LOCUS       40924_38863        4257 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Phagemid vector pScaf-1512, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Nafisi PM, Aksel T, Douglas SM.
  TITLE     Construction of a novel phagemid to produce custom DNA origami 
            scaffolds
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Nafisi PM, Aksel T, Douglas SM.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2018) Cellular and Molecular Pharmacology, 
            University of California, San Francisco, 600 16th Street, Genentech 
            Hall S-474, San Francisco, CA 94158, USA
REFERENCE   3  (bases 1 to 4257)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4257)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-MAY-2018) Cellular and Molecular Pharmacology, University of 
            California, San Francisco, 600 16th Street, Genentech Hall S-474, 
            San Francisco, CA 94158, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4257
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      44..424
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     misc_feature    430..1549
                     /label=p1512 insert
                     /note="p1512 insert"
     rep_origin      1555..1875
                     /note="M13term; M13 origin truncated to eliminate initation
                     capabilities, resulting in M13 terminator"
     misc_feature    1836..1840
                     /label=term-alpha
                     /note="term-alpha"
     misc_feature    1854..1858
                     /label=term-beta
                     /note="term-beta"
     misc_feature    1863..1868
                     /label=term-gamma
                     /note="term-gamma"
     primer_bind     complement(1891..1907)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1915..1931)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1939..1969)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1984..2005)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2293..2881)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3055..3912)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3913..4017)
                     /label=AmpR promoter
ORIGIN
        1 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgacgcgcc ctgtagcggc
       61 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
      121 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
      181 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
      241 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
      301 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
      361 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
      421 tcggggtacc aactgctggg ccatatcgac atggacacca gagaatggag cgacggcgtg
      481 ctcacaaact ccgccagaca agtcgtgcgc gaacctcaag acgtcagctc ttggatcatc
      541 tgcgatggtg atattgaccc tgagtggatc gagtccctga attccgtgtt ggatgacaac
      601 aggctcctca caatgccttc tggtgagaga atccagttcg gtcctaacgt gaacttcgtg
      661 ttcgagacac acgatctcag ctgtgctagc ccagctacta tctcccgcat gggaatgatc
      721 ttcttgtccg acgaggagac agatttgaac tcattgatca agtcttggct cagaaaccag
      781 cctgcagaat ataggaataa cctggagaac tggatcggtg attacttcga gaaggctttg
      841 cagtgggtgc tgaaacagaa cgactatgtc gtcgaaacca gcctggtcgg tacagttatg
      901 aacggactct cccatctgca cggatgcaga gatcacgatg agtttatcat caatttgatc
      961 cgcggactgg gaggtaactt gaatatgaaa tctcgcctgg agttcactaa agaagtgttc
     1021 cactgggcta gggagtcacc acctgacttc cacaaaccta tggacaccta ctatgattcc
     1081 acaagaggca ggttggccac ctacgtgctg aagaagcctg aggacctcac cgctgacgac
     1141 ttctccaacg gactgactct gcccgtgatc cagactccag acatgcagcg cggactcgat
     1201 tactttaagc cctggctcag ctccgatacc aagcaacctt tcattctcgt gggaccagag
     1261 ggatgtggta aaggaatgct cctgaggtat gcattctccc agctccgctc aacccaaatt
     1321 gccactgttc actgttcagc ccaaacaact tcaaggcatc tcctccagaa gctcagccag
     1381 acctgtatgg ttatcagcac caacaccggc agagtttacc gcccaaagga ttgtgagcgc
     1441 ctcgtgttgt acctcaaaga tatcaatctg cccaaactcg ataagtgggg cacttccacc
     1501 ctcgtggcat ttctccaaca ggtgctgacc taccagggct tctacgacgg gatccacgcg
     1561 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     1621 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     1681 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     1741 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
     1801 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     1861 ttgttccaaa ctggagaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     1921 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     1981 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     2041 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     2101 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     2161 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2221 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2281 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2341 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2401 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2461 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2521 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2581 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2641 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2701 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     2761 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2821 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2881 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2941 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     3001 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     3061 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     3121 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     3181 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     3241 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     3301 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     3361 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     3421 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     3481 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     3541 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     3601 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     3661 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     3721 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     3781 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     3841 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     3901 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     3961 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     4021 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     4081 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
     4141 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     4201 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaa
//
