LOCUS       40924_42094       10089 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pT14_T14_72bp-TALER21, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10089)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W, Li Z, Weiss R, Xie Z.
  TITLE     Modular construction of mammalian gene circuits using TALE 
            transcriptional repressors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10089)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2014) Bioinformatics Division/Center for Synthetic
REFERENCE   3  (bases 1 to 10089)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10089)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-SEP-2014) Bioinformatics Division/Center for Synthetic "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(222..810)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(984..1841)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1842..1946)
                     /label=AmpR promoter
     rep_origin      complement(1972..2427)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     2569..2585
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2595..2613
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    2628..3431
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    3510..3535
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    4207..4227
                     /label=attB4
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     protein_bind    4275..4369
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    4383..4399
                     /label=TALER14 bs binding site
                     /bound_moiety="TALER14 bs"
     misc_feature    4406..4465
                     /label=CMVmini
                     /note="CMVmini"
     protein_bind    4472..4488
                     /label=TALER14 bs binding site
                     /bound_moiety="TALER14 bs"
     protein_bind    4503..4527
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4580..7915
                     /label=similar to TALER21
                     /note="similar to TALER21"
     CDS             7925..7945
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     misc_feature    7991..8011
                     /label=FF3
                     /note="FF3"
     misc_feature    8017..8037
                     /label=FF3
                     /note="FF3"
     misc_feature    8043..8063
                     /label=FF3
                     /note="FF3"
     misc_feature    8069..8089
                     /label=FF3
                     /note="FF3"
     protein_bind    complement(8122..8146)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    8301..8356
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8717..8733)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8741..8757
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8765..8795)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8810..8831
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    9004..9840
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     promoter        complement(9870..9888)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9909..9925)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9933..9949
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9957..9987)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(10002..10023)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
       61 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
      121 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
      181 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
      241 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
      301 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
      361 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
      421 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
      481 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
      541 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
      601 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
      661 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
      721 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
      781 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
      841 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
      901 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
      961 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     1021 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     1081 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     1141 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     1201 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     1261 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     1321 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     1381 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     1441 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     1501 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     1561 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     1621 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     1681 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     1741 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     1801 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     1861 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     1921 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa
     1981 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     2041 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     2101 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     2161 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     2221 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     2281 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     2341 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     2401 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg
     2461 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     2521 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     2581 ccagtgagcg cgcgtaatac gactcactat agggcgaatt gggtacctgc tttctctgac
     2641 cagcattctc tcccctgggc ctgtgccgct ttctgtctgc agcttgtggc ctgggtcacc
     2701 tctacggctg gcccagatcc ttccctgccg cctccttcag gttccgtctt cctccactcc
     2761 ctcttcccct tgctctctgc tgtgttgctg cccaaggatg ctctttccgg agcacttcct
     2821 tctcggcgct gcaccacgtg atgtcctctg agcggatcct ccccgtgtct gggtcctctc
     2881 cgggcatctc tcctccctca cccaacccca tgccgtcttc actcgctggg ttcccttttc
     2941 cttctccttc tggggcctgt gccatctctc gtttcttagg atggccttct ccgacggatg
     3001 tctcccttgc gtcccgcctc cccttcttgt aggcctgcat catcaccgtt tttctggaca
     3061 accccaaagt accccgtctc cctggcttta gccacctctc catcctcttg ctttctttgc
     3121 ctggacaccc cgttctcctg tggattcggg tcacctctca ctcctttcat ttgggcagct
     3181 cccctacccc ccttacctct ctagtctgtg ctagctcttc cagccccctg tcatggcatc
     3241 ttccaggggt ccgagagctc agctagtctt cttcctccaa cccgggcccc tatgtccact
     3301 tcaggacagc atgtttgctg cctccaggga tcctgtgtcc ccgagctggg accaccttat
     3361 attcccaggg ccggttaatg tggctctggt tctgggtact tttatctgtc ccctccaccc
     3421 cacagtgggg caagcttatt accctgttat ccctaaccgg tgcgttttta tgcttgtagt
     3481 attgtataat gtttttaaga tccaagcttc tgacctcttc tcttcctccc acagggcctc
     3541 gagacaattg catcgatggt accagtgaaa gtctgcaatg aattcgagtt ggcgcgcctg
     3601 tcattctaaa tctctctttc agcctaaagc tttttccccg tatcccccca ggtgtctgca
     3661 ggctcaaaga gcagcgagaa gcgttcagag gaaagcgatc ccgtgccacc ttccccgtgc
     3721 ccgggctgtc cccgcacgct gccggctcgg ggatgcgggg ggagcgccgg accggagcgg
     3781 agccccgggc ggctcgctgc tgccccctag cgggggaggg acgtaattac atccctgggg
     3841 gctttgggag ggggctgtcc ccgtgagctc ccaggcgcgc ctgtcattct aaatctctct
     3901 ttcagcctaa agctttttcc ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga
     3961 gaagcgttca gaggaaagcg atcccgtgcc accttccccg tgcccgggct gtccccgcac
     4021 gctgccggct cggggatgcg gggggagcgc cggaccggag cggagccccg ggcggctcgc
     4081 tgctgccccc tagcggggga gggacgtaat tacatccctg ggggctttgg gggggggctg
     4141 tccccgtgag ctcccaggcg cgccaactcg gtaccgtatc gatgtcgacg ttaacgctag
     4201 tgatatcaac tttgtataga aaagttggct ccgaatttct cgacagatct catgtgatta
     4261 cgccaagcta cgggcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac
     4321 tgtcctccga gcggagtact gtcctccgag cggagttctg tcctccgagc ggagactcta
     4381 gatacccctc tccgcttctg aattctaggc gtgtacggtg ggaggcctat ataagcagag
     4441 ctcgtttagt gaaccgtcag atcgcctcga gtacccctct ccgcttctgg atccaattcg
     4501 acccaagttt gtacaaaaaa gcaggctgat taccggagaa ttcgtgagca agggcgccac
     4561 catgaccggt ttgtcgacca ttcgtccgcg caggccaagt cctgcccgcg agcttctgcc
     4621 cggaccccaa ccggataggg ttcagccgac tgcagatcgt ggggtgtctg cgcctgctgg
     4681 cagccctctg gatggcttgc ccgctcggcg gacggtgtcc cggacccggc tgccatctcc
     4741 ccctgcgccc tcacctgcgt tctcggcggg cagcttcagc gatctgctcc gtccgttcga
     4801 tccgtcgctt cttgatacat cgcttcttga ttcgatgcct gccgtcggca cgccgcatac
     4861 agcggctgcc ccagcagagt gggatgaggc gcaatcggct ctgcgtgcag ccgatgaccc
     4921 gccacccacc gtgcgtgtcg ctgtcactgc cgcgcggccg ccgcgcgcca agccggcccc
     4981 gcgacggcgt gctgcgcaac cctccgacgc ttcgccggcc gcgcaggtgg atctacgcac
     5041 gctcggctac agtcagcagc agcaagagaa gatcaaaccg aaggtgcgtt cgacagtggc
     5101 gcagcaccac gaggcactgg tgggccatgg gtttacacac gcgcacatcg ttgcgctcag
     5161 ccaacacccg gcagcgttag ggaccgtcgc tgtcacgtat cagcacataa tcacggcgtt
     5221 gccagaggcg acacacgaag acatcgttgg cgtcggcaaa cagtggtccg gcgcacgcgc
     5281 cctggaggcc ttgctcacgg atgcggggga gttgagaggt ccgccgttac agttggacac
     5341 aggccaactt gtgaagattg caaaacgtgg cggcgtgacc gcaatggagg cagtgcatgc
     5401 atcgcgcaat gcactgacgg gtgcccccct gaacctgacc ccggaccaag tggtggctat
     5461 cgccagcaac aatggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct
     5521 gtgccaggac catggcctga ccccggacca agtggtggct atcgccagca acggtggcgg
     5581 caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct
     5641 gactccggac caagtggtgg ctatcgccag ccacgatggc ggcaagcaag cgctcgaaac
     5701 ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc ctgaccccgg accaagtggt
     5761 ggctatcgcc agcaacaatg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc
     5821 ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg gtggctatcg ccagcaacgg
     5881 tggcggcaag caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca
     5941 tggcctgact ccggaccaag tggtggctat cgccagccac gatggcggca agcaagcgct
     6001 cgaaacggtg cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca
     6061 agtggtggct atcgccagca acggtggcgg caagcaagcg ctcgaaacgg tgcagcggct
     6121 gttgccggtg ctgtgccagg accatggcct gaccccggac caagtggtgg ctatcgccag
     6181 caacggtggc ggcaagcaag cgctcgaaac ggtgcagcgg ctgttgccgg tgctgtgcca
     6241 ggaccatggc ctgactccgg accaagtggt ggctatcgcc agccacgatg gcggcaagca
     6301 agcgctcgaa acggtgcagc ggctgttgcc ggtgctgtgc caggaccatg gcctgacccc
     6361 ggaccaagtg gtggctatcg ccagcaacgg tggcggcaag caagcgctcg aaacggtgca
     6421 gcggctgttg ccggtgctgt gccaggacca tggcctgacc ccggaccaag tggtggctat
     6481 cgccagcaac aatggcggca agcaagcgct cgaaacggtg cagcggctgt tgccggtgct
     6541 gtgccaggac catggcctga ctccggacca agtggtggct atcgccagcc acgatggcgg
     6601 caagcaagcg ctcgaaacgg tgcagcggct gttgccggtg ctgtgccagg accatggcct
     6661 gaccccggac caagtggtgg ctatcgccag caacattggc ggcaagcaag cgctcgaaac
     6721 ggtgcagcgg ctgttgccgg tgctgtgcca ggaccatggc ctgactccgg accaagtggt
     6781 ggctatcgcc agccacgatg gcggcaagca agcgctcgaa acggtgcagc ggctgttgcc
     6841 ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg gtggctatcg ccagcaacgg
     6901 tggcggcaag caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca
     6961 tggcctgacc ccggaccaag tggtggctat cgccagcaac ggtggcggca agcaagcgct
     7021 cgaaacggtg cagcggctgt tgccggtgct gtgccaggac catggcctga ccccggacca
     7081 agtggtggct atcgccagca acattggcgg caagcaagcg ctcgaaacgg tgcagcggct
     7141 gttgccggtg ctgtgccagg accatggcct gaccccggac caagtggtgg ctatcgccag
     7201 caacggtggc ggcaagcaag cgctcgaaag cattgtggcc cagctgagcc ggcctgatcc
     7261 ggcgttggcc gcgttgacca acgaccacct cgtcgccttg gcctgcctcg gcggacgtcc
     7321 tgccatggat gcagtgaaaa agggattgcc gcacgcgccg gaattgatca gaagagtcaa
     7381 tcgccgtatt ggcgaacgca cgtcccatcg cgttgccgac tacgcgcaag tggttcgcgt
     7441 gctggagttt ttccagtgcc actcccaccc agcgtacgca tttgatgagg ccatgacgca
     7501 gttcgggatg agcaggaacg ggttggtaca gctctttcgc agagtgggcg tcaccgaact
     7561 cgaagcccgc ggtggaacgc tccccccagc ctcgcagcgt tgggaccgta tcctccaggc
     7621 atcagggatg aaaagggcca aaccgtcccc tacttcagct caaacaccgg atcaggcgtc
     7681 tttgcatgca ttcgccgatt cgctggagcg tgaccttgat gcgcccagcc caatgcacga
     7741 gggagatcag acgcgggcaa gcagccgtaa acggtcccga tcggatcgtg ctgtcaccgg
     7801 cccctccgca cagcaggctg tcgaggtgcg cgttcccgaa cagcgcgatg cgctgcattt
     7861 gcccctcagc tggagggtaa aacgcccgcg taccaggatc tggggcggcc tcccggatcc
     7921 cagccccaag aagaagagaa aggtggaggc cagcggtggc ggctcaaagc ttactagtta
     7981 agcggccgca aacgatatgg gctgaataca ataccaaacg atatgggctg aatacaatac
     8041 caaacgatat gggctgaata caataccaaa cgatatgggc tgaatacaat acaagcttga
     8101 ggtgatctag ataactgatc tacccagctt tcttgtacaa agtggtacgc gtgaattcac
     8161 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
     8221 caaataccac tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct
     8281 tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     8341 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa
     8401 tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg
     8461 ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca
     8521 tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc
     8581 tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg
     8641 actactccca gtcatagctg tccctcttct cttatggaga tccctcgacc tgcagcccaa
     8701 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     8761 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     8821 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     8881 agcggatcga cagtactaag cttggtctaa tacccaatct ctcgtcttat ccagatgttt
     8941 tatacgcctt aattaatagg gataacaggg taatctcgag gtttaaacgt cgacgcggcc
     9001 gctactaggg acaggattgg tgacagaaaa gccccatcct taggcctcct ccttcctagt
     9061 ctcctgatat tgggtctaac ccccacctcc tgttaggcag attccttatc tggtgacaca
     9121 cccccatttc ctggagccat ctctctcctt gccagaacct ctaaggtttg cttacgatgg
     9181 agccagagag gatcctggga gggagagctt ggcagggggt gggagggaag ggggggatgc
     9241 gtgacctgcc cggttctcag tggccaccct gcgctaccct ctcccagaac ctgagctgct
     9301 ctgacgcggc tgtctggtgc gtttcactga tcctggtgct gcagcttcct tacacttccc
     9361 aagaggagaa gcagtttgga aaaacaaaat cagaataagt tggtcctgag ttctaacttt
     9421 ggctcttcac ctttctagtc cccaatttat attgttcctc cgtgcgtcag ttttacctgt
     9481 gagataaggc cagtagccag ccccgtcctg gcagggctgt ggtgaggagg ggggtgtccg
     9541 tgtggaaaac tccctttgtg agaatggtgc gtcctaggtg ttcaccaggt cgtggccgcc
     9601 tctactccct ttctctttct ccatccttct ttccttaaag agtccccagt gctatctggg
     9661 acatattcct ccgcccagag cagggtcccg cttccctaag gccctgctct gggcttctgg
     9721 gtttgagtcc ttggcaagcc caggagaggc gctcaggctt ccctgtcccc cttcctcgtc
     9781 caccatctca tgcccctggc tctcctgccc cttccctaca ggggttcctg gctctgctct
     9841 ccaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg
     9901 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     9961 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
    10021 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
    10081 taatgaatc
//
