LOCUS       40924_38868        5013 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Phagemid vector pScaf-2268, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Nafisi PM, Aksel T, Douglas SM.
  TITLE     Construction of a novel phagemid to produce custom DNA origami 
            scaffolds
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Nafisi PM, Aksel T, Douglas SM.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2018) Cellular and Molecular Pharmacology, 
            University of California, San Francisco, 600 16th Street, Genentech 
            Hall S-474, San Francisco, CA 94158, USA
REFERENCE   3  (bases 1 to 5013)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5013)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-MAY-2018) Cellular and Molecular Pharmacology, University of 
            California, San Francisco, 600 16th Street, Genentech Hall S-474, 
            San Francisco, CA 94158, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      44..424
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     misc_feature    430..2305
                     /label=p2268 insert
                     /note="p2268 insert"
     rep_origin      2311..2631
                     /note="M13term; M13 origin truncated to eliminate initation
                     capabilities, resulting in M13 terminator"
     misc_feature    2592..2596
                     /label=term-alpha
                     /note="term-alpha"
     misc_feature    2610..2614
                     /label=term-beta
                     /note="term-beta"
     misc_feature    2619..2624
                     /label=term-gamma
                     /note="term-gamma"
     primer_bind     complement(2647..2663)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2671..2687)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2695..2725)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2740..2761)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3049..3637)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3811..4668)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4669..4773)
                     /label=AmpR promoter
ORIGIN
        1 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgacgcgcc ctgtagcggc
       61 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
      121 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
      181 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
      241 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
      301 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
      361 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
      421 tcggggtacc gcatgcctcg agttagacct gattcttgag aaacaaggcg gctccacact
      481 gaatccattg atcctgatta ccaccgcaag gcacatcgat gttggtcacg actctgtcgc
      541 gctcagcgga tgtgtacact ggcaaagaaa tgcactcatc gggtgagtat gggccgcaag
      601 cgtcttgagg aatccaaccc atgaagcatg gcagaacgct agagacgctt gggctgtcga
      661 gctgattctc ggacagttga ttaccgtcga aggagcagcc ttccaggagc agtcctgaga
      721 tcttgatctg gagtttggcc tcttgcagtc tacccttcca ggaggcgaca aatttcagag
      781 aatccactga tcttccaaca gcccttgctg tttcctggcg gagggcgttc aggaatgtgt
      841 cgggatggaa caattcagag aggtccagtg tttcggacaa gagggcctgc ttctctgctt
      901 tatcgaccca attctggatg gccagtgctc tagccacgag gcctctgaga tattgcagag
      961 ggtcttcggg tccctcccac ttggattgcc aggccagagg gcacttctgg ttgagcaggg
     1021 cagaggccaa cttctggact tctgatgaga gcagtgtagt gccgcggatc actttgctca
     1081 aggctgccag tgattgatga actgactgga ccaatctgat ggcgttgaac tgctccaaga
     1141 taataaagga cagaattggt gatccttggc ggtcattggg aggagggacc ttctggtgga
     1201 tcaggttgga gttctggttc agtttcttcc acaggttgag cactgggctg agttcattgc
     1261 tccagatctc gcgatcgaac tttgagcctg cagtaataga gcggcccagg atgcggagtt
     1321 gggaaatcac ctgagatgag atcatgcgct gtgatgagcg tgcaatgttg gcaggcagac
     1381 cgaagaagga tggcttatcg tcttctggaa tcttctcaat gacagccctg taatccagaa
     1441 tgctgcagct ttgtggcagg ctcacgctgt agggaaagat ggacttcttg ttgcgctgat
     1501 tgaacacgtc aatgacgcta gagttgaaga attgcttcaa atagctctgc aggacgcgca
     1561 agtcgaaata gttatcgatg cggcctccgt agatggcgtt ctccagcaga ccatggacaa
     1621 actcccattg cacgtccttg gcaccatcaa agagcctgtc aatgatattg taaccggccc
     1681 tcaggtcgga gagagagaac tcgtagaatt tggtccaacc ttgaggaatg tagttgcgcc
     1741 tctcttggca tgcggcgtga aaccaggcca gagagaacag agcatgtgcg cggtgagtgt
     1801 tatctttctt agagatttgt tcaggtgtcc aagattcata tgtgcgcatg agattcttct
     1861 tgagtccagg tggtgattca taagtgatct tgaggcttga ctggaggagg ataggtgtaa
     1921 agtttgggtg cacttcggct gtcaaccaga gcctgaaagt gtctttgggt tgcaaggtgt
     1981 tcagttcctt ctccaagaca ggcagccagg agacaaccag gtgcaagttc ttcaaacaca
     2041 gccagtcacc attccttgcg cattccttga gcatttggat agccaagtca gcttggccct
     2101 ggcccattgc aacctggtgg taacattctc ctgatctttc ggcgttggcg agctcctgga
     2161 gttcctggga gggatcggct cctggagaaa taatgatcag aatgggctcg atttcgagtg
     2221 tctccttgta cagcctcttg agattgagtg ggagtggaga gacttccttc aaacccaagg
     2281 tcttgcaggc aaagagggcc atagcggatc cacgcgccct gtagcggcgc attaagcgcg
     2341 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     2401 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     2461 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     2521 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     2581 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg agaattcgta
     2641 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     2701 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     2761 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     2821 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     2881 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     2941 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     3001 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     3061 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     3121 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     3181 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     3241 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     3301 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     3361 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     3421 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3481 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3541 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3601 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3661 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     3721 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     3781 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     3841 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     3901 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     3961 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     4021 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     4081 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     4141 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     4201 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     4261 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     4321 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     4381 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     4441 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     4501 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     4561 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     4621 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     4681 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     4741 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     4801 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     4861 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     4921 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     4981 agcgggtgtt ggcgggtgtc ggggctggct taa
//
