LOCUS       V041311                 6707 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V041311
VERSION     V041311
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        1..1179
                     /note="strong constitutive promoter for human elongation
                     factor EF-1α"
                     /label="EF-1α promoter"
     promoter        1196..1214
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     protein_bind    1293..1317
                     /bound_moiety="BP Clonase™"
                     /gene="mutant version of&nbsp;attB"
                     /note="recombination site for the Gateway® BP reaction"
                     /label="attB1"
     CDS             1342..2601
                     /label="RBM22(NM_018047)"
                     /note="RBM22(NM_018047)"
                     /gene="RBM22"
     polyA_signal    2635..2859
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     protein_bind    2929..2962
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
                     /label="loxP"
     primer_bind     complement(3420..3436)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     promoter        3910..4014
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             4015..4875
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      5046..5634
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     protein_bind    5922..5943
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        5958..5988
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5996..6012
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     6020..6036
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6534..6567
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
                     /label="loxP"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata
     1201 cgactcacta tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac
     1261 tagtccagtg tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcaggctccg
     1321 cggccgcccc cttcagccac catggcgacc tctctgggtt ccaacaccta caacaggcag
     1381 aactgggagg atgcggactt ccccattctg tgccagacat gtcttggaga aaacccatat
     1441 atccgaatga ccaaagaaaa gtatgggaag gaatgcaaaa tctgtgccag gccattcaca
     1501 gtgtttcgct ggtgccctgg agtccgcatg cgtttcaaga agactgaagt gtgccaaacc
     1561 tgcagtaaat tgaagaatgt ctgtcagacc tgcctcttag acctagagta tggcctgccc
     1621 atccaggttc gtgacgcagg attgtctttt aaagatgaca tgccaaagtc agatgtcaac
     1681 aaagagtact atacacagaa tatggagaga gagatttcta actctgatgg aacacggcca
     1741 gttggcatgc tggggaaagc cacatctacc agtgacatgc tgctcaaact ggcccggacc
     1801 acaccctact acaaaaggaa tcgaccccac atttgctcct tctgggtgaa aggagagtgt
     1861 aagagaggag aggaatgtcc atacagacat gagaagccta cagatccaga tgaccccctt
     1921 gctgatcaga atattaaaga ccgttattac ggaatcaatg atcctgtagc tgacaagctt
     1981 ctaaagcggg cttcaacaat gcctcggctg gacccaccag aggataaaac tatcaccaca
     2041 ctatatgttg gtggtctagg tgataccatt actgagacag atttaagaaa tcatttctac
     2101 cagttcggag agatccggac gatcactgtt gtgcagagac agcagtgtgc tttcatccag
     2161 tttgccacac ggcaggctgc agaagtggct gctgagaagt cctttaataa gttgattgta
     2221 aatggccgca gactgaatgt gaaatgggga agatcccagg cagccagagg aaaagaaaaa
     2281 gagaaagatg gaactacaga ctctgggatc aaactagaac ctgttccagg attgccagga
     2341 gctcttcctc ctcctcctgc agcagaagaa gaagcctctg ccaactactt caacttgccc
     2401 ccaagtggtc ctccagctgt ggtgaacatt gctctgccac cgccccctgg cattgctcca
     2461 cccccacccc caggttttgg gccacacatg ttccacccaa tgggaccacc ccctcctttc
     2521 atgcgggctc caggaccaat ccactatcct tctcaggacc ctcagaggat gggagctcat
     2581 gctggaaaac acagcagccc ctagccatgg tttaaacccg ctgatcagcc tcgactgtgc
     2641 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     2701 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     2761 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     2821 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     2881 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaaat aacttcgtat
     2941 aatgtatgct atacgaagtt atatcgtatg catgctttgc atacttctgc ctgctgggga
     3001 gcctggggac tttccacacc ctaactgaca cacattccac agaattcctt gaaatacatc
     3061 cacaggtaca cctccaattg actcaaatga tgtcaattag tctatcagaa gcttctaaag
     3121 ccatgacatc attttctgga attttccaag ctgtttaaag gcacagtcaa cttagtgtat
     3181 gtaaacttct gacccactgg aattgtgata cagtgaatta taagtgaaat aatctgtctg
     3241 taaacaattg ttggaaaaat gacttgtgtc atgcacaaag tagatgtcct aactgacttg
     3301 ccaaaactat tgtttgttaa caagaaattt gtggagtagt tgaaaaacga gttttaatga
     3361 ctccaactta agtgtatgta aacttccgac ttcaactgta gggtaccgag ctcgaattca
     3421 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     3481 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     3541 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt
     3601 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
     3661 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     3721 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     3781 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     3841 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     3901 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     3961 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     4021 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     4081 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     4141 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     4201 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     4261 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     4321 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     4381 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     4441 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     4501 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     4561 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     4621 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     4681 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     4741 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     4801 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     4861 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     4921 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     4981 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     5041 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     5101 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     5161 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     5221 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     5281 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     5341 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     5401 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     5461 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     5521 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     5581 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     5641 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     5701 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     5761 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     5821 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     5881 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     5941 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     6001 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcttgcatgc
     6061 ctgcaggtcg actctagacc tatacagttg aagtcggaag tttacataca cttaagttgg
     6121 agtcattaaa actcgttttt caactactcc acaaatttct tgttaacaaa caatagtttt
     6181 ggcaagtcag ttaggacatc tactttgtgc atgacacaag tcatttttcc aacaattgtt
     6241 tacagacaga ttatttcact tataattcac tgtatcacaa ttccagtggg tcagaagttt
     6301 acatacacta agttgactgt gcctttaaac agcttggaaa attccagaaa atgatgtcat
     6361 ggctttagaa gcttctgata gactaattga catcatttga gtcaattgga ggtgtacctg
     6421 tggatgtatt tcaaggggat ccagacatga taagatacat tgatgagttt ggacaaacca
     6481 caactagaat gcagcggccg ctagctcgag atcttaatta aactagtatc gatataactt
     6541 cgtataatgt atgctatacg aagttatatg tacgggccag atatacgcgt tgacattgat
     6601 tattgactag gcttttgcaa aaagctttgc aaagatggat aaagttttaa acagagagga
     6661 atctttgcag ctaatggacc ttctaggtct tgaaaggagt gggaatt
//
