LOCUS       V014021                 7493 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014021
VERSION     V014021
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7493)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7493
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(22..402)
                     /direction=LEFT
                     /label="M13 ori"
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     repeat_region   440..542
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     misc_signal     630..780
                     /label="Ad5 Psi"
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        838..1141
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1142..1345
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     polyA_signal    1579..1700
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             1794..2213
                     /gene="IX"
                     /label="Hexon-interlacing protein"
                     /note="Hexon-interlacing protein from Human adenovirus C
                     serotype 5. Accession#: P03281"
     CDS             complement(2279..3613)
                     /gene="IVa2"
                     /label="Packaging protein 1"
                     /note="Packaging protein 1 from Human adenovirus C serotype
                     5. Accession#: P03271"
     CDS             complement(4198..4581)
                     /note="Early 4 ORF1 protein from Human adenovirus C
                     serotype 2. Accession#: P03242"
                     /label="Early 4 ORF1 protein"
     repeat_region   4891..4993
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     rep_origin      complement(5253..5841)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             6673..7464
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
       61 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      121 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      181 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      241 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      301 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      361 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggat
      421 cgaattaatt cttaattaac atcatcaata atatacctta ttttggattg aagccaatat
      481 gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt
      541 agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa cacatgtaag cgacggatgt
      601 ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg
      661 ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt aagatttggc cattttcgcg
      721 ggaaaactga ataagaggaa gtgaaatctg aataattttg tgttactcat agcgcgtaat
      781 actgtaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      841 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      901 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      961 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     1021 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     1081 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     1141 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     1201 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     1261 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     1321 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agagcagaat
     1381 tctatgctcg agccatgggg taccgtcgac gtagatctag cggccgccta agcttctaga
     1441 taagatatcc gatccaccgg atctagataa ctgatcataa tcagccatac cacatttgta
     1501 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
     1561 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     1621 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     1681 aaactcatca atgtatctta acgcggatct gggcgtggtt aagggtggga aagaatatat
     1741 aaggtggggg tcttatgtag ttttgtatct gttttgcagc agccgccgcc gccatgagca
     1801 ccaactcgtt tgatggaagc attgtgagct catatttgac aacgcgcatg cccccatggg
     1861 ccggggtgcg tcagaatgtg atgggctcca gcattgatgg tcgccccgtc ctgcccgcaa
     1921 actctactac cttgacctac gagaccgtgt ctggaacgcc gttggagact gcagcctccg
     1981 ccgccgcttc agccgctgca gccaccgccc gcgggattgt gactgacttt gctttcctga
     2041 gcccgcttgc aagcagtgca gcttcccgtt catccgcccg cgatgacaag ttgacggctc
     2101 ttttggcaca attggattct ttgacccggg aacttaatgt cgtttctcag cagctgttgg
     2161 atctgcgcca gcaggtttct gccctgaagg cttcctcccc tcccaatgcg gtttaaaaca
     2221 taaataaaaa accagactct gtttggattt ggatcaagca agtgtcttgc tgtctttatt
     2281 taggggtttt gcgcgcgcgg taggcccggg accagcggtc tcggtcgttg agggtcctgt
     2341 gtattttttc caggacgtgg taaaggtgac tctggatgtt cagatacatg ggcataagcc
     2401 cgtctctggg gtggaggtag caccactgca gagcttcatg ctgcggggtg gtgttgtaga
     2461 tgatccagtc gtagcaggag cgctgggcgt ggtgcctaaa aatgtctttc agtagcaagc
     2521 tgattgccag gggcaggccc ttggtgtaag tgtttacaaa gcggttaagc tgggatgggt
     2581 gcatacgtgg ggatatgaga tgcatcttgg actgtatttt taggttggct atgttcccag
     2641 ccatatccct ccggggattc atgttgtgca gaaccaccag cacagtgtat ccggtgcact
     2701 tgggaaattt gtcatgtagc ttagaaggaa atgcgtggaa gaacttggag acgcccttgt
     2761 gacctccaag attttccatg cattcgtcca taatgatggc aatgggccca cgggcggcgg
     2821 cctgggcgaa gatatttctg ggatcactaa cgtcatagtt gtgttccagg atgagatcgt
     2881 cataggccat ttttacaaag cgcgggcgga gggtgccaga ctgcggtata atggttccat
     2941 ccggcccagg ggcgtagtta ccctcacaga tttgcatttc ccacgctttg agttcagatg
     3001 gggggatcat gtctacctgc ggggcgatga agaaaacggt ttccggggta ggggagatca
     3061 gctgggaaga aagcaggttc ctgagcagct gcgacttacc gcagccggtg ggcccgtaaa
     3121 tcacacctat taccggctgc aactggtagt taagagagct gcagctgccg tcatccctga
     3181 gcaggggggc cacttcgtta agcatgtccc tgactcgcat gttttccctg accaaatccg
     3241 ccagaaggcg ctcgccgccc agcgatagca gttcttgcaa ggaagcaaag tttttcaacg
     3301 gtttgagacc gtccgccgta ggcatgcttt tgagcgtttg accaagcagt tccaggcggt
     3361 cccacagctc ggtcacctgc tctacggcat ctcgatccag catatctcct cgtttcgcgg
     3421 gttggggcgg ctttcgctgt acggcagtag tcggtgctcg tccagacggg ccagggtcat
     3481 gtctttccac gggcgcaggg tcctcgtcag cgtagtctgg gtcacggtga aggggtgcgc
     3541 tccgggctgc gcgctggcca gggtgcgctt gaggctggtc ctgctggtgc tgaagcgctg
     3601 ccggtcttcg ccctgcgcgt cggccaggta gcatttgacc atggtgtcat agtccagccc
     3661 ctccgcggcg tggcccttgg cgcgcagctt gcccttggag gaggcgccgc acgaggggca
     3721 gtgcagactt ttgagggcgt agagcttggg cgcgagaaat accgattccg gggagtaggc
     3781 atccgcgccg caggccccgc agacggtctc gcattccacg agccaggtga gctctggccg
     3841 ttcggggtca aaaaccaggt ttcccccatg ctttttgatg cgtttcttac ctctggtttc
     3901 catgagccgg tgtccacgct cggtgacgaa aaggctgtcc gtgtccccgt atacagactt
     3961 gagagggagt ttaaacgagt tcaatagctt gttgcatggg cggcgatata aaatgcaagg
     4021 tgctgctcaa aaaatcaggc aaagcctcgc gcaaaaaaga aagcacatcg tagtcatgct
     4081 catgcagata aaggcaggta agctccggaa ccaccacaga aaaagacacc atttttctct
     4141 caaacatgtc tgcgggtttc tgcataaaca caaaataaaa taacaaaaaa acatttaaac
     4201 attagaagcc tgtcttacaa caggaaaaac aacccttata agcataagac ggactacggc
     4261 catgccggcg tgaccgtaaa aaaactggtc accgtgatta aaaagcacca ccgacagctc
     4321 ctcggtcatg tccggagtca taatgtaaga ctcggtaaac acatcaggtt gattcacatc
     4381 ggtcagtgct aaaaagcgac cgaaatagcc cgggggaata catacccgca ggcgtagaga
     4441 caacattaca gcccccatag gaggtataac aaaattaata ggagagaaaa acacataaac
     4501 acctgaaaaa ccctcctgcc taggcaaaat agcaccctcc cgctccagaa caacatacag
     4561 cgcttccaca gcggcagcca taacagtcag ccttaccagt aaaaaagaaa acctattaaa
     4621 aaaacaccac tcgacacggc accagctcaa tcagtcacag tgtaaaaaag ggccaagtgc
     4681 agagcgagta tatataggac taaaaaatga cgtaacggtt aaagtccaca aaaaacaccc
     4741 agaaaaccgc acgcgaacct acgcccagaa acgaaagcca aaaaacccac aacttcctca
     4801 aatcgtcact tccgttttcc cacgttacgt cacttcccat tttaagaaaa ctacaattcc
     4861 caacacatac aagttactcc gccctaaaac ctacgtcacc cgccccgttc ccacgccccg
     4921 cgccacgtca caaactccac cccctcatta tcatattggc ttcaatccaa aataaggtat
     4981 attattgatg atgttaatta acatgcatgg atccatatgc ggtgtgaaat accgcacaga
     5041 tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg
     5101 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     5161 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     5221 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     5281 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     5341 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     5401 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     5461 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     5521 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     5581 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     5641 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     5701 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     5761 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     5821 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     5881 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     5941 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     6001 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     6061 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     6121 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     6181 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     6241 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     6301 agtttgcgca acgttgttgc cattgctgca gccatgagat tatcaaaaag gatcttcacc
     6361 tagatccttt tcacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc
     6421 agctactggg ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc
     6481 agtgggctta catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa
     6541 ttgccagctg gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct
     6601 ttcttgccgc caaggatctg atggcgcagg ggatcaagct ctgatcaaga gacaggatga
     6661 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     6721 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     6781 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     6841 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     6901 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     6961 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     7021 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     7081 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     7141 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     7201 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     7261 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     7321 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     7381 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     7441 tatcgccttc ttgacgagtt cttctgaatt ttgttaaaat ttttgttaaa tca
//
