LOCUS       V003075                 4570 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003075
VERSION     V003075
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Chen Z, Zhang J, Huang D.
  TITLE     Escherichia coli-Pseudomonas shuttle plasmid pSpcP-lac based
            spectinomycin resistant gene and lacZ alpha gene
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Chen Z, Zhang J, Huang D.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUN-2002) Biotechnology, Institute of Plant
            Protection, 2, Yuan Ming Yuan Xi Lu, HaiDian District, Beijing,
            Beijing 100094, China
REFERENCE   3  (bases 1 to 4570)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4570)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-JUN-2002) Biotechnology, Institute of Plant Protection, 2, Yuan
            Ming Yuan Xi Lu, HaiDian District, Beijing, Beijing 100094, China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(244..832)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             1128..1907
                     /gene="ant1"
                     /label="Spectinomycin 9-adenylyltransferase"
                     /note="Spectinomycin 9-adenylyltransferase from
                     Staphylococcus aureus (strain N315). Accession#: P0A0D1"
     primer_bind     4046..4062
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        4069..4087
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    4096..4203
                     /label="MCS"
                     /note="pBluescript multiple cloning site"
     promoter        complement(4216..4234)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4255..4271)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4279..4295)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4303..4333)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4348..4369)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 gatccccctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
       61 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
      121 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
      181 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
      241 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
      301 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      361 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
      421 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
      481 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
      541 taactatcgt cttgagtcca acccggtaag acacgactta tgcggactgg cagcagcaca
      601 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
      661 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
      721 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
      781 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
      841 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
      901 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttgactctag aggatcgatc
      961 tgtataataa agaataatta ttaatctgta gacaaattgt gaaaggatgt acttaaacgc
     1021 taacggtcag ctttattgaa cagtaattta agtatatgtc caatctaggg taagtaaatt
     1081 gagtatcaat ataaacttta tatgaacata atcaacgagg tgaaatcatg agcaatttga
     1141 ttaacggaaa aataccaaat caagcgattc aaacattaaa aatcgtaaaa gatttatttg
     1201 gaagttcaat agttggagta tatctatttg gttcagcagt aaatggtggt ttacgcatta
     1261 acagcgatgt agatgttcta gtcgtcgtga atcatagttt acctcaatta acacgaaaaa
     1321 aactaacaga aagactaatg actatatcag gaaagattgg aaatacggat tctgttagac
     1381 cacttgaagt tacggttata aataggagtg aagttgtccc ttggcaatat cctccaaaaa
     1441 gagaatttat atacggtgag tggctcaggg gtgaatttga gaatggacaa attcaggaac
     1501 caagctatga tcctgatttg gctattgttt tagcacaagc aagaaagaat agtatttctc
     1561 tatttggtcc tgattcttca agtatacttg tctccgtacc tttgacagat attcgaagag
     1621 caattaagga ttctttgcca gaactaattg aggggataaa aggtgatgag cgtaatgtaa
     1681 ttttaaccct agctcgaatg tggcaaacag tgactactgg tgaaattacc tcgaaagatg
     1741 tcgctgcaga atgggctata cctcttttac ctaaagagca tgtaacttta ctggatatag
     1801 ctagaaaagg ctatcgggga gagtgtgatg ataagtggga aggactatat tcaaaggtga
     1861 aagcactcgt taagtatatg aaaaattcta tagaaacttc tctcaattag gctaatttta
     1921 ttgcaataac aggtgcttac ttttaaaact actgatttat tgataaatca ccaccggtgc
     1981 cggagatccg gtctaggtcg ccgtagaccc aatcagctcg cgcccggcga agggtacaga
     2041 gtggtcccgc tcggacaaag cgctagagtc gtagacttta gaagggccgg aacgcgaagc
     2101 gaccccggaa tgggtggcgg aaccgcccga agaagccagg ttttgacttg ttgtctacac
     2161 actggaacgc gggccagaaa gcgacgcggg tgaggtggac atcgcccgac acgagcaact
     2221 agacgcagtg ccgacctagt tcgtgagcgt tgaacttcag gaactagctc cctatggccg
     2281 gaaggtcaac tttggtgaaa gcgtcgacca gttaaagata aagcgcgacc ggctacgaca
     2341 gggtaacgta ctcgtcgagc atttcggact agcgcaccca cgacaggtag aaccggtgca
     2401 gtcggttccg cataaaccac ttgacaaacc actcaaggca gtccatgccg tcgtacagaa
     2461 accacttgga ctcaagatgt gccgggagtg ggagggccat ctactaacaa acgtgggtcg
     2521 gccattagta gtgtgagcca gaaaagggga acggtaaccc gagaacccaa ttggcctgaa
     2581 gggcggcaaa gtccgcgtcc cggcgaagaa actcgaccaa catccttcta agctatccct
     2641 gtgggcggta gcagcgatac aggaggcggc agtgacttat gtagtgaagt agccactgtc
     2701 cgagcgagga gaagtggacc gattatgtcc ggtcttgcta ggcgacaagg acttgtgact
     2761 ccgctatgcg ccggagctgg tcccgtaacg aaaacatttg gtaaccccca ctccggtgca
     2821 agctgtaagg aacacatatt cccctgtgac atagacgcag ggtgttatgt tgtttaggca
     2881 gggaaatgtt gttgtttagg cagggaagaa ttgttgttta ggcagggaat taccgttgtt
     2941 taggcaggga aaaatttgag atgtccggtg cctaatgcac cggacatctg caggattttc
     3001 caaattttcc cttttccttc ttttcccacc tttgcgtttt ttgcgtggtg atgcaccggg
     3061 gcaaccccgg cgtaaacacg gggacttccc cgccccctcc gcagacccgt taggggcaaa
     3121 atggtcaggg gatagcggcg gactctcccg cgtccttcgc tcattagtcc catagctccg
     3181 cctaagtggg aaccgcaggt tggtcgccgt ggtcgccgcg gactctccgg atgtctcgcc
     3241 aacctgtggt tcccctcccc gattctggcc aaatagtcag gggaaaggga gcaaagaaag
     3301 gttgcgctat cgggtcgttc cggcggtggc aacggtggca gtggggtcgt tcgtgtcggt
     3361 caccgcacat taaccccaga ctgcgagtca ccttgctttt gagtgcaatt ccctaaaacc
     3421 agtactctaa tagtttttcc tagaagtgga tctaggaaaa tttaattttt acttcaaaat
     3481 ttagttagat ttcatatata ctcatttgaa ccagactgtc aatggttacg aattagtcac
     3541 tccgtggata gagtcgctag acagataaag caagtaggta tcaacggact gaggggcagc
     3601 acatctattg atgctatgcc ctcccgaatg gtagaccggg gtcacgacgt tactatggcg
     3661 ctctaggtgc gagtggccga ggtctaaata gtcgttattt ggtcggtcgg ccttcccggc
     3721 tcgcgtcttc accaggacgt tgaaataggc ggaggtaggt cagataatta acaacggccc
     3781 ttcgatctca ttcatcaagc ggtcaattat caaacgcgtt gcaacaacgg taacggcgga
     3841 ctacgccata aaagaggaat gcgtagacac gccataaagt gtggcgtaat taacgcttac
     3901 aatttccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     3961 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     4021 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattgta atacgactca
     4081 ctatagggcg aattggagct ccaccgcggt ggcggccgct ctagaactag tggatccccc
     4141 gggctgcagg aattcgatat caagcttatc gataccgtcg acctcgaggg ggggcccggt
     4201 acccagcttt tgttcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata
     4261 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     4321 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     4381 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     4441 acgcgcgggg agaggcggtt tgcgtattgg gcgcattagt gacatttgca tgcttcaaag
     4501 ccctgtcgaa ttggttttta gtgaaaagaa cacgatattc acggtttacc cacttataaa
     4561 caaaagatcg
//
