LOCUS       62056_19780        4553 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        402..492
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     promoter        complement(511..824)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das
                     et al., 1988)"
     primer_bind     complement(842..858)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(866..882)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(890..920)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(935..956)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1244..1832)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2006..2863)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    complement(2881..3014)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3193..3984)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(4051..4367)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt catatttgca tgtcgctatg
      421 tgttctggga aatcaccata aacgtgaaat gtctttggat ttgggaatct tataagttct
      481 gtatgagacc actcggatcc gaagaccaca aacaaggctt ttctccaagg gatatttata
      541 gtctcaaaac acacaattac tttacagtta gggtgagttt ccttttgtgc tgttttttaa
      601 aataataatt tagtatttgt atctcttata gaaatccaag cctatcatgt aaaatgtagc
      661 tagtattaaa aagaacagat tatctgtctt ttatcgcaca ttaagcctct atagttacta
      721 ggaaatatta tatgcaaatt aaccggggca ggggagtagc cgagcttctc ccacaagtct
      781 gtgcgagggg gccggcgcgg gcctagagat ggcggcgtcg gataagcttg gcgtaatcat
      841 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
      901 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
      961 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     1021 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     1081 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     1141 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     1201 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     1261 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     1321 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1381 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     1441 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     1501 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     1561 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     1621 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     1681 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     1741 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     1801 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     1861 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     1921 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     1981 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     2041 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     2101 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     2161 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     2221 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     2281 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     2341 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     2401 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     2461 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     2521 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     2581 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     2641 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     2701 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     2761 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     2821 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     2881 attcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     2941 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     3001 gcaataaaca agttggggtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc
     3061 atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa ggcggcggtg
     3121 gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc gcgaacccca
     3181 gagtcccgct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg
     3241 agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc
     3301 aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca
     3361 gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc
     3421 atgggtcacg acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc
     3481 ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc
     3541 catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc
     3601 cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg
     3661 agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct
     3721 tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca
     3781 cgatagccgc gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac
     3841 aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat
     3901 tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc
     3961 gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt gatcagatcc
     4021 gaaaatggat atacaagctc ccgggagctt tttgcaaaag cctaggcctc caaaaaagcc
     4081 tcctcactac ttctggaata gctcagaggc agaggcggcc tcggcctctg cataaataaa
     4141 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat
     4201 gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact
     4261 tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat
     4321 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacaccaat attattgaag
     4381 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     4441 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     4501 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtc
//
