LOCUS       40924_42084       10123 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pT14_T14x3_72bp-TALER21, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10123)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W, Li Z, Weiss R, Xie Z.
  TITLE     Modular construction of mammalian gene circuits using TALE 
            transcriptional repressors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10123)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2014) Bioinformatics Division/Center for Synthetic
REFERENCE   3  (bases 1 to 10123)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10123)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-SEP-2014) Bioinformatics Division/Center for Synthetic "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10123
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(222..810)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(984..1841)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1842..1946)
                     /label=AmpR promoter
     rep_origin      complement(1972..2427)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     2569..2585
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2595..2613
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    2628..3431
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    3510..3535
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    4207..4227
                     /label=attB4
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     protein_bind    4275..4369
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    4383..4399
                     /label=TALER14 bs binding site
                     /bound_moiety="TALER14 bs"
     misc_feature    4406..4465
                     /label=CMVmini
                     /note="CMVmini"
     protein_bind    4472..4488
                     /label=TALER14 bs binding site
                     /bound_moiety="TALER14 bs"
     protein_bind    4489..4505
                     /label=TALER14 bs binding site
                     /bound_moiety="TALER14 bs"
     protein_bind    4506..4522
                     /label=TALER14 bs binding site
                     /bound_moiety="TALER14 bs"
     protein_bind    4537..4561
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4614..7949
                     /label=similar to TALER21
                     /note="similar to TALER21"
     CDS             7959..7979
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     misc_feature    8025..8045
                     /label=FF3
                     /note="FF3"
     misc_feature    8051..8071
                     /label=FF3
                     /note="FF3"
     misc_feature    8077..8097
                     /label=FF3
                     /note="FF3"
     misc_feature    8103..8123
                     /label=FF3
                     /note="FF3"
     protein_bind    complement(8156..8180)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    8335..8390
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8751..8767)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8775..8791
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8799..8829)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8844..8865
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    9038..9874
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     promoter        complement(9904..9922)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9943..9959)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9967..9983
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9991..10021)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(10036..10057)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
       61 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
      121 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
      181 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
      241 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      301 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      361 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      421 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      481 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      541 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      601 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      661 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
      721 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
      781 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
      841 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
      901 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
      961 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     1021 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     1081 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     1141 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     1201 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     1261 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     1321 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     1381 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     1441 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     1501 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     1561 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     1621 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     1681 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     1741 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     1801 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     1861 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     1921 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
     1981 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     2041 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     2101 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     2161 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     2221 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     2281 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     2341 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     2401 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg
     2461 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     2521 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     2581 ccagtgagcg cgcgtaatac gactcactat agggcgaatt gggtacctgc tttctctgac
     2641 cagcattctc tcccctgggc ctgtgccgct ttctgtctgc agcttgtggc ctgggtcacc
     2701 tctacggctg gcccagatcc ttccctgccg cctccttcag gttccgtctt cctccactcc
     2761 ctcttcccct tgctctctgc tgtgttgctg cccaaggatg ctctttccgg agcacttcct
     2821 tctcggcgct gcaccacgtg atgtcctctg agcggatcct ccccgtgtct gggtcctctc
     2881 cgggcatctc tcctccctca cccaacccca tgccgtcttc actcgctggg ttcccttttc
     2941 cttctccttc tggggcctgt gccatctctc gtttcttagg atggccttct ccgacggatg
     3001 tctcccttgc gtcccgcctc cccttcttgt aggcctgcat catcaccgtt tttctggaca
     3061 accccaaagt accccgtctc cctggcttta gccacctctc catcctcttg ctttctttgc
     3121 ctggacaccc cgttctcctg tggattcggg tcacctctca ctcctttcat ttgggcagct
     3181 cccctacccc ccttacctct ctagtctgtg ctagctcttc cagccccctg tcatggcatc
     3241 ttccaggggt ccgagagctc agctagtctt cttcctccaa cccgggcccc tatgtccact
     3301 tcaggacagc atgtttgctg cctccaggga tcctgtgtcc ccgagctggg accaccttat
     3361 attcccaggg ccggttaatg tggctctggt tctgggtact tttatctgtc ccctccaccc
     3421 cacagtgggg caagcttatt accctgttat ccctaaccgg tgcgttttta tgcttgtagt
     3481 attgtataat gtttttaaga tccaagcttc tgacctcttc tcttcctccc acagggcctc
     3541 gagacaattg catcgatggt accagtgaaa gtctgcaatg aattcgagtt ggcgcgcctg
     3601 tcattctaaa tctctctttc agcctaaagc tttttccccg tatcccccca ggtgtctgca
     3661 ggctcaaaga gcagcgagaa gcgttcagag gaaagcgatc ccgtgccacc ttccccgtgc
     3721 ccgggctgtc cccgcacgct gccggctcgg ggatgcgggg ggagcgccgg accggagcgg
     3781 agccccgggc ggctcgctgc tgccccctag cgggggaggg acgtaattac atccctgggg
     3841 gctttgggag ggggctgtcc ccgtgagctc ccaggcgcgc ctgtcattct aaatctctct
     3901 ttcagcctaa agctttttcc ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga
     3961 gaagcgttca gaggaaagcg atcccgtgcc accttccccg tgcccgggct gtccccgcac
     4021 gctgccggct cggggatgcg gggggagcgc cggaccggag cggagccccg ggcggctcgc
     4081 tgctgccccc tagcggggga gggacgtaat tacatccctg ggggctttgg gggggggctg
     4141 tccccgtgag ctcccaggcg cgccaactcg gtaccgtatc gatgtcgacg ttaacgctag
     4201 tgatatcaac tttgtataga aaagttggct ccgaatttct cgacagatct catgtgatta
     4261 cgccaagcta cgggcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac
     4321 tgtcctccga gcggagtact gtcctccgag cggagttctg tcctccgagc ggagactcta
     4381 gatacccctc tccgcttctg aattctaggc gtgtacggtg ggaggcctat ataagcagag
     4441 ctcgtttagt gaaccgtcag atcgcctcga gtacccctct ccgcttctta cccctctccg
     4501 cttcttaccc ctctccgctt ctggatccaa ttcgacccaa gtttgtacaa aaaagcaggc
     4561 tgattaccgg agaattcgtg agcaagggcg ccaccatgac cggtttgtcg accattcgtc
     4621 cgcgcaggcc aagtcctgcc cgcgagcttc tgcccggacc ccaaccggat agggttcagc
     4681 cgactgcaga tcgtggggtg tctgcgcctg ctggcagccc tctggatggc ttgcccgctc
     4741 ggcggacggt gtcccggacc cggctgccat ctccccctgc gccctcacct gcgttctcgg
     4801 cgggcagctt cagcgatctg ctccgtccgt tcgatccgtc gcttcttgat acatcgcttc
     4861 ttgattcgat gcctgccgtc ggcacgccgc atacagcggc tgccccagca gagtgggatg
     4921 aggcgcaatc ggctctgcgt gcagccgatg acccgccacc caccgtgcgt gtcgctgtca
     4981 ctgccgcgcg gccgccgcgc gccaagccgg ccccgcgacg gcgtgctgcg caaccctccg
     5041 acgcttcgcc ggccgcgcag gtggatctac gcacgctcgg ctacagtcag cagcagcaag
     5101 agaagatcaa accgaaggtg cgttcgacag tggcgcagca ccacgaggca ctggtgggcc
     5161 atgggtttac acacgcgcac atcgttgcgc tcagccaaca cccggcagcg ttagggaccg
     5221 tcgctgtcac gtatcagcac ataatcacgg cgttgccaga ggcgacacac gaagacatcg
     5281 ttggcgtcgg caaacagtgg tccggcgcac gcgccctgga ggccttgctc acggatgcgg
     5341 gggagttgag aggtccgccg ttacagttgg acacaggcca acttgtgaag attgcaaaac
     5401 gtggcggcgt gaccgcaatg gaggcagtgc atgcatcgcg caatgcactg acgggtgccc
     5461 ccctgaacct gaccccggac caagtggtgg ctatcgccag caacaatggc ggcaagcaag
     5521 cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg
     5581 accaagtggt ggctatcgcc agcaacggtg gcggcaagca agcgctcgaa acggtgcagc
     5641 ggctgttgcc ggtgctgtgc caggaccatg gcctgactcc ggaccaagtg gtggctatcg
     5701 ccagccacga tggcggcaag caagcgctcg aaacggtgca gcggctgttg ccggtgctgt
     5761 gccaggacca tggcctgacc ccggaccaag tggtggctat cgccagcaac aatggcggca
     5821 agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac catggcctga
     5881 ccccggacca agtggtggct atcgccagca acggtggcgg caagcaagcg ctcgaaacgg
     5941 tgcagcggct gttgccggtg ctgtgccagg accatggcct gactccggac caagtggtgg
     6001 ctatcgccag ccacgatggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg
     6061 tgctgtgcca ggaccatggc ctgaccccgg accaagtggt ggctatcgcc agcaacggtg
     6121 gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg
     6181 gcctgacccc ggaccaagtg gtggctatcg ccagcaacgg tggcggcaag caagcgctcg
     6241 aaacggtgca gcggctgttg ccggtgctgt gccaggacca tggcctgact ccggaccaag
     6301 tggtggctat cgccagccac gatggcggca agcaagcgct cgaaacggtg cagcggctgt
     6361 tgccggtgct gtgccaggac catggcctga ccccggacca agtggtggct atcgccagca
     6421 acggtggcgg caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg
     6481 accatggcct gaccccggac caagtggtgg ctatcgccag caacaatggc ggcaagcaag
     6541 cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc ctgactccgg
     6601 accaagtggt ggctatcgcc agccacgatg gcggcaagca agcgctcgaa acggtgcagc
     6661 ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg gtggctatcg
     6721 ccagcaacat tggcggcaag caagcgctcg aaacggtgca gcggctgttg ccggtgctgt
     6781 gccaggacca tggcctgact ccggaccaag tggtggctat cgccagccac gatggcggca
     6841 agcaagcgct cgaaacggtg cagcggctgt tgccggtgct gtgccaggac catggcctga
     6901 ccccggacca agtggtggct atcgccagca acggtggcgg caagcaagcg ctcgaaacgg
     6961 tgcagcggct gttgccggtg ctgtgccagg accatggcct gaccccggac caagtggtgg
     7021 ctatcgccag caacggtggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg
     7081 tgctgtgcca ggaccatggc ctgaccccgg accaagtggt ggctatcgcc agcaacattg
     7141 gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg
     7201 gcctgacccc ggaccaagtg gtggctatcg ccagcaacgg tggcggcaag caagcgctcg
     7261 aaagcattgt ggcccagctg agccggcctg atccggcgtt ggccgcgttg accaacgacc
     7321 acctcgtcgc cttggcctgc ctcggcggac gtcctgccat ggatgcagtg aaaaagggat
     7381 tgccgcacgc gccggaattg atcagaagag tcaatcgccg tattggcgaa cgcacgtccc
     7441 atcgcgttgc cgactacgcg caagtggttc gcgtgctgga gtttttccag tgccactccc
     7501 acccagcgta cgcatttgat gaggccatga cgcagttcgg gatgagcagg aacgggttgg
     7561 tacagctctt tcgcagagtg ggcgtcaccg aactcgaagc ccgcggtgga acgctccccc
     7621 cagcctcgca gcgttgggac cgtatcctcc aggcatcagg gatgaaaagg gccaaaccgt
     7681 cccctacttc agctcaaaca ccggatcagg cgtctttgca tgcattcgcc gattcgctgg
     7741 agcgtgacct tgatgcgccc agcccaatgc acgagggaga tcagacgcgg gcaagcagcc
     7801 gtaaacggtc ccgatcggat cgtgctgtca ccggcccctc cgcacagcag gctgtcgagg
     7861 tgcgcgttcc cgaacagcgc gatgcgctgc atttgcccct cagctggagg gtaaaacgcc
     7921 cgcgtaccag gatctggggc ggcctcccgg atcccagccc caagaagaag agaaaggtgg
     7981 aggccagcgg tggcggctca aagcttacta gttaagcggc cgcaaacgat atgggctgaa
     8041 tacaatacca aacgatatgg gctgaataca ataccaaacg atatgggctg aatacaatac
     8101 caaacgatat gggctgaata caatacaagc ttgaggtgat ctagataact gatctaccca
     8161 gctttcttgt acaaagtggt acgcgtgaat tcactcctca ggtgcaggct gcctatcaga
     8221 aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct
     8281 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
     8341 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
     8401 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
     8461 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata
     8521 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga
     8581 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac
     8641 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc
     8701 ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg
     8761 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     8821 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     8881 ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tcgacagtac taagcttggt
     8941 ctaataccca atctctcgtc ttatccagat gttttatacg ccttaattaa tagggataac
     9001 agggtaatct cgaggtttaa acgtcgacgc ggccgctact agggacagga ttggtgacag
     9061 aaaagcccca tccttaggcc tcctccttcc tagtctcctg atattgggtc taacccccac
     9121 ctcctgttag gcagattcct tatctggtga cacaccccca tttcctggag ccatctctct
     9181 ccttgccaga acctctaagg tttgcttacg atggagccag agaggatcct gggagggaga
     9241 gcttggcagg gggtgggagg gaaggggggg atgcgtgacc tgcccggttc tcagtggcca
     9301 ccctgcgcta ccctctccca gaacctgagc tgctctgacg cggctgtctg gtgcgtttca
     9361 ctgatcctgg tgctgcagct tccttacact tcccaagagg agaagcagtt tggaaaaaca
     9421 aaatcagaat aagttggtcc tgagttctaa ctttggctct tcacctttct agtccccaat
     9481 ttatattgtt cctccgtgcg tcagttttac ctgtgagata aggccagtag ccagccccgt
     9541 cctggcaggg ctgtggtgag gaggggggtg tccgtgtgga aaactccctt tgtgagaatg
     9601 gtgcgtccta ggtgttcacc aggtcgtggc cgcctctact ccctttctct ttctccatcc
     9661 ttctttcctt aaagagtccc cagtgctatc tgggacatat tcctccgccc agagcagggt
     9721 cccgcttccc taaggccctg ctctgggctt ctgggtttga gtccttggca agcccaggag
     9781 aggcgctcag gcttccctgt cccccttcct cgtccaccat ctcatgcccc tggctctcct
     9841 gccccttccc tacaggggtt cctggctctg ctctccaccg cggtggagct ccagcttttg
     9901 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
     9961 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
    10021 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
    10081 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atc
//
