LOCUS       V003071                 5449 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003071
VERSION     V003071
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Jerlstrom-Hultqvist J, Einarsson E, Svard SG.
  TITLE     Stable Transfection of the Diplomonad Parasite Spironucleus
            salmonicida
  JOURNAL   Eukaryotic Cell 11 (11), 1353-1361 (2012)
   PUBMED   22983987
REFERENCE   2  (bases 1 to 5449)
  AUTHORS   Jerlstroem-Hultqvist JSE., Einarsson EM, Svaerd SG.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2012) Department of Cell and Molecular Biology,
            Uppsala University, BMC, Box 596, Uppsala 751 24, Sweden
REFERENCE   3  (bases 1 to 5449)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5449)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryotic
            Cell"; date: "2012"; volume: "11"; issue: "11"; pages: "1353-1361"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-MAR-2012) Department of Cell and Molecular Biology, Uppsala
            University, BMC, Box 596, Uppsala 751 24, Sweden"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5449
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /label="AmpR promoter"
     CDS             129..986
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1160..1748
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2036..2057
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2072..2102
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2171..2189
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     3'UTR           complement(2214..2423)
                     /note="ornithine carbamoyltransferase from Spironucleus
                     salmonicida"
     CDS             complement(2430..2849)
                     /gene="bsr"
                     /label="Blasticidin-S deaminase"
                     /note="Blasticidin-S deaminase from Bacillus cereus.
                     Accession#: P33967"
     5'UTR           complement(2847..3011)
                     /note="ornithine carbamoyltransferase from Spironucleus
                     salmonicida"
     5'UTR           3034..3147
                     /label="sec61 alpha from Spironucleus salmonicida"
                     /note="sec61 alpha from Spironucleus salmonicida"
     CDS             4642..4683
                     /label="OLLAS tag"
                     /note="Escherichia coli OmpF Linker and mouse Langerin
                     fusion sequence tag (Park et al., 2008)"
     CDS             4684..4725
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin
                     fusion sequence tag (Park et al., 2008)"
                     /label="OLLAS tag"
                     /translation="SGFANELGPRLMGK"
     3'UTR           4732..4791
                     /note="ribosomal protein S15A from Spironucleus
                     salmonicida"
     promoter        complement(4806..4824)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4834..4850)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      4992..5447
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct gggtaccact agtgttgtat
     2221 ggaatattta tatataaatg cattattaat aatgatatag tatcttaata tacattaata
     2281 atttgacaat tccaaacaaa aaagtaacat tattacaagt aataaagtat atattcatta
     2341 caaatatatt tcagataagt agtaactgtc tattcattat acaaatgagt aataaaagat
     2401 aattaatgaa atttagctga tcactcgaga tttcgggtat atttgagtgg aatgagttct
     2461 tcaatcgtag ttttgactaa cttgccattc atttctatta acacaaaaca atctggtgca
     2521 tagtctgaaa tcaactccct acacatacca caaggactta ccactcgaat acttctatct
     2581 acttcgtcag aataagggtg tctaacagct acaatcgtgt caaaatcctt ttgtccattc
     2641 gaaactgcac taccaatcgc aatggcttct gcacaaacag ttactcgtcc tatatacgct
     2701 tcaatatgta ctgccgaaat gatttctcct gttttcgtac gaattgccgc tcccacatga
     2761 tgtttattat cctcataaag cattgtaatc ttctctgtcg ctacttctac taattctaga
     2821 tcttgttgag aaatgttaaa ttgatccatg gtgggaagtg gtgaccaaat ctagaaaaaa
     2881 gatttaagaa tattatttgt taaattatta atctctatta tttccccctc cattatgttc
     2941 aaaaatatat ataaatatac cataattatt ctacttattt tgtgattttc ttaaatatgt
     3001 gagtatgcct ccatatgaag cttgcttaat taagagagct agaactactg gaagaacgaa
     3061 agatataaag acatatatat tcattaatag gtaattatta ttctataatt attaatataa
     3121 atataacttt aagaatttta gacataaatg agtattatat ataaatatag agtaatttat
     3181 aagtttagag gacacttcac ttcctctaaa atgtctccac cttcatggtt aaaagcgatt
     3241 gaaccatata ctaagctaat tccttatgta taaaaagctc ctagcactgt tactatagaa
     3301 ttaaagatgt tctacacatt tttatgttta acagtatttt tattaggctc tcaaattcct
     3361 ttatatggga tgagaactac tattgccaaa gaccctgttt tttggcttag aactattttt
     3421 tcttctgctc gtggatctgt tatggaatta ggattaggcc ctactatgac ttctggatta
     3481 atagtaagaa tgtgtgtttc agcaggttta cttagattca atagaaatga ttaagacgaa
     3541 actgaacttt ttggtagatt ttaatctctc gttggcgccg ctttcactat cggtcaggcc
     3601 gttttgtatg tcttcactgg tatgtatggt aactttgccg aactgggcgc cttcaatgct
     3661 atcgcaatcg tcctccagtt gtctggtgct tctattatct tgtagatgct ggaccacatg
     3721 atggagtagg gttggggcgt cgggtccggt tagtcacttt tcaccactgc ctagacttgt
     3781 gagtccatca tttggaaaac attttcgttc atgaaaattg acagaggcta cggcactgag
     3841 atcgagggtg caattcctgc agcgatctac atgattgtaa acagaggcaa caaactcgag
     3901 gccgtgaagc ttgcctttat acgtcatggc cttcccaact tggtagatgt catctccacc
     3961 ctggtaatat ttttcgttgt tgtgtacctt taaggaatta ggaaaaatat caagattgtt
     4021 cacgagcaag ctggcgacca gatgcagcag tcctacccca tcaagctcct ttatgcctca
     4081 tcaacgccca tgatgatcat atcaaccgtc actcaaaatg tgtttatgat atcctaggct
     4141 gtttggagaa aattaggcaa taattttgtc actggcattt taggcaagtg gcaggagaat
     4201 gaataaaacc cgggtagccc ttaccccgta ggcggccttg catggatttt agcccctccg
     4261 tattctttta gatctgccat attccacccc atacataccc tgcttcacgc tgttatcgtg
     4321 gtaacaatct ccggcttcgc tgccaagatg tgggttaact tttcagggga gtccgctaag
     4381 gacgttgctc agaaccttaa ggcctcaaag tggatcatac ccgggttcaa gcgcgaccag
     4441 gaaatggtca gggaactcaa cagatacatc ccagttgccg ctatgaccgg tggaatcatc
     4501 ttgggtctcc tcagcttgat tgctgatatt tctggctgct tagttagcgg ttcaggttta
     4561 cttatggcta caactagtct tgttaaaatg tatgaagact atgctaagaa ggctactggc
     4621 gctggatttt ggcggccgcc ctctgggttt gcaaatgaac tcggccctcg cctgatgggt
     4681 aagagcggct tcgccaacga gcttggaccc aggttgatgg gaaaggggcc ctgattaaat
     4741 ttgatttttt taaataaact attattccaa aattttgatt tatattattt ggagctccaa
     4801 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     4861 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     4921 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     4981 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     5041 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     5101 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     5161 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     5221 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     5281 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     5341 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     5401 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttag
//
