LOCUS       62056_20545        4116 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     CDS             94..111
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             118..825
                     /codon_start=1
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     terminator      863..957
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             1001..1657
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      1725..1811
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1966..2106
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2292..2880)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3054..3911)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3912..4016)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttccatcacc atcaccatca cggatccatg
      121 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
      181 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
      241 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
      301 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
      361 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
      421 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
      481 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
      541 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
      601 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
      661 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
      721 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
      781 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaggg tacctgcagc
      841 caagcttaat tagctgagct tggactcctg ttgatagatc cagtaatgac ctcagaactc
      901 catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg tgagaatcca
      961 agctagcttg gcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg
     1021 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc
     1081 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac
     1141 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat
     1201 gaatgctcat ccggaatttc gtatggcaat gaaagacggt gagctggtga tatgggatag
     1261 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag
     1321 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta
     1381 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc
     1441 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt
     1501 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct
     1561 ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga
     1621 attacaacag tactgcgatg agtggcaggg cggggcgtaa tttttttaag gcagttattg
     1681 gtgcccttaa acgcctgggg taatgactct ctagcttgag gcatcaaata aaacgaaagg
     1741 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
     1801 gtaggacaaa tccgccctct agagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
     1861 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     1921 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
     1981 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta
     2041 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     2101 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     2161 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     2221 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2281 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2341 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2401 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2461 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     2521 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     2581 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     2641 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     2701 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     2761 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     2821 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     2881 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     2941 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     3001 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     3061 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     3121 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     3181 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     3241 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     3301 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     3361 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     3421 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     3481 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     3541 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     3601 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     3661 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     3721 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     3781 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     3841 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     3901 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     3961 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     4021 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     4081 aaaaataggc gtatcacgag gccctttcgt cttcac
//
