LOCUS       V003514                 5873 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003514
VERSION     V003514
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5873)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5873)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5873)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5873
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..38
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     misc_feature    651..1735
                     /label="5' flanking region of A. nidulans argB"
                     /note="5' flanking region of A. nidulans argB"
     misc_feature    1736..1788
                     /label="5' UTR of A. nidulans argB"
                     /note="5' UTR of A. nidulans argB"
     CDS             1789..2865
                     /gene="argB"
                     /label="Ornithine carbamoyltransferase, mitochondrial"
                     /note="Ornithine carbamoyltransferase, mitochondrial from
                     Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS
                     112.46 / NRRL 194 / M139). Accession#: P11803"
     regulatory      2869..3255
                     /label="3' UTR of A. nidulans argB, incl. polyA"
                     /note="3' UTR of A. nidulans argB, incl. polyA"
                     /regulatory_class="terminator"
     rep_origin      complement(4046..4634)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4808..5665)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(5666..5770)
                     /label="AmpR promoter"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
      181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
      241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
      301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
      361 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
      421 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
      481 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
      541 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
      601 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgacggcg
      661 acggagcgca agccctcaat gtcgccagca atcaaatcgg cttcgaagtc aaggtgattc
      721 acatggccgt tgcgaatgct aaccttgatg gaaggcaagg atgcagatcc aacaagggca
      781 ttttcgtatt ccggtaagcg tacaattact tcagactggc cggtttcgac ctctttccca
      841 atccaggtga cgaaacgccc aatatttctc gccgattcac ttttgactcg atcagcatcg
      901 aggacaatat ccccttgtac ccttgccctg acaccttcgc ttgtggccga cacgactgat
      961 aggtttgtcg gtctaaatat ggctgcttcc ttgacgtatt gctttactac ctcgggaaca
     1021 aagaaggcca gaatgaggat gacaatgacg gaaccgacag caaggaggcc gcataatacc
     1081 gtccaccaag ctttatttcg cggttttttg gggtagtcat ctaatgaaac agacccggac
     1141 gcagcagagg aagcccgcga tgactctata ccaccgtacg ccgatatatc atcatcgcgg
     1201 cgatggagaa gtggggttga ctccgaagac acttcaaagg agcgacgctg ttgatttgta
     1261 gacgacgctt gatagggaga agcattattg tcgtgatgct cgcccaacag aggccgactc
     1321 gcctcatccg tcataacgaa cgctgtgtaa agcggagtgg gggggaaagt gtggattgtg
     1381 gagagtatgc gatagtgttg aggctgatca gacggcgaat cgggccagat atgaccagtt
     1441 tagaggcctc atttgactat aatttacata aattagataa atagagatga acgcatgcaa
     1501 taattgcagc aaatattgat gaagcgagag gtaggacgat gaaggactgt gagcagttca
     1561 aggtatcagc agagtcaagg gcctgatgca atggcggtga tccgtgatca gcgaacggaa
     1621 ggggcgctaa ctctgtttct ttaccaatga tcggaagctc ctgctggcgg acttatgagt
     1681 cattcacgaa tcatttctca gttatttgtg gatgccctcg ttctgtccac aatttctttc
     1741 cgccccaagt cttttaagtt ctttaacatc tatattcttg cacttccaat ggcatccctt
     1801 cgctccgtac tcaagagcca gagcttgcga cacaccgtgc gatcctactc ctcgcaaacc
     1861 atgcctcccg cctcaccctt tgctccccgc cacttcctct ccattgcgga cctctcgccc
     1921 tccgagttcg caacccttgt tcgcaatgcc tcctcacaca aacgggctat caagtcgggg
     1981 tcaatgcccc aaaacttgca gggatcactc cttgggaaaa ctgtggccat gatcttcagc
     2041 aaacgaagca cgaggacaag ggtatctaca gaaggggccg ttgtgcagat gggaggtcat
     2101 ccgatgttct tgggcaagga tgatatccaa ctaggtgtca acgagtccct atacgacacc
     2161 tccgttgtca tttcgtccat ggtatcctgc attgtagccc gtgtcggtaa acatgcagag
     2221 gtcgcagatc tggcgaagca ctcttcggtt ccagtcatca atgctttgtg tgactctttc
     2281 caccctctcc aagccgtggc cgatttccag accatctatg aagcattcac ccccaaggcg
     2341 caccaccttt caagtctagg gttggaagga ttgaagatcg cttgggtggg tgacgccaac
     2401 aacgtcctgt tcgatatggc cattgctgct acaaaaatgg gtgtcgacat tgctgtcgct
     2461 actcccaagg ggtacgaaat ccctcctcac atgctggagc tcatcaagtc tgctggagag
     2521 ggtgtctcga aaccaggaaa gcttctgcaa accaatattc ccgaagaagc ggtcaaggac
     2581 gccgatattc tggtcacaga cacctgggtc tctatgggcc aagaggaaga gaaggctcag
     2641 aggctgaagg agtttgatgg tttccaaatc actgctgaac tcgccaagcg aggaggagct
     2701 aaggagggct ggaagttcat gcactgtctc ccgcgacacc ctgaggaggt cagcgacgag
     2761 gttttctaca gcaaccggtc acttgtcttc cctgaggctg agaaccggtt atgggctgcg
     2821 atttccgcct tggagggttt cgttgtcaat aagggaaaaa ttgaataaat ataaccaggc
     2881 ttccatttaa atatatagag gctggcgtta tcaaactgat gagttgacgg gtatgagatc
     2941 attccgtccc taaatatatt tactccgatc acgtaaaagc ctgttagtag aagcattttc
     3001 ccaattatcc tgaccaattc tttctagcat atatcaaata actaattgac atgttctctc
     3061 gcttccttat attactcaga gtattggaaa tggggcaaat cgcacccggt gactttcaca
     3121 tgtcacgaat gcggagtcgt cctagccaag gtagatccag gcctaacaca ccccaaccct
     3181 cgactctcct cattccatac tcttgacctc tatccagcac attcttctga ggtttcgcaa
     3241 tggctgtagg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg
     3301 cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag
     3361 gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga
     3421 cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg
     3481 tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg
     3541 ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca
     3601 ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca
     3661 ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc
     3721 taacttcgat cactggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat
     3781 ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc
     3841 gtcgcggtgc atggagccgg gccacctcga cctgaatgga agccggcggc acctcgctaa
     3901 cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg tgaatgcgca
     3961 aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc gcacgcggcg
     4021 catctcgggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4081 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     4141 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     4201 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     4261 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     4321 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     4381 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     4441 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     4501 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     4561 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     4621 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     4681 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     4741 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     4801 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     4861 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     4921 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     4981 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     5041 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     5101 gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     5161 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     5221 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     5281 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     5341 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     5401 gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag
     5461 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     5521 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     5581 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     5641 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     5701 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     5761 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     5821 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaa
//
