LOCUS       40924_41964        7323 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7323)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7323)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2109..2480
                     /codon_start=1
                     /label=RelA (p65) AD
                     /note="transcriptional activation domain of human RelA,
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     polyA_signal    2750..2975
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3021..3449
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3463..3792
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3841..4863
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    4996..5129
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5166..5182)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5190..5206)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5214..5244)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5259..5280)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5568..6156)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6330..7187)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7188..7292)
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatcattc gagcttgcat gcctgcaggt cgaagcggag tactgtcctc
       61 cgagtttaaa agcggagtac tgtcctccga ggatatcagc ggagtactgt cctccgagtc
      121 gcgaagcgga gtactgtcct ccgagatcga tgtcgacccc gcccagcgtc ttgtcattgg
      181 cgaattcgaa cacgcagatg cagtcggggc ggcgcggtcc gaggtccact tcgcatatta
      241 aggtgacgcg tgtggcctcg aatcgcctgg agacgccatc cacgctgttt tgacctccat
      301 agaagacacc gggaccgatc cagcctccgc ggccgggaac ggtgcattgg aacgcggatt
      361 ccccgtgtta attaacaggt aagtgtcttc ctcctgtttc cttcccctgc tattctgctc
      421 aaccttccta tcagaaactg cagtatctgt atttttgcta gcagtaatac taacggttct
      481 ttttttctct tcacaggcca ccaagctacc ggtccaccat ggactcccag cagccagatc
      541 tgaagctact gtcttctatc gaacaagcat gcgatatttg ccgacttaaa aagctcaagt
      601 gctccaaaga aaaaccgaag tgcgccaagt gtctgaagaa caactgggag tgtcgctact
      661 ctcccaaaac caaaaggtct ccgctgacta gggcacatct gacagaagtg gaatcaaggc
      721 tagaaagact ggaacagcta tttctactga tttttcctcg agaagacctt gacatgattt
      781 tgaaaatgga ttctttacag gatataaaag cattgttaga attcccgggt gtcgaccaga
      841 aaaagttcaa taaagtcaga gttgtgagag cactggatgc tgttgctctc ccacagccag
      901 tgggcgttcc aaatgaaagc caagccctaa gccagagatt cactttttca ccaggtcaag
      961 acatacagtt gattccacca ctgatcaacc tgttaatgag cattgaacca gatgtgatct
     1021 atgcaggaca tgacaacaca aaacctgaca cctccagttc tttgctgaca agtcttaatc
     1081 aactaggcga gaggcaactt ctttcagtag tcaagtggtc taaatcattg ccaggttttc
     1141 gaaacttaca tattgatgac cagataactc tcattcagta ttcttggatg agcttaatgg
     1201 tgtttggtct aggatggaga tcctacaaac acgtcagtgg gcagatgctg tattttgcac
     1261 ctgatctaat actaaatgaa cagcggatga aagaatcatc attctattca ttatgcctta
     1321 ccatgtggca gatcccacag gagtttgtca agcttcaagt tagccaagaa gagttcctct
     1381 gtatgaaagt attgttactt cttaatacaa ttcctttgga agggctacga agtcaaaccc
     1441 agtttgagga gatgaggtca agctacatta gagagctcat caaggcaatt ggtttgaggc
     1501 aaaaaggagt tgtgtcgagc tcacagcgtt tctatcaact tacaaaactt cttgataact
     1561 tgcatgatct tgtcaaacaa cttcatctgt actgcttgaa tacatttatc cagtcccggg
     1621 cactgagtgt tgaatttcca gaaatgatgt ctgaagttat tgctgggtcg acgcccatgg
     1681 aattccagta cctgccagat acagacgatc gtcaccggat tgaggagaaa cgtaaaagga
     1741 catatgagac cttcaagagc atcatgaaga agagtccttt cagcggaccc accgaccccc
     1801 ggcctccacc tcgacgcatt gctgtgcctt cccgcagctc agcttctgtc cccaagccag
     1861 caccccagcc ctatcccttt acgtcatccc tgagcaccat caactatgat gagtttccca
     1921 ccatggtgtt tccttctggg cagatcagcc aggcctcggc cttggccccg gcccctcccc
     1981 aagtcctgcc ccaggctcca gcccctgccc ctgctccagc catggtatca gctctggccc
     2041 aggccccagc ccctgtccca gtcctagccc caggccctcc tcaggctgtg gccccacctg
     2101 cccccaagcc cacccaggct ggggaaggaa cgctgtcaga ggccctgctg cagctgcagt
     2161 ttgatgatga agacctgggg gccttgcttg gcaacagcac agacccagct gtgttcacag
     2221 acctggcatc cgtcgacaac tccgagtttc agcagctgct gaaccagggc atacctgtgg
     2281 ccccccacac aactgagccc atgctgatgg agtaccctga ggctataact cgcctagtga
     2341 caggggccca gaggcccccc gacccagctc ctgctccact gggggccccg gggctcccca
     2401 atggcctcct ttcaggagat gaagacttct cctccattgc ggacatggac ttctcagccc
     2461 tgctgagtca gatcagctcc taaggatcct ccggactaga aaagccgaat tctgcaggaa
     2521 ttgggtggca tccctgtgac ccctccccag tgcctctcct ggccctggaa gttgccactc
     2581 cagtgcccac cagccttgtc ctaataaaat taagttgcat cattttgtct gactaggtgt
     2641 ccttctataa tattatgggg tggagggggg tggtatggag caaggggcaa gttgggaaga
     2701 caacctgtag ggctcgaggg ggggcccgaa acccgctgat cagcctcgac tgtgccttct
     2761 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     2821 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     2881 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     2941 agcaaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     3001 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     3061 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     3121 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     3181 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     3241 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     3301 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     3361 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     3421 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     3481 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     3541 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     3601 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     3661 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     3721 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     3781 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg
     3841 atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
     3901 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
     3961 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
     4021 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
     4081 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
     4141 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
     4201 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
     4261 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
     4321 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
     4381 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
     4441 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     4501 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
     4561 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
     4621 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     4681 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     4741 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     4801 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
     4861 gaatagcacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
     4921 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
     4981 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     5041 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     5101 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     5161 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     5221 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     5281 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     5341 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     5401 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     5461 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     5521 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     5581 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     5641 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     5701 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     5761 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     5821 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     5881 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     5941 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     6001 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     6061 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     6121 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     6181 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     6241 aaaagaatct tcacctagat ccttttaaat taaaaatgaa gttttaatcc aatctaaagt
     6301 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     6361 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     6421 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     6481 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     6541 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     6601 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     6661 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     6721 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     6781 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     6841 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     6901 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     6961 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     7021 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     7081 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     7141 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     7201 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     7261 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     7321 gtc
//
