LOCUS       .                      11268 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             117..750
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    797..922
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1415..1648
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1833..1877
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2026..2067
                     /gene="Protein Tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    2164..2281
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2410..3588
                     /label="EF-1α promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1α"
     CDS             3608..4324
                     /label="EBFP2"
                     /note="enhanced blue variant of GFP (Ai et al., 2007)"
     CDS             5189..5896
                     /label="mOrange2"
                     /note="photostable monomeric orange derivative of DsRed
                     fluorescent protein (Shaner et al., 2008)"
     misc_feature    7584..8170
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             8190..8585
                     /label="BSD"
                     /note="blasticidin S deaminase"
     LTR             8791..9024
                     /label="3' LTR (ΔU3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9181..9407
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     promoter        9503..9607
                     /label="AmpR promoter"
     CDS             9608..10465
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      10639..11226
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 cggaccgcca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
       61 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga
      121 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca
      181 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
      241 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
      301 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
      361 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
      421 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
      481 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
      541 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
      601 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
      661 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
      721 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
      781 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
      841 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
      901 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
      961 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     1021 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     1081 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     1141 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     1201 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     1261 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     1321 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     1381 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     1441 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     1501 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     1561 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     1621 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     1681 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     1741 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     1801 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     1861 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     1921 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     1981 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     2041 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     2101 gatccattcg attagtgaac ggatctcgac ggtatcgcca caaatggcag tattcatcca
     2161 caattttaaa agaaaggggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     2221 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     2281 tcgggtttat tacagggaca gcagagatcc actttggatc gataagcttt gcaaagatgg
     2341 ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt cttgaaagga
     2401 gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     2461 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac
     2521 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     2581 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     2641 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg
     2701 ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga
     2761 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt
     2821 gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt
     2881 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt
     2941 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt
     3001 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg
     3061 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct
     3121 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg
     3181 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca
     3241 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg
     3301 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg
     3361 cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt
     3421 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac
     3481 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag
     3541 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagg aattgggatc
     3601 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     3661 gctggacggc gacgtaaacg gccacaagtt cagcgtgagg ggcgagggcg agggcgatgc
     3721 caccaacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     3781 gcccaccctc gtgaccaccc tgagccacgg cgtgcagtgc ttcgcccgct accccgacca
     3841 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     3901 catcttcttc aaggacgacg gcacctacaa gacccgcgcc gaggtgaagt tcgagggcga
     3961 caccctggtg aaccgcatcg agctgaaggg cgtcgacttc aaggaggacg gcaacatcct
     4021 ggggcacaag ctggagtaca acttcaacag ccacaacatc tatatcatgg ccgtcaagca
     4081 gaagaacggc atcaaggtga acttcaagat ccgccacaac gtggaggacg gcagcgtgca
     4141 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     4201 cagccactac ctgagcaccc agtccgtgct gagcaaagac cccaacgaga agcgcgatca
     4261 catggtcctg ctggagttcc gcaccgccgc cgggatcact ctcggcatgg acgagctgta
     4321 caagcggagg aagcgcgcac cggcaacaaa tttctcactg ctgaagcagg caggggatgt
     4381 cgaggagaac cctggtccca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     4441 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     4501 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     4561 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     4621 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     4681 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     4741 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     4801 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     4861 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     4921 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     4981 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     5041 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     5101 ggacgagctg tacaagaggc gcaagagggc acccgagggg cgaggcagcc tgctgacctg
     5161 cggcgatgtg gaggaaaacc caggcccaat ggtgagcaag ggcgaggaga ataacatggc
     5221 catcatcaag gagttcatgc gcttcaaggt gcgcatggag ggctccgtga acggccacga
     5281 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggctttcaga ccgctaagct
     5341 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcatttcac
     5401 ctacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tcaagctgtc
     5461 cttccccgag ggcttcaagt gggagcgcgt gatgaactac gaggacggcg gcgtggtgac
     5521 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
     5581 caccaacttc ccctccgacg gccccgtgat gcagaagaag accatgggct gggaggcctc
     5641 ctccgagcgg atgtaccccg aggacggtgc cctgaagggc aagatcaaga tgaggctgaa
     5701 gctgaaggac ggcggccact acacctccga ggtcaagacc acctacaagg ccaagaagcc
     5761 cgtgcagctg cccggcgcct acatcgtcga catcaagttg gacatcacct cccacaacga
     5821 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
     5881 ggacgagctg tacaagcgcc gcaaacgagc cccccagtgc accaactacg cgctcctgaa
     5941 gctggccgga gatgttgaga gcaacccggg accaatggtg agcaagggcg aggaggataa
     6001 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
     6061 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     6121 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
     6181 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
     6241 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     6301 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
     6361 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
     6421 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
     6481 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
     6541 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
     6601 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
     6661 cggcatggac gagctgtaca agcgccgaaa gcgagcgcct gtcaagcaga ccctgaactt
     6721 cgacctgctc aagctcgcgg gtgacgtgga aagcaatcca ggaccaatgg tgtctaaggg
     6781 cgaagagctg attaaggaga acatgcacat gaagctgtac atggagggca ccgtgaacaa
     6841 ccaccacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca cccagaccgg
     6901 cagaatcaag gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc tggctacctg
     6961 cttcatgtac ggcagcaaga ccttcatcaa ccacacccag ggcatccccg atttctttaa
     7021 gcagtccttc cctgagggct tcacatggga gagagtcacc acatacgaag acgggggcgt
     7081 gcttaccgtt acccaggaca ccagcctcca ggacggctgc ttgatctaca acgtcaagct
     7141 cagaggggtg aacttcccat ccaacggccc tgtgatgcag aagaaaacac tcggctggga
     7201 ggccagtacc gagaccctgt accccgctga cggcggcctg gaaggcagat gcgacatggc
     7261 cctgaagctc gtgggcgggg gccacctgca ctgcaacctg aagaccacat acagatccaa
     7321 gaaacccgct aagaacctca agatgcccgg cgtctacttt gtggaccgca gactggaaag
     7381 aatcaaggag gccgacaatg agacctacgt cgagcagcac gaggtggctg tggccagata
     7441 ctgcgacctc cctagcaaac tggggcacaa acttaatggc atggacgagc tgtacaagta
     7501 agaattcttc gaacatatga agcttgctag cactagtgat ccgcatgcat ctagggcggc
     7561 caattccgcc cctctccccc ccccccctct ccctcccccc cccctaacgt tactggccga
     7621 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg
     7681 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     7741 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     7801 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     7861 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     7921 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     7981 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     8041 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
     8101 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagct
     8161 tgccacaacc cacaaggaga cgaccttcca tggccaagcc tttgtctcaa gaagaatcca
     8221 ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg
     8281 tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt
     8341 ttactggggg accttgcgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg
     8401 gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg
     8461 gacggtgccg acaggttctt ctcgatctgc atcctgggat caaagccata gtgaaggaca
     8521 gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg
     8581 agggctaata ataggcggcc gctcgagacc tagaaaaaca tggagcaatc acaagtagca
     8641 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
     8701 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     8761 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     8821 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     8881 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     8941 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     9001 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     9061 aacttgcaaa gaaatgaata tcagagagtg agaggccttg acattataat agatttagca
     9121 ggaattgaac taggagtgga gcacacaggc aaagttctag agctcgctga tcagcctcga
     9181 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     9241 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     9301 tgagtaggtg tcattctatt ctggggggtg ggggtggggc aggacagcaa ggggggagga
     9361 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     9421 aagaaccagc tgggggcgcg cccctcgagg ccgccatggt catagctgtt tgacgtcagg
     9481 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     9541 aaatatgtat ccgctcacga gacaataacc ctgataaatg cttcaataat attgaaaaag
     9601 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     9661 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     9721 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     9781 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     9841 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     9901 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     9961 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
    10021 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
    10081 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
    10141 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
    10201 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
    10261 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
    10321 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
    10381 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
    10441 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
    10501 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
    10561 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
    10621 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
    10681 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
    10741 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
    10801 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
    10861 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
    10921 acgatagtta ccggataagc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
    10981 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
    11041 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
    11101 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
    11161 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
    11221 tggaaaaacg ccagcaacgc ggccttttta cggttctggc cttttgca
//
