LOCUS       40924_42114        3549 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pT18mob2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Tauch A, Kirchner O, Loffler B, Gotker S, Puhler A, Kalinowski J.
  TITLE     Efficient electrotransformation of corynebacterium diphtheriae with 
            a mini-replicon derived from the Corynebacterium glutamicum plasmid 
            pGA1
  JOURNAL   Curr. Microbiol. 45 (5), 362-367 (2002)
  PUBMED    12232668
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Kirchner O, Tauch A.
  TITLE     Tools for genetic engineering in the amino acid-producing bacterium 
            Corynebacterium glutamicum
  JOURNAL   J. Biotechnol. 104 (1-3), 287-299 (2003)
  PUBMED    12948646
REFERENCE   3  (bases 1 to 3549)
  AUTHORS   Kirchner O, Tauch A.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2003) Department of Genetics, University of 
            Bielefeld, Universitaetsstrasse 25, Bielefeld D-33615, Germany
REFERENCE   4  (bases 1 to 3549)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 3549)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr. 
            Microbiol."; date: "2002"; volume: "45"; issue: "5"; pages: 
            "362-367"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "J. 
            Biotechnol. 104 (1-3), 287-299 (2003)"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (22-JAN-2003) Department of Genetics, University of Bielefeld, 
            Universitaetsstrasse 25, Bielefeld D-33615, Germany"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3549
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..57
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(61..77)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1017..1028)
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     oriT            complement(2232..2339)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      2551..3139
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3427..3448
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3463..3493
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3501..3517
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3525..3541
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttggc
       61 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
      121 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
      181 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc gataagctag cttcacgctg
      241 ccgcaagcac tcagggcgca agggctgcta aaggaagcgg aacacgtaga aagccagtcc
      301 gcagaaacgg tgctgacccc ggatgaatgt cagctactgg gctatctgga caagggaaaa
      361 cgcaagcgca aagagaaagc aggtagcttg cagtgggctt acatggcgat agctagactg
      421 ggcggtttta tggacagcaa gcgaaccgga attgccagct ggggcgccct ctggtaaggt
      481 tgggaagccc tgcaaagtaa actggatggc tttcttgccg ccaaggatct gatggcgcag
      541 gggatcaact ccttcgtcgg tgtcgtcgcc ggatggtctg cggtggtgct cagcgtggag
      601 acgcgcaccg tcacggaccg cgtgtagtgc gtggcggaaa cttcttgcgt ttcgcaagag
      661 aaatgcgtcc catttctcgt cggactcggg gaaggaagcg tgatgctctc ggtcaagcac
      721 gtcgctcgcc agcgctgcga ggagttcggc cttcgtgcgg aagtgccagt agaggccggg
      781 ctgctgtacc tgtaagtgag ccgccagcgc gcgagtggtg aagccatcga gcccagtctc
      841 gtcgagcacc tgccgggccc cgagcaacac ggacgtgcgg tcgagacgct tccggtggtg
      901 agtcatagtt gcactttatc atcgataact ttatcttaga taaagtgact gctcgctact
      961 ctcatctgac tgctcgctac tctcatcgtg gaatcctgac agccgtgctc atcacggcga
     1021 ccctcgatgc tgcagggctg ggcctcgtga tgccgatctt gcctaccctt ctcgaccagg
     1081 tcggtgcccc cgacgacatg atcccactgc acgtcggact actgacagcg ctctatgcga
     1141 tcatgcagtt tctttgcgcc ccgatccttg gccgactctc tgaccgtttc ggacgccgcc
     1201 gcgtgcttgt cgcctccctc gcaggcgcga cgatcgacta cctcgtgctc gcactgacgg
     1261 acacgctgtg ggtcttttac ctcgcccgcg cggttgcagg cattaccggc gccacgaacg
     1321 ccgtcaccgc gacggtgatc gccgacatta ctccgccgga tcagcgcgca aaacgctacg
     1381 ggtggctcgg cgcatgctac ggcggtggca tgatcgcggg tcccgccatt ggcggtcttt
     1441 tcggcggggt ctcaccgcat ctgccattcc tcgtcgccgc cgcgctcgcc ggaatcaccc
     1501 tcgtactcag cgcgagtctt ctgcgtgaga cgcggccacc gggcagcaac ggctcgcacg
     1561 cacagcaacc cggtacggcg aagcgaaccg cagtgccggg gatgcttatc cttctcgcag
     1621 tcttcggcat cgtgcagttc atcggccaag caccaggctc cacctgggtg ctcttcacgc
     1681 agcagcgcct cgactggaac cccgtcgaag tcggcgtttc gctatccatc ttcggaatgg
     1741 tgcaagtatt cgtgcaggcg gcactgaccg gacgcatcgt gtcccggatc ggcgagaccc
     1801 gggcgatcct cgtcggtatc gccgcagacg ccattgggct catcggcctt gccctcatcg
     1861 ccagcacatg ggcgatgcta ccgatcctcg cagcgctcgg actcggcagc atcacgttgc
     1921 ccgcactgca gacgctgctc tcgagacgcg cgcccgagca gcagcaggga cgcctgcagg
     1981 gaacacttgc aagcctgaac agcctcacct cgatcatcgg cccggtcacc ttcaccggca
     2041 ttttcgcact cacccgaacg aatgcagacg gcaccctctg gatctgcgcc gcagcgctct
     2101 acgttctctg cgccctcctg atgatccgtg agacatgcgc ctcacggcga tctcgataac
     2161 cgcgctaagg tgccatcccg atgcgacggg atcgctctgc caccagtcaa gtctcccgta
     2221 gccggtatga gggccagcct cgcagagcag gattcccgtt gagcaccgcc aggtgcgaat
     2281 aagggacagt gaagaaggaa cacccgctcg cgggtgggcc tacttcacct atcctgcccc
     2341 gctgacgccg ttggatacac caaggaaagt ctacacgaac cctttggcaa aatcctgtat
     2401 atcgtgcgaa aaaggatgga tataccgaaa aaatcgctat aatgaccccg aagcagggtt
     2461 atgcagcgga aaagccatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     2521 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2581 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2641 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     2701 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2761 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2821 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2881 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2941 gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     3001 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3061 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3121 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3181 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     3241 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     3301 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     3361 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     3421 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     3481 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     3541 catgattac
//
