LOCUS       40924_40147        5607 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pShuttle-SN, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5607)
  AUTHORS   Liu RY, Wu LZ, Huang BJ, Huang JL, Zhang YL, Ke ML, Wang JM, Tan WP,
            Zhang RH, Chen HK, Zeng YX, Huang W.
  TITLE     Adenoviral expression of a truncated S1 subunit of SARS-CoV spike 
            protein results in specific humoral immune responses against 
            SARS-CoV in rats
  JOURNAL   Virus Res. 112 (1-2), 24-31 (2005)
  PUBMED    16022898
REFERENCE   2  (bases 1 to 5607)
  AUTHORS   Liu R-Y., Huang B-J., Wu L-Z., Huang J-L., Zhang R-H., Zeng Y-X., 
            Huang W.
  TITLE     Constructing recombinant adenovirus carrying the spike gene 
            fragments as a vaccine against SARS-CoV by in vitro ligation
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 5607)
  AUTHORS   Liu R-Y., Huang B-J., Wu L-Z., Huang J-L., Zhang R-H., Zeng Y-X., 
            Huang W.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-DEC-2004) Cancer Center, Sun Yat-Sen University, 651 
            Dongfeng Road East, Guangzhou, Guangdong 510060, China
REFERENCE   4  (bases 1 to 5607)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5607)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virus Res. 
            112 (1-2), 24-31 (2005)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (21-DEC-2004) Cancer Center, Sun Yat-Sen University, 651 Dongfeng 
            Road East, Guangzhou, Guangdong 510060, China"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        262..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        890..908
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             990..2507
                     /codon_start=1
                     /product="truncated SARS coronavirus spike glycoprotein S1
                     subunit"
                     /label=truncated SARS coronavirus spike glycoprotein S
                     /protein_id="AAW56614.1"
                     /translation="MFIFLLFLTLTSGSDLDRCTTFDDVQAPNYTQHTSSMRGVYYPDE
                     IFRSDTLYLTQDLFLPFYSNVTGFHTINHTFDNPVIPFKDGIYFAATEKSNVVRGWVFG
                     STMNNKSQSVIIINNSTNVVIRACNFELCDNPFFAVSKPMGTQTHTMIFDNAFNCTFEY
                     ISDAFSLDVSEKSGNFKHLREFVFKNKDGFLYVYKGYQPIDVVRDLPSGFNTLKPIFKL
                     PLGINITNFRAILTAFSPAQDTWGTSAAAYFVGYLKPTTFMLKYDENGTITDAVDCSQN
                     PLAELKCSVKSFEIDKGIYQTSNFRVVPSGDVVRFPNITNLCPFGEVFNATKFPSVYAW
                     EGKKISNCVADYSVLYNSTFFSTFKCYGVSATKLNDLCFSNVYADSFVVKGDDVRQIAP
                     GQTGVIADYNYKLPDDFMGCVLAWNTRNIDATPTGNYNYKYRYLRHGKLRPFERDISNV
                     PFSPDGKPCTPPALNCYWPLNDYGFYTTTGIGTKLKFKPLISLDCAF"
     misc_feature    990..2459
                     /label=Region: SARS coronavirus spike glycoprotein
                     /note="Region: SARS coronavirus spike glycoprotein"
     polyA_signal    2495..2719
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(3055..3643)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4343..5155)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 taactataac ggtcctaagg tagcgaaagc tcagatctgg atctcccgat cccctatggt
       61 cgactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
      121 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      181 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      241 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggactat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      781 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      901 actataggga gacccaagct ggctagcgtt taaacgggcc ctctagagtt gtggtttcaa
      961 gtgatattct tgttaataac taaacgaaca tgtttatttt cttattattt cttactctca
     1021 ctagtggtag tgaccttgac cggtgcacca cttttgatga tgttcaagct cctaattaca
     1081 ctcaacatac ttcatctatg aggggggttt actatcctga tgaaattttt agatcagaca
     1141 ctctttattt aactcaggat ttatttcttc cattttattc taatgttaca gggtttcata
     1201 ctattaatca tacgtttgac aaccctgtca taccttttaa ggatggtatt tattttgctg
     1261 ccacagagaa atcaaatgtt gtccgtggtt gggtttttgg ttctaccatg aacaacaagt
     1321 cacagtcggt gattattatt aacaattcta ctaatgttgt tatacgagca tgtaactttg
     1381 aattgtgtga caaccctttc tttgctgttt ctaaacccat gggtacacag acacatacta
     1441 tgatattcga taatgcattt aattgcactt tcgagtacat atctgatgcc ttttcgcttg
     1501 atgtttcaga aaagtcaggt aattttaaac acttacgaga gtttgtgttt aaaaataaag
     1561 atgggtttct ctatgtttat aagggctatc aacctataga tgtagttcgt gatctacctt
     1621 ctggttttaa cactttgaaa cctattttta agttgcctct tggtattaac attacaaatt
     1681 ttagagccat tcttacagcc ttttcacctg cgcaagacac ttggggcacg tcagctgcag
     1741 cctattttgt tggctattta aagccaacta catttatgct caagtatgat gaaaatggta
     1801 caatcacaga tgctgttgat tgttctcaaa atccacttgc tgaactcaaa tgctctgtta
     1861 agagctttga gattgacaaa ggaatttacc agacctctaa tttcagggtt gttccctcag
     1921 gagatgttgt gagattccct aatattacaa acttgtgtcc ttttggagag gtttttaatg
     1981 ctactaaatt cccttctgtc tatgcatggg agggaaaaaa aatttctaat tgtgttgctg
     2041 attactctgt gctctacaac tcaacatttt tttcaacctt taagtgctat ggcgtttctg
     2101 ccactaagtt gaatgatctt tgcttctcca atgtctatgc agattctttt gtagtcaagg
     2161 gagatgatgt aagacaaata gcgccaggac aaactggtgt tattgctgat tataattata
     2221 aattgccaga tgatttcatg ggttgtgtcc ttgcttggaa tactaggaac attgatgcta
     2281 ctccaactgg taattataat tataaatata ggtatcttag acatggcaag cttaggccct
     2341 ttgagagaga catatctaat gtgcctttct cccctgatgg caaaccttgc accccacctg
     2401 ctcttaattg ttattggcca ttaaatgatt atggttttta caccactact ggcattggta
     2461 ccaagcttaa gtttaaaccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     2521 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     2581 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     2641 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     2701 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gcagatctgc agatctgaat
     2761 tcatctatgt cgggtgcgga gaaagaggta atgaaatggc attatgggta ttatgggtct
     2821 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     2881 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     2941 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     3001 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     3061 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     3121 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     3181 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     3241 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     3301 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     3361 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     3421 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     3481 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     3541 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     3601 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     3661 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     3721 attatcaaaa aggatcttca cctagatcct tttgatcctc cggcgttcag cctgtgccac
     3781 agccgacagg atggtgacca ccatttgccc catatcaccg tcggtactga tcccgtcgtc
     3841 aataaaccga accgctacac cctgagcatc aaactctttt atcagttgga tcatgtcggc
     3901 ggtgtcgcgg ccaagacggt cgagcttctt caccagaatg acatcacctt cctccacctt
     3961 catcctcagc aaatccagcc cttcccgatc tgttgaactg ccggatgcct tgtcggtaaa
     4021 gatgcggtta gcttttaccc ctgcatcttt gagcgctgag gtctgcctcg tgaagaaggt
     4081 gttgctgact cataccaggc ctgaatcgcc ccatcatcca gccagaaagt gagggagcca
     4141 cggttgatga gagctttgtt gtaggtggac cagttggtga ttttgaactt ttgctttgcc
     4201 acggaacggt ctgcgttgtc gggaagatgc gtgatctgat ccttcaactc agcaaaagtt
     4261 cgatttattc aacaaagccg ccgtcccgtc aagtcagcgt aatgctctgc cagtgttaca
     4321 accaattaac caattctgat tagaaaaact catcgagcat caaatgaaac tgcaatttat
     4381 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa
     4441 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc
     4501 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga
     4561 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc atttctttcc
     4621 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac
     4681 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac
     4741 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat
     4801 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag
     4861 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca
     4921 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac
     4981 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg
     5041 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca
     5101 tgttggaatt taatcgcggc ctcgagcaag acgtttcccg ttgaatatgg ctcataacac
     5161 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat
     5221 cttgtgcaat gtaacatcag agattttgag acacaacgtg gctttgttga ataaatcgaa
     5281 cttttgctga gttgaaggat cagatcacgc atcttcccga caacgcagac cgttccgtgg
     5341 caaagcaaaa gttcaaaatc accaactggt ccacctacaa caaagctctc atcaaccgtg
     5401 gctccctcac tttctggctg gatgatgggg cgattcaggc ctggtatgag tcagcaacac
     5461 cttcttcacg aggcagacct cagcgctaga ttattgaagc atttatcagg gttattgtct
     5521 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     5581 atttccccga aaagtgccac ctgacgt
//
