LOCUS       40924_41136        6014 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Episomal vector pSpiro-PAC-ADI-3xHA-C, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Jerlstrom-Hultqvist J, Einarsson E, Svard SG.
  TITLE     Stable Transfection of the Diplomonad Parasite Spironucleus 
            salmonicida
  JOURNAL   Eukaryotic Cell 11 (11), 1353-1361 (2012)
  PUBMED    22983987
REFERENCE   2  (bases 1 to 6014)
  AUTHORS   Jerlstroem-Hultqvist JSE., Einarsson EM, Svaerd SG.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2012) Department of Cell and Molecular Biology, 
            Uppsala University, BMC, Box 596, Uppsala 751 24, Sweden
REFERENCE   3  (bases 1 to 6014)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6014)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryotic 
            Cell"; date: "2012"; volume: "11"; issue: "11"; pages: "1353-1361"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-MAR-2012) Department of Cell and Molecular Biology, Uppsala 
            University, BMC, Box 596, Uppsala 751 24, Sweden"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6014
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /label=AmpR promoter
     CDS             129..986
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1160..1748
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2072..2102
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     3'UTR           complement(2214..2423)
                     /note="ornithine carbamoyltransferase from Spironucleus 
                     salmonicida"
     CDS             complement(2430..3023)
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     5'UTR           complement(3030..3191)
                     /note="ornithine carbamoyltransferase from Spironucleus 
                     salmonicida"
     5'UTR           3250..3562
                     /label=arginine deiminase from Spironucleus salmonicida
                     /note="arginine deiminase from Spironucleus salmonicida"
     CDS             5210..5290
                     /label=3xHA
                     /note="three tandem HA epitope tags"
     3'UTR           5297..5356
                     /note="ribosomal protein S15A from Spironucleus
                     salmonicida"
     promoter        complement(5371..5389)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5399..5415)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5557..6012
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct gggtaccact agtgttgtat
     2221 ggaatattta tatataaatg cattattaat aatgatatag tatcttaata tacattaata
     2281 atttgacaat tccaaacaaa aaagtaacat tattacaagt aataaagtat atattcatta
     2341 caaatatatt tcagataagt agtaactgtc tattcattat acaaatgagt aataaaagat
     2401 aattaatgaa atttagctga tcactcgagg gcaccgggct tgcgggtcat gcaccaggtc
     2461 gcgcggtcct tcgggcactc gacgtcggcg gtgacggtga agccgagccg ctcgtagaag
     2521 gggaggttgc ggggcgcgga ggtctccagg aaggcgggca ccccggcgcg ctcggccgcc
     2581 tccactccgg ggagcacgac ggcgctgccc agacccttgc cctggtggtc gggcgagacg
     2641 ccgacggtgg ccaggaacca cgcgggctcc ttgggccggt gcggcgccag gaggccttcc
     2701 atctgttgct gcgcggccag ccgggaaccg ctcaactcgg ccatgcgcgg gccgatctcg
     2761 gcgaacaccg cccccgcttc gacgctctcc ggcgtggtcc agaccgccac cgcggcgccg
     2821 tcgtccgcga cccacacctt gccgatgtcg agcccgacgc gcgtgaggaa gagttcttgc
     2881 agctcggtga cccgctcgat gtggcggtcc gggtcgacgg tgtggcgcgt ggcggggtag
     2941 tcggcgaacg cggcggcgag ggtgcgtacg gcccggggga cgtcgtcgcg ggtggcgagg
     3001 cgcaccgtgg gcttgtactc ggtgcccatg gtgggaagtg gtgaccaaat ctagaaaaaa
     3061 gatttaagaa tattatttgt taaattatta atctctatta tttccccctc cattatgttc
     3121 aaaaatatat ataaatatac cataattatt ctacttattt tgtgattttc ttaaatatgt
     3181 gagtatgcct ccatatgaag cttgcttaat taagcgctag cgatatcgga tcctgcagtc
     3241 tagacgcgtg cattatgcac taacctaatc tctccattaa tattatggac agaattaagt
     3301 taactgataa tttcctggat attggcttct taaggaacct caactgaaag cgtttctttc
     3361 gtttttagga ttttaactat gattttcatt ctagaggttt tatgtattat aattattatg
     3421 tagtataata ttataatgtt agttagcatt ataattattt tataacatat tataaaacct
     3481 gttaaaattc tcacaaattt aaaagtatct agtagaaatg tgagaatact tataattatt
     3541 ttactttcct tcaacaaatc aaatgtagat aaaaccctag taacattcag aacatgccac
     3601 cgctaggact atattagtat acgaacctga gctcgctgtt tttgtaaatt gccttcatcc
     3661 tgctggctcg ctctatgagt gcccaataaa ccctgagtca gccctagccc aacatcgtaa
     3721 tttccaaaaa gttctttaat cacatggtat taacgtattg gaagtttgtg aaatattgcg
     3781 ctccaacact actgatcctg ctcagcgtct agagttggag gaatacgcag catagtagct
     3841 cacatatatc ggcccagaag agtatgcaag tgatgactac aagatgaaag ttatcaaatc
     3901 actctctccg gagtcgcttg taaaggtaat tttaacagct cctgtagtgt atcttgaagc
     3961 cactacgaac aacactaacg ttatcactac aaagactgaa atgcgcgctc ttggtaacat
     4021 cgtgtttacg cgggattaat agattactac tgcaaagggt gtggtaatag gaaatttttc
     4081 agctgaatag cgtctaggtg agaacgcatt gatggaattc gtattaaaaa agattggaat
     4141 tagaccaatt ggtagaattg gagagttcac ttacgagaga cacatcccca tctgcggtgg
     4201 tgcaaacaag ttaactccta aagccactat tgaaggtggt gatttcatag ccttaaacaa
     4261 cgggatcggc gcacttggtg ttggactcag aagctcttat accgctggaa tccatcttat
     4321 ggagcacgac ctcctcggaa ctgacaagtt catcattgta aaagatacat tcgaccagga
     4381 ttaggataga atgcaccttg attgtacttg cagcccattg cacgacaaac tcattttgat
     4441 cgatgaagaa attttgatca atccggataa gctcagatat gtggacgagt gggttcactt
     4501 gggcggatat tcagatgagc ataagagctt ctacgcactc aatgaaaaga ggacgaacat
     4561 agaactgaag caatatttag aataatccgg atacagcatc atagccatcc ctcataagta
     4621 ctagctcgca tacggttgca atatgctcca tttgggattc acagatgggg cattccatat
     4681 tttaactgtc cacgaagaat ccaagctctt tatcgagcgc cacccagtgt tcaaaactta
     4741 ctgccagaaa aacaatatac gtattgtagt ggagtacgtt ccgttcaggt caattacgtc
     4801 catgtacggt tctttgcact gcgctacata ggtactcgaa agagacgttc caacagctgc
     4861 aattgataag tgtaactcct aggtgctcaa tgacgagaaa ctgttcgatg cagcactttt
     4921 tattccgact ttcttcgcat ccaaagagac tatcaaggaa gtctacgagc tttatgaaaa
     4981 gctcagaaag gagggaaaga atgtttacct catcaacaag tacaagatca cggagcttat
     5041 cagcttcaag aatctgaatg ccaagaatgt ggagggactc ctcgcagaaa tttacggcaa
     5101 gagcgccaaa aagcacttta tctaacccaa aaagcaggac ggggcagtta cagctattga
     5161 gcgcgacggg ctcagtcttg atgatatttt atacatcttg cggccgccct atccctacga
     5221 cgtgccagat tacgcatatc cgtacgatgt tcctgactat gcttacccct atgatgtccc
     5281 tgactacgcc gggccctgat taaatttgat ttttttaaat aaactattat tccaaaattt
     5341 tgatttatat tatttggagc tccaattcgc cctatagtga gtcgtattac gcgcgctcac
     5401 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     5461 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     5521 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     5581 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     5641 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     5701 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     5761 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     5821 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     5881 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     5941 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     6001 cgcttacaat ttag
//
