LOCUS       62056_20470       13457 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13457)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..13457
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        75..93
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    413..446
                     /label=lox2272
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     intron          1136..2144
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     CDS             2208..3263
                     /codon_start=1
                     /label=mPou5f1
                     /note="Mus musculus Oct-4 gene. Encodes a transcription
                     factor containing a POU homeodomain that plays a key role 
                     in embryonic development and stem cell pluripotency."
                     /translation="MAGHLASDFAFSPPPGGGDGSAGLEPGWVDPRTWLSFQGPPGGPG
                     IGPGSEVLGISPCPPAYEFCGGMAYCGPQVGLGLVPQVGVETLQPEGQAGARVESNSEG
                     TSSEPCADRPNAVKLEKVEPTPEESQDMKALQKELEQFAKLLKQKRITLGYTQADVGLT
                     LGVLFGKVFSQTTICRFEALQLSLKNMCKLRPLLEKWVEEADNNENLQEICKSETLVQA
                     RKRKRTSIENRVRWSLETMFLKCPKPSLQQITHIANQLGLEKDVVRVWFCNRRQKGKRS
                     SIEYSQREEYEATGTPFPGGAVSFPLPPGPHFGTPGYGSPHFTTLYSVPFPEGEAFPSV
                     PVTALGSPMHSN"
     CDS             3336..4292
                     /codon_start=1
                     /label=mSox2
                     /note="Mus musculus transcription factor SOX-2 gene.
                     Belongs to the SRY-related HMG-box (SOX) family of 
                     transcription factors, which is involved in the regulation 
                     of embryonic development and in the determination of cell 
                     fate"
                     /translation="MYNMMETELKPPGPQQASGGGGGGGNATAAATGGNQKNSPDRVKR
                     PMNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLLSETEKRPFIDEAKRLRALHM
                     KEHPDYKYRPRRKTKTLMKKDKYTLPGGLLAPGGNSMASGVGVGAGLGAGVNQRMDSYA
                     HMNGWSNGSYSMMQEQLGYPQHPGLNAHGAAQMQPMHRYDVSALQYNSMTSSQTYMNGS
                     PTYSMSYSQQGTPGMALGSMGSVVKSEASSSPPVVTSSSHSRAPCQAGDLRDMISMYLP
                     GAEVPEPAAPSRLHMAQHYQSGPVPGTAINGTLPLSHM"
     CDS             4365..5786
                     /codon_start=1
                     /label=mKlf4
                     /note="Mouse Kruppel-like factor (Klf4) gene. Belongs to
                     the relatively large family of SP1-like transcription 
                     factors and is involved in the regulation of proliferation,
                     differentiation, apoptosis and somatic cell reprogramming."
                     /translation="MAVSDALLPSFSTFASGPAGREKTLRPAGAPTNRWREELSHMKRL
                     PPLPGRPYDLAATVATDLESGGAGAACSSNNPALLARRETEEFNDLLDLDFILSNSLTH
                     QESVAATVTTSASASSSSSPASSGPASAPSTCSFSYPIRAGGDPGVAARNTGGGLLYSR
                     ESAPPPTAPFNLGDINDVSPSGGFVAELLRPELDPVYIPPQQPQPPGGGLMGKFVLKAS
                     LTTPGSEYSSPSVISVSKGSPDGSHPVVVAPYSGGPPRMCPKIKQEAVPSCTVSRSLEA
                     HLSAGPQLSNGHRPNTHDFPLGRQLPTRTTPTLSPEELLNSRDCHPGLPLPPGFHPHPG
                     ANYPPFLPDQMQSQVPSLHYQELMPPGSCLPEEPKPKRGRRSWPRKRTATHTCDYAGCG
                     KTYTKSSHLKAHLRTHTGEKPYHCDWDGCGWKFARSDELTRHYRKHTGHRPFQCQKCDR
                     AFSRSDHLALHMKRHF"
     CDS             5865..7181
                     /codon_start=1
                     /label=mMyc
                     /note="Mus musculus myc proto-oncogene. Belongs to 
                     myelocytomatosis (Myc) family of transcription factors. 
                     Plays a role in cell cycle procession, apotosis and 
                     cellular transformation"
                     /translation="MPLNVNFTNRNYDLDYDSVQPYFICDEEENFYHQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVATSFSPREDDDGGGGNFSTADQLEMMT
                     ELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSTSL
                     SPARGHSVCSTSSLYLQDLTAAASECIDPSVVFPYPLNDSSSPKSCTSSDSTAFSPSSD
                     SLLSSESSPRASPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQTPAKRSESGS
                     SPSRGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRAKLDSGRVLKQISNNR
                     KCSSPRSSDTEENDKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSIQADEHKLTSEKDLLRKRREQLKHKLEQLRNSGA"
     polyA_signal    7228..7452
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    7752..7785
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    7798..7845
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        7871..8370
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             8396..8989
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     CDS             8996..9988
                     /codon_start=1
                     /label=Delta-TK
                     /note="truncated herpes simplex virus thymidine kinase
                     (Salomon et al., 1995)"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     polyA_signal    10019..10243
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(10320..10353)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(10718..10736)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(10805..10835)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(10850..10871)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(11159..11747)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11921..12778)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12779..12883)
                     /label=AmpR promoter
     rep_origin      12910..13365
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
       61 ccagtgagcg cgcgtaatac gactcactat agggcgaatt ggagctcgga tccctataca
      121 gttgaagtcg gaagtttaca tacacttaag ttggagtcat taaaactcgt ttttcaacta
      181 ctccacaaat ttcttgttaa caaacaatag ttttggcaag tcagttagga catctacttt
      241 gtgcatgaca caagtcattt ttccaacaat tgtttacaga cagattattt cacttataat
      301 tcactgtatc acaattccag tgggtcagaa gtttacatac actaagttga ctgtgccttt
      361 aaacagcttg gaaaattcca gaaaatgatg tcatggcttt agaagcttcg agataacttc
      421 gtataaagtc tcctatacga agttatgatg ggggatccac tagcatgggc ccatctcgac
      481 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      541 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      601 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      661 tccattgacg tcaatgggtg gactatttac ggtaaactgc ccacttggca gtacatcaag
      721 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      781 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      841 tcatcgctat taccatgggt cgaggtgagc cccacgttct gcttcactct ccccatctcc
      901 cccccctccc cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg
      961 ggggcggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg
     1021 ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt
     1081 tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt
     1141 cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc cgccccggct
     1201 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg
     1261 taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg aaagccttaa
     1321 agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg tgcgtgtgtg
     1381 tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc gctgcgggcg
     1441 cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg gggcggtgcc
     1501 ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg
     1561 ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca cccccctccc
     1621 cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtgcggggcg tggcgcgggg
     1681 ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct
     1741 cgggccgggg agggctcggg ggaggggcgc ggcggccccg gagcgccggc ggctgtcgag
     1801 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
     1861 ctttgtccca aatctggcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg
     1921 gcgcgggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg
     1981 tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgccgcag ggggacggct
     2041 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
     2101 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
     2161 tggttgttgt gctgtctcat cattttggca aagaattcag atccaccatg gctggacacc
     2221 tggcttcaga cttcgccttc tcacccccac caggtggggg tgatgggtca gcagggctgg
     2281 agccgggctg ggtggatcct cgaacctggc taagcttcca agggcctcca ggtgggcctg
     2341 gaatcggacc aggctcagag gtattgggga tctccccatg tccgcccgca tacgagttct
     2401 gcggagggat ggcatactgt ggacctcagg ttggactggg cctagtcccc caagttggcg
     2461 tggagacttt gcagcctgag ggccaggcag gagcacgagt ggaaagcaac tcagagggaa
     2521 cctcctctga gccctgtgcc gaccgcccca atgccgtgaa gttggagaag gtggaaccaa
     2581 ctcccgagga gtcccaggac atgaaagccc tgcagaagga gctagaacag tttgccaagc
     2641 tgctgaagca gaagaggatc accttggggt acacccaggc cgacgtgggg ctcaccctgg
     2701 gcgttctctt tggaaaggtg ttcagccaga ccaccatctg tcgcttcgag gccttgcagc
     2761 tcagccttaa gaacatgtgt aagctgcggc ccctgctgga gaagtgggtg gaggaagccg
     2821 acaacaatga gaaccttcag gagatatgca aatcggagac cctggtgcag gcccggaaga
     2881 gaaagcgaac tagcattgag aaccgtgtga ggtggagtct ggagaccatg tttctgaagt
     2941 gcccgaagcc ctccctacag cagatcactc acatcgccaa tcagcttggg ctagagaagg
     3001 atgtggttcg agtatggttc tgtaaccggc gccagaaggg caaaagatca agtattgagt
     3061 attcccaacg agaagagtat gaggctacag ggacaccttt cccagggggg gctgtatcct
     3121 ttcctctgcc cccaggtccc cactttggca ccccaggcta tggaagcccc cacttcacca
     3181 cactctactc agtccctttt cctgagggcg aggcctttcc ctctgttccc gtcactgctc
     3241 tgggctctcc catgcattca aacggcggcg ggtccggagg agagggcaga ggaagtcttc
     3301 taacatgcgg tgacgtggag gagaatcctg gcccaatgta taacatgatg gagacggagc
     3361 tgaagccgcc gggcccgcag caagcttcgg ggggcggcgg cggaggaggc aacgccacgg
     3421 cggcggcgac cggcggcaac cagaagaaca gcccggaccg cgtcaagagg cccatgaacg
     3481 ccttcatggt atggtcccgg gggcagcggc gtaagatggc ccaggagaac cccaagatgc
     3541 acaactcgga gatcagcaag cgcctgggcg cggagtggaa acttttgtcc gagaccgaga
     3601 agcggccgtt catcgacgag gccaagcggc tgcgcgctct gcacatgaag gagcacccgg
     3661 attataaata ccggccgcgg cggaaaacca agacgctcat gaagaaggat aagtacacgc
     3721 ttcccggagg cttgctggcc cccggcggga acagcatggc gagcggggtt ggggtgggcg
     3781 ccggcctggg tgcgggcgtg aaccagcgca tggacagcta cgcgcacatg aacggctgga
     3841 gcaacggcag ctacagcatg atgcaggagc agctgggcta cccgcagcac ccgggcctca
     3901 acgctcacgg cgcggcacag atgcaaccga tgcaccgcta cgacgtcagc gccctgcagt
     3961 acaactccat gaccagctcg cagacctaca tgaacggctc gcccacctac agcatgtcct
     4021 actcgcagca gggcaccccc ggtatggcgc tgggctccat gggctctgtg gtcaagtccg
     4081 aggccagctc cagccccccc gtggttacct cttcctccca ctccagggcg ccctgccagg
     4141 ccggggacct ccgggacatg atcagcatgt acctccccgg cgccgaggtg ccggagcccg
     4201 ctgcgcccag tagactgcac atggcccagc actaccagag cggcccggtg cccggcacgg
     4261 ccattaacgg cacactgccc ctgtcgcaca tgggcggcgg gtccggagga gagggcagag
     4321 gaagtcttct aacatgcggt gacgtggagg agaatcctgg cccaatggct gtcagcgacg
     4381 ctctgctccc gtccttctcc acgttcgcgt ccggcccggc gggaagggag aagacactgc
     4441 gtccagcagg tgccccgact aaccgttggc gtgaggaact ctctcacatg aagcgacttc
     4501 ccccacttcc cggccgcccc tacgacctgg cggcgacggt ggccacagac ctggagagtg
     4561 gcggagctgg tgcagcttgc agcagtaaca acccggccct cctagcccgg agggagaccg
     4621 aggagttcaa cgacctcctg gacctagact ttatcctttc caactcgcta acccaccagg
     4681 aatcggtggc cgccaccgtg accacctcgg cgtcagcttc atcctcgtct tccccggcga
     4741 gcagcggccc tgccagcgcg ccctccacct gcagcttcag ctatccgatc cgggccgggg
     4801 gtgacccggg cgtggctgcc agaaacacag gtggagggct cctctacagc cgagaatctg
     4861 cgccacctcc cacggccccc ttcaacctgg gggacatcaa tgacgtgagc ccctcgggcg
     4921 gcttcgtggc tgagctcctg cggccggagt tggacccagt atacattccg ccacagcagc
     4981 ctcagccgcc aggtgggggg ctgatgggca agtttgtgct gaaggcgtct ctgaccaccc
     5041 ctggcagcga gtacagcagc ccttcggtca tcagtgttag caaaggaagc ccagacggca
     5101 gccaccccgt ggtagtggcg ccctacagcg gtggcccgcc gcgcatgtgc cccaagatta
     5161 agcaagaggc ggtcccgtcc tgcacggtca gccggtccct agaggcccat ttgagcgctg
     5221 gaccccagct cagcaacggc caccggccca acacacacga cttccccctg gggcggcagc
     5281 tccccaccag gactacccct acactgagtc ccgaggaact gctgaacagc agggactgtc
     5341 accctggcct gcctcttccc ccaggattcc atccccatcc gggggccaac taccctcctt
     5401 tcctgccaga ccagatgcag tcacaagtcc cctctctcca ttatcaagag ctcatgccac
     5461 cgggttcctg cctgccagag gagcccaagc caaagagggg aagaaggtcg tggccccgga
     5521 aaagaacagc cacccacact tgtgactatg caggctgtgg caaaacctat accaagagtt
     5581 ctcatctcaa ggcacacctg cgaactcaca caggcgagaa accttaccac tgtgactggg
     5641 acggctgtgg gtggaaattc gcccgctccg atgaactgac caggcactac cgcaaacaca
     5701 cagggcaccg gccctttcag tgccagaagt gtgacagggc cttttccagg tcggaccacc
     5761 ttgccttaca catgaagagg cactttggca gcggaaaaca gctgttgaat tttgaccttc
     5821 tcaagttggc gggagacgtg gagtccaacc cagggcccgt cgagatgccc ctcaacgtga
     5881 acttcaccaa caggaactat gacctcgact acgactccgt acagccctat ttcatctgcg
     5941 acgaggaaga gaatttctat caccagcaac agcagagcga gctgcagccg cccgcgccca
     6001 gtgaggatat ctggaagaaa ttcgagctgc ttcccacccc gcccctgtcc ccgagccgcc
     6061 gctccgggct ctgctctcca tcctatgttg cggtcgctac gtccttctcc ccaagggaag
     6121 acgatgacgg cggcggtggc aacttctcca ccgccgatca gctggagatg atgaccgagt
     6181 tacttggagg agacatggtg aaccagagct tcatctgcga tcctgacgac gagaccttca
     6241 tcaagaacat catcatccag gactgtatgt ggagcggttt ctcagccgct gccaagctgg
     6301 tctcggagaa gctggcctcc taccaggctg cgcgcaaaga cagcaccagc ctgagccccg
     6361 cccgcgggca cagcgtctgc tccacctcca gcctgtacct gcaggacctc accgccgccg
     6421 cgtccgagtg cattgacccc tcagtggtct ttccctaccc gctcaacgac agcagctcgc
     6481 ccaaatcctg tacctcgtcc gattccacgg ccttctctcc ttcctcggac tcgctgctgt
     6541 cctccgagtc ctccccacgg gccagccctg agcccctagt gctgcatgag gagacaccgc
     6601 ccaccaccag cagcgactct gaagaagagc aagaagatga ggaagaaatt gatgtggtgt
     6661 ctgtggagaa gaggcaaacc cctgccaaga ggtcggagtc gggctcatct ccatcccgag
     6721 gccacagcaa acctccgcac agcccactgg tcctcaagag gtgccacgtc tccactcacc
     6781 agcacaacta cgccgcaccc ccctccacaa ggaaggacta tccagctgcc aagagggcca
     6841 agttggacag tggcagggtc ctgaagcaga tcagcaacaa ccgcaagtgc tccagcccca
     6901 ggtcctcaga cacggaggaa aacgacaaga ggcggacaca caacgtcttg gaacgtcaga
     6961 ggaggaacga gctgaagcgc agcttttttg ccctgcgtga ccagatccct gaattggaaa
     7021 acaacgaaaa ggcccccaag gtagtgatcc tcaaaaaagc caccgcctac atcctgtcca
     7081 ttcaagcaga cgagcacaag ctcacctctg aaaaggactt attgaggaaa cgacgagaac
     7141 agttgaaaca caaactcgaa cagcttcgaa actctggtgc atgagtcgag atgcatcgat
     7201 gatctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     7261 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     7321 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     7381 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     7441 tgggctctat ggcttctgag gcggaaagaa cctgcagccc aagcttggcg taatcatggt
     7501 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     7561 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     7621 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc cattcatgaa
     7681 tgaattcatg tcgaggtcga gacggtatcg atggcgcgga attcaaaaag tttaaacaaa
     7741 aaaggcgcgc cataacttcg tataatgtat gctatacgaa gttataagct taattaagaa
     7801 gttcctattc cgaagttcct attctctaga aagtatagga acttcagctt gatatcgaat
     7861 taattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
     7921 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
     7981 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
     8041 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
     8101 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
     8161 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
     8221 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc
     8281 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
     8341 ttctcctctt cctcatctcc gggcctttcg acctgcagcc aacgccacca tggggaccga
     8401 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct
     8461 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat
     8521 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     8581 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     8641 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     8701 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     8761 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     8821 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     8881 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     8941 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgccg gatccatgcc
     9001 cacgctactg cgggtttata tagacggtcc tcacgggatg gggaaaacca ccaccacgca
     9061 actgctggtg gccctgggtt cgcgcgacga tatcgtctac gtacccgagc cgatgactta
     9121 ctggcaggtg ctgggggctt ccgagacaat cgcgaacatc tacaccacac aacaccgcct
     9181 cgaccagggt gagatatcgg ccggggacgc ggcggtggta atgacaagcg cccagataac
     9241 aatgggcatg ccttatgccg tgaccgacgc cgttctggct cctcatatcg ggggggaggc
     9301 tgggagctca catgccccgc ccccggccct caccctcatc ttcgaccgcc atcccatcgc
     9361 cgccctcctg tgctacccgg ccgcgcgata ccttatgggc agcatgaccc cccaggccgt
     9421 gctggcgttc gtggccctca tcccgccgac cttgcccggc acaaacatcg tgttgggggc
     9481 ccttccggag gacagacaca tcgaccgcct ggccaaacgc cagcgccccg gcgagcggct
     9541 tgacctggct atgctggccg cgattcgccg cgtttacggg ctgcttgcca atacggtgcg
     9601 gtatctgcag ggcggcgggt cgtggcggga ggattgggga cagctttcgg ggacggccgt
     9661 gccgccccag ggtgccgagc cccagagcaa cgcgggccca cgaccccata tcggggacac
     9721 gttatttacc ctgtttcggg cccccgagtt gctggccccc aacggcgacc tgtacaacgt
     9781 gtttgcctgg gccttggacg tcttggccaa acgcctccgt cccatgcacg tctttatcct
     9841 ggattacgac caatcgcccg ccggctgccg ggacgccctg ctgcaactta cctccgggat
     9901 ggtccagacc cacgtcacca cccccggctc cataccgacg atctgcgacc tggcgcgcac
     9961 gtttgcccgg gagatggggg aggctaactg agctctagag ctcgctgatc agcctcgact
    10021 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
    10081 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
    10141 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
    10201 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tgggcggccg ccaccgcggt
    10261 ggagctccag cttttgttcc ctttagtgag ggttaattag atccgataag cttgatatca
    10321 taacttcgta tagcatacat tatacgaagt tatctcgaag cttgtggaag gctactcgaa
    10381 atgtttgacc caagttaaac aatttaaagg caatgctacc aaatactaat tgagtgtatg
    10441 taaacttctg acccactggg aatgtgatga aagaaataaa agctgaaatg aatcattctc
    10501 tctactatta ttctgatatt tcacattctt aaaataaagt ggtgatccta actgacctaa
    10561 gacagggaat ttttactagg attaaatgtc aggaattgtg aaaaagtgag tttaaatgta
    10621 tttggctaag gtgtatgtaa acttccgact tcaactgtat agggatcctc tagctagagt
    10681 cgacctcgag ggggggcccg gtacccagct tttgttccct ttagtgaggg ttaatttcga
    10741 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
    10801 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
    10861 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
    10921 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
    10981 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
    11041 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
    11101 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
    11161 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
    11221 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
    11281 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
    11341 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
    11401 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    11461 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    11521 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    11581 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
    11641 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    11701 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    11761 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    11821 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    11881 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    11941 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    12001 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    12061 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    12121 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    12181 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    12241 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    12301 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    12361 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    12421 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    12481 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    12541 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    12601 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    12661 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    12721 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    12781 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    12841 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    12901 tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
    12961 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
    13021 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
    13081 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
    13141 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
    13201 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
    13261 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
    13321 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttccatt cgccattcag
    13381 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
    13441 gaaaggggga tgtgctg
//
