LOCUS       40924_42299       10329 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pT3C, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10329)
  AUTHORS   Li XL, Skory CD, Ximenes EA, Jordan DB, Dien BS, Hughes SR, Cotta 
            MA.
  TITLE     Expression of an AT-rich xylanase gene from the anaerobic fungus 
            Orpinomyces sp. strain PC-2 in and secretion of the heterologous 
            enzyme by Hypocrea jecorina
  JOURNAL   Appl. Microbiol. Biotechnol. 74 (6), 1264-1275 (2007)
  PUBMED    17225100
REFERENCE   2  (bases 1 to 10329)
  AUTHORS   Li X-L.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-NOV-2006) Fermentation Biotechnology Research Unit, 
            National Center for Agricultural Utilization Research, USDA-ARS, 
            1815 N. University Street, Peoria, IL 61604, USA
REFERENCE   3  (bases 1 to 10329)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10329)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Microbiol. Biotechnol."; date: "2007"; volume: "74"; issue: "6"; 
            pages: "1264-1275"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-NOV-2006) Fermentation Biotechnology Research Unit, National 
            Center for Agricultural Utilization Research, USDA-ARS, 1815 N. 
            University Street, Peoria, IL 61604, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        21..125
                     /label=AmpR promoter
     CDS             126..983
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1157..1745
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2033..2054
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2069..2099
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2107..2123
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2131..2147
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2168..2186
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      2206..4420
                     /label=from Hypocrea jecorina cel7A
                     /note="from Hypocrea jecorina cel7A"
                     /regulatory_class="promoter"
     RBS             3933..3941
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(4559..4570)
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     regulatory      4702..5410
                     /label=from Hypocrea jecorina cel7A
                     /note="from Hypocrea jecorina cel7A"
                     /regulatory_class="terminator"
     promoter        5420..5780
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             5785..6807
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     misc_feature    6826..9648
                     /label=Hypocrea jecorina cel7A 3' region
                     /note="Hypocrea jecorina cel7A 3' region"
     promoter        complement(9686..9704)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9711..9727)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      9869..10324
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
       61 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
      121 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      181 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
      241 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
      301 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      361 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      421 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      481 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      541 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      601 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      661 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      721 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      781 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      841 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      901 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      961 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     1021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     1081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     1141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     1201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     1321 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1381 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1441 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1501 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1561 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1621 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1681 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1741 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1801 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     1861 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     1921 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     1981 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     2041 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     2101 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     2161 agctcgaaat taaccctcac taaagggaac aaaagctggg tacccgaatt ctcacggtga
     2221 atgtaggcct tttgtagggt aggaattgtc actcaagcac ccccaacctc cattacgcct
     2281 cccccataga gttcccaatc agtgagtcat ggcactgttc tcaaatagat tggggagaag
     2341 ttgacttccg cccagagctg aaggtcgcac aaccgcatga tatagggtcg gcaacggcaa
     2401 aaaagcacgt ggctcaccga aaagcaagat gtttgcgatc taacatccag gaacctggat
     2461 acatccatca tcacgcacga ccactttgat ctgctggtaa actcgtattc gccctaaacc
     2521 gaagtgcgtg gtaaatctac acgtgggccc ctttcggtat actgcgtgtg tcttctctag
     2581 gtgccattct tttcccttcc tctagtgttg aattgtttgt gttggagtcc gagctgtaac
     2641 tacctctgaa tctctggaga atggtggact aacgactacc gtgcacctgc atcatgtata
     2701 taatagtgat cctgagaagg ggggtttgga gcaatgtggg actttgatgg tcatcaaaca
     2761 aagaacgaag acgcctcttt tgcaaagttt tgtttcggct acggtgaaga actggatact
     2821 tgttgtgtct tctgtgtatt tttgtggcaa caagaggcca gagacaatct attcaaacac
     2881 caagcttgct cttttgagct acaagaacct gtggggtata tatctagagt tgtgaagtcg
     2941 gtaatcccgc tgtatagtaa tacgagtcgc atctaaatac tccgaagctg ctgcgaaccc
     3001 ggagaatcga gatgtgctgg aaagcttcta gcgagcggct aaattagcat gaaaggctat
     3061 gagaaattct ggagacggct tgttgaatca tggcgttcca ttcttcgaca agcaaagcgt
     3121 tccgtcgcag tagcaggcac tcattcccga aaaaactcgg agattcctaa gtagcgatgg
     3181 aaccggaata atataatagg caatacattg agttgcctcg acggttgcaa tgcaggggta
     3241 ctgagcttgg acataactgt tccgtacccc acctcttctc aacctttggc gtttccctga
     3301 ttcagcgtac ccgtacaagt cgtaatcact attaacccag actgaccgga cgtgttttgc
     3361 ccttcatttg gagaaataat gtcattgcga tgtgtaattt gcctgcttga ccgactgggg
     3421 ctgttcgaag cccgaatgta ggattgttat ccgaactctg ctcgtagagg catgttgtga
     3481 atctgtgtcg ggcaggacac gcctcgaagg ttcacggcaa gggaaaccac cgatagcagt
     3541 gtctagtagc aacctgtaaa gccgcaatgc agcatcactg gaaaatacaa accaatggct
     3601 aaaagtacat aagttaatgc ctaaaggagt catataccag cggctaataa ttgtacaatc
     3661 aagtggctaa acgtaccgta atttgccaac ggcttgtggg gttgcagaag caacggcaaa
     3721 gccccacttc cccacgtttg tttcttcact cagtccaatc tcagctggtg atcccccaat
     3781 tgggtcgctt gtttgttccg gtgaagtgaa agaagacaga ggtaagaatg tctgactcgg
     3841 agcgttttgc atacaaccaa gggcagtgat ggaagacagt gaaatgttga cattcaagga
     3901 gtatttagcc agggatgctt gagtgtatcg tgtaaggagg tttgtctgcc gatacgacga
     3961 atactgtata gtcacttctg atgaagtggt ccatattgaa atgtaagtcg gcactgaaca
     4021 ggcaaaagat tgagttgaaa ctgcctaaga tctcgggccc tcgggccttc ggcctttggg
     4081 tgtacatgtt tgtgctccgg gcaaatgcaa agtgtggtag gatcgaacac actgctgcct
     4141 ttaccaagca gctgagggta tgtgataggc aaatgttcag gggccactgc atggtttcga
     4201 atagaaagag aagcttagcc aagaacaata gccgataaag atagcctcat taaacggaat
     4261 gagctagtag gcaaagtcag cgaatgtgta tatataaagg ttcgaggtcc gtgcctccct
     4321 catgctctcc ccatctactc atcaactcag atcctccagg agacttgtac accatctttt
     4381 gaggcacaga aacccaatag tcaaccgcgg actgcgcatc atgaacaagt ccgtggctcc
     4441 attgctgctt gcagcgtcca tactatatgg cggcgccgtc gcacagcaga ctgtctgggg
     4501 ccagtgtgga ggtattggtt ggagcggacc tacgaattgt gctcctggat cagcttgttc
     4561 gaccctcaat ccttattatg cgcaatgtat tccgggagcc actactatca ccacttcgac
     4621 ccggccacca tccggtccaa ccaccaccac cagggctacc tcaacaagct catcaactcc
     4681 acccactagt gtgctgcagc cactaccact ggaagctctc ccggacctac ccagtctcac
     4741 tacggccagt gcggcggtat tggctacagc ggccccacgg tctgcgccag cggcacaact
     4801 tgccaggtcc tgaaccctta ctactctcag tgcctgtaaa gctccgtggc gaaagcctga
     4861 cgcaccggta gattcttggt gagcccgtat catgacggcg gcgggagcta catggccccg
     4921 ggtgatttat tttttttgta tctacttctg acccttttca aatatacggt caactcatct
     4981 ttcactggag atgcggcctg cttggtattg cgatgttgtc agcttggcaa attgtggctt
     5041 tcgaaaacac aaaacgattc cttagtagcc atgcatttta agataacgga atagaagaaa
     5101 gaggaaatta aaaaaaaaaa aaaaacaaac atcccgttca taacccgtag aatcgccgct
     5161 cttcgtgtat cccagtacca cggcaaaggt atttcatgat cgttcaatgt tgatattgtt
     5221 cccgccagta tggctccacc ccccatctcc gcgaatctcc tcttctcgaa cgcggtagtg
     5281 gcgcgccaat tggtaatgac cccataggga gacaaacagc ataatagcaa cagtggaaat
     5341 tagtggcgca ataattgaga acacagtgag accatagctg gcggcctgga aagcactgtt
     5401 ggagaccaac ttgtcgacac agaagatgat attgaaggag cactttttgg gcttggctgg
     5461 agctagtgga ggtcaacaat gaatgcctat tttggtttag tcgtccaggc ggtgagcaca
     5521 aaatttgtgt cgtttgacaa gatggttcat ttaggcaact ggtcagatca gccccacttg
     5581 tagcagtagc ggcggcgctc gaagtgtgac tcttattagc agacaggaac gaggacatta
     5641 ttatcatctg ctgcttggtg cacgataact tggtgcgttt gtcaagcaag gtaagtgaac
     5701 gacccggtca taccttctta agttcgccct tcctcccttt atttcagatt caatctgact
     5761 tacctattct acccaagcat cgatatgaaa aagcctgaac tcaccgcgac gtctgtcgag
     5821 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa
     5881 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
     5941 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
     6001 ccgattccgg aagtgcttga cattgggcaa ttcagcgaga gcctgaccta ttgcatctcc
     6061 cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg
     6121 caaccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg
     6181 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc
     6241 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg
     6301 tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg
     6361 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca
     6421 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc
     6481 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg
     6541 catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac
     6601 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga
     6661 tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga
     6721 agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc
     6781 cccagcactc gtccgagggc aaaggaatag agtagatgcc gaccgcatgc ccatctccgc
     6841 gaatctcctc ttctcgaacg cggtagtggc gcgccaattg gtaatgaccc catagggaga
     6901 caaacagcat aatagcaaca gtggaaatta gtggcgcaat aattgagaac acagtgagac
     6961 catagctggc ggcctggaaa gcactgttgg agaccaactt gtccgttgcg aggccaactt
     7021 gcattgctgt caagacgatg acaacgtagc cgaggaccgt cacaagggac gcaaagttgt
     7081 cgcggatgag gtctccgtag atggcatagc cggcaatccg agagtagcct ctcaacaggt
     7141 ggccttttcg aaaccggtaa accttgttca gacgtcctag ccgcagctca ccgtaccagt
     7201 atcgaggatt gacggcagaa tagcagtggc tctccaggat ttgactggac aaaatcttcc
     7261 agtattccca ggtcacagtg tctggcagaa gtcccttctc gcgtgcgagt cgaaagtcgc
     7321 tatagtgcgc aatgagagca cagtaggaga ataggaaccc gcgagcacat tgttcaatct
     7381 ccacatgaat tggatgactg ctgggcagaa tgtgctgcct ccaaaatcct gcgtccaaca
     7441 gatactctgg caggggcttc agatgaatgc ctctgggccc ccagataaga tgcagctctg
     7501 gattctcggt tacgatgata tcgcgagaga gcacgagttg gtgatggagg ggacgaggag
     7561 gcataggtcg gccgcaggcc cataaccagt cttgcacagc attgatcttc ctcacgagga
     7621 gctcctgatg cagaaactcc tccatgttgc tgattgggtt gagaatttca tcgctcctgg
     7681 atcgtatggt tgctggcaag accctgctta accgtgccgt gtcatggtca tctctggtgg
     7741 cttcgtcgct ggcctgtctt tgcaattcga cagcaaatgg tggagatctc tctatcgtga
     7801 cagtcatggt agcgatagct aggtgtcgtt gcacgcacat aggccgaaat gcgaagtgga
     7861 aagaatttcc cggcgcggaa tgaagtctcg tcattttgta ctcgtactcg acacctccac
     7921 cgaagtgtta agaatggatc cacgatgcca aaaagcttgt tcatttcggc tagcccgtga
     7981 tcctggcgct tctagggctg aaactgtgtt gttaatgtat tattggctgt gtaactgact
     8041 tgaatgggga atgaggagcg cgatggattc gcttgcatgt cccctggcca agacgagccg
     8101 ctttggcggt ttgtgattcg aaggtgtgtc agcggaggcg ccagggcaac acgcactgag
     8161 ccagccaaca tgcattgctg ccgacatgaa tagacacgcg ccgagcagac ataggagacg
     8221 tgttgactgt aaaaattcta ctgaatatta gcacgcatgg tctcaataag rgcwataggm
     8281 atgcktgccm atcataagta cgtatgtgct ttttcckgcm aatggtacgt acggacagtt
     8341 catgttgtct gtcatcccsc actcaggctc tcatgatcat tttatgggac tggggttttg
     8401 ctgactgaat ggattcagcc gcacgaaaca aattgggggc catgcagaag ggaagccccc
     8461 ccagccccct gttcataatt tgttaagagt cggagagctg cctagtatga agcagcaatt
     8521 gataacgttg actttgcgca tgagctctga agccgggcat atgtatcacg tttctgccta
     8581 gagccgcacg ggacccaaga agctcttgtc ataaggtatt tatgagtgtt cagctgccaa
     8641 cgctggttct actttggctc aaccgcatcc cataagctga actttgggag ctgccagaat
     8701 gtctcttgat gtacagcgat caacaaccgt gcgccggtcg acaactgttc accgatcagg
     8761 gacgcgaaga ggacccaatc ccgcggttaa cgcacctgct ccgaagaagc aaaagggcta
     8821 tgaggtggtg cagcaaggaa tcaaagagct ctatccactt gacaaggcca atgtcgctcc
     8881 cgatctggag taagtcaacc ctgaagtgga agtttgcttc tctgattagt atgtagcatc
     8941 gtgtttgtcc caggactggg tgcaaatccc gaagacagct ggaagtccag caagaccgac
     9001 ttcaattgga ccacgcatac agatggcctc cagagagact tcccaagagc tcggttgctt
     9061 ctgtatatgt acgactcagc atggactggc cagctcaaag taaaacaatt catgggcaat
     9121 atcgcgatgg ggctcttggt tgggctgagg agcaagagag aggtaggcca aacgccagac
     9181 tcgaaccgcc agccaagtct caaactgact gcaggtcttc acagaaatgc caacggaggc
     9241 ccattgtgtt cataggacac agtatgggcg gtctagtggt tgccaaagcc atcactcttg
     9301 cagactctcg ccgcgatctt ttccccatca tgtttgaggc aacgacggcg tgcatattct
     9361 tcggaactcc gtttggcggc gcccctgttg cttctgtcgc agcaatgtat gcgcactttg
     9421 ctgagaaagt cgatgtggca ttttcatcca agttgttgga actgatgaag ccgggagatg
     9481 aagagctacg acagctcacg cacgatttca tgcgcttggc agggaagatc agcccgaaga
     9541 tcgaactgtt ttgcttttgg gaagagcacc caacagattt ctcaaagatg gctggccttc
     9601 caagtctgtt tgggctcacc aagtccctca taccaaagca gtatgccgaa ttcgcggccg
     9661 ccaccgcggt ggagctccaa ttcgccctat agtgagtcgt attacaattc actggccgtc
     9721 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     9781 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     9841 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     9901 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     9961 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
    10021 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
    10081 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
    10141 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
    10201 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
    10261 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
    10321 atttaggtg
//
