LOCUS       pSecTag2_C.        5167 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian expression vector for the secretion of C-terminally 
            6xHis-tagged proteins. For other reading frames, use pSecTag2 A or 
            pSecTag2 B.
ACCESSION   .
VERSION     .
KEYWORDS    pSecTag2 C
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5167
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     sig_peptide     905..967
                     /label=Ig-kappa leader
                     /note="leader sequence from mouse immunoglobulin kappa 
                     light
                     chain"
     misc_feature    970..1089
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             1090..1119
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             1135..1152
                     /label=6xHis
                     /note="6xHis affinity tag"
     polyA_signal    1181..1405
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1451..1879
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1893..2222
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        2270..2317
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2336..2707
                     /label=BleoR
                     /note="antibiotic-binding protein"
     polyA_signal    2840..2973
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3010..3026)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3034..3050)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3058..3088)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3103..3124)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3412..4000)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4174..5031)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5032..5136)
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 caccatggag acagacacac tcctgctatg ggtactgctg ctctgggttc caggttccac
      961 tggtgacgcg gcccagccgg ccaggcgcgc gcgccgtacg tacgaagctt ggtaccgagc
     1021 tcggatccac tccagtgtgg tggaattctg cagatatcca gcacagtggc ggccgctcga
     1081 ggagggcccg aacaaaaact catctcagaa gaggatctga atagcgccgt cgaccatcat
     1141 catcatcatc attgagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     1201 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     1261 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     1321 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     1381 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     1441 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     1501 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     1561 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcggggcat ccctttaggg
     1621 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     1681 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     1741 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     1801 tttgatttat aagggatttt ggggatttcg gcctattggt taaaaaatga gctgatttaa
     1861 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     1921 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     1981 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     2041 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     2101 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     2161 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     2221 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta
     2281 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc
     2341 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt
     2401 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt
     2461 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac
     2521 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt
     2581 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg
     2641 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga
     2701 gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     2761 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     2821 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     2881 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     2941 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     3001 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     3061 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     3121 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     3181 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     3241 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     3301 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     3361 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     3421 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     3481 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     3541 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     3601 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     3661 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     3721 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     3781 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     3841 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     3901 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     3961 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     4021 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     4081 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     4141 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     4201 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     4261 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     4321 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     4381 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     4441 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     4501 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     4561 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     4621 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     4681 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     4741 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     4801 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     4861 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     4921 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     4981 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     5041 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     5101 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     5161 tgacgtc
//
