LOCUS       62056_19785        6679 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6679)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(1342..1463)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(1586..1789)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(1790..2093)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        complement(2215..2233)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2245..2263)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(2283..2299)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2307..2323)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2331..2361)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2376..2397)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2685..3273)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3447..4304)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4305..4409)
                     /label=AmpR promoter
     polyA_signal    complement(4497..4578)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(4811..5407)
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     promoter        complement(5504..5820)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     6210..6226
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             join(6328..6679,1..1298)
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
ORIGIN
        1 tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa aaaattttga
       61 acgtgcaaaa aaagctccca atcatccaaa aaattattat catggattct aaaacggatt
      121 accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc ggttttaatg
      181 aatacgattt tgtgccagag tccttcgata gggacaagac aattgcactg atcatgaact
      241 cctctggatc tactggtctg cctaaaggtg tcgctctgcc tcatagaact gcctgcgtga
      301 gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat actgcgattt
      361 taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga tatttgatat
      421 gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgtttctg aggagccttc
      481 aggattacaa gattcaaagt gcgctgctgg tgccaaccct attctccttc ttcgccaaaa
      541 gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct ggtggcgctc
      601 ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca ggtatcaggc
      661 aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag ggggatgata
      721 aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg gatctggata
      781 ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt cctatgatta
      841 tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag gatggatggc
      901 tacattctgg agacatagct tactgggacg aagacgaaca cttcttcatc gttgaccgcc
      961 tgaagtctct gattaagtac aaaggctatc aggtggctcc cgctgaattg gaatccatct
     1021 tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg tcttcccgac gatgacgccg
     1081 gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg gaaaaagaga
     1141 tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga ggagttgtgt
     1201 ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga
     1261 tcctcataaa ggccaagaag ggcggaaaga tcgccgtgta attctagagt cggggcggcc
     1321 ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     1381 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     1441 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     1501 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg
     1561 ataaggatcc acggttcact aaaccagctc tgcttatata gacctcccac cgtacacgcc
     1621 taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat
     1681 tttggtgcca aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg
     1741 agtcaaaccg ctatccacgc ccattgatgt actgccaaaa ccgcatcacc atggtaatag
     1801 cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc atgtactggg
     1861 cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta cttggcatat
     1921 gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc cattgacgtc
     1981 aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac gtcaatgggc
     2041 gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta acgcggaact
     2101 ccatatatgg gctatgaact aatgaccccg taattgatta ctattaataa ctaagatctg
     2161 gtaccttgaa ttcatgcttc tcctcccttt agtgagggta attctctctc tctccctata
     2221 gtgagtcgta ttaattcctt ctcttctata gtgtcaccta aatcgttgca attcgtaatc
     2281 atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     2341 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     2401 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     2461 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     2521 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     2581 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     2641 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     2701 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     2761 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     2821 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     2881 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     2941 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     3001 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     3061 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     3121 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     3181 ggtagctctt gatccggcaa aaaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     3241 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     3301 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     3361 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     3421 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     3481 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     3541 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     3601 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     3661 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     3721 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     3781 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     3841 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     3901 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     3961 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     4021 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     4081 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     4141 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     4201 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     4261 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     4321 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     4381 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     4441 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gagattgcag
     4501 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     4561 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
     4621 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat
     4681 gatcctctag agtcggtggg cctcgggggc gggtgcgggg tcggcggggc cgccccgggt
     4741 ggcttcggtc ggagccatgg ggtcgtgcgc tcctttcggt cgggcgctgc gggtcgtggg
     4801 gcgggcgtca ggcaccgggc ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct
     4861 cgacgtcggc ggtgacggtg aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg
     4921 aggtctccag gaaggcgggc accccggcgc gctcggccgc ctccactccg gggagcacga
     4981 cggcgctgcc cagacccttg ccctggtggt cgggcgagac gccgacggtg gccaggaacc
     5041 acgcgggctc cttgggccgg tgcggcgcca ggaggccttc catctgttgc tgcgcggcca
     5101 gccgggaacc gctcaactcg gccatgcgcg ggccgatctc ggcgaacacc gcccccgctt
     5161 cgacgctctc cggcgtggtc cagaccgcca ccgcggcgcc gtcgtccgcg acccacacct
     5221 tgccgatgtc gagcccgacg cgcgtgagga agagttcttg cagctcggtg acccgctcga
     5281 tgtggcggtc cgggtcgacg gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga
     5341 gggtgcgtac ggcccggggg acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact
     5401 cggtcatgga aggtcgtctc cttgtgaggg gtcaggggcg tgggtcaggg gatggtggcg
     5461 gcaccggtcg tggcggccga cctgcaggca tgcaagctag ctttttgcaa aagcctaggc
     5521 ctccaaaaaa gcctcctcac tacttctgga atagctcaga ggccgaggcg gcctcggcct
     5581 ctgcataaat aaaaaaaatt agtcagccat ggggcggaga atgggcggaa ctgggcggag
     5641 ttaggggcgg gatgggcgga gttaggggcg ggactatggt tgctgactaa ttgagatgca
     5701 tgctttgcat acttctgcct gctggggagc ctggggactt tccacacctg gttgctgact
     5761 aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac tttccacacc
     5821 aatctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     5881 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     5941 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     6001 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     6061 catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     6121 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     6181 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctagcggc
     6241 cgcatacaaa aaaccaacac acagatccaa tgaaaataaa agatccttta ttaagcttgg
     6301 cattccggta ctgttggtaa agccaccatg gaagacgcca aaaacataaa gaaaggcccg
     6361 gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa ggctatgaag
     6421 agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga ggtggacatc
     6481 acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg
     6541 ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg
     6601 gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa
     6661 cgtgaattgc tcaacagta
//
