LOCUS       40924_42129        3554 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pT19mob2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Kirchner O, Tauch A.
  TITLE     Tools for genetic engineering in the amino acid-producing bacterium 
            Corynebacterium glutamicum
  JOURNAL   J. Biotechnol. 104 (1-3), 287-299 (2003)
  PUBMED    12948646
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Kirchner O, Tauch A.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2003) Department of Genetics, University of 
            Bielefeld, Universitaetsstrasse 25, Bielefeld D-33615, Germany
REFERENCE   3  (bases 1 to 3554)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3554)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Biotechnol. 104 (1-3), 287-299 (2003)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-JAN-2003) Department of Genetics, University of Bielefeld, 
            Universitaetsstrasse 25, Bielefeld D-33615, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3554
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(1..57)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(58..74)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1018..1029)
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     oriT            complement(2233..2340)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      2552..3140
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3428..3449
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3464..3494
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3502..3518
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3526..3542
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct cgaattcact
       61 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
      121 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
      181 ttcccaacag ttgcgcagcc tgaatggcga atggcgcgat aagctagctt cacgctgccg
      241 caagcactca gggcgcaagg gctgctaaag gaagcggaac acgtagaaag ccagtccgca
      301 gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc
      361 aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc
      421 ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg
      481 gaagccctgc aaagtaaact ggatggcttt cttgccgcca aggatctgat ggcgcagggg
      541 atcaagatcc tccttcgtcg gtgtcgtcgc cggatggtct gcggtggtgc tcagcgtgga
      601 gacgcgcacc gtcacggacc gcgtgtagtg cgtggcggaa acttcttgcg tttcgcaaga
      661 gaaatgcgtc ccatttctcg tcggactcgg ggaaggaagc gtgatgctct cggtcaagca
      721 cgtcgctcgc cagcgctgcg aggagttcgg ccttcgtgcg gaagtgccag tagaggccgg
      781 gctgctgtac ctgtaagtga gccgccagcg cgcgagtggt gaagccatcg agcccagtct
      841 cgtcgagcac ctgccgggcc ccgagcaaca cggacgtgcg gtcgagacgc ttccggtggt
      901 gagtcatagt tgcactttat catcgataac tttatcttag ataaagtgac tgctcgctac
      961 tctcatctga ctgctcgcta ctctcatcgt ggaatcctga cagccgtgct catcacggcg
     1021 accctcgatg ctgcagggct gggcctcgtg atgccgatct tgcctaccct tctcgaccag
     1081 gtcggtgccc ccgacgacat gatcccactg cacgtcggac tactgacagc gctctatgcg
     1141 atcatgcagt ttctttgcgc cccgatcctt ggccgactct ctgaccgttt cggacgccgc
     1201 cgcgtgcttg tcgcctccct cgcaggcgcg acgatcgact acctcgtgct cgcactgacg
     1261 gacacgctgt gggtctttta cctcgcccgc gcggttgcag gcattaccgg cgccacgaac
     1321 gccgtcaccg cgacggtgat cgccgacatt actccgccgg atcagcgcgc aaaacgctac
     1381 gggtggctcg gcgcatgcta cggcggtggc atgatcgcgg gtcccgccat tggcggtctt
     1441 ttcggcgggg tctcaccgca tctgccattc ctcgtcgccg ccgcgctcgc cggaatcacc
     1501 ctcgtactca gcgcgagtct tctgcgtgag acgcggccac cgggcagcaa cggctcgcac
     1561 gcacagcaac ccggtacggc gaagcgaacc gcagtgccgg ggatgcttat ccttctcgca
     1621 gtcttcggca tcgtgcagtt catcggccaa gcaccaggct ccacctgggt gctcttcacg
     1681 cagcagcgcc tcgactggaa ccccgtcgaa gtcggcgttt cgctatccat cttcggaatg
     1741 gtgcaagtat tcgtgcaggc ggcactgacc ggacgcatcg tgtcccggat cggcgagacc
     1801 cgggcgatcc tcgtcggtat cgccgcagac gccattgggc tcatcggcct tgccctcatc
     1861 gccagcacat gggcgatgct accgatcctc gcagcgctcg gactcggcag catcacgttg
     1921 cccgcactgc agacgctgct ctcgagacgc gcgcccgagc agcagcaggg acgcctgcag
     1981 ggaacacttg caagcctgaa cagcctcacc tcgatcatcg gcccggtcac cttcaccggc
     2041 attttcgcac tcacccgaac gaatgcagac ggcaccctct ggatctgcgc cgcagcgctc
     2101 tacgttctct gcgccctcct gatgatccgt gagacatgcg cctcacggcg atctcgataa
     2161 ccgcgctaag gtgccatccc gatgcgacgg gatcgctctg ccaccagtca agtctcccgt
     2221 agccggtatg agggccagcc tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa
     2281 taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc tatcctgccc
     2341 cgctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca aaatcctgta
     2401 tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc gaagcagggt
     2461 tatgcagcgg aaaagccatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     2521 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     2581 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     2641 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     2701 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     2761 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     2821 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     2881 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     2941 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3001 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3061 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3121 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3181 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3241 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3301 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     3361 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     3421 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     3481 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     3541 accatgatta cgcc
//
