LOCUS       40924_42234        7438 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pT2gammaCryK2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7438)
  AUTHORS   Loots G, Bergmann A, Hum N, Hu N, Oldenburg C, Khokha M, Ovcharenko 
            I, Harland RM.
  TITLE     Interrogating Distal Muscle Enhancers in Tol2-mediated Xenopus 
            Transgenics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7438)
  AUTHORS   Loots G, Bergmann A, Hum N.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 7438)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7438)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-AUG-2011) Biosciences "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        156..174
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             1670..1681
                     /codon_start=1
                     /label=WELQut site
                     /note="WELQut protease recognition and cleavage site"
                     /translation="WELQ"
     CDS             2435..3130
                     /codon_start=1
                     /label=mKate2
                     /note="monomeric far-red fluorescent protein (Shcherbo et
                     al., 2009)"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     polyA_signal    3255..3376
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    3388..4039
                     /label=Tol2 transposase recombinant sequence 1
                     /note="Tol2 transposase recombinant sequence 1"
     promoter        complement(4185..4203)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(4461..5049)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5223..6080)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6081..6185)
                     /label=AmpR promoter
     promoter        6531..6549
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     6593..6609
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    7281..7415
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 aattctgcag atatctgcaa agaggggtta actattcttg tgaattattc attctttata
       61 tacatcgcag tttatctaag atgggcacct gcatctacaa agcaaaagtg ttgtacatca
      121 acaaatagca ctctgcaggc aagcttgggg cgcgcaatta accctcacta aagggaacaa
      181 aagctggtac cgggcccccc ctcgagtcca taagaaagat ataaataagc tggagagaat
      241 gcagaattgt caactagcag tgctggaatt ggggagggat gccatcccct cactttttta
      301 tttctgtgaa atagcatccc ctcagtgtgg atgcaagttt tttgaatccc ggaactgtct
      361 tttgctcctc ttcacttctg catgcaaata caatgcagag gtaaaatata atatagttaa
      421 atatataggg gattaaagga gagaaaaaat tgctgggaga gaagaagaaa agatagagaa
      481 gtgttaaaca gataaagaag gtggaggcat cagagagggt tatagagaaa aggagagaag
      541 aaaaaatgaa ggtattaaac agcagcagat taaggaatag tgagaaaatg ctttatatga
      601 aataatcttg ggtaatatag aatatgatgg ggatgtgcta tgttatgaca caatacacag
      661 aaaaggtgct ttatttgcag tagctgaatt ggtagccaag aaaagtcata agcacaattt
      721 ttattttggg gtccctatag gtcacatatg taaatatggt tatgggaggg atgcaccaca
      781 cagagcatcc ctccactttt tttactccaa ttcaagcact gggaatgaga tatgcttcat
      841 tagcacctaa acatttgaca atgaccaatg tgaatgttag cgaccatatt tgcgtccttt
      901 tgtgaccaaa tggcatatgt gaacattcct agtgtatgta ataaaatttt gcaacaaaat
      961 atttaaaaac tccagagcag atgcttaact tagtcatgta atattcgcca gcaaatgagt
     1021 atacctctat ttttaacatc acttgtgatt tttttagtaa cattcccacc tttagtattt
     1081 ccagattgaa attatcatac ccccccattc tttaatatag ggaacactgt accagtgggt
     1141 ctgggattat ttgtccatat attgcaatat atccaagctg accaactgtc tcaaagtcac
     1201 ccctggtctg gccaataaaa cacaaacatt ctagcagaaa gtaaagctgg ccatagatgt
     1261 tgagattttt aaaagatcag atcctgatcg tgagaccacg atcttctcag aacgatcgta
     1321 cgatcgtacg aatctaccat caactaaaaa gaccaattta ccaggaaaac aaaggggagc
     1381 tgcctgcttg gccctgcaaa catagagaga ttgcactggg gccgacaaag attttttgac
     1441 ctggccgatc aatttcccga cagatgtcgg ccgaaaaatc gtaagatgta cgatcgttcg
     1501 aataccacta accgcacgat aatttcgaag gattggtcgg gcttccctaa aatcggtcgt
     1561 tcggcaagaa gaatcgtcgc gtctatgggg accttaagtg tacttgcttt ccaaggttaa
     1621 aaattgttac ctttttatac gacactgcat ggatcacctg aaagcaggtt gggagctaca
     1681 acatggaact agccactgtt cctttatatg gttatcacta cccaatttta aataattaaa
     1741 ctggagttta ttgatggtgt gaccacaaaa ttgatacaga taaagacata ctgtatatat
     1801 tgcgttatat gacaaataca gtaacaccac attactattt taaattatat ataaaataat
     1861 attaatatat ctggggacat aaaatgtggc ttcttaaaca ttggggtgac actgatgaga
     1921 ttatttggta aatgtttgat gctagctaac cattgtttgg ttcatttgtg tgattcaaaa
     1981 gtggcaaaga atgacaccta gatgatgcca aattaaggat ttactagtaa ccacaattct
     2041 ttaaactgtt acaaagtgtt tggaccagca actgaattgc actgtaaatt accacatcag
     2101 ggtctccggg gtcaacgctg ctaagcaatg gactggaaaa tttgtgacat tgagtttttg
     2161 gaataactgg gaatgctgga taactctttg ggtaaagcta tggaacaaaa gtaccagcat
     2221 gaactaagaa atgactgtca cagcacaaca tcaaacacaa tgcaagacaa aggttctctg
     2281 cttttgcctt acttgacaga aaaagtaagc tctaggttca tcgcatgcag acagcagcaa
     2341 tacatgacat atataaaggc tggctctttt ctcccttcca ttgaactgaa acttccactc
     2401 agtcagactt gcgggggatc caccggtcgc caccatggtg agcgagctga ttaaggagaa
     2461 catgcacatg aagctgtaca tggagggcac cgtgaacaac caccacttca agtgcacatc
     2521 cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg cggtcgaggg
     2581 cggccctctc cccttcgcct tcgacatcct ggctaccagc ttcatgtacg gcagcaaaac
     2641 cttcatcaac cacacccagg gcatccccga cttctttaag cagtccttcc ccgagggctt
     2701 cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta cccaggacac
     2761 cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga acttcccatc
     2821 caacggccct gtgatgcaga agaaaacact cggctgggag gcctccaccg agaccctgta
     2881 ccccgctgac ggcggcctgg aaggcagagc cgacatggcc ctgaagctcg tgggcggggg
     2941 ccacctgatc tgcaacttga agaccacata cagatccaag aaacccgcta agaacctcaa
     3001 gatgcccggc gtctactatg tggacagaag actggaaaga atcaaggagg ccgacaaaga
     3061 gacctacgtc gagcagcacg aggtggctgt ggccagatac tgcgacctcc ctagcaaact
     3121 ggggcacaga tgagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg
     3181 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     3241 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     3301 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     3361 tcatcaatgt atcttaatcg agggcccatc tggcctgtgt ttcagacacc agggagtctc
     3421 tgctcacgtt tcctgctatt tgcagcctct ctatcaagac taatacacct cttcccgcat
     3481 cggctgcctg tgagaggctt ttcagcactg caggattgct tttcagcccc aaaagagcta
     3541 ggcttgacac taacaatttt gagaatcagc ttctactgaa gttaaatctg aggttttaca
     3601 actttgagta gcgtgtactg gcattagatt gtctgtctta tagtttgata attaaataca
     3661 aacagttcta aagcaggata aaaccttgta tgcatttcat ttaatgtttt ttgagattaa
     3721 aagcttaaac aagaatctct agttttcttt cttgctttta cttttacttc cttaatactc
     3781 aagtacaatt ttaatggagt acttttttac ttttactcaa gtaagattct agccagatac
     3841 ttttactttt aattgagtaa aattttccct aagtacttgt actttcactt gagtaaaatt
     3901 tttgagtact ttttacacct ctgtcaagaa ctcctggaca aacctctgac ctgtgtggaa
     3961 cagagtggat atgggtgtct gaacagatat tcacgtcttt tgcagatcag agggcatttc
     4021 tggtgttgtg caatgacctg ggtccaacag gtaggcgtac tcgtatccaa gagcattgtt
     4081 ggacaggagg tagtctccat tcctataaag aggaaggaat ggcaccatgt agtagccgtc
     4141 attgtggcca attggtgcat tggtacatca gatctgccgg tctccctata gtgagtcgta
     4201 ttaatttcga taagccaggt taacctgcat taatgaatcg gccaacgcgc ggggagaggc
     4261 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     4321 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     4381 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     4441 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     4501 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     4561 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     4621 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     4681 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     4741 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     4801 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     4861 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     4921 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     4981 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     5041 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     5101 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     5161 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     5221 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     5281 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     5341 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     5401 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     5461 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     5521 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     5581 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     5641 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     5701 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     5761 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     5821 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     5881 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     5941 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     6001 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     6061 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     6121 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     6181 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     6241 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
     6301 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     6361 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
     6421 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgga catattgtcg
     6481 ttagaacgcg gctacaatta atacataacc ttatgtatca tacacatacg atttaggtga
     6541 cactatagaa ctcgagcagc tgaagcttgc atgcctgcag gtcgactcta gctctagaac
     6601 tagtggatct gctgggcttg ctgaaggtag ggggtcaaga accagaggtg taaagtactt
     6661 gagtaatttt acttgattac tgtacttaag tattattttt ggggattttt actttacttg
     6721 agtacaatta aaaatcaata cttttacttt tacttaatta cattttttta gaaaaaaaag
     6781 tactttttac tccttacaat tttatttaca gtcaaaaagt acttattttt tggagatcac
     6841 ttcattctat tttcccttgc tattaccaaa ccaattgaat tgcgctgatg cccagtttaa
     6901 tttaaatgtt atttattctg cctatgaaaa tcgttttcac attatatgaa attggtcaga
     6961 catgttcatt ggtcctttgg aagtgacgtc atgtcacatc tattaccaca atgcacagca
     7021 ccttgacctg gaaattaggg aaattataac agtcaatcag tggaagaaaa tggaggaagt
     7081 atgtgattca tcagcagctg cgagcagcac agtccaaaat cagccacagg atcaagagca
     7141 cccgtggccg tatcttcgca gatctgatct agaggatcat aatcagccat accacatttg
     7201 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     7261 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     7321 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     7381 ccaaactcat caatgtatct tatcatgtct ggatcatcat cgtcgagctc aagcttcg
//
