LOCUS       V026075                 6664 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V026075
VERSION     V026075
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        90..1268
                     /note="strong constitutive promoter for human elongation
                     factor EF-1α"
                     /label="EF-1α promoter"
     promoter        1285..1303
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     protein_bind    1382..1406
                     /bound_moiety="BP Clonase™"
                     /gene="mutant version of&nbsp;attB"
                     /note="recombination site for the Gateway® BP reaction"
                     /label="attB1"
     CDS             1417..1446
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             1453..2514
                     /label="MBNL3(NM_001386896)"
                     /note="MBNL3(NM_001386896)"
                     /gene="MBNL3"
     protein_bind    complement(2519..2543)
                     /bound_moiety="BP Clonase™"
                     /gene="mutant version of&nbsp;attB"
                     /note="recombination site for the Gateway® BP reaction"
                     /label="attB2"
     CDS             2596..2637
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /transl_table=1
                     /translation="GKPIPNPLLGLDST"
                     /label="V5 tag"
     polyA_signal    2681..2905
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     protein_bind    2975..3008
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
                     /label="loxP"
     primer_bind     complement(3466..3482)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
     promoter        3956..4060
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             4061..4921
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      5092..5680
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     protein_bind    5968..5989
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     promoter        6004..6034
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6042..6058
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     6066..6082
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6580..6613
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
                     /label="loxP"
ORIGIN
        1 aaaaagcttt gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga
       61 ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca
      121 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
      181 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
      241 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
      301 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
      361 cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg
      421 atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc
      481 cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct
      541 ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt
      601 gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc
      661 tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc
      721 gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt
      781 ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct
      841 gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg
      901 gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt
      961 cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg
     1021 agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt
     1081 taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg
     1141 aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg
     1201 gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg
     1261 tgtcgtgagg aattagcttg gtactaatac gactcactat agggagaccc aagctggcta
     1321 ggtaagcttg gtaccgagct cggatccact agtccagtgt ggtggaattc tgcagatatc
     1381 aacaagtttg tacaaaaaag caggcttcgt accatggagc agaagctgat ctccgaggag
     1441 gacctgaagg atatgacggc tgtcaatgtt gccctgattc gtgataccaa gtggctgact
     1501 ttagaagtct gtagagaatt tcagagagga acttgctctc gagctgatgc agattgcaag
     1561 tttgcccatc caccaagagt ttgccatgtg gaaaatggtc gtgtggtggc ctgttttgat
     1621 tctctaaagg gtcggtgtac ccgagagaac tgcaagtacc ttcaccctcc tccacactta
     1681 aaaacgcagc tggagattaa tgggcggaac aatctgattc aacagaagac tgccgcagcc
     1741 atgttcgccc agcagatgca gcttatgctc caaaacgctc aaatgtcatc acttggttct
     1801 tttcctatga ctccatcaat tccagctaat cctcccatgg ctttcaatcc ttacatacca
     1861 catcctggga tgggcctcgt tcctgcagaa cttgtaccaa atacacctgt tctgattcct
     1921 ggaaacccac ctcttgcaat gccaggagct gttggcccaa aactgatgcg ttcagataaa
     1981 ctggaggttt gccgagaatt tcagcgtgga aattgtaccc gtggggagaa tgattgccgc
     2041 tatgctcacc ctactgatgc ttccatgatt gaagcgagtg ataatactgt gacaatctgc
     2101 atggattaca tcaaaggtcg atgctcgcgg gagaaatgca agtactttca tcctcctgca
     2161 cacttgcaag ccagactcaa ggcagctcat catcagatga accattcagc tgcctctgcc
     2221 atggccctgc agcctggtac actgcaactg ataccaaaga gatcagcact ggaaaagccc
     2281 aatggtgcca ccccggtctt taatcccact gttttccact gccaacaggc tctgactaac
     2341 ctgcagctcc cacagccggc atttatccct gcagggccaa tactgtgcat ggcacccgct
     2401 tcaaatattg tgcccatgat gcacggtgct acacctacca ctgtgtctgc agcaacaaca
     2461 cctgccacca gcgttccgtt cgctgcacca actacaggca atcagctgaa attctgagac
     2521 ccagctttct tgtacaaagt ggttgatatc cagcacagtg gcggccgctc gagtctagag
     2581 ggcccgcggt tcgaaggtaa gcctatccct aaccctctcc tcggtctcga ttctacgcgt
     2641 accggttagt aatgagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     2701 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     2761 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     2821 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     2881 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     2941 gggtatcccc acgcgccctg tagcggcgca ttaaataact tcgtataatg tatgctatac
     3001 gaagttatat cgtatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc
     3061 cacaccctaa ctgacacaca ttccacagaa ttccttgaaa tacatccaca ggtacacctc
     3121 caattgactc aaatgatgtc aattagtcta tcagaagctt ctaaagccat gacatcattt
     3181 tctggaattt tccaagctgt ttaaaggcac agtcaactta gtgtatgtaa acttctgacc
     3241 cactggaatt gtgatacagt gaattataag tgaaataatc tgtctgtaaa caattgttgg
     3301 aaaaatgact tgtgtcatgc acaaagtaga tgtcctaact gacttgccaa aactattgtt
     3361 tgttaacaag aaatttgtgg agtagttgaa aaacgagttt taatgactcc aacttaagtg
     3421 tatgtaaact tccgacttca actgtagggt accgagctcg aattcactgg ccgtcgtttt
     3481 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     3541 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     3601 gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     3661 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     3721 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     3781 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     3841 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     3901 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     3961 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     4021 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     4081 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     4141 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     4201 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     4261 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     4321 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     4381 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     4441 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     4501 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     4561 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     4621 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     4681 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     4741 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     4801 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     4861 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     4921 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     4981 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     5041 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     5101 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     5161 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     5221 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     5281 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     5341 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     5401 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     5461 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     5521 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     5581 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     5641 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     5701 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     5761 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     5821 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     5881 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     5941 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     6001 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     6061 tcacacagga aacagctatg accatgatta cgccaagctt gcatgcctgc aggtcgactc
     6121 tagacctata cagttgaagt cggaagttta catacactta agttggagtc attaaaactc
     6181 gtttttcaac tactccacaa atttcttgtt aacaaacaat agttttggca agtcagttag
     6241 gacatctact ttgtgcatga cacaagtcat ttttccaaca attgtttaca gacagattat
     6301 ttcacttata attcactgta tcacaattcc agtgggtcag aagtttacat acactaagtt
     6361 gactgtgcct ttaaacagct tggaaaattc cagaaaatga tgtcatggct ttagaagctt
     6421 ctgatagact aattgacatc atttgagtca attggaggtg tacctgtgga tgtatttcaa
     6481 ggggatccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     6541 cggccgctag ctcgagatct taattaaact agtatcgata taacttcgta taatgtatgc
     6601 tatacgaagt tatatgtacg ggccagatat acgcgttgac attgattatt gactaggctt
     6661 ttgc
//
