LOCUS       V014964                10771 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014964
VERSION     V014964
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10771)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10771
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            618..727
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             760..1128
                     /label="traJ"
                     /note="oriT-recognizing protein"
     rep_origin      1258..1969
                     /label="oriV"
                     /note="incP origin of replication"
     misc_feature    2469..2491
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     promoter        2682..2700
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    complement(2776..2952)
                     /label="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(3025..3540)
                     /note="RNA silencing suppressor p19 from Tomato bushy stunt
                     virus (strain Cherry). Accession#: P11690"
                     /label="RNA silencing suppressor p19"
     promoter        complement(3579..3924)
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     promoter        complement(3998..4016)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(4085..4115)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4130..4151)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4398..4426
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             4474..5661
                     /label="TcR"
                     /note="tetracycline efflux protein"
     CDS             6163..7308
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     CDS             8692..9660
                     /label="RepA"
                     /note="replication protein of plasmid pSa"
     CDS             10058..10705
                     /label="TetR"
                     /note="tetracycline resistance regulatory protein"
ORIGIN
        1 cccctttcca ccgcgtcaga cgcccgtagc agcccgctac gggctttttc atgccctgcc
       61 ctagcgtcca agcctcacgg ccgcgctcgg cctctctggc ggccttctgg cgctcttccg
      121 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
      181 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
      241 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
      301 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
      361 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
      421 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
      481 cgcttttccg ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca
      541 tcctttttcg cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt
      601 atccaacggc gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc
      661 ttcttcactg tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc
      721 tggccggcta ccgccggcgt aacagatgag ggcaagcgga tggctgatga aaccaagcca
      781 accaggaagg gcagcccacc tatcaaggtg tactgccttc cagacgaacg aagagcgatt
      841 gaggaaaagg cggcggcggc cggcatgagc ctgtcggcct acctgctggc cgtcggccag
      901 ggctacaaaa tcacgggcgt cgtggactat gagcacgtcc gcgagctggc ccgcatcaat
      961 ggcgacctgg gccgcctggg cggcctgctg aaactctggc tcaccgacga cccgcgcacg
     1021 gcgcggttcg gtgatgccac gatcctcgcc ctgctggcga agatcgaaga gaagcaggac
     1081 gagcttggca aggtcatgat gggcgtggtc cgcccgaggg cagagccatg acttttttag
     1141 ccgctaaaac ggccgggggg tgcgcgtgat tgccaagcac gtccccatgc gctccatcaa
     1201 gaagagcgac ttcgcggagc tggtgaagta catcaccgac gagcaaggca agaccgagcg
     1261 cctttgcgac gctcaccggg ctggttgccc tcgccgctgg gctggcggcc gtctatggcc
     1321 ctgcaaacgc gccagaaacg ccgtcgaagc cgtgtgcgag acaccgcggc cgccggcgtt
     1381 gtggatacct cgcggaaaac ttggccctca ctgacagatg aggggcggac gttgacactt
     1441 gaggggccga ctcacccggc gcggcgttga cagatgaggg gcaggctcga tttcggccgg
     1501 cgacgtggag ctggccagcc tcgcaaatcg gcgaaaacgc ctgattttac gcgagtttcc
     1561 cacagatgat gtggacaagc ctggggataa gtgccctgcg gtattgacac ttgaggggcg
     1621 cgactactga cagatgaggg gcgcgatcct tgacacttga ggggcagagt gctgacagat
     1681 gaggggcgca cctattgaca tttgaggggc tgtccacagg cagaaaatcc agcatttgca
     1741 agggtttccg cccgtttttc ggccaccgct aacctgtctt ttaacctgct tttaaaccaa
     1801 tatttataaa ccttgttttt aaccagggct gcgccctgtg cgcgtgaccg cgcacgccga
     1861 aggggggtgc ccccccttct cgaaccctcc cggcccgcta acgcgggcct cccatccccc
     1921 caggggctgc gcccctcggc cgcgaacggc ctcaccccaa aaatggcagc gctggcagtc
     1981 cttgccattg ccgggatcgg ggcagtaacg ggatgggcga tcagcccgag cgcgacgccc
     2041 ggaagcattg acgtgccgca ggtgctggca tcgacattca gcgaccaggt gccgggcagt
     2101 gagggcggcg gcctgggtgg cggcctgccc ttcacttcgg ccgtcggggc attcacggac
     2161 ttcatggcgg ggccggcaat ttttaccttg ggcattcttg gcatagtggt cgcgggtgcc
     2221 gtgctcgtgt tcgggggtgc gataaaccca gcgaaccatt tgaggtgata ggtaagatta
     2281 taccgaggta tgaaaacgag aattggacct ttacagaatt actctatgaa gcgccatatt
     2341 taaaaagcta ccaagacgaa gaggatgaag aggatgagga ggcagattgc cttgaatata
     2401 ttgacaatac tgataagata atatatcttt tatatagaag ataattcgag ctcggtaccc
     2461 ggggatcttg gcaggatata ttgtggtgta acgttaacat taacgcttac aatttccatt
     2521 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     2581 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     2641 cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg
     2701 gcgaattggg taccgggccc cccctcgagg tcgacggtat cgataagctt gatatcgaat
     2761 tcctgcagcc cgggggatct ggattttagt actggatttt ggttttagga attagaaatt
     2821 ttattgatag aagtatttta caaatacaaa tacatactaa gggtttctta tatgctcaac
     2881 acatgagcga aaccctataa gaaccctaat ttcccttatc gggaaactac tcacacatta
     2941 tttatggaga aaatagagag agatagattt gtagagagag actggtgatt tcagcgtacc
     3001 gaattcgagc tcgccctcga cttactcgct ttctttttcg aaggtttgag taccttcagg
     3061 gcatcctctt gatacattac tttccacttc gattggggca agctgtagca gttcttgctt
     3121 agaccgaatt gccatctcac agagatgctg aagagttcgc gaccctccag aaacggtgat
     3181 actaactcct cgaaaccgaa tactataggt acatccgatc tggtcgaaac cgaaaaatcg
     3241 agatgctgca tagttaaccg aatctcccgt ccaagatcca aggactctgt gcagtgaagc
     3301 ttccgtcctg tcgtatctga gatatctctt aaatacaact ttcccgaaac cccagctttc
     3361 cttgaaacca aggggattat cttgattcga attcgtctca tcgttatgta gccgccactc
     3421 agtccaactc ggactttcgt caggaagttt gaagggagaa gtggtacctc ctgatcctcc
     3481 atcccaacgt tcactgttag cttgttccct agcgtcgttt ccttgtatag ctcgttccat
     3541 atcgatttaa ggggatcctc tagagtcgaa gcttgggctg tcctctccaa atgaaatgaa
     3601 cttccttata tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg
     3661 tcagtggaga tgtcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt
     3721 tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct
     3781 tgaatgatag cctttccttt atcgcaatga tggcatttgt aggagccacc ttccttttct
     3841 actgtccttt cgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgaa
     3901 attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgaca
     3961 tttttggagt aggggtaccg agctccagct tttgttccct ttagtgaggg ttaattgcgc
     4021 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     4081 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     4141 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     4201 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     4261 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     4321 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     4381 tgaattaatt ctcatgtttg acagcttatc atcgattagc tttaatgcgg tagtttatca
     4441 cagttaaatt gctaacgcag tcaggcaccg tgtatgaaat ctaacaatgc gctcatcgtc
     4501 atcctcggca ccgtcaccct ggatgctgta ggcataggct tggttatgcc ggtactgccg
     4561 ggcctcttgc gggatatcgt ccattccgac agcatcgcca gtcactatgg cgtgctgcta
     4621 gcgctatatg cgttgatgca atttctatgc gcacccgttc tcggagcact gtccgaccgc
     4681 tttggccgcc gcccagtcct gctcgcttcg ctacttggag ccactatcga ctacgcgatc
     4741 atggcgacca cacccgtcct gtggattctc tacgccggac gcatcgtggc cggcatcacc
     4801 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
     4861 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
     4921 gccgggggac tgttgggcgc catctcctta catgcaccat tccttgcggc ggcggtgctc
     4981 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgc
     5041 cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg
     5101 actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg
     5161 gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc
     5221 ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc
     5281 gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg
     5341 ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt
     5401 ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat
     5461 gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc
     5521 actggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg
     5581 gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca
     5641 tggagccggg ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca
     5701 ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt
     5761 gtatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     5821 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatgtggcgc
     5881 aacgatgccg gcgacaagca ggagcgcacc gacttcttcc gcatcaagtg ttttggctct
     5941 caggccgagg cccacggcaa gtatttgggc aaggggtcgc tggtattcgt gcagggcaag
     6001 attcggaata ccaagtacga gaaggacggc cagacggtct acgggaccga cttcattgcc
     6061 gataaggtgg attatctgga caccaaggca ccaggcgggt caaatcagga ataagggcac
     6121 attgccccgg cgtgagtcgg ggcaatcccg caaggagggt gaatgaatcg gacgtttgac
     6181 cggaaggcat acaggcaaga actgatcgac gcggggtttt ccgccgagga tgccgaaacc
     6241 atcgcaagcc gcaccgtcat gcgtgcgccc cgcgaaacct tccagtccgt cggctcgatg
     6301 gtccagcaag ctacggccaa gatcgagcgc gacagcgtgc aactggctcc ccctgccctg
     6361 cccgcgccat cggccgccgt ggagcgttcg cgtcgtctcg aacaggaggc ggcaggtttg
     6421 gcgaagtcga tgaccatcga cacgcgagga actatgacga ccaagaagcg aaaaaccgcc
     6481 ggcgaggacc tggcaaaaca ggtcagcgag gccaagcagg ccgcgttgct gaaacacacg
     6541 aagcagcaga tcaaggaaat gcagctttcc ttgttcgata ttgcgccgtg gccggacacg
     6601 atgcgagcga tgccaaacga cacggcccgc tctgccctgt tcaccacgcg caacaagaaa
     6661 atcccgcgcg aggcgctgca aaacaaggtc attttccacg tcaacaagga cgtgaagatc
     6721 acctacaccg gcgtcgagct gcgggccgac gatgacgaac tggtgtggca gcaggtgttg
     6781 gagtacgcga agcgcacccc tatcggcgag ccgatcacct tcacgttcta cgagctttgc
     6841 caggacctgg gctggtcgat caatggccgg tattacacga aggccgagga atgcctgtcg
     6901 cgcctacagg cgacggcgat gggcttcacg tccgaccgcg ttgggcacct ggaatcggtg
     6961 tcgctgctgc accgcttccg cgtcctggac cgtggcaaga aaacgtcccg ttgccaggtc
     7021 ctgatcgacg aggaaatcgt cgtgctgttt gctggcgacc actacacgaa attcatatgg
     7081 gagaagtacc gcaagctgtc gccgacggcc cgacggatgt tcgactattt cagctcgcac
     7141 cgggagccgt acccgctcaa gctggaaacc ttccgcctca tgtgcggatc ggattccacc
     7201 cgcgtgaaga agtggcgcga gcaggtcggc gaagcctgcg aagagttgcg aggcagcggc
     7261 ctggtggaac acgcctgggt caatgatgac ctggtgcatt gcaaacgcta gggccttgtg
     7321 gggtcagttc cggctggggg ttcagcagcc agcgctttac tggcatttca ggaacaagcg
     7381 ggcactgctc gacgcacttg cttcgctcag tatcgctcgg gacgcacggc gcgctctacg
     7441 aactgccgat aaacagagga ttaaaattga caattgtgat taaggctcag attcgacggc
     7501 ttggagcggc cgacgtgcag gatttccgcg agatccgatt gtcggccctg aagaaagctc
     7561 cagagatgtt cgggtccgtt tacgagcacg aggagaaaaa gcccatggag gcgttcgctg
     7621 aacggttgcg agatgccgtg gcattcggcg cctacatcga cggcgagatc attgggctgt
     7681 cggtcttcaa acaggaggac ggccccaagg acgctcacaa ggcgcatctg tccggcgttt
     7741 tcgtggagcc cgaacagcga ggccgagggg tcgccggtat gctgctgcgg gcgttgccgg
     7801 cgggtttatt gctcgtgatg atcgtccgac agattccaac gggaatctgg tggatgcgca
     7861 tcttcatcct cggcgcactt aatatttcgc tattctggag cttgttgttt atttcggtct
     7921 accgcctgcc gggcggggtc gcggcgacgg taggcgctgt gcagccgctg atggtcgtgt
     7981 tcatctctgc cgctctgcta ggtagcccga tacgattgat ggcggtcctg ggggctattt
     8041 gcggaactgc gggcgtggcg ctgttggtgt tgacaccaaa cgcagcgcta gatcctgtcg
     8101 gcgtcgcagc gggcctggcg ggggcggttt ccatggcgtt cggaaccgtg ctgacccgca
     8161 agtggcaacc tcccgtgcct ctgctcacct ttaccgcctg gcaactggcg gccggaggac
     8221 ttctgctcgt tccagtagct ttagtgtttg atccgccaat cccgatgcct acaggaacca
     8281 atgttctcgg cctggcgtgg ctcggcctga tcggagcggg tttaacctac ttcctttggt
     8341 tccgggggat ctcgcgactc gaacctacag ttgtttcctt actgggcttt ctcagcccca
     8401 gatcctagag gatcgagcgg catttcattc aggcacgtac caccgaggcg ctacaagtcg
     8461 ccagagagcg cggcaagacg ctcgggcgac ccaagggcag caaatcgagc gccttgaagc
     8521 tggacagccg tattgatgaa gtacaggcat acgtgaacct tggcttgccg caaagtcgcg
     8581 cagccgagtt gttaggcgtc agccctcaca ccttgcgcct gttcatcaaa cgccggaaca
     8641 tcaaacccac aaacactaga ccaaccatca ccatgccggg gagggaacaa catgcctaag
     8701 aacaacaaag cccccggcca tcgtatcaac gagatcatca agacgagcct cgcgctcgaa
     8761 atggaggatg cccgcgaagc tggcttagtc ggctacatgg cccgttgcct tgtgcaagcg
     8821 accatgcccc acaccgaccc caagaccagc tactttgagc gcaccaatgg catcgtcacc
     8881 ttgtcgatca tgggcaagcc gagcatcggc ctgccctacg gttctatgcc gcgcaccttg
     8941 cttgcttgga tatgcaccga ggccgtgcga acgaaagacc ccgtgttgaa ccttggccgg
     9001 tcgcaatcgg aatttctaca aaggctcgga atgcacaccg atggccgtta cacggccacc
     9061 cttcgcaatc aggcgcaacg cctgttttca tccatgattt cgcttgccgg cgagcaaggc
     9121 aatgacttcg gcattgagaa cgtcgtcatt gccaagcgcg cttttctatt ctggaatccc
     9181 aagcggccag aagatcgggc gctatgggat agcaccctca ccctcacagg cgatttcttc
     9241 gaggaagtca cccgctcacc ggttcctatc cgaatcgact acctgcacgc cttgcggcag
     9301 tctccgcttg cgatggacat ttacacgtgg ctgacctatc gcgtgttcct gttgcgggcc
     9361 aagggccgcc ccttcgtgca aatcccttgg gtcgccctgc aagcgcaatt cggctcatcc
     9421 tatggcagcc gcgcacgcaa ctcgcccgaa ctggacgata aggcccgaga gcgggcagag
     9481 cgggcagcac tcgccagctt caaatacaac ttcaaaaagc gcctacgcga agtgttgatt
     9541 gtctatcccg aggcaagcga ctgcatcgaa gatgacggcg aatgcctgcg catcaaatcc
     9601 acacgcctgc atgtcacccg cgcacccggc aagggcgctc gcatcggccc ccctccgact
     9661 tgaccaggcc aacgctacgc ttggcttggt caaggatcga tctggcgccg gccagcgaga
     9721 cgagcaagat tggccgccgc ccgaaacgat ccgacagcgc gcccagcaca ggtgcgcagg
     9781 caaattgcac caacgcatac agcgccagca gaatgccata gtgggcggtg acgtcgttcg
     9841 agtgaaccag atcgcgcagg aggcccggca gcaccggcat aatcaggccg atgccgacag
     9901 cgtcgagcgc gacagtgctc agaattacga tcaggggtat gttgggtttc acgtctggcc
     9961 tccggaccag cctccgctgg tccgattgaa cgcgcggatt ctttatcact gataagttgg
    10021 tggacatatt atgtttatca gtgataaagt gtcaagcatg acaaagttgc agccgaatac
    10081 agtgatccgt gccgccctgg acctgttgaa cgaggtcggc gtagacggtc tgacgacacg
    10141 caaactggcg gaacggttgg gggttcagca gccggcgctt tactggcact tcaggaacaa
    10201 gcgggcgctg ctcgacgcac tggccgaagc catgctggcg gagaatcata cgcattcggt
    10261 gccgagagcc gacgacgact ggcgctcatt tctgatcggg aatgcccgca gcttcaggca
    10321 ggcgctgctc gcctaccgcg atggcgcgcg catccatgcc ggcacgcgac cgggcgcacc
    10381 gcagatggaa acggccgacg cgcagcttcg cttcctctgc gaggcgggtt tttcggccgg
    10441 ggacgccgtc aatgcgctga tgacaatcag ctacttcact gttggggccg tgcttgagga
    10501 gcaggccggc gacagcgatg ccggcgagcg cggcggcacc gttgaacagg ctccgctctc
    10561 gccgctgttg cgggccgcga tagacgcctt cgacgaagcc ggtccggacg cagcgttcga
    10621 gcagggactc gcggtgattg tcgatggatt ggcgaaaagg aggctcgttg tcaggaacgt
    10681 tgaaggaccg agaaagggtg acgattgatc aggaccgctg ccggagcgca acccactcac
    10741 tacagcagag ccatgtagac aacatcccct c
//
