LOCUS       V003430                10819 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003430
VERSION     V003430
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10819)
  AUTHORS   Nafisi PM, Aksel T, Douglas SM.
  TITLE     Construction of a novel phagemid to produce custom DNA origami
            scaffolds
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10819)
  AUTHORS   Nafisi PM, Aksel T, Douglas SM.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2018) Cellular and Molecular Pharmacology,
            University of California, San Francisco, 600 16th Street, Genentech
            Hall S-474, San Francisco, CA 94158, USA
REFERENCE   3  (bases 1 to 10819)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10819)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-MAY-2018) Cellular and Molecular Pharmacology, University of
            California, San Francisco, 600 16th Street, Genentech Hall S-474,
            San Francisco, CA 94158, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      44..424
                     /label="M13 ori"
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     misc_feature    425..2992
                     /label="Insert G"
                     /note="Insert G"
     CDS             3109..3846
                     /gene="V"
                     /label="Tail tube protein"
                     /note="Tail tube protein from Escherichia phage lambda.
                     Accession#: P03733"
     CDS             3865..4284
                     /gene="G"
                     /label="Tail assembly protein G"
                     /note="Tail assembly protein G from Escherichia phage
                     lambda. Accession#: P03734"
     CDS             6518..6913
                     /gene="Fi"
                     /label="DNA-packaging protein FI"
                     /note="DNA-packaging protein FI from Escherichia phage
                     lambda. Accession#: P03709"
     CDS             6928..7278
                     /gene="FII"
                     /label="Head-tail connector protein FII"
                     /note="Head-tail connector protein FII from Escherichia
                     phage lambda. Accession#: P03714"
     rep_origin      8108..8437
                     /note="M13term; M13 origin modified with 3-base mutation to
                     eliminate initation capabilities, resulting in M13
                     terminator"
     misc_feature    8388..8392
                     /label="term-alpha"
                     /note="term-alpha"
     misc_feature    8406..8410
                     /label="term-beta"
                     /note="term-beta"
     misc_feature    8415..8420
                     /label="term-gamma"
                     /note="term-gamma"
     misc_feature    8424..8429
                     /note="term-delta-T3; TGGAAC mutated to TGGTTT"
     primer_bind     complement(8453..8469)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    8477..8493
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8501..8531)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8546..8567)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8855..9443)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9617..10474)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10475..10579)
                     /label="AmpR promoter"
ORIGIN
        1 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgacgcgcc ctgtagcggc
       61 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
      121 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
      181 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
      241 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
      301 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
      361 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
      421 tcggggtacc ttttttagat ctctgttaca cactgttccg ggatgcttat catctggcgg
      481 ggattgagat gccggacttt catcgtgagg atgactggtg gcgtaacggc cagaatctct
      541 atctggataa tctggaggcg acggggctgt atcaggtgcc gttgtcagcg gcacagccgg
      601 gcgatgtgct gctgtgctgt tttggttcat cagtgccgaa tcacgccgca atttactgcg
      661 gcgacggcga gctgctgcac catattcctg aacaactgag caaacgagag aggtacaccg
      721 acaaatggca gcgacgcaca cactccctct ggcgtcaccg ggcatggcgc gcatctgcct
      781 ttacggggat ttacaacgat ttggtcgccg catcgacctt cgtgtgaaaa cgggggctga
      841 agccatccgg gcactggcca cacagctccc ggcgtttcgt cagaaactga gcgacggctg
      901 gtatcaggta cggattgccg ggcgggacgt cagcacgtcc gggttaacgg cgcagttaca
      961 tgagactctg cctgatggcg ctgtaattca tattgttccc agagtcgccg gggccaagtc
     1021 aggtggcgta ttccagattg tcctgggggc tgccgccatt gccggatcat tctttaccgc
     1081 cggagccacc cttgcagcat ggggggcagc cattggggcc ggtggtatga ccggcatcct
     1141 gttttctctc ggtgccagta tggtgctcgg tggtgtggcg cagatgctgg caccgaaagc
     1201 cagaactccc cgtatacaga caacggataa cggtaagcag aacacctatt tctcctcact
     1261 ggataacatg gttgcccagg gcaatgttct gcctgttctg tacggggaaa tgcgcgtggg
     1321 gtcacgcgtg gtttctcagg agatcagcac ggcagacgaa ggggacggtg gtcaggttgt
     1381 ggtgattggt cgctgatgca aaatgtttta tgtgaaaccg cctgcgggcg gttttgtcat
     1441 ttatggagcg tgaggaatgg gtaaaggaag cagtaagggg cataccccgc gcgaagcgaa
     1501 ggacaacctg aagtccacgc agttgctgag tgtgatcgat gccatcagcg aagggccgat
     1561 tgaaggtccg gtggatggct taaaaagcgt gctgctgaac agtacgccgg tgctggacac
     1621 tgaggggaat accaacatat ccggtgtcac ggtggtgttc cgggctggtg agcaggagca
     1681 gactccgccg gagggatttg aatcctccgg ctccgagacg gtgctgggta cggaagtgaa
     1741 atatgacacg ccgatcaccc gcaccattac gtctgcaaac atcgaccgtc tgcgctttac
     1801 cttcggtgta caggcactgg tggaaaccac ctcaaagggt gacaggaatc cgtcggaagt
     1861 ccgcctgctg gttcagatac aacgtaacgg tggctgggtg acggaaaaag acatcaccat
     1921 taagggcaaa accacctcgc agtatctggc ctcggtggtg atgggtaacc tgccgccgcg
     1981 cccgtttaat atccggatgc gcaggatgac gccggacagc accacagacc agctgcagaa
     2041 caaaacgctc tggtcgtcat acactgaaat catcgatgtg aaacagtgct acccgaacac
     2101 ggcactggtc ggcgtgcagg tggactcgga gcagttcggc agccagcagg tgagccgtaa
     2161 ttatcatctg cgcgggcgta ttctgcaggt gccgtcgaac tataacccgc agacgcggca
     2221 atacagcggt atctgggacg gaacgtttaa accggcatac agcaacaaca tggcctggtg
     2281 tctgtgggat atgctgaccc atccgcgcta cggcatgggg aaacgtcttg gtgcggcgga
     2341 tgtggataaa tgggcgctgt atgtcatcgg ccagtactgc gaccagtcag tgccggacgg
     2401 ctttggcggc acggagccgc gcatcacctg taatgcgtac ctgaccacac agcgtaaggc
     2461 gtgggatgtg ctcagcgatt tctgctcggc gatgcgctgt atgccggtat ggaacgggca
     2521 gacgctgacg ttcgtgcagg accgaccgtc ggataagacg tggacctata accgcagtaa
     2581 tgtggtgatg ccggatgatg gcgcgccgtt ccgctacagc ttcagcgccc tgaaggaccg
     2641 ccataatgcc gttgaggtga actggattga cccgaacaac ggctgggaga cggcgacaga
     2701 gcttgttgaa gatacgcagg ccattgcccg ttacggtcgt aatgttacga agatggatgc
     2761 ctttggctgt accagccggg ggcaggcaca ccgcgccggg ctgtggctga ttaaaacaga
     2821 actgctggaa acgcagaccg tggatttcag cgtcggcgca gaagggcttc gccatgtacc
     2881 gggcgatgtt attgaaatct gcgatgatga ctatgccggt atcagcaccg gtggtcgtgt
     2941 gctggcggtg aacagccaga cccggacgct gacgctcgac cgtgaaatca cggatcttga
     3001 tcaccagtat ggtggccagc ggctatgact accggcgcga cgatgatgcg ggcttgtgga
     3061 gttcagccga tctgacttat gtcattacct atgaaatgtg aggacgctat gcctgtacca
     3121 aatcctacaa tgccggtgaa aggtgccggg accaccctgt gggtttataa ggggagcggt
     3181 gacccttacg cgaatccgct ttcagacgtt gactggtcgc gtctggcaaa agttaaagac
     3241 ctgacgcccg gcgaactgac cgctgagtcc tatgacgaca gctatctcga tgatgaagat
     3301 gcagactgga ctgcgaccgg gcaggggcag aaatctgccg gagataccag cttcacgctg
     3361 gcgtggatgc ccggagagca ggggcagcag gcgctgctgg cgtggtttaa tgaaggcgat
     3421 acccgtgcct ataaaatccg cttcccgaac ggcacggtcg atgtgttccg tggctgggtc
     3481 agcagtatcg gtaaggcggt gacggcgaag gaagtgatca cccgcacggt gaaagtcacc
     3541 aatgtgggac gtccgtcgat ggcagaagat cgcagcacgg taacagcggc aaccggcatg
     3601 accgtgacgc ctgccagcac ctcggtggtg aaagggcaga gcaccacgct gaccgtggcc
     3661 ttccagccgg agggcgtaac cgacaagagc tttcgtgcgg tgtctgcgga taaaacaaaa
     3721 gccaccgtgt cggtcagtgg tatgaccatc accgtgaacg gcgttgctgc aggcaaggtc
     3781 aacattccgg ttgtatccgg taatggtgag tttgctgcgg ttgcagaaat taccgtcacc
     3841 gccagttaat ccggagagtc agcgatgttc ctgaaaaccg aatcatttga acataacggt
     3901 gtgaccgtca cgctttctga actgtcagcc ctgcagcgca ttgagcatct cgccctgatg
     3961 aaacggcagg cagaacaggc ggagtcagac agcaaccgga agtttactgt ggaagacgcc
     4021 atcagaaccg gcgcgtttct ggtggcgatg tccctgtggc ataaccatcc gcagaagacg
     4081 cagatgccgt ccatgaatga agccgttaaa cagattgagc aggaagtgct taccacctgg
     4141 cccacggagg caatttctca tgctgaaaac gtggtgtacc ggctgtctgg tatgtatgag
     4201 tttgtggtga ataatgcccc tgaacagaca gaggacgccg ggcccgcaga gcctgtttct
     4261 gcgggaaagt gttcgacggt gagctgagtt ttgccctgaa actggcgcgt gagatggggc
     4321 gacccgactg gcgtgccatg cttgccggga tgtcatccac ggagtatgcc gactggcacc
     4381 gcttttacag tacccattat tttcatgatg ttctgctgga tatgcacttt tccgggctga
     4441 cgtacaccgt gctcagcctg tttttcagcg atccggatat gcatccgctg gatttcagtc
     4501 tgctgaaccg gcgcgaggct gacgaagagc ctgaagatga tgtgctgatg cagaaagcgg
     4561 cagggcttgc cggaggtgtc cgctttggcc cggacgggaa tgaagttatc cccgcttccc
     4621 cggatgtggc ggacatgacg gaggatgacg taatgctgat gacagtatca gaagggatcg
     4681 caggaggagt ccggtatggc tgaaccggta ggcgatctgg tcgttgattt gagtctggat
     4741 gcggccagat ttgacgagca gatggccaga gtcaggcgtc atttttctgg tacggaaagt
     4801 gatgcgaaaa aaacagcggc agtcgttgaa cagtcgctga gccgacaggc gctggctgca
     4861 cagaaagcgg ggatttccgt cgggcagtat aaagccgcca tgcgtatgct gcctgcacag
     4921 ttcaccgacg tggccacgca gcttgcaggc gggcaaagtc cgtggctgat cctgctgcaa
     4981 cagggggggc aggtgaagga ctccttcggc gggatgatcc ccatgttcag ggggcttgcc
     5041 ggtgcgatca ccctgccgat ggtgggggcc acctcgctgg cggtggcgac cggtgcgctg
     5101 gcgtatgcct ggtatcaggg caactcaacc ctgtccgatt tcaacaaaac gctggtcctt
     5161 tccggcaatc aggcgggact gacggcagat cgtatgctgg tcctgtccag agccgggcag
     5221 gcggcagggc tgacgtttaa ccagaccagc gagtcactca gcgcactggt taaggcgggg
     5281 gtaagcggtg aggctcagat tgcgtccatc agccagagtg tggcgcgttt ctcctctgca
     5341 tccggcgtgg aggtggacaa ggtcgctgaa gccttcggga agctgaccac agacccgacg
     5401 tcggggctga cggcgatggc tcgccagttc cataacgtgt cggcggagca gattgcgtat
     5461 gttgctcagt tgcagcgttc cggcgatgaa gccggggcat tgcaggcggc gaacgaggcc
     5521 gcaacgaaag ggtttgatga ccagacggat ctaccacgga gaaagtctat ctctcacaaa
     5581 ttccgggact ggtaaacatg gcgctgtacg tttcgccgat tgtttccggt gaggttatcc
     5641 gttcccgtgg cggctccacc tctgaattta cgccgggata tgtcaagccg aagcatgaag
     5701 tgaatccgca gatgaccctg cgtcgcctgc cggatgaaga tccgcagaat ctggcggacc
     5761 cggcttaccg ccgccgtcgc atcatcatgc agaacatgcg tgacgaagag ctggccattg
     5821 ctcaggtcga agagatgcag gcagtttctg ccgtgcttaa gggcaaatac accatgaccg
     5881 gtgaagcctt cgatccggtt gaggtggata tgggccgcag tgaggagaat aacatcacgc
     5941 agtccggcgg cacggagtgg agcaagcgtg acaagtccac gtatgacccg accgacgata
     6001 tcgaagccta cgcgctgaac gccagcggtg tggtgaatat catcgtgttc gatccgaaag
     6061 gctgggcgct gttccgttcc ttcaaagccg tcaaggagaa gctggatacc cgtcgtggct
     6121 ctaattccga gctggagaca gcggtgaaag acctgggcaa agcggtgtcc tataagggga
     6181 tgtatggcga tgtggccatc gtcgtgtatt ccggacagta cgtggaaaac ggcgtcaaaa
     6241 agaacttcct gccggacaac acgatggtgc tggggaacac tcaggcacgc ggtctgcgca
     6301 cctatggctg cattcaggat gcggacgcac agcgcgaagg cattaacgcc tctgcccgtt
     6361 acccgaaaaa ctgggtgacc accggcgatc cggcgcgtga gttcaccatg attcagtcag
     6421 caccgctgat gctgctggct gaccctgatg agttcgtgtc cgtacaactg gcgtaatcat
     6481 ggcccttcgg ggccattgtt tctctgtgga ggagtccatg acgaaagatg aactgattgc
     6541 ccgtctccgc tcgctgggtg aacaactgaa ccgtgatgtc agcctgacgg ggacgaaaga
     6601 agaactggcg ctccgtgtgg cagagctgaa agaggagctt gatgacacgg atgaaactgc
     6661 cggtcaggac acccctctca gccgggaaaa tgtgctgacc ggacatgaaa atgaggtggg
     6721 atcagcgcag ccggataccg tgattctgga tacgtctgaa ctggtcacgg tcgtggcact
     6781 ggtgaagctg catactgatg cacttcacgc cacgcgggat gaacctgtgg catttgtgct
     6841 gccgggaacg gcgtttcgtg tctctgccgg tgtggcagcc gaaatgacag agcgcggcct
     6901 ggccagaatg caataacggg aggcgctgtg gctgatttcg ataacctgtt cgatgctgcc
     6961 attgcccgcg ccgatgaaac gatacgcggg tacatgggaa cgtcagccac cattacatcc
     7021 ggtgagcagt caggtgcggt gatacgtggt gtttttgatg accctgaaaa tatcagctat
     7081 gccggacagg gcgtgcgcgt tgaaggctcc agcccgtccc tgtttgtccg gactgatgag
     7141 gtgcggcagc tgcggcgtgg agacacgctg accatcggtg aggaaaattt ctgggtagat
     7201 cgggtttcgc cggatgatgg cggaagttgt catctctggc ttggacgggg cgtaccgcct
     7261 gccgttaacc gtcgccgctg aaagggggat gtatggccat aaaaggtctt gagcaggccg
     7321 ttgaaaacct cagccgtatc agcaaaacgg cggtgcctgg tgccgccgca atggccatta
     7381 accgcgttgc ttcatccgcg atatcgcagt cggcgtcaca ggttgcccgt gagacaaagg
     7441 tacgccggaa actggtaaag gaaagggcca ggctgaaaag ggccacggtc aaaaatccgc
     7501 aggccagaat caaagttaac cggggggatt tgcccgtaat caagctgggt aatgcgcggg
     7561 ttgtcctttc gcgccgcagg cgtcgtaaaa aggggcagcg ttcatccctg aaaggtggcg
     7621 gcagcgtgct tgtggtgggt aaccgtcgta ttcccggcgc gtttattcag caactgaaaa
     7681 atggccggtg gcatgtcatg cagcgtgtgg ctgggaaaaa ccgttacccc attgatgtgg
     7741 tgaaaatccc gatggcggtg ccgctgacca cggcgtttaa acaaaatatt gagcggatac
     7801 ggcgtgaacg tcttccgaaa gagctgggct atgcgctgca gcatcaactg aggatggtaa
     7861 taaagcgatg aaacatactg aactccgtgc agccgtactg gatgcactgg agaagcatga
     7921 caccggggcg acgttttttg atggtcgccc cgctgttttt gatgaggcgg attttccggc
     7981 agttgccgtt tatctcaccg gcgctgaata cacgggcgaa gagctggaca gcgatacctg
     8041 gcaggcggag ctgcatatcg aagttttcct gcctgctcag gtgccggatt cagagctgga
     8101 tggatccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     8161 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     8221 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     8281 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
     8341 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     8401 aatagtggac tcttgttcca aactggttta acactcagaa ttcgtaatca tggtcatagc
     8461 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     8521 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     8581 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     8641 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     8701 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     8761 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     8821 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     8881 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     8941 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     9001 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     9061 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     9121 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     9181 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     9241 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     9301 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     9361 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     9421 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     9481 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     9541 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     9601 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     9661 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     9721 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     9781 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     9841 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     9901 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     9961 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    10021 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    10081 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    10141 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    10201 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    10261 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    10321 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    10381 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    10441 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    10501 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    10561 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
    10621 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
    10681 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
    10741 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
    10801 ggtgtcgggg ctggcttaa
//
