LOCUS       V003073                 5875 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003073
VERSION     V003073
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5875)
  AUTHORS   Jerlstrom-Hultqvist J, Einarsson E, Svard SG.
  TITLE     Stable Transfection of the Diplomonad Parasite Spironucleus
            salmonicida
  JOURNAL   Eukaryotic Cell 11 (11), 1353-1361 (2012)
   PUBMED   22983987
REFERENCE   2  (bases 1 to 5875)
  AUTHORS   Jerlstroem-Hultqvist JSE., Einarsson EM, Svaerd SG.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAR-2012) Department of Cell and Molecular Biology,
            Uppsala University, BMC, Box 596, Uppsala 751 24, Sweden
REFERENCE   3  (bases 1 to 5875)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5875)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Eukaryotic
            Cell"; date: "2012"; volume: "11"; issue: "11"; pages: "1353-1361"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (09-MAR-2012) Department of Cell and Molecular Biology, Uppsala
            University, BMC, Box 596, Uppsala 751 24, Sweden"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5875
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /label="AmpR promoter"
     CDS             129..986
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1160..1748
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2036..2057
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2072..2102
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2171..2189
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     3'UTR           complement(2214..2423)
                     /note="ornithine carbamoyltransferase from Spironucleus
                     salmonicida"
     CDS             complement(2430..2849)
                     /gene="bsr"
                     /label="Blasticidin-S deaminase"
                     /note="Blasticidin-S deaminase from Bacillus cereus.
                     Accession#: P33967"
     5'UTR           complement(2847..3011)
                     /note="ornithine carbamoyltransferase from Spironucleus
                     salmonicida"
     5'UTR           3034..3132
                     /label="BiP from Spironucleus salmonicida"
                     /note="BiP from Spironucleus salmonicida"
     CDS             5068..5109
                     /label="OLLAS tag"
                     /note="Escherichia coli OmpF Linker and mouse Langerin
                     fusion sequence tag (Park et al., 2008)"
     CDS             5110..5151
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin
                     fusion sequence tag (Park et al., 2008)"
                     /label="OLLAS tag"
                     /translation="SGFANELGPRLMGK"
     3'UTR           5158..5217
                     /note="ribosomal protein S15A from Spironucleus
                     salmonicida"
     promoter        complement(5232..5250)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5260..5276)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      5418..5873
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct gggtaccact agtgttgtat
     2221 ggaatattta tatataaatg cattattaat aatgatatag tatcttaata tacattaata
     2281 atttgacaat tccaaacaaa aaagtaacat tattacaagt aataaagtat atattcatta
     2341 caaatatatt tcagataagt agtaactgtc tattcattat acaaatgagt aataaaagat
     2401 aattaatgaa atttagctga tcactcgaga tttcgggtat atttgagtgg aatgagttct
     2461 tcaatcgtag ttttgactaa cttgccattc atttctatta acacaaaaca atctggtgca
     2521 tagtctgaaa tcaactccct acacatacca caaggactta ccactcgaat acttctatct
     2581 acttcgtcag aataagggtg tctaacagct acaatcgtgt caaaatcctt ttgtccattc
     2641 gaaactgcac taccaatcgc aatggcttct gcacaaacag ttactcgtcc tatatacgct
     2701 tcaatatgta ctgccgaaat gatttctcct gttttcgtac gaattgccgc tcccacatga
     2761 tgtttattat cctcataaag cattgtaatc ttctctgtcg ctacttctac taattctaga
     2821 tcttgttgag aaatgttaaa ttgatccatg gtgggaagtg gtgaccaaat ctagaaaaaa
     2881 gatttaagaa tattatttgt taaattatta atctctatta tttccccctc cattatgttc
     2941 aaaaatatat ataaatatac cataattatt ctacttattt tgtgattttc ttaaatatgt
     3001 gagtatgcct ccatatgaag cttgcttaat taagttcagt ctgctattcg ctcttgggcc
     3061 ctggaatgaa aatcgcttat tatattaaat ctatacaaaa gtattttcgc catttgacgt
     3121 atcccttcca caatgttcta gctacttcta gctgaggcca ttattggtat cgatttaggc
     3181 accacatatt cttgcgttgc agtatcaaga ggagggtagg ttgatattat acccaacgaa
     3241 atcggtaacc gtatcacccc ctcatatgta gcgttcaccg aggaaggaga gcgccttgtc
     3301 ggtgatgccg ccaaaaacta ggcttctctc aatccagaaa atacaatctt tgatattaag
     3361 cgcctcattg gccgtcgttt taacgatgcc tctgtgaaga aggatttacc ccacttgcca
     3421 tacaaggtcg tcaacattga taacaaaccg atcgtcgaag ttcgtgagat agaccctaaa
     3481 accaagaagg cagagattaa gtagtactct cctgaacaga tatctgcctt catccttatt
     3541 aagatgaagt agattgcgga ggagtacctt ggcgagacag tcaccaaagc aattgtaacc
     3601 gttcctgcat acttttcgga tgctcagcgt tctgccacta aagatgcagg tcgaattgct
     3661 ggactcgaga ttattcgtat tattaacgag ccaacagcag catctcttgc tttcggtgtc
     3721 gataaaaaag gtgaacggac tattttgaca tatgatcttg gtggtggtac ttttgatgtg
     3781 tctatccttt agatagagaa cggtgtattc gaagtgcttg ctaccaacgg agatacccat
     3841 ttaggaggcg aggattttga tcaacgcatt atcgaccact ttgttcaaat tttcaaatag
     3901 aaaaacggca tagatctcag aaagccagag cacagacgct tgtattctcg ccttaaacgt
     3961 gagaccgaaa atgcaaagcg cgttctctcc acccaacaga tgaccaaact ggagatggag
     4021 aacttttacg acggcaaaga tttttccgag acccttactc gcgctaagtt cgaggaactc
     4081 aacaacgatc ttttcaaaaa gacaatggat ccagttgcta atgttctcaa ggatgccaag
     4141 ctatagaagt cagatattga tgaaattgtt ctcgtgggag gctcaactag gatccctaag
     4201 attcaagccc tgctcaagga tttcttcaat aaagtcccta ataattccat ccatcccgat
     4261 gaggctgtcg gtgcgggagc tgctatttaa gctgccgccc tctctgggtc caacgagcat
     4321 gacattctgc ttatcgacgt tactcctctc accctcggta ttgagaccgt aggtggtgtc
     4381 atgacaccaa tcattgagcg caactcctac atcccaatca aaaagtccaa gaccttttct
     4441 actgtatagg atggacagac gatggttaat attactgttt acgagggcga acgctccatg
     4501 gttaaggaca ataaccttct cggcaacttt gaattggaag gtattcccgc cgctccccgt
     4561 ggtcaaccac aaatcgaggt tacttttgag ctagacgcga atggtatgct aactgtttct
     4621 gcagtagaga aaggatctgg ttaagaaaat gagattgtta tcaagaatga tcgtaaccga
     4681 ctttccgaag aagaaattga acgtctcatt cgtgaggccg aggagaacgc agacgaagac
     4741 aaaaagatca gagaaactgt agaattgaag aaccagttct aagcagctat ctatgatgta
     4801 aagagcaagc tcaattctga gaacgataaa aatatcctcg attccgtttc ggacgaggac
     4861 aaggatgtac tcgatgaagc tattgaagaa gccgaggcct ggatgatgga gaatgaggaa
     4921 gccgccaaag aggactttga agccgccatt gaaaagttcc agaccagcac ctagtcaatt
     4981 atgcgtaagt ataccaaggg ttctgcctct gcgcaaaccg aggtcgacga tgattttgat
     5041 cttaatgacg aactttggcg gccgccctct gggtttgcaa atgaactcgg ccctcgcctg
     5101 atgggtaaga gcggcttcgc caacgagctt ggacccaggt tgatgggaaa ggggccctga
     5161 ttaaatttga tttttttaaa taaactatta ttccaaaatt ttgatttata ttatttggag
     5221 ctccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     5281 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     5341 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     5401 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     5461 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     5521 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     5581 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     5641 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     5701 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     5761 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     5821 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttag
//
