LOCUS       40924_42124        3728 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pT19MECA2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Kirchner O, Tauch A.
  TITLE     Tools for genetic engineering in the amino acid-producing bacterium 
            Corynebacterium glutamicum
  JOURNAL   J. Biotechnol. 104 (1-3), 287-299 (2003)
  PUBMED    12948646
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Kirchner O, Tauch A.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JAN-2003) Department of Genetics, University of 
            Bielefeld, Universitaetsstrasse 25, Bielefeld D-33615, Germany
REFERENCE   3  (bases 1 to 3728)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3728)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Biotechnol. 104 (1-3), 287-299 (2003)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-JAN-2003) Department of Genetics, University of Bielefeld, 
            Universitaetsstrasse 25, Bielefeld D-33615, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3728
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(206..223)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(232..248)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1192..1203)
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     oriT            complement(2407..2514)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      2726..3314
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3602..3623
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3638..3668
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3676..3692
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3700..3716
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagctttaat acgactcact atagggagat gttaaccgcg gtccctaggc cggccgctag
       61 ccatttaaat gcatgctgca gcccgggcga cgtcgactgt ttaaacgagg ccttaattaa
      121 gggcccaatt ggccacgtac gtctagatct ggcgcgcctc gaggatccat ggtaccggtg
      181 cggccgcact agtgatcgat atctcccttt agtgagggtt aatgtgaatt cactggccgt
      241 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
      301 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
      361 acagttgcgc agcctgaatg gcgaatggcg cgataagcta gcttcacgct gccgcaagca
      421 ctcagggcgc aagggctgct aaaggaagcg gaacacgtag aaagccagtc cgcagaaacg
      481 gtgctgaccc cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc
      541 aaagagaaag caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt
      601 atggacagca agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc
      661 ctgcaaagta aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag
      721 atcctccttc gtcggtgtcg tcgccggatg gtctgcggtg gtgctcagcg tggagacgcg
      781 caccgtcacg gaccgcgtgt agtgcgtggc ggaaacttct tgcgtttcgc aagagaaatg
      841 cgtcccattt ctcgtcggac tcggggaagg aagcgtgatg ctctcggtca agcacgtcgc
      901 tcgccagcgc tgcgaggagt tcggccttcg tgcggaagtg ccagtagagg ccgggctgct
      961 gtacctgtaa gtgagccgcc agcgcgcgag tggtgaagcc atcgagccca gtctcgtcga
     1021 gcacctgccg ggccccgagc aacacggacg tgcggtcgag acgcttccgg tggtgagtca
     1081 tagttgcact ttatcatcga taactttatc ttagataaag tgactgctcg ctactctcat
     1141 ctgactgctc gctactctca tcgtggaatc ctgacagccg tgctcatcac ggcgaccctc
     1201 gatgctgcag ggctgggcct cgtgatgccg atcttgccta cccttctcga ccaggtcggt
     1261 gcccccgacg acatgatccc actgcacgtc ggactactga cagcgctcta tgcgatcatg
     1321 cagtttcttt gcgccccgat ccttggccga ctctctgacc gtttcggacg ccgccgcgtg
     1381 cttgtcgcct ccctcgcagg cgcgacgatc gactacctcg tgctcgcact gacggacacg
     1441 ctgtgggtct tttacctcgc ccgcgcggtt gcaggcatta ccggcgccac gaacgccgtc
     1501 accgcgacgg tgatcgccga cattactccg ccggatcagc gcgcaaaacg ctacgggtgg
     1561 ctcggcgcat gctacggcgg tggcatgatc gcgggtcccg ccattggcgg tcttttcggc
     1621 ggggtctcac cgcatctgcc attcctcgtc gccgccgcgc tcgccggaat caccctcgta
     1681 ctcagcgcga gtcttctgcg tgagacgcgg ccaccgggca gcaacggctc gcacgcacag
     1741 caacccggta cggcgaagcg aaccgcagtg ccggggatgc ttatccttct cgcagtcttc
     1801 ggcatcgtgc agttcatcgg ccaagcacca ggctccacct gggtgctctt cacgcagcag
     1861 cgcctcgact ggaaccccgt cgaagtcggc gtttcgctat ccatcttcgg aatggtgcaa
     1921 gtattcgtgc aggcggcact gaccggacgc atcgtgtccc ggatcggcga gacccgggcg
     1981 atcctcgtcg gtatcgccgc agacgccatt gggctcatcg gccttgccct catcgccagc
     2041 acatgggcga tgctaccgat cctcgcagcg ctcggactcg gcagcatcac gttgcccgca
     2101 ctgcagacgc tgctctcgag acgcgcgccc gagcagcagc agggacgcct gcagggaaca
     2161 cttgcaagcc tgaacagcct cacctcgatc atcggcccgg tcaccttcac cggcattttc
     2221 gcactcaccc gaacgaatgc agacggcacc ctctggatct gcgccgcagc gctctacgtt
     2281 ctctgcgccc tcctgatgat ccgtgagaca tgcgcctcac ggcgatctcg ataaccgcgc
     2341 taaggtgcca tcccgatgcg acgggatcgc tctgccacca gtcaagtctc ccgtagccgg
     2401 tatgagggcc agcctcgcag agcaggattc ccgttgagca ccgccaggtg cgaataaggg
     2461 acagtgaaga aggaacaccc gctcgcgggt gggcctactt cacctatcct gccccgctga
     2521 cgccgttgga tacaccaagg aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt
     2581 gcgaaaaagg atggatatac cgaaaaaatc gctataatga ccccgaagca gggttatgca
     2641 gcggaaaagc catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     2701 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     2761 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2821 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     2881 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     2941 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3001 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3061 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagcatt
     3121 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3181 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3241 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3301 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     3361 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     3421 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     3481 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     3541 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     3601 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     3661 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     3721 attacgcc
//
