LOCUS       V003395                 5907 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003395
VERSION     V003395
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Woo HM.
  TITLE     Application of synthetic biology platform of CyanoBrick vectors:
            analysis of the elevated CO2 concentration on biomass and gene
            expression of Synechococcus elongatus PCC7942
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5907)
  AUTHORS   Woo HM.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAY-2014) Clean Energy Research Center, Korea
            Institute of Science and Technology, Hwarangno 14-gil 5,
            Seongbuk-gu, Seoul 136-791, Korea
REFERENCE   3  (bases 1 to 5907)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5907)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-MAY-2014) Clean Energy Research Center, Korea Institute of
            Science and Technology, Hwarangno 14-gil 5, Seongbuk-gu, Seoul
            136-791, Korea"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5907
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      84..672
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     gene            745..1543
                     /gene="NS1a"
                     /label="NS1a"
     CDS             1903..2691
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     promoter        2721..2798
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             2799..3878
                     /label="lacI"
                     /note="lac repressor"
     promoter        4113..4142
                     /label="trc promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    4150..4166
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             4207..4920
                     /label="GFPuv"
                     /note="GFP variant optimized for excitation by UV light"
     terminator      4956..5027
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      5043..5070
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     CDS             5423..5869
                     /gene="ndk"
                     /label="Nucleoside diphosphate kinase"
                     /note="Nucleoside diphosphate kinase from Synechococcus
                     elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805).
                     Accession#: P50590"
ORIGIN
        1 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
       61 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
      121 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
      181 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
      241 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
      301 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
      361 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
      421 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
      481 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
      541 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
      601 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
      661 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
      721 tttgctcaca tgtgtgctgg gccccaatgc cttctccaag ggcggcattc ccctgactgt
      781 tgaaggcgtt gccaatatca agattgctgg ggaagaaccg accatccaca acgcgatcga
      841 gcggctgctt ggcaaaaacc gtaaggaaat cgagcaaatt gccaaggaga ccctcgaagg
      901 caacttgcgt ggtgttttag ccagcctcac gccggagcag atcaacgagg acaaaattgc
      961 ctttgccaaa agtctgctgg aagaggcgga ggatgacctt gagcagctgg gtcaagtcct
     1021 cgatacgctg caagtccaga acatttccga tgaggtcggt tatctctcgg ctagtggacg
     1081 caagcagcgg gctgatctgc agcgagatgc ccgaattgct gaagccgatg cccaggctgc
     1141 ctctgcgatc caaacggccg aaaatgacaa gatcacggcc ctgcgtcgga tcgatcgcga
     1201 tgtagcgatc gcccaagccg aggccgagcg ccggattcag gatgcgttga cgcggcgcga
     1261 agcggtggtg gccgaagctg aagcggacat tgctaccgaa gtcgctcgta gccaagcaga
     1321 actccctgtg cagcaggagc ggatcaaaca ggtgcagcag caacttcaag ccgatgtgat
     1381 cgccccagct gaggcagctt gtaaacgggc gatcgcggaa gcgcgggggg ccgccgcccg
     1441 tatcgtcgaa gatggaaaag ctcaagcgga agggacccaa cggctggcgg aggcttggca
     1501 gaccgctggt gctaatgccc gcgacatctt cctgctccag aagtctagac cagccaggac
     1561 agaaatgcct cgacttcgct gctacccaag gttgccgggt gacgcacacc gtggaaacgg
     1621 atgaaggcac gaacccagtg gacataagcc tgttcggttc gtaagctgta atgcaagtag
     1681 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
     1741 gtggcggttt tcatggcttg ttatgactgt ttttttgggg tacagtctat gcctcgggca
     1801 tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat
     1861 gttacgcagc agggcagtcg ccctaaaaca aagttaaaca ttatgaggga agcggtgatc
     1921 gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca tctcgaaccg
     1981 acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa gccacacagt
     2041 gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg gcgagctttg
     2101 atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct ccgcgctgta
     2161 gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc taagcgcgaa
     2221 ctgcaatttg gagaatggca gcgcaatgac attcttgctg gtatcttcga gccagccacg
     2281 atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt tgccttggta
     2341 ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt tgaggcgcta
     2401 aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga gcgaaatgta
     2461 gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc gccgaaggat
     2521 gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt catacttgaa
     2581 gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc agatcagttg
     2641 gaagaatttg tccactacgt gaaaggcgag atcaccaagg tagtcggcaa ataacctcat
     2701 tttcgccaga tatcgacgtc gacaccatcg aatggtgcaa aacctttcgc ggtatggcat
     2761 gatagcgccc ggaagagagt caattcaggg tggtgaatgt gaaaccagta acgttatacg
     2821 atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca
     2881 gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca
     2941 ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca
     3001 cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg
     3061 atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta
     3121 aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc
     3181 tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc
     3241 ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc
     3301 gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc
     3361 cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca
     3421 atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac
     3481 aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc
     3541 agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata
     3601 tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca
     3661 ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct
     3721 ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa
     3781 ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     3841 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgta
     3901 agttagcgcg aattgatctg gtttgacagc ttatcatcga ctgcacggtg caccaatgct
     3961 tctggcgtca ggcagccatc ggaagctgtg gtatggctgt gcaggtcgta aatcactgca
     4021 taattcgtgt cgctcaaggc gcactcccgt tctggataat gttttttgcg ccgacatcat
     4081 aacggttctg gcaaatattc tgaaatgagc tgttgacaat taatcatccg gctcgtataa
     4141 tgtgtggaat tgtgagcgga taacaatttc agaattcaaa agatctttta agaaggagat
     4201 atacatatga gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta
     4261 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca
     4321 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccgtggcca
     4381 acacttgtca ctactttctc ttatggtgtt caatgctttt cccgttatcc ggatcacatg
     4441 aaacggcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga acgcactata
     4501 tctttcaaag atgacgggaa ctacaagacg cgtgctgaag tcaagtttga aggtgatacc
     4561 cttgttaatc gtatcgagtt aaaaggtatt gattttaaag aagatggaaa cattctcgga
     4621 cacaaactgg agtacaacta taactcacac aatgtataca tcacggcaga caaacaaaag
     4681 aatggaatca aagctaactt caaaattcgc cacaacattg aagatggctc cgttcaacta
     4741 gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt accagacaac
     4801 cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagcg tgaccacatg
     4861 gtccttcttg agtttgtaac tgctgctggg attacacatg gcatggatga gctctacaaa
     4921 taaggatcca aactcgagta aggatctcca ggcatcaaat aaaacgaaag gctcagtcga
     4981 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     5041 ctggctcacc ttcgggtggg cctttctgcg tttataccta gggcgttcgg ctgcggcgag
     5101 cggtatcagc tcactcaaag gcggtaatac gtccctgctc gtcacgcttt caggcaccgt
     5161 gccagatatc gacgtggagt cgatcactgt gattggcgaa ggggaaggca gcgctaccca
     5221 aatcgctagc ttgctggaga agctgaaaca aaccacgggc attgatctgg cgaaatccct
     5281 accgggtcaa tccgactcgc ccgctgcgaa gtcctaagag atagcgatgt gaccgcgatc
     5341 gcttgtcaag aatcccagtg atcccgaacc ataggaaggc aagctcaatg cttgcctcgt
     5401 cttgaggact atctagatgt ctgtggaacg cacatttatt gccatcaagc ccgatggcgt
     5461 tcagcggggt ttggtcggta cgatcatcgg ccgctttgag caaaaaggct tcaaactggt
     5521 gggcctaaag cagctgaagc ccagtcgcga gctggccgaa cagcactatg ctgtccaccg
     5581 cgagcgcccc ttcttcaatg gcctcgtcga gttcatcacc tctgggccga tcgtggcgat
     5641 cgtcttggaa ggcgaaggcg ttgtggcggc tgctcgcaag ttgatcggcg ctaccaatcc
     5701 gctgacggca gaaccgggca ccatccgtgg tgattttggt gtcaatattg gccgcaacat
     5761 catccatggc tcggatgcaa tcgaaacagc acaacaggaa attgctctct ggtttagccc
     5821 agcagagcta agtgattgga cccccacgat tcaaccctgg ctgtacgaat aaggtctgca
     5881 ttccttcaga gagacattgc catgccg
//
