LOCUS       62056_19790        4577 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(152..170)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(182..200)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(220..236)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(244..260)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(268..298)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(313..334)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(622..1210)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1384..2241)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2242..2346)
                     /label=AmpR promoter
     polyA_signal    complement(2636..2757)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2940..3536)
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     promoter        complement(3579..3936)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     4127..4143
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(4220..4460)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
ORIGIN
        1 atgatctggg aataggccct cttcctgccc gaccttggcg cgcgctcggc gcgcggtcac
       61 gctccgtcac gtggtgcgtt ttgcctgcgc gtctttccac tggggaattc atgcttctcc
      121 tccctttagt gagggtaatt ctctctctct ccctatagtg agtcgtatta attccttctc
      181 ttctatagtg tcacctaaat cgttgcaatt cgtaatcatg tcatagctgt ttcctgtgtg
      241 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
      301 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
      361 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
      421 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
      481 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
      541 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
      601 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
      661 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
      721 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
      781 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
      841 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
      901 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
      961 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     1021 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     1081 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaaa
     1141 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     1201 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     1261 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     1321 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     1381 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     1441 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     1501 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     1561 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     1621 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     1681 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     1741 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     1801 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     1861 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     1921 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     1981 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     2041 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     2101 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     2161 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     2221 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     2281 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     2341 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     2401 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga
     2461 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
     2521 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg
     2581 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgt ctagataaga
     2641 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     2701 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttggg
     2761 gtgggcgaag aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg
     2821 gaaaacgatt ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga
     2881 tggcaggttg ggcgtcgctt ggtcggtcat ttcgaacccc agagtcccgc gtcgactcag
     2941 gcaccgggct tgcgggtcat gcaccaggtg cgcggtcctt cgggcacctc gacgtcggcg
     3001 gtgacggtga agccgagccg ctcgtagaag gggaggttgc ggggcgcgga ggtctccagg
     3061 aaggcgggca ccccggcgcg ctcggccgcc tccactccgg ggagcacgac ggcgctgccc
     3121 agacccttgc cctggtggtc gggcgagacg ccgacggtgg ccaggaacca cgcgggctcc
     3181 ttgggccggt gcggcgccag gaggccttcc atctgttgct gcgcggccag ccgggaaccg
     3241 ctcaactcgg ccatgcgcgg gccgatctcg gcgaacaccg cccccgcttc gacgctctcc
     3301 ggcgtggtcc agaccgccac cgcggcgccg tcgtccgcga cccacacctt gccgatgtcg
     3361 agcccgacgc gcgtgaggaa gagttcttgc agctcggtga cccgctcgat gtggcggtcc
     3421 ggatcgacgg tgtggcgcgt ggcggggtag tcggcgaacg cggcggcgag ggtgcgtacg
     3481 gccctgggga cgtcgtcgcg ggtggcgagg cgcaccgtgg gcttgtactc ggtcatgccc
     3541 atgggcgaaa cgatcctcat cctgtctctt gatcgatctt tgcaaaagcc taggcctcca
     3601 aaaaagcctc ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca
     3661 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
     3721 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
     3781 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
     3841 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
     3901 tgacacacat tccacagctg gttctttccg cctcagcggt gtgaaatacc gcacagatgc
     3961 gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa
     4021 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     4081 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     4141 agtgccaagc ttttccaaaa aagcaggtct cagtgttaga atctcttgaa ttctaacact
     4201 gagacctgcg ggatcccgcg tcctttccac aagatatata aacccaagaa atcgaaatac
     4261 tttcaagtta cggtaagcat atgatagtcc attttaaaac ataattttaa aactgcaaac
     4321 tacccaagaa attattactt tctacgtcac gtattttgta ctaatatctt tgtgtttaca
     4381 gtcaaattaa ttctaattat ctctctaaca gccttgtatc gtatatgcaa atatgaagga
     4441 atcatgggaa ataggccctc ttcctgcccg accttggcgc gcgctcggcg cgcggtcacg
     4501 ctccgtcacg tggtgcgttt tgcctgcgcg tctttccact ggggaattca tgcttctcct
     4561 cccttaggag gcatccc
//
