggacattct gcgtttgcac agcgctctcc aagttctcct
     6061 tggcgttgcc caaagtgggc agatcatcga ggatggacat gccggtgtgt ttgctgctcg
     6121 aagcatgact tttgctcttc tggaaaggat gggtgaaggt tctatcgaag ctgagctcat
     6181 gattgccagc aatcacgatc ttgtggcgat ggggcagtgc tccaatccag gtgttaaact
     6241 cctccacctc ctccagttgg ccgcacttgg taaaatcgcc ggcatgtatg aatatatcgc
     6301 catctggtat atcgaacttg atgtagggcg tcagggagtg cgtgtcggac atgcaaacca
     6361 ctcgtgcctt gttgggcgcc accgtggtgg tcggtggctt cattgtcacc ttgatcacgc
     6421 gctgcgtttt gctaatctcc ctccaagcgg ccgtgggatc ctggctaagt ggatgtactg
     6481 gcacttccat tttgatatct gcaatggtgg cgtgtgagga ttactatgaa ttttcttatg
     6541 gagaactggt ttgtttacgt ataattcagc gaggtattta aacacagata ctaaatcttt
     6601 tgtttaagat agtaagagct atacataaga cgtggccatg aatgttatct tactaagaca
     6661 gttctttaat tttaaagtac tgggaattat gggtttttcg tgtgattcgt aaatttcata
     6721 tattaaaata ttcaatgcaa ttacatttgt gttaaaagga caggcacttg aaaaagattt
     6781 aatgagtgtt gtagttaagc caatataatt ggcgtacgtt ccaatttagg aagcatttgt
     6841 aactatatct aaagagtaga gttaaaagtc acaactaata caggtaacag cttcatttgt
     6901 caacctgcag gcatgcaagc ttggatcctc aaggccttgg ccacgtaata agtgtgcgtt
     6961 gaatttattc gcaaaaacat tgcatatttt cggcaaagta aaattttgtt gcatacctta
     7021 tcaaaaaata agtgctgcat actttttaga gaaaccaaat aattttttat tgcatacccg
     7081 tttttaataa aatacattgc ataccctctt ttaataaaaa atattgcata ctttgacgaa
     7141 acaaattttc gttgcatacc caataaaaga ttattatatt gcatacccgt ttttaataaa
     7201 atacattgca taccctcttt taataaagaa tattgcatac gttgacgaaa caaattttcg
     7261 ttgcataccc aataaaagat tattatattg catacctttt cttgccatac catttagccg
     7321 atcaattgtg ctcggcaaca gtatatttgt ggtgtgccaa ccaacaacac tagagatcca
     7381 ctagtgtcga cgatgtaggt cacggtctcg aagccgcggt gcgggtgcca gggcgtgccc
     7441 ttgggctccc cgggcgcgta ctccacctca cccatctggt ccatcatgat gaacgggtcg
     7501 aggtggcggt agttgatccc ggcgaacgcg cggcgcaccg ggaagccctc gccctcgaaa
     7561 ccgctgggcg cggtggtcac ggtgagcacg ggacgtgcga cggcgtcggc gggtgcggat
     7621 acgcggggca gcgtcagcgg gttctcgacg gtcacggcgg gcatgtcgac actagttcta
     7681 gccagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc atggtcatag
     7741 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     7801 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     7861 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     7921 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     7981 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     8041 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     8101 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     8161 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     8221 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     8281 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     8341 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     8401 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     8461 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     8521 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     8581 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     8641 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     8701 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     8761 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     8821 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     8881 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8941 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     9001 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     9061 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     9121 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     9181 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     9241 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     9301 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     9361 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     9421 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     9481 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     9541 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     9601 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     9661 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     9721 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     9781 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     9841 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cac
//
