LOCUS       40924_40652        4431 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pSlip7, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Qiu F, Guo L, Wen TJ, Liu F, Ashlock DA, Schnable PS.
  TITLE     DNA sequence-based 'bar codes' for tracking the origins of expressed
            sequence tags from a maize cDNA library constructed using multiple 
            mRNA sources
  JOURNAL   Plant Physiol. 133 (2), 475-481 (2003)
  PUBMED    14555776
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Liu F, Schnable PS.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2003) Iowa State University, B420 Agronomy Hall, 
            Ames, IA 50010, USA
REFERENCE   3  (bases 1 to 4431)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4431)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2003"; volume: "133"; issue: "2"; pages: "475-481"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2003) Iowa State University, B420 Agronomy Hall, Ames, IA 
            50010, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..458
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             746..1603
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     protein_bind    1708..1741
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      1819..2407
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2685..2762
                     /label=lacI promoter
     protein_bind    3857..3878
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3893..3923
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3931..3947
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        3979..3997
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4096..4113
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             4115..4132
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             4134..4151
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     promoter        complement(4251..4269)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4277..4293)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
       61 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
      121 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
      181 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
      241 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
      301 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
      361 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
      421 taacgcgaat tttaacaaaa tattaacgtt tacaatttca ggtggcactt ttcggggaaa
      481 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
      541 gccaggtctt ggactggtga gaacggcttg ctcggcagct tcgatgtgtg ctggagggag
      601 aataaaggtc taagatgtgc gatagaggga agtcgcattg aattatgtgc tgtgtaggga
      661 tcgctggtat caaatatgtg tgcccacccc tggcatgaga caataaccct gataaatgct
      721 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
      781 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
      841 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
      901 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
      961 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     1021 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     1081 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     1141 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     1201 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     1261 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     1321 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     1381 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     1441 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     1501 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     1561 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     1621 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     1681 gaagatcctt tttgataatc tcatgccata acttcgtata atgtatgcta tacgaagtta
     1741 tggcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     1801 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     1861 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     1921 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     1981 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     2041 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     2101 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     2161 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc attgagaaag
     2221 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     2281 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     2341 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     2401 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     2461 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     2521 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     2581 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     2641 cagagcttgc aattcgcgcg cgaaggcgaa gcggcattta cgttgacacc atcgaatggc
     2701 gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga
     2761 atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg
     2821 tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag
     2881 cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac
     2941 agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg
     3001 tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag
     3061 aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgggtca
     3121 gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct
     3181 gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta
     3241 ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc
     3301 agcaaatcgc gctgttagcg ggccattaag ttctgtctcg gcgcgtctgc gtctggctgg
     3361 ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg
     3421 gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac
     3481 tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc
     3541 cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc
     3601 atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag
     3661 cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc
     3721 cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg
     3781 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     3841 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     3901 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     3961 acagctgcta gccgaaatta atacgactca ctatagggag acaattgtga gcggacaaca
     4021 atttcacaca ggaaacagct atgatgacat gttatggcag atctttctcg ggaagcgcgc
     4081 cattgtgttg gtacccacca ccatcatcat cacgcatcac caccaccacc acgcatcatc
     4141 atcaccatca ccggtaccgg tccggaattc ccgggtcgac gagctcacta gtcggcggcc
     4201 gctctagagg atccaagctt acgtacgcgt gcatgcgacg tcatagctct tctatagtgt
     4261 cacctaaatt caattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     4321 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aaagcgaaga
     4381 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat g
//
