LOCUS       V013058                 9023 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013058
VERSION     V013058
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9023)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             69..860
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(911..1291)
                     /direction=LEFT
                     /label="M13 ori"
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     repeat_region   1329..1431
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     misc_signal     1519..1669
                     /label="Ad5 Psi"
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        1727..2030
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2031..2234
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2326..2406
                     /label="3xHA"
                     /note="three tandem HA epitope tags"
     misc_feature    2442..3025
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             3026..3742
                     /label="hrGFP"
                     /note="humanized Renilla green fluorescent protein"
     polyA_signal    3887..4008
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             4102..4521
                     /gene="IX"
                     /label="Hexon-interlacing protein"
                     /note="Hexon-interlacing protein from Human adenovirus C
                     serotype 5. Accession#: P03281"
     CDS             complement(4587..5921)
                     /gene="IVa2"
                     /label="Packaging protein 1"
                     /note="Packaging protein 1 from Human adenovirus C serotype
                     5. Accession#: P03271"
     CDS             complement(6506..6889)
                     /note="Early 4 ORF1 protein from Human adenovirus C
                     serotype 2. Accession#: P03242"
                     /label="Early 4 ORF1 protein"
     repeat_region   7199..7301
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     rep_origin      complement(7561..8149)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 ttccgccaag gatttgatgg gccaggggat caagctctga tcaagagaca ggatgaggat
       61 cgtttcgcat gattgaacaa gatggattgc acgcaggttt tccggccgct tgggtggaga
      121 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
      181 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
      241 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
      301 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
      361 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
      421 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
      481 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
      541 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
      601 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
      661 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
      721 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
      781 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
      841 gccttcttga cgagttcttc tgaattttgt taaaattttt gttaaatcag ctcatttttt
      901 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
      961 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     1021 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     1081 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     1141 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     1201 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     1261 gccgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggatc gaattaattc
     1321 ttaattaaca tcatcaataa tataccttat tttggattga agccaatatg ataatgaggg
     1381 ggtggagttt gtgacgtggc gcggggcgtg ggaacggggc gggtgacgta gtagtgtggc
     1441 ggaagtgtga tgttgcaagt gtggcggaac acatgtaagc gacggatgtg gcaaaagtga
     1501 cgtttttggt gtgcgccggt gtacacagga agtgacaatt ttcgcgcggt tttaggcgga
     1561 tgttgtagta aatttgggcg taaccgagta agatttggcc attttcgcgg gaaaactgaa
     1621 taagaggaag tgaaatctga ataattttgt gttactcata gcgcgtaata ctgtaatagt
     1681 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     1741 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     1801 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     1861 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     1921 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     1981 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     2041 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     2101 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     2161 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     2221 atataagcag agctggttta gtgaaccgtc agatccgcta gagatctgcg gccgcagtac
     2281 tgctagcact agtgatatcc gatcggtcga cgcccgggcc tcgagtaccc atatgacgtt
     2341 ccagactacg cgtatccgta cgacgttccg gattacgctt acccttacga tgtacctgac
     2401 tacgcttaat agcaattcct cgacgactgc atagggttac ccccctctcc ctcccccccc
     2461 cctaacgtta ctggccgaag tcgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     2521 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     2581 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     2641 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     2701 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     2761 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     2821 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     2881 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
     2941 tagtcgaggt taaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     3001 aaacacgatg ataatggcca caaccatggt gagcaagcag atcctgaaga acaccggcct
     3061 gcaggagatc atgagcttca aggtgaacct ggagggcgtg gtgaacaacc acgtgttcac
     3121 catggagggc tgcggcaagg gcaacatcct gttcggcaac cagctggtgc agatccgcgt
     3181 gaccaagggc gcccccctgc ccttcgcctt cgacatcctg agccccgcct tccagtacgg
     3241 caaccgcacc ttcaccaagt accccgagga catcagcgac ttcttcatcc agagcttccc
     3301 cgccggcttc gtgtacgagc gcaccctgcg ctacgaggac ggcggcctgg tggagatccg
     3361 cagcgacatc aacctgatcg aggagatgtt cgtgtaccgc gtggagtaca agggccgcaa
     3421 cttccccaac gacggccccg tgatgaagaa gaccatcacc ggcctgcagc ccagcttcga
     3481 ggtggtgtac atgaacgacg gcgtgctggt gggccaggtg atcctggtgt accgcctgaa
     3541 cagcggcaag ttctacagct gccacatgcg caccctgatg aagagcaagg gcgtggtgaa
     3601 ggacttcccc gagtaccact tcatccagca ccgcctggag aagacctacg tggaggacgg
     3661 cggcttcgtg gagcagcacg agaccgccat cgcccagctg accagcctgg gcaagcccct
     3721 gggcagcctg cacgagtggg tgtaataggc ggccatccga tccaccggat ctagataact
     3781 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     3841 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     3901 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     3961 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaac gcggatctgg
     4021 gcgtggttaa gggtgggaaa gaatatataa ggtgggggtc ttatgtagtt ttgtatctgt
     4081 tttgcagcag ccgccgccgc catgagcacc aactcgtttg atggaagcat tgtgagctca
     4141 tatttgacaa cgcgcatgcc cccatgggcc ggggtgcgtc agaatgtgat gggctccagc
     4201 attgatggtc gccccgtcct gcccgcaaac tctactacct tgacctacga gaccgtgtct
     4261 ggaacgccgt tggagactgc agcctccgcc gccgcttcag ccgctgcagc caccgcccgc
     4321 gggattgtga ctgactttgc tttcctgagc ccgcttgcaa gcagtgcagc ttcccgttca
     4381 tccgcccgcg atgacaagtt gacggctctt ttggcacaat tggattcttt gacccgggaa
     4441 cttaatgtcg tttctcagca gctgttggat ctgcgccagc aggtttctgc cctgaaggct
     4501 tcctcccctc ccaatgcggt ttaaaacata aataaaaaac cagactctgt ttggatttgg
     4561 atcaagcaag tgtcttgctg tctttattta ggggttttgc gcgcgcggta ggcccgggac
     4621 cagcggtctc ggtcgttgag ggtcctgtgt attttttcca ggacgtggta aaggtgactc
     4681 tggatgttca gatacatggg cataagcccg tctctggggt ggaggtagca ccactgcaga
     4741 gcttcatgct gcggggtggt gttgtagatg atccagtcgt agcaggagcg ctgggcgtgg
     4801 tgcctaaaaa tgtctttcag tagcaagctg attgccaggg gcaggccctt ggtgtaagtg
     4861 tttacaaagc ggttaagctg ggatgggtgc atacgtgggg atatgagatg catcttggac
     4921 tgtattttta ggttggctat gttcccagcc atatccctcc ggggattcat gttgtgcaga
     4981 accaccagca cagtgtatcc ggtgcacttg ggaaatttgt catgtagctt agaaggaaat
     5041 gcgtggaaga acttggagac gcccttgtga cctccaagat tttccatgca ttcgtccata
     5101 atgatggcaa tgggcccacg ggcggcggcc tgggcgaaga tatttctggg atcactaacg
     5161 tcatagttgt gttccaggat gagatcgtca taggccattt ttacaaagcg cgggcggagg
     5221 gtgccagact gcggtataat ggttccatcc ggcccagggg cgtagttacc ctcacagatt
     5281 tgcatttccc acgctttgag ttcagatggg gggatcatgt ctacctgcgg ggcgatgaag
     5341 aaaacggttt ccggggtagg ggagatcagc tgggaagaaa gcaggttcct gagcagctgc
     5401 gacttaccgc agccggtggg cccgtaaatc acacctatta ccggctgcaa ctggtagtta
     5461 agagagctgc agctgccgtc atccctgagc aggggggcca cttcgttaag catgtccctg
     5521 actcgcatgt tttccctgac caaatccgcc agaaggcgct cgccgcccag cgatagcagt
     5581 tcttgcaagg aagcaaagtt tttcaacggt ttgagaccgt ccgccgtagg catgcttttg
     5641 agcgtttgac caagcagttc caggcggtcc cacagctcgg tcacctgctc tacggcatct
     5701 cgatccagca tatctcctcg tttcgcgggt tggggcggct ttcgctgtac ggcagtagtc
     5761 ggtgctcgtc cagacgggcc agggtcatgt ctttccacgg gcgcagggtc ctcgtcagcg
     5821 tagtctgggt cacggtgaag gggtgcgctc cgggctgcgc gctggccagg gtgcgcttga
     5881 ggctggtcct gctggtgctg aagcgctgcc ggtcttcgcc ctgcgcgtcg gccaggtagc
     5941 atttgaccat ggtgtcatag tccagcccct ccgcggcgtg gcccttggcg cgcagcttgc
     6001 ccttggagga ggcgccgcac gaggggcagt gcagactttt gagggcgtag agcttgggcg
     6061 cgagaaatac cgattccggg gagtaggcat ccgcgccgca ggccccgcag acggtctcgc
     6121 attccacgag ccaggtgagc tctggccgtt cggggtcaaa aaccaggttt cccccatgct
     6181 ttttgatgcg tttcttacct ctggtttcca tgagccggtg tccacgctcg gtgacgaaaa
     6241 ggctgtccgt gtccccgtat acagacttga gagggagttt aaacgaattc aatagcttgt
     6301 tgcatgggcg gcgatataaa atgcaaggtg ctgctcaaaa aatcaggcaa agcctcgcgc
     6361 aaaaaagaaa gcacatcgta gtcatgctca tgcagataaa ggcaggtaag ctccggaacc
     6421 accacagaaa aagacaccat ttttctctca aacatgtctg cgggtttctg cataaacaca
     6481 aaataaaata acaaaaaaac atttaaacat tagaagcctg tcttacaaca ggaaaaacaa
     6541 cccttataag cataagacgg actacggcca tgccggcgtg accgtaaaaa aactggtcac
     6601 cgtgattaaa aagcaccacc gacagctcct cggtcatgtc cggagtcata atgtaagact
     6661 cggtaaacac atcaggttga ttcacatcgg tcagtgctaa aaagcgaccg aaatagcccg
     6721 ggggaataca tacccgcagg cgtagagaca acattacagc ccccatagga ggtataacaa
     6781 aattaatagg agagaaaaac acataaacac ctgaaaaacc ctcctgccta ggcaaaatag
     6841 caccctcccg ctccagaaca acatacagcg cttccacagc ggcagccata acagtcagcc
     6901 ttaccagtaa aaaagaaaac ctattaaaaa aacaccactc gacacggcac cagctcaatc
     6961 agtcacagtg taaaaaaggg ccaagtgcag agcgagtata tataggacta aaaaatgacg
     7021 taacggttaa agtccacaaa aaacacccag aaaaccgcac gcgaacctac gcccagaaac
     7081 gaaagccaaa aaacccacaa cttcctcaaa tcgtcacttc cgttttccca cgttacgtca
     7141 cttcccattt taagaaaact acaattccca acacatacaa gttactccgc cctaaaacct
     7201 acgtcacccg ccccgttccc acgccccgcg ccacgtcaca aactccaccc cctcattatc
     7261 atattggctt caatccaaaa taaggtatat tattgatgat gttaattaac atgcatggat
     7321 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc
     7381 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     7441 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     7501 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     7561 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     7621 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     7681 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     7741 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     7801 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     7861 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     7921 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     7981 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     8041 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     8101 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     8161 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     8221 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     8281 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     8341 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     8401 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     8461 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     8521 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     8581 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagc
     8641 catgagatta tcaaaaagga tcttcaccta gatccttttc acgtagaaag ccagtccgca
     8701 gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc
     8761 aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc
     8821 ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg
     8881 gaagccctgc aaagtaaact ggatggcttt cttgccgcca aggatctgat ggcgcagggg
     8941 atcaagctct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatg
     9001 cacgcagtct cggcacaaac taa
//
