LOCUS       V003409                 7577 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003409
VERSION     V003409
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7577)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7577)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 7577)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7577)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(10..28)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(46..62)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(70..86)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(94..124)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(139..160)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(448..1036)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1210..2067)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2068..2172)
                     /label="AmpR promoter"
     rep_origin      complement(2506..2961)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3102..3118
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        3125..3143
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3692..4501
                     /gene="URA3"
                     /label="Orotidine 5'-phosphate decarboxylase"
                     /note="Orotidine 5'-phosphate decarboxylase from Candida
                     albicans (strain SC5314 / ATCC MYA-2876). Accession#:
                     P13649"
     promoter        5030..5380
                     /label="MET17 promoter"
                     /note="expression is repressed by methionine"
     CDS             5381..6712
                     /label="MET17"
                     /note="O-acetylserine and O-acetylhomoserine sulfhydrylase,
                     required for sulfur amino acid synthesis"
     misc_feature    6716..7298
                     /label="3' UTR of ScMET15"
                     /note="3' UTR of ScMET15"
     terminator      7327..7574
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
ORIGIN
        1 cttgagtatt ctatagtgtc acctaaatag cttggcgtaa tcatggtcat agctgtttcc
       61 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
      121 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
      181 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
      241 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
      301 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      361 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
      421 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
      481 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      541 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      601 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
      661 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
      721 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
      781 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
      841 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
      901 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
      961 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1021 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1081 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     1141 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     1201 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     1261 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     1321 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     1381 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     1441 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     1501 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     1561 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     1621 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     1681 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     1741 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     1801 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     1861 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     1921 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     1981 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     2041 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     2101 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     2161 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     2221 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
     2281 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     2341 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     2401 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     2461 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgaaatt gtaaacgtta
     2521 atattttgtt aaaattcgcg ttaaatattt gttaaatcag ctcatttttt aaccaatagg
     2581 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     2641 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     2701 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc acccaaatca agttttttgc
     2761 ggtcgaggtg ccgtaaagct ctaaatcgga accctaaagg gagcccccga tttagagctt
     2821 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     2881 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     2941 atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggcg
     3001 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     3061 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     3121 attgtaatac gactcactat agggcgaatt ggttttccaa tgatgagcac ttttaaagtt
     3181 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     3241 atacactatt ctcagaatga cttggttgag tactaatagg aattgatttg gatggtataa
     3301 acggaaacaa aaaaaagagc tggtactact ttctttaaaa ttattttatt atttgatttt
     3361 atttaatagt atatattata ttttgaacgt agattatttt gttgaaagtt gctgtagtgc
     3421 cattgattcg taacactaat tctgtattag tcattcctct tgtttgatag tatccaaaaa
     3481 aacggctatt tttttgcaat cttatttcct gcatattata cagataacat aatgaaagaa
     3541 aaaatctttt tttttgttct tcaatgatga tttcaaccat tcttttaaac attgatcaat
     3601 tcctgagcaa caaccccata cacactggtt tatataccgc cccttttaca gttgaagaaa
     3661 gaaatagaaa tagaaatagc aaacaaaaga tatgacagtc aacactaaga cctatagtga
     3721 gagagcagaa actcatgcct caccagtagc acagcgatta tttcgattaa tggaactgaa
     3781 gaaaaccaat ttatgtgcat caattgacgt tgataccact aaggaattcc ttgaattaat
     3841 tgataaatta ggtccttatg tatgcttaat caagactcat attgatataa tcaatgattt
     3901 ttcctatgaa tccactattg aaccattatt agaactttca cgtaaacatc aatttatgat
     3961 ttttgaagat agaaaatttg ctgatattgg taataccgta aagaaacaat atattggtgg
     4021 agtttataaa attagtagtt gggcagatat taccaatgct catggtgtca ctgggaatgg
     4081 agtggttgaa ggattaaaac agggagctaa agaaaccacc accaaccaag agccaagagg
     4141 gttattgatg ttagctgaat tatcatcagt gggatcatta gcatatggag aatattctca
     4201 aaaaactgtt gaaattgcta aatccgataa ggaatttgtt attggattta ttgcccaacg
     4261 tgatatgggt ggccaagaag aaggatttga ttggcttatt atgacacctg gagttggatt
     4321 agatgataaa ggtgatggat taggacaaca atatagaact gttgatgaag ttgttagcac
     4381 tggaactgat attatcattg ttggtagagg attgtttggt aaaggaagag atccagatat
     4441 tgaaggtaaa aggtatagaa atgctggttg gaatgcttat ttgaaaaaga ctggccaatt
     4501 ataaatgtga agggggagat tttcacttta ttagatttgt atatatgtag aataaataaa
     4561 taaataagtt aaataaataa ttaaataagg gtggtaatta ttactattta caatcaaagg
     4621 tggtccttct agctgtaatc cgggcagcgc aacggaacat tcatcagtgt aaaaatggaa
     4681 tcaataaagc cctgcgcagc gcgcagggtc agcctgaata cgcgtttaat gaccagcaca
     4741 gtcgtgatgg caaggtcaga atagcccaag tcggccgagg ggcctgtaca gtgagggaag
     4801 atcctagtta gtagatgata gttgattttt attccaacac taagaaataa tttcgccatt
     4861 tcttgaatgt atttaaagat atttaatgct ataatagaca tttaaatcca attcttccaa
     4921 catacaatgg gagtttggcc gagtggttta aggcgtcaga tttaggtgga tttaacctct
     4981 aaaatctctg atatcttcgg atgcaagggt tcgaatccct tagctctcat tattttttgc
     5041 tttttctctt gaggtcacat gatcgcaaaa tggcaaatgg cacgtgaagc tgtcgatatt
     5101 ggggaactgt ggtggttggc aaatgactaa ttaagttagt caaggcgcca tcctcatgaa
     5161 aactgtgtaa cataataacc gaagtgtcga aaaggtggca ccttgtccaa ttgaacacgc
     5221 tcgatgaaaa aaataagata tatataaggt taagtaaagc gtctgttaga aaggaagttt
     5281 ttcctttttc ttgctctctt gtcttttcat ctactatttc cttcgtgtaa tacagggtcg
     5341 tcagatacat agatacaatt ctattacccc catccataca atgccatctc atttcgatac
     5401 tgttcaacta cacgccggcc aagagaaccc tggtgacaat gctcacagat ccagagctgt
     5461 accaatttac gccaccactt cttatgtttt cgaaaactct aagcatggtt cgcaattgtt
     5521 tggtctagaa gttccaggtt acgtctattc ccgtttccaa aacccaacca gtaatgtttt
     5581 ggaagaaaga attgctgctt tagaaggtgg tgctgctgct ttggctgttt cctccggtca
     5641 agccgctcaa acccttgcca tccaaggttt ggcacacact ggtgacaaca tcgtttccac
     5701 ttcttactta tacggtggta cttataacca gttcaaaatc tcgttcaaaa gatttggtat
     5761 cgaggctaga tttgttgaag gtgacaatcc agaagaattc gaaaaggtct ttgatgaaag
     5821 aaccaaggct gtttatttgg aaaccattgg taatccaaag tacaatgttc cggattttga
     5881 aaaaattgtt gcaattgctc acaaacacgg tattccagtt gtcgttgaca acacatttgg
     5941 tgccggtggt tacttctgtc agccaattaa atacggtgct gatattgtaa cacattctgc
     6001 taccaaatgg attggtggtc atggtactac tatcggtggt attattgttg actctggtaa
     6061 gttcccatgg aaggactacc cagaaaagtt ccctcaattc tctcaacctg ccgaaggata
     6121 tcacggtact atctacaatg aagcctacgg taacttggca tacatcgttc atgttagaac
     6181 tgaactatta agagatttgg gtccattgat gaacccattt gcctctttct tgctactaca
     6241 aggtgttgaa acattatctt tgagagctga aagacacggt gaaaatgcat tgaagttagc
     6301 caaatggtta gaacaatccc catacgtatc ttgggtttca taccctggtt tagcatctca
     6361 ttctcatcat gaaaatgcta agaagtatct atctaacggt ttcggtggtg tcttatcttt
     6421 cggtgtaaaa gacttaccaa atgccgacaa ggaaactgac ccattcaaac tttctggtgc
     6481 tcaagttgtt gacaatttaa agcttgcctc taacttggcc aatgttggtg atgccaagac
     6541 cttagtcatt gctccatact tcactaccca caaacaatta aatgacaaag aaaagttggc
     6601 atctggtgtt accaaggact taattcgtgt ctctgttggt atcgaattta ttgatgacat
     6661 tattgcagac ttccagcaat cttttgaaac tgttttcgct ggccaaaaac catgagtgtg
     6721 cgtaatgagt tgtaaaatta tgtataaacc tactttctct cacaagtact atacttttat
     6781 aaaacgaact ttattgaaat gaatatcctt tttttccctt gttacatgtc gtgactcgta
     6841 ctttgaacct aaattgttct aacatcaaag aacagtgtta attcgcagtc gagaagaaaa
     6901 atatggtgaa caagactcat ctacttcatg agactacttt acgcctccta taaagctgtc
     6961 acactggata aatttattgt aggaccaagt tacaaaagag gatgatggag gtttctttac
     7021 aataaagaag cacatgtgtg ttaacgtttt tagtatttgc ttgttatgta aatcaggaaa
     7081 acttcgcggg atttggttgg atgctacttt ccatacaata aatattatag atctaaaaag
     7141 ccaaattaca agtaaagatt agtaaagctg ttggaattcc atcgttgata aaaatgttag
     7201 ttattaaata taaaagtcag aataggtgaa cttggattta attgttggca tttcgttgct
     7261 gctagaggcc ataatattag atagccagga catactagga tccgtccccc ttttcctttg
     7321 tcgatatcat gtaattagtt atgtcacgct tacattcacg ccctcccccc acatccgctc
     7381 taaccgaaaa ggaaggagtt agacaacctg aagtctaggt ccctatttat ttttttatag
     7441 ttatgttagt attaagaacg ttatttatat ttcaaatttt tctttttttt ctgtacagac
     7501 gcgtgtacgc atgtaacatt atactgaaaa ccttgcttga gaaggttttg ggacgctcga
     7561 aggctttaat ttgcaag
//
