LOCUS       40924_38433        5720 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Integration vector pSac-Kan, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Middleton R, Hofmeister A.
  TITLE     New shuttle vectors for ectopic insertion of genes into Bacillus 
            subtilis
  JOURNAL   Plasmid 51 (3), 238-245 (2004)
  PUBMED    15109830
REFERENCE   2  (bases 1 to 5720)
  AUTHORS   Middleton RH, Hofmeister AEM.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2003) Plant and Microbial Biology, University of 
            California, Berkeley, 281 Koshland Hall, Berkeley, CA 94704, USA
REFERENCE   3  (bases 1 to 5720)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5720)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2004"; volume: "51"; issue: "3"; pages: "238-245"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-NOV-2003) Plant and Microbial Biology, University of California,
            Berkeley, 281 Koshland Hall, Berkeley, CA 94704, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5720
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(463..1254)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    complement(2229..2873)
                     /note="sucrase-6-phosphate hydrolase (sacA) from Bacillus 
                     subtilis"
     promoter        3278..3382
                     /label=AmpR promoter
     CDS             3383..4240
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4414..5002
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(5241..5717)
                     /note="sucrase-6-phosphate hydrolase (sacA) from Bacillus 
                     subtilis"
ORIGIN
        1 cgcgcctaaa cggatatcat catcgctcat ccatgtcgac gctctccctt atgcgactcc
       61 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat
      121 ggtgcatgcc gacgtcctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg
      181 ttgaggccga agagcaccgc cgccgcaagg aatggtgcat gcctgcaggt cctggcgtaa
      241 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
      301 gcgatacaaa ttcctcgtag gcgctcggga cccctatcta gcgaactttt agaaaagata
      361 taaaacatca gagtatggac agttgcggat gtacttcaga aaagattaga tgtctaaaaa
      421 gcttgtagtt aaagcttttt agacatctaa atctaggtac taaaacaatt catccagtaa
      481 aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa aaaatagctc
      541 gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg caatgtcata
      601 ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt tgccatcttt
      661 cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt
      721 ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt cttcttccca
      781 gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt cggctaagcg
      841 gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa agagcctgat
      901 gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat actcttccga
      961 gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat gccgttcaaa
     1021 gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt ccttttcccg
     1081 ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat ataggttttc
     1141 attttctccc accagcttat ataccttagc aggagacatt ccttccgtat cttttacgca
     1201 gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag ccatttatta
     1261 tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg
     1321 aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca
     1381 aagttgtttt gaaagttggc gtataacata gtatcgacgg agccgatttt gaaaccacaa
     1441 ttatgataga atttacaagc tataaggtta ttgtcctggg tttcaagcat tagtccatgc
     1501 aagtttttat gctttgccca ttctatagat atattgataa gcgcgctgcc tatgccttgc
     1561 cccctgaaat ccttacatac ggcgatatct tctatataaa agatatatta tcttatcagt
     1621 attgtcaata tattcaaggc aatctgcctc ctcatcctct tcatcctctt cgtcttggta
     1681 gctttttaaa tatggcgctt catagagtaa ttctgtaaag gtccaattct cgttttcata
     1741 cctcggtata atcttaccta tcacctcaaa tggttcgctg ggtttatcgc ctgatgcggt
     1801 attttctcct tacgcatctg tgcggtattt cacaccgcat atgatcagat cttaaggcct
     1861 aggtctagag tctttgtttt gacgccatta gcgtacgtaa caatcctcgt taaaggacaa
     1921 ggacctgagc ggaagtgtat cgtacagtag acggagtata ctagtatagt ctatagtccg
     1981 tggaattatt atatttatct ccgacgatat tctcatcagt gaaatccagg ggtaccgagc
     2041 tcgaattcga taagcttcta ggatcctgag cgccggtcgc taccattacc agttggtctg
     2101 gtgtcaaaaa taataataac cgggcaggcc atgtctgccc gtatttcgcg taaggaaatc
     2161 cattatgtac tatttcgatc agaccagttt ttaatttgtg tgtttccatg tgtccagttt
     2221 ggcgcgccaa aatcgtccag cccgttgaag cccgcgccgg ttatcgggcc aagaaatctc
     2281 cactctgtca ggttatcaga agcaaacaac acagcctgcc ctttcaaatt ctctgtttgc
     2341 gcaccaatca ccatatacca tgtgccttca tgctcccata cttcggatcg cgaaaagtgc
     2401 gccgttaata tcgcttccgg aaggcgggcg acgacaccct ttttctcaaa ggacagcccg
     2461 tcgtcagaaa cagcaaggca ttgatacgtt tcccgatttc catcctgatc cctgacattt
     2521 cctgtgtaaa aaagatagag ccgatcgtct ttcgtgacag cgctgcccga gtagcagccg
     2581 tttttatcaa accaatcact cggagccagc gcaatctctt cccgcttcca attcacaaca
     2641 tcctgtgtcg tataatgccc ccaaaatttt gcgccgtgcc ccgtctgaaa cggctgccac
     2701 tgaaagaata catgatagct gcccttccaa taaatcacgc cattcgggtc attcagcagc
     2761 ccaaccggcg gcatgatatg aaaatgctgg cggtgcggat cgctgttagc gatgggctct
     2821 tttttctcca cttcttcata agcccggcga cgaagctcct ggtcatgtgc tgtcctggcg
     2881 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     2941 atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
     3001 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     3061 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     3121 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     3181 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     3241 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     3301 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     3361 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     3421 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     3481 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     3541 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     3601 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     3661 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     3721 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     3781 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     3841 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     3901 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     3961 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     4021 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     4081 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     4141 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     4201 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     4261 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     4321 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     4381 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     4441 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     4501 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     4561 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     4621 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     4681 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     4741 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     4801 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     4861 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     4921 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     4981 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     5041 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     5101 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     5161 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     5221 gccgattcat taatgcagga tttcccggtc gcactggctg ttacttcatg atttcccggg
     5281 aaaggaaaat atcttgcact aaagacctct tctccgttat tgataaagat ttccacagat
     5341 gacgcgtcga gaaacatgtg tacggtatgc aaatccttga tgcggcaatg tctcgcttct
     5401 gttctttttc cgtcaaagct ctttcgttcc agcgtaacaa tcccctcgtc tttatggaag
     5461 gaaaacgtag ccgttccgcg gatactgatt gaaaagccag actccgtatg aatgtcttcc
     5521 agtagaatct cagttctttc aggtttttcc actggaagcg caccagatga tccatgcatg
     5581 ttgatgtgta tttttttctc atttctgcgc atggctttta gctcaggcag cggctgctga
     5641 atcagcttct gtcctgaaag cgtcagctgt ctcggcagcg tcatgcagtg aatccagtgg
     5701 cagtcaatgg tcggatgggg
//
