LOCUS       V003433                10315 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003433
VERSION     V003433
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10315)
  AUTHORS   Nafisi PM, Aksel T, Douglas SM.
  TITLE     Construction of a novel phagemid to produce custom DNA origami
            scaffolds
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10315)
  AUTHORS   Nafisi PM, Aksel T, Douglas SM.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2018) Cellular and Molecular Pharmacology,
            University of California, San Francisco, 600 16th Street, Genentech
            Hall S-474, San Francisco, CA 94158, USA
REFERENCE   3  (bases 1 to 10315)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10315)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-MAY-2018) Cellular and Molecular Pharmacology, University of
            California, San Francisco, 600 16th Street, Genentech Hall S-474,
            San Francisco, CA 94158, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10315
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      44..424
                     /label="M13 ori"
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(2611..3207)
                     /gene="K"
                     /label="Tail tip assembly protein K"
                     /note="Tail tip assembly protein K from Escherichia phage
                     lambda. Accession#: P03729"
     CDS             complement(3359..4054)
                     /gene="L"
                     /label="Tail tip protein L"
                     /note="Tail tip protein L from Escherichia phage lambda.
                     Accession#: P03738"
     CDS             complement(4383..6941)
                     /gene="H"
                     /label="Tape measure protein"
                     /note="Tape measure protein from Escherichia phage lambda.
                     Accession#: P03736"
     rep_origin      7604..7933
                     /note="M13term; M13 origin modified with 3-base mutation to
                     eliminate initation capabilities, resulting in M13
                     terminator"
     misc_feature    7884..7888
                     /label="term-alpha"
                     /note="term-alpha"
     misc_feature    7902..7906
                     /label="term-beta"
                     /note="term-beta"
     misc_feature    7911..7916
                     /label="term-gamma"
                     /note="term-gamma"
     misc_feature    7920..7925
                     /note="term-delta-T3; TGGAAC mutated to TGGTTT"
     primer_bind     complement(7949..7965)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    7973..7989
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7997..8027)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8042..8063)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8351..8939)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9113..9970)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9971..10075)
                     /label="AmpR promoter"
ORIGIN
        1 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgacgcgcc ctgtagcggc
       61 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
      121 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
      181 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
      241 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
      301 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
      361 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
      421 tcggggtacc ggaggatggc agcgtgattt cacggtcgag cgtcagcgtc cgggtctggc
      481 tgttcaccgc cagcacacga ccaccggtgc tgataccggc atagtcatca tcgcagattt
      541 caataacatc gcccggtaca tggcgaagcc cttctgcgcc gacgctgaaa tccacggtct
      601 gcgtttccag cagttctgtt ttaatcagcc acagcccggc gcggtgtgcc tgcccccggc
      661 tggtacagcc aaaggcatcc atcttcgtaa cattacgacc gtaacgggca atggcctgcg
      721 tatcttcaac aagctctgtc gccgtctccc agccgttgtt cgggtcaatc cagttcacct
      781 caacggcatt atggcggtcc ttcagggcgc tgaagctgta gcggaacggc gcgccatcat
      841 ccggcatcac cacattactg cggttatagg tccacgtctt atccgacggt cggtcctgca
      901 cgaacgtcag cgtctgcccg ttccataccg gcatacagcg catcgccgag cagaaatcgc
      961 tgagcacatc ccacgcctta cgctgtgtgg tcaggtacgc attacaggtg atgcgcggct
     1021 ccgtgccgcc aaagccgtcc ggcactgact ggtcgcagta ctggccgatg acatacagcg
     1081 cccatttatc cacatccgcc gcaccaagac gtttccccat gccgtagcgc ggatgggtca
     1141 gcatatccca cagacaccag gccatgttgt tgctgtatgc cggtttaaac gttccgtccc
     1201 agataccgct gtattgccgc gtctgcgggt tatagttcga cggcacctgc agaatacgcc
     1261 cgcgcagatg ataattacgg ctcacctgct ggctgccgaa ctgctccgag tccacctgca
     1321 cgccgaccag tgccgtgttc gggtagcact gtttcacatc gatgatttca gtgtatgacg
     1381 accagagcgt tttgttctgc agctggtctg tggtgctgtc cggcgtcatc ctgcgcatcc
     1441 ggatattaaa cgggcgcggc ggcaggttac ccatcaccac cgaggccaga tactgcgagg
     1501 tggttttgcc cttaatggtg atgtcttttt ccgtcaccca gccaccgtta cgttgtatct
     1561 gaaccagcag gcggacttcc gacggattcc tgtcaccctt tgaggtggtt tccaccagtg
     1621 cctgtacacc gaaggtaaag cgcagacggt cgatgtttgc agacgtaatg gtgcgggtga
     1681 tcggcgtgtc atatttcact tccgtaccca gcaccgtctc ggagccggag gattcaaatc
     1741 cctccggcgg agtctgctcc tgctcaccag cccggaacac caccgtgaca ccggatatgt
     1801 tggtattccc ctcagtgtcc agcaccggcg tactgttcag cagcacgctt tttaagccat
     1861 ccaccggacc ttcaatcggc ccttcgctga tggcatcgat cacactcagc aactgcgtgg
     1921 acttcaggtt gtccttcgct tcgcgcgggg tatgcccctt actgcttcct ttacccattc
     1981 ctcacgctcc ataaatgaca aaaccgcccg caggcggttt cacataaaac attttgcatc
     2041 agcgaccaat caccacaacc tgaccaccgt ccccttcgtc tgccgtgctg atctcctgag
     2101 aaaccacgcg tgaccccacg cgcatttccc cgtacagaac aggcagaaca ttgccctggg
     2161 caaccatgtt atccagtgag gagaaatagg tgttctgctt accgttatcc gttgtctgta
     2221 tacggggagt tctggctttc ggtgccagca tctgcgccac accaccgagc accatactgg
     2281 caccgagaga aaacaggatg ccggtcatac caccggcccc aatggctgcc ccccatgctg
     2341 caagggtggc tccggcggta aagaatgatc cggcaatggc ggcagccccc aggacaatct
     2401 ggaatacgcc acctgacttg gccccggcga ctctgggaac aatatgaatt acagcgccat
     2461 caggcagagt ctcatgtaac tgcgccgtta acccggacgt gctgacgtcc cgcccggcaa
     2521 tccgtacctg ataccagccg tcgctcagtt tctgacgaaa cgccgggagc tgtgtggcca
     2581 gtgcccggat ggcttcagcc cccgttttca cacgaaggtc gatgcggcga ccaaatcgtt
     2641 gtaaatcccc gtaaaggcag atgcgcgcca tgcccggtga cgccagaggg agtgtgtgcg
     2701 tcgctgccat ttgtcggtgt acctctctcg tttgctcagt tgttcaggaa tatggtgcag
     2761 cagctcgccg tcgccgcagt aaattgcggc gtgattcggc actgatgaac caaaacagca
     2821 cagcagcaca tcgcccggct gtgccgctga caacggcacc tgatacagcc ccgtcgcctc
     2881 cagattatcc agatagagat tctggccgtt acgccaccag tcatcctcac gatgaaagtc
     2941 cggcatctca atccccgcca gatgataagc atcccggaac agtgtgtaac agtccgtcac
     3001 accgtgctca aagcgccgcc cggtgagatg cggcacacag cggaacttat gaatcgtccc
     3061 ccggcagacc agccaccacg gcaaatcact ctgcacctgc agccgccggt cggcctcact
     3121 cagccagggc agaccaccgg ggtggctgtg gaccagcgcc acaatctcac cctgcatttc
     3181 tgcctgcagc cagtcttccg gcgacatacg gaaatagcct ccggctcacc ggagatattc
     3241 acgcagggga aatatctttc cccctccggc gtgcttacca cgaagccgca cgactccgct
     3301 ggcgcacatc gccgggcgtg cgccagaatc gctgattctg tctgtgtcat gggatttact
     3361 gcgaaagttt gttaatggaa aggaagccgc caaagttgcc gacgttattg cggaacttac
     3421 aaccgctcag gcatttgctg catttatcct tcgtgatatc ggacgttggc tggtcatatt
     3481 catccgcgac agccggaccg ctataaccgc actcgtcacc gcgataggtc caggtgcagg
     3541 tgttggccag catgatacgt cccggaaaaa cagcgccatc cgtttccgtc ggcgtggaca
     3601 gtacaaagga ggcactcacc gcgctcagtt cgctgcactg ctcaatgcgc cagcggctga
     3661 tcacctcctg ctccggatcg gcgtaactgt ttccgttgac gaagttcacc gcatccagaa
     3721 aacgggcgta aaccttacgc cggaccaccg ttccgccgac cagactctgc atatcttccg
     3781 ccatcccggt gaccataccg tacaggttag aaaccgtcag cgtggggcgc gtactggtgc
     3841 ctttgccatt cagttcaaaa ccgctcccct gaatgggata cggctgatac tgtcgcccct
     3901 gccaggtgac cggctcacct ttttcgttct gctcattaca gaaaaaataa cgttctccac
     3961 cgacctctgt caggtcgatt tcccagagca ccacgctggc cgactgctcc gcacgggtgc
     4021 attcattcag tgtttcctgc cggatatcct gcatcagttc accacctgtt caaactctgc
     4081 gctgaactca acacgcagca tactgacccg cgacgaccat tttgcgcagg tcacctttat
     4141 ctgccgccac tcataaggcg gcgtccacag aaaggatttc cagcccccgt gctcttccag
     4201 aaacgactcc agtaccgtgg cctcctcacg ggggacagaa agcgtcacgc tgtacgtttt
     4261 caggttggca ttcagcccgg caggcgctcg ctgagaatag ccatcaccaa agcgcacctt
     4321 tcttacagaa gggaccgaag ccacatccat accgggtttc actttccagc ggaaggtctt
     4381 catcgtccac ctccggagaa caggccacca tcacgcatct gtgtctgaat ttcatcacgg
     4441 gcacccttgc gggccatgtc atacaccgcc ttcagagcag ccggacctat ctgcccgttc
     4501 gtgccgtcgt tgttaatcac cacatggtta ttctgctcaa acgtcccgga cgcctgcgac
     4561 cggctgtctg ccatgctgcc cggtgtaccg acataaccgc cggtggcata gccgcgcatc
     4621 agccggtaaa gattccccac gccaatccgg ctggttgcct ccttcgtgaa gacaaactca
     4681 ccacggtgaa caatccccgc tggctcatat ttgccgccgg ttcccgtaaa tcctccggtt
     4741 gcaaaatgga atttcgccgc agcggcctga atggctgtac cgcctgacgc ggatgcgccg
     4801 ccaccaacag ccccgccaat ggcgctgccg atactcccga caatccccac cattgcctgc
     4861 ttaagcagaa tttctgtcat catggacagc acggaacggg tgaagctgcg ccagttctgc
     4921 tcactgccgg tcagcatcgc cgccatattc tgtgcaatac catcaaaggt ctgcgtggct
     4981 gcacttttta cctgcgacat actgtccgtg gcgctctctt cccactcact ccagccggac
     5041 ttcaggcctg ccatccagtt cccgcgaagc tggtcttcag ccgcccaggt ctttttctgc
     5101 tctgacatga cgttattcag cgccagcgga ttatcgccat actgttcctt caggcgctgt
     5161 tccgtggctt cccgttctgc ctgccggtca gtcagccccc ggcttttcgc atcaatggcg
     5221 gcccgttttg cccgttgctg ctgtgcgaat ttatccgcct gctgcgccag cgcgttcagg
     5281 cgctcctgat acgtaacctt gtcgccaagt gcagccagct ggcgtttgta ctccagcgtc
     5341 tcatctttat gcgccagcag ggatttctcc tgtgcagaca gctggcgacg ttgcgccgcc
     5401 tcctccagta ccgcgaactg actctccgcc ttccacaaat cccggcgctg ctggctgatt
     5461 ttctcatttg ctccggcatg cttctccagc gtccggagtt ctgcctgaag cgtcagcagg
     5521 gcagcatgag cactgtcttc ctgacgatcg cccgcagaca ccttcacgct ggactgtttc
     5581 ggctttttca gcgtcgcttc ataatccttt ttcgccgccg ccatcagcgt gttgtaatcc
     5641 gcctgcagga ttttcccgtc tttcagtgcc ttgttcagtt cttcctgacg ggcggtatat
     5701 ttctccagcg gcgtctgcag ccgttcgtaa gccttctgcg cctcttcggt atatttcagc
     5761 cgtgacgctt cggtatcgct ctgctgctgc gcatttttgt cctgttgagt ctgctgctca
     5821 gccttctttc gggcggcttc aagcgcaaga cgggcctttt cacgatcatc ccagtaacgc
     5881 gcccgcgctt catcgttaac aaaataatca tccttgcgca gattccagat gtcgtctgct
     5941 ttcttatacg cagcctctgc cttaatcagc atctcctgcg cggtatcagg acgaccaata
     6001 tccagcaccg catcccacat ggatttgaat gcccgcgcag tcctgtctgc ccaggtctcc
     6061 agcgtgccca tgttctcttt caggcggcgg gtctggtcat caaacccttt cgttgcggcc
     6121 tcgttcgccg cctgcaatgc cccggcttca tcgccggaac gctgcaactg agcaacatac
     6181 gcaatctgct ccgccgacac gttatggaac tggcgagcca tcgccgtcag ccccgacgtc
     6241 gggtctgtgg tcagcttccc gaaggcttca gcgaccttgt ccacctccac gccggatgca
     6301 gaggagaaac gcgccacact ctggctgatg gacgcaatct gagcctcacc gcttaccccc
     6361 gccttaacca gtgcgctgag tgactcgctg gtctggttaa acgtcagccc tgccgcctgc
     6421 ccggctctgg acaggaccag catacgatct gccgtcagtc ccgcctgatt gccggaaagg
     6481 accagcgttt tgttgaaatc ggacagggtt gagttgccct gataccaggc atacgccagc
     6541 gcaccggtcg ccaccgccag cgaggtggcc cccaccatcg gcagggtgat cgcaccggca
     6601 agccccctga acatggggat catcccgccg aaggagtcct tcacctgccc cccctgttgc
     6661 agcaggatca gccacggact ttgcccgcct gcaagctgcg tggccacgtc ggtgaactgt
     6721 gcaggcagca tacgcatggc ggctttatac tgcccgacgg aaatccccgc tttctgtgca
     6781 gccagcgcct gtcggctcag cgactgttca acgactgccg ctgttttttt cgcatcactt
     6841 tccgtaccag aaaaatgacg cctgactctg gccatctgct cgtcaaatct ggccgcatcc
     6901 agactcaaat caacgaccag atcgcctacc ggttcagcca taccggactc ctcctgcgat
     6961 cccttctgat actgtcatca gcattacgtc atcctccgtc atgtccgcca catccgggga
     7021 agcggggata acttcattcc cgtccgggcc aaagcggaca cctccggcaa gccctgccgc
     7081 tttctgcatc agcacatcat cttcaggctc ttcgtcagcc tcgcgccggt tcagcagact
     7141 gaaatccagc ggatgcatat ccggatcgct gaaaaacagg ctgagcacgg tgtacgtcag
     7201 cccggaaaag tgcatatcca gcagaacatc atgaaaataa tgggtactgt aaaagcggtg
     7261 ccagtcggca tactccgtgg atgacatccc ggcaagcatg gcacgccagt cgggtcgccc
     7321 catctcacgc gccagtttca gggcaaaact cagctcaccg tcgaacactt tcccgcagaa
     7381 acaggctctg cgggcccggc gtcctctgtc tgttcagggg cattattcac cacaaactca
     7441 tacataccag acagccggta caccacgttt tcagcatgag aaattgcctc cgtgggccag
     7501 gtggtaagca cttcctgctc aatctgttta acggcttcat tcatggacgg catctgcgtc
     7561 ttctgcggat ggttatgcca cagggacatc gccaccagga tccacgcgcc ctgtagcggc
     7621 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     7681 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     7741 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     7801 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     7861 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     7921 ggtttaacac tcagaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7981 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     8041 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     8101 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     8161 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     8221 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     8281 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8341 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8401 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8461 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8521 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8581 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8641 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8701 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8761 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     8821 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8881 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8941 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     9001 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     9061 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     9121 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     9181 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     9241 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9301 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9361 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9421 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9481 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9541 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9601 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9661 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9721 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9781 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9841 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9901 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9961 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    10021 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    10081 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
    10141 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
    10201 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
    10261 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaa
//
