LOCUS       V012203                 7469 bp    DNA     circular SYN 13-JAN-2022
DEFINITION  Exported.
ACCESSION   V012203
VERSION     V012203
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7469)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7469)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..103
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     misc_signal     191..341
                     /label="Ad5 Psi"
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        399..702
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        703..906
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     polyA_signal    1117..1238
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             1332..1751
                     /gene="IX"
                     /label="Hexon-interlacing protein"
                     /note="Hexon-interlacing protein from Human adenovirus C
                     serotype 5. Accession#: P03281"
     CDS             complement(1817..3151)
                     /gene="IVa2"
                     /label="Packaging protein 1"
                     /note="Packaging protein 1 from Human adenovirus C serotype
                     5. Accession#: P03271"
     CDS             complement(3736..4119)
                     /note="Early 4 ORF1 protein from Human adenovirus C
                     serotype 2. Accession#: P03242"
                     /label="Early 4 ORF1 protein"
     repeat_region   4429..4531
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     rep_origin      complement(4791..5379)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             6211..7002
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(7053..7432)
                     /direction=LEFT
                     /label="M13 ori"
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt
       61 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt
      121 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg
      181 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag
      241 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga
      301 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tactgtaata gtaatcaatt
      361 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
      421 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
      481 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
      541 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
      601 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
      661 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
      721 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
      781 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
      841 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
      901 agagctggtt tagtgaaccg tcagatccgc tagagatctg gtaccgtcga cgcggccgct
      961 cgagcctaag cttctagata agatatccga tccaccggat ctagataact gatcataatc
     1021 agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg
     1081 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
     1141 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     1201 tctagttgtg gtttgtccaa actcatcaat gtatcttaac gcggatctgg gcgtggttaa
     1261 gggtgggaaa gaatatataa ggtgggggtc ttatgtagtt ttgtatctgt tttgcagcag
     1321 ccgccgccgc catgagcacc aactcgtttg atggaagcat tgtgagctca tatttgacaa
     1381 cgcgcatgcc cccatgggcc ggggtgcgtc agaatgtgat gggctccagc attgatggtc
     1441 gccccgtcct gcccgcaaac tctactacct tgacctacga gaccgtgtct ggaacgccgt
     1501 tggagactgc agcctccgcc gccgcttcag ccgctgcagc caccgcccgc gggattgtga
     1561 ctgactttgc tttcctgagc ccgcttgcaa gcagtgcagc ttcccgttca tccgcccgcg
     1621 atgacaagtt gacggctctt ttggcacaat tggattcttt gacccgggaa cttaatgtcg
     1681 tttctcagca gctgttggat ctgcgccagc aggtttctgc cctgaaggct tcctcccctc
     1741 ccaatgcggt ttaaaacata aataaaaaac cagactctgt ttggatttgg atcaagcaag
     1801 tgtcttgctg tctttattta ggggttttgc gcgcgcggta ggcccgggac cagcggtctc
     1861 ggtcgttgag ggtcctgtgt attttttcca ggacgtggta aaggtgactc tggatgttca
     1921 gatacatggg cataagcccg tctctggggt ggaggtagca ccactgcaga gcttcatgct
     1981 gcggggtggt gttgtagatg atccagtcgt agcaggagcg ctgggcgtgg tgcctaaaaa
     2041 tgtctttcag tagcaagctg attgccaggg gcaggccctt ggtgtaagtg tttacaaagc
     2101 ggttaagctg ggatgggtgc atacgtgggg atatgagatg catcttggac tgtattttta
     2161 ggttggctat gttcccagcc atatccctcc ggggattcat gttgtgcaga accaccagca
     2221 cagtgtatcc ggtgcacttg ggaaatttgt catgtagctt agaaggaaat gcgtggaaga
     2281 acttggagac gcccttgtga cctccaagat tttccatgca ttcgtccata atgatggcaa
     2341 tgggcccacg ggcggcggcc tgggcgaaga tatttctggg atcactaacg tcatagttgt
     2401 gttccaggat gagatcgtca taggccattt ttacaaagcg cgggcggagg gtgccagact
     2461 gcggtataat ggttccatcc ggcccagggg cgtagttacc ctcacagatt tgcatttccc
     2521 acgctttgag ttcagatggg gggatcatgt ctacctgcgg ggcgatgaag aaaacggttt
     2581 ccggggtagg ggagatcagc tgggaagaaa gcaggttcct gagcagctgc gacttaccgc
     2641 agccggtggg cccgtaaatc acacctatta ccggctgcaa ctggtagtta agagagctgc
     2701 agctgccgtc atccctgagc aggggggcca cttcgttaag catgtccctg actcgcatgt
     2761 tttccctgac caaatccgcc agaaggcgct cgccgcccag cgatagcagt tcttgcaagg
     2821 aagcaaagtt tttcaacggt ttgagaccgt ccgccgtagg catgcttttg agcgtttgac
     2881 caagcagttc caggcggtcc cacagctcgg tcacctgctc tacggcatct cgatccagca
     2941 tatctcctcg tttcgcgggt tggggcggct ttcgctgtac ggcagtagtc ggtgctcgtc
     3001 cagacgggcc agggtcatgt ctttccacgg gcgcagggtc ctcgtcagcg tagtctgggt
     3061 cacggtgaag gggtgcgctc cgggctgcgc gctggccagg gtgcgcttga ggctggtcct
     3121 gctggtgctg aagcgctgcc ggtcttcgcc ctgcgcgtcg gccaggtagc atttgaccat
     3181 ggtgtcatag tccagcccct ccgcggcgtg gcccttggcg cgcagcttgc ccttggagga
     3241 ggcgccgcac gaggggcagt gcagactttt gagggcgtag agcttgggcg cgagaaatac
     3301 cgattccggg gagtaggcat ccgcgccgca ggccccgcag acggtctcgc attccacgag
     3361 ccaggtgagc tctggccgtt cggggtcaaa aaccaggttt cccccatgct ttttgatgcg
     3421 tttcttacct ctggtttcca tgagccggtg tccacgctcg gtgacgaaaa ggctgtccgt
     3481 gtccccgtat acagacttga gagggagttt aaacgaattc aatagcttgt tgcatgggcg
     3541 gcgatataaa atgcaaggtg ctgctcaaaa aatcaggcaa agcctcgcgc aaaaaagaaa
     3601 gcacatcgta gtcatgctca tgcagataaa ggcaggtaag ctccggaacc accacagaaa
     3661 aagacaccat ttttctctca aacatgtctg cgggtttctg cataaacaca aaataaaata
     3721 acaaaaaaac atttaaacat tagaagcctg tcttacaaca ggaaaaacaa cccttataag
     3781 cataagacgg actacggcca tgccggcgtg accgtaaaaa aactggtcac cgtgattaaa
     3841 aagcaccacc gacagctcct cggtcatgtc cggagtcata atgtaagact cggtaaacac
     3901 atcaggttga ttcacatcgg tcagtgctaa aaagcgaccg aaatagcccg ggggaataca
     3961 tacccgcagg cgtagagaca acattacagc ccccatagga ggtataacaa aattaatagg
     4021 agagaaaaac acataaacac ctgaaaaacc ctcctgccta ggcaaaatag caccctcccg
     4081 ctccagaaca acatacagcg cttccacagc ggcagccata acagtcagcc ttaccagtaa
     4141 aaaagaaaac ctattaaaaa aacaccactc gacacggcac cagctcaatc agtcacagtg
     4201 taaaaaaggg ccaagtgcag agcgagtata tataggacta aaaaatgacg taacggttaa
     4261 agtccacaaa aaacacccag aaaaccgcac gcgaacctac gcccagaaac gaaagccaaa
     4321 aaacccacaa cttcctcaaa tcgtcacttc cgttttccca cgttacgtca cttcccattt
     4381 taagaaaact acaattccca acacatacaa gttactccgc cctaaaacct acgtcacccg
     4441 ccccgttccc acgccccgcg ccacgtcaca aactccaccc cctcattatc atattggctt
     4501 caatccaaaa taaggtatat tattgatgat gttaattaac atgcatggat ccatatgcgg
     4561 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
     4621 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     4681 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     4741 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     4801 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     4861 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     4921 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     4981 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5041 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     5101 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     5161 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     5221 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     5281 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     5341 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     5401 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     5461 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     5521 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     5581 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     5641 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     5701 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     5761 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     5821 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagc catgagatta
     5881 tcaaaaagga tcttcaccta gatccttttc acgtagaaag ccagtccgca gaaacggtgc
     5941 tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag
     6001 agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg
     6061 acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc
     6121 aaagtaaact ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagctct
     6181 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
     6241 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
     6301 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
     6361 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
     6421 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
     6481 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
     6541 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
     6601 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
     6661 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
     6721 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     6781 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     6841 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     6901 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     6961 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaatttt gttaaaattt
     7021 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaccatcc cttataaatc
     7081 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     7141 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     7201 acgtgaacca tcaccctaat caagtttttt gtggtcgagg tgccgtaaag cactaaatcg
     7261 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     7321 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     7381 gctgcgcgta accaccacac ccgcgcgctt aatgcgccgc tacagggcgc gtccattcgc
     7441 cattcaggat cgaattaatt cttaattaa
//
