LOCUS       V002906                 4229 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002906
VERSION     V002906
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 4229)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4229)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..42
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             74..96
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     sig_peptide     104..166
                     /label="OmpA signal peptide"
                     /note="signal peptide from the E. coli outer membrane
                     protein OmpA"
     CDS             182..1639
                     /gene="P4hb"
                     /label="Protein disulfide-isomerase"
                     /note="Protein disulfide-isomerase from Rattus norvegicus.
                     Accession#: P04785"
     terminator      1691..1738
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     misc_feature    1943..2083
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2269..2857)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3031..3888)
                     /label="AmpR"
                     /note="beta-lactamase"
ORIGIN
        1 ttccggatct cgatcccgga aattaatacg actcactata gggagaccac aacggtttcc
       61 ctctagaaat aattttgttt aactttaaga aggagatata catatgaaaa agacagctat
      121 cgcgattgca gtggcactgg ctggtttcgc taccgtagcg caggccggcg ccgacgctct
      181 ggaggaggag gacaacgtcc tggtgctgaa gaagagcaac ttcgcagagc cggcggcgca
      241 caactacctg ctggtggagt tctatgcccc atggtgtggc cactgcaaag cactggcccc
      301 agagtatgcc aaagctgctg caaaactgaa ggcagaaggc tctgagatcc gactagcaaa
      361 ggtggacgcc acagaagagt ctgacctggc ccagcagtat ggtgtccgtg gctaccccac
      421 aatcaagttc ttcaagaatg gagacacagc ctccccaaag gaatatacag ctggcaggga
      481 agctgacgac attgtgaact ggctgaagaa acgcacaggc ccagcagcca caaccctgtc
      541 tgacactgca gctgcagagt ccttggtgga ctcaagcgaa gtgacggtca tcggcttctt
      601 caaggacgca gggtcagact ccgccaagca gttcttgctg gcagcagagg ctgttgatga
      661 catacctttt ggaatcactt ccaatagcga tgtgttttcc aagtaccagc tggacaagga
      721 tggggtggtc ctctttaaga agtttgatga aggccgcaac aattttgaag gtgagatcac
      781 caaggagaag ctattagact tcatcaagca caaccagctg cctttggtca tcgagttcac
      841 tgaacagaca gctccaaaga ttttcggagg tgaaatcaag acacatattc tgctgttcct
      901 gcccaagagt gtgtctgact acgatggcaa attgagcaac tttaagaaag cggccgaggg
      961 ctttaagggc aagatcctgt tcatcttcat cgatagtgac cacactgaca accagcgcat
     1021 acttgagttc tttggcctga agaaggagga atgtccagct gtgcggctta ttaccctgga
     1081 ggaagagatg accaagtaca aaccggagtc agacgagctg acagctgaga agatcacaca
     1141 attttgccac cacttcctgg agggcaagat caagccccac ctgatgagcc aggaactgcc
     1201 tgaagactgg gacaagcagc cagtgaaagt gctagttggg aaaaactttg aggaggttgc
     1261 ttttgatgag aaaaagaacg tgtttgttga attctatgct ccctggtgtg gtcactgcaa
     1321 gcagctagcc ccgatttggg ataaactggg agagacatac aaagaccatg agaatatcgt
     1381 catcgctaag atggactcaa cagccaatga ggtggaagct gtgaaggtgc acagctttcc
     1441 cacactcaag ttcttcccag caagtgcaga cagaacggtc attgattaca acggtgagcg
     1501 gacactagat ggttttaaga aattcttgga gagcggtggc caggatggag cgggggacaa
     1561 tgacgacctc gacctagaag aagctttaga gccagatatg gaagaagacg acgatcagaa
     1621 agccgtgaag gatgaactgt agtgcagaag ccagatccgg agagctccca acgcgttgga
     1681 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
     1741 gaaaggagga actatatgcg ctcatacgat atgaacgttg agactgccgc tgagttatca
     1801 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     1861 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     1921 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
     1981 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     2041 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc
     2101 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     2161 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     2221 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     2281 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     2341 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     2401 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     2461 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     2521 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     2581 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     2641 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     2701 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     2761 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     2821 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     2881 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     2941 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     3001 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     3061 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     3121 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     3181 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     3241 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     3301 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc
     3361 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     3421 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     3481 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     3541 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     3601 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc
     3661 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     3721 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     3781 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     3841 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     3901 tcaatattat gtaagcagac agttttattg ttcatgatga tatattttta tcttgtgcaa
     3961 tgtaacatca gagattttga gacacaacgt ggctttgttg aataaatcga acttttgctg
     4021 agttgaagga tcagatcacg catcttcccg acaacgcaga ccgttccgtg gcaaagcaaa
     4081 agttcaaaat caccaactgg tccacctaca acaaagctct catcaaccgt ggctccctca
     4141 ctttctggct ggatgatggg gcgattcagg cctggtatga gtcagcaaca ccttcttcac
     4201 gaggcagacc tcagcgccgg tgatgccaa
//
