LOCUS       V014333                 8211 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014333
VERSION     V014333
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8211)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(22..402)
                     /direction=LEFT
                     /label="M13 ori"
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     repeat_region   440..542
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     misc_signal     630..780
                     /label="Ad5 Psi"
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        838..1141
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1142..1345
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             1442..2158
                     /label="EGFP"
                     /note="enhanced GFP"
     polyA_signal    2297..2418
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             2512..2931
                     /gene="IX"
                     /label="Hexon-interlacing protein"
                     /note="Hexon-interlacing protein from Human adenovirus C
                     serotype 5. Accession#: P03281"
     CDS             complement(2997..4331)
                     /gene="IVa2"
                     /label="Packaging protein 1"
                     /note="Packaging protein 1 from Human adenovirus C serotype
                     5. Accession#: P03271"
     CDS             complement(4916..5299)
                     /note="Early 4 ORF1 protein from Human adenovirus C
                     serotype 2. Accession#: P03242"
                     /label="Early 4 ORF1 protein"
     repeat_region   5609..5711
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     rep_origin      complement(5971..6559)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             7391..8182
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
       61 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      121 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      181 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      241 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      301 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      361 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggat
      421 cgaattaatt cttaattaac atcatcaata atatacctta ttttggattg aagccaatat
      481 gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt
      541 agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa cacatgtaag cgacggatgt
      601 ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg
      661 ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt aagatttggc cattttcgcg
      721 ggaaaactga ataagaggaa gtgaaatctg aataattttg tgttactcat agcgcgtaat
      781 actgtaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
      841 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      901 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      961 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     1021 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     1081 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     1141 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     1201 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     1261 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     1321 gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct agagcagaat
     1381 tctatgctcg agccatgggg taccgtcgac gtagatctag cggccgccta agcttgccac
     1441 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     1501 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     1561 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     1621 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     1681 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     1741 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     1801 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     1861 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     1921 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     1981 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     2041 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     2101 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     2161 agatatccga tccaccggat ctagataact gatcataatc agccatacca catttgtaga
     2221 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     2281 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     2341 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     2401 actcatcaat gtatcttaac gcggatctgg gcgtggttaa gggtgggaaa gaatatataa
     2461 ggtgggggtc ttatgtagtt ttgtatctgt tttgcagcag ccgccgccgc catgagcacc
     2521 aactcgtttg atggaagcat tgtgagctca tatttgacaa cgcgcatgcc cccatgggcc
     2581 ggggtgcgtc agaatgtgat gggctccagc attgatggtc gccccgtcct gcccgcaaac
     2641 tctactacct tgacctacga gaccgtgtct ggaacgccgt tggagactgc agcctccgcc
     2701 gccgcttcag ccgctgcagc caccgcccgc gggattgtga ctgactttgc tttcctgagc
     2761 ccgcttgcaa gcagtgcagc ttcccgttca tccgcccgcg atgacaagtt gacggctctt
     2821 ttggcacaat tggattcttt gacccgggaa cttaatgtcg tttctcagca gctgttggat
     2881 ctgcgccagc aggtttctgc cctgaaggct tcctcccctc ccaatgcggt ttaaaacata
     2941 aataaaaaac cagactctgt ttggatttgg atcaagcaag tgtcttgctg tctttattta
     3001 ggggttttgc gcgcgcggta ggcccgggac cagcggtctc ggtcgttgag ggtcctgtgt
     3061 attttttcca ggacgtggta aaggtgactc tggatgttca gatacatggg cataagcccg
     3121 tctctggggt ggaggtagca ccactgcaga gcttcatgct gcggggtggt gttgtagatg
     3181 atccagtcgt agcaggagcg ctgggcgtgg tgcctaaaaa tgtctttcag tagcaagctg
     3241 attgccaggg gcaggccctt ggtgtaagtg tttacaaagc ggttaagctg ggatgggtgc
     3301 atacgtgggg atatgagatg catcttggac tgtattttta ggttggctat gttcccagcc
     3361 atatccctcc ggggattcat gttgtgcaga accaccagca cagtgtatcc ggtgcacttg
     3421 ggaaatttgt catgtagctt agaaggaaat gcgtggaaga acttggagac gcccttgtga
     3481 cctccaagat tttccatgca ttcgtccata atgatggcaa tgggcccacg ggcggcggcc
     3541 tgggcgaaga tatttctggg atcactaacg tcatagttgt gttccaggat gagatcgtca
     3601 taggccattt ttacaaagcg cgggcggagg gtgccagact gcggtataat ggttccatcc
     3661 ggcccagggg cgtagttacc ctcacagatt tgcatttccc acgctttgag ttcagatggg
     3721 gggatcatgt ctacctgcgg ggcgatgaag aaaacggttt ccggggtagg ggagatcagc
     3781 tgggaagaaa gcaggttcct gagcagctgc gacttaccgc agccggtggg cccgtaaatc
     3841 acacctatta ccggctgcaa ctggtagtta agagagctgc agctgccgtc atccctgagc
     3901 aggggggcca cttcgttaag catgtccctg actcgcatgt tttccctgac caaatccgcc
     3961 agaaggcgct cgccgcccag cgatagcagt tcttgcaagg aagcaaagtt tttcaacggt
     4021 ttgagaccgt ccgccgtagg catgcttttg agcgtttgac caagcagttc caggcggtcc
     4081 cacagctcgg tcacctgctc tacggcatct cgatccagca tatctcctcg tttcgcgggt
     4141 tggggcggct ttcgctgtac ggcagtagtc ggtgctcgtc cagacgggcc agggtcatgt
     4201 ctttccacgg gcgcagggtc ctcgtcagcg tagtctgggt cacggtgaag gggtgcgctc
     4261 cgggctgcgc gctggccagg gtgcgcttga ggctggtcct gctggtgctg aagcgctgcc
     4321 ggtcttcgcc ctgcgcgtcg gccaggtagc atttgaccat ggtgtcatag tccagcccct
     4381 ccgcggcgtg gcccttggcg cgcagcttgc ccttggagga ggcgccgcac gaggggcagt
     4441 gcagactttt gagggcgtag agcttgggcg cgagaaatac cgattccggg gagtaggcat
     4501 ccgcgccgca ggccccgcag acggtctcgc attccacgag ccaggtgagc tctggccgtt
     4561 cggggtcaaa aaccaggttt cccccatgct ttttgatgcg tttcttacct ctggtttcca
     4621 tgagccggtg tccacgctcg gtgacgaaaa ggctgtccgt gtccccgtat acagacttga
     4681 gagggagttt aaacgagttc aatagcttgt tgcatgggcg gcgatataaa atgcaaggtg
     4741 ctgctcaaaa aatcaggcaa agcctcgcgc aaaaaagaaa gcacatcgta gtcatgctca
     4801 tgcagataaa ggcaggtaag ctccggaacc accacagaaa aagacaccat ttttctctca
     4861 aacatgtctg cgggtttctg cataaacaca aaataaaata acaaaaaaac atttaaacat
     4921 tagaagcctg tcttacaaca ggaaaaacaa cccttataag cataagacgg actacggcca
     4981 tgccggcgtg accgtaaaaa aactggtcac cgtgattaaa aagcaccacc gacagctcct
     5041 cggtcatgtc cggagtcata atgtaagact cggtaaacac atcaggttga ttcacatcgg
     5101 tcagtgctaa aaagcgaccg aaatagcccg ggggaataca tacccgcagg cgtagagaca
     5161 acattacagc ccccatagga ggtataacaa aattaatagg agagaaaaac acataaacac
     5221 ctgaaaaacc ctcctgccta ggcaaaatag caccctcccg ctccagaaca acatacagcg
     5281 cttccacagc ggcagccata acagtcagcc ttaccagtaa aaaagaaaac ctattaaaaa
     5341 aacaccactc gacacggcac cagctcaatc agtcacagtg taaaaaaggg ccaagtgcag
     5401 agcgagtata tataggacta aaaaatgacg taacggttaa agtccacaaa aaacacccag
     5461 aaaaccgcac gcgaacctac gcccagaaac gaaagccaaa aaacccacaa cttcctcaaa
     5521 tcgtcacttc cgttttccca cgttacgtca cttcccattt taagaaaact acaattccca
     5581 acacatacaa gttactccgc cctaaaacct acgtcacccg ccccgttccc acgccccgcg
     5641 ccacgtcaca aactccaccc cctcattatc atattggctt caatccaaaa taaggtatat
     5701 tattgatgat gttaattaac atgcatggat ccatatgcgg tgtgaaatac cgcacagatg
     5761 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
     5821 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     5881 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     5941 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6001 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6061 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6121 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6181 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6241 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6301 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6361 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     6421 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6481 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     6541 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     6601 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     6661 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     6721 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     6781 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     6841 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     6901 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     6961 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     7021 tttgcgcaac gttgttgcca ttgctgcagc catgagatta tcaaaaagga tcttcaccta
     7081 gatccttttc acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag
     7141 ctactgggct atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag
     7201 tgggcttaca tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt
     7261 gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt
     7321 cttgccgcca aggatctgat ggcgcagggg atcaagctct gatcaagaga caggatgagg
     7381 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     7441 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     7501 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     7561 gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     7621 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     7681 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     7741 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     7801 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     7861 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag
     7921 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     7981 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     8041 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     8101 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     8161 tcgccttctt gacgagttct tctgaatttt gttaaaattt ttgttaaatc a
//
