LOCUS       V003516                 5045 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003516
VERSION     V003516
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Middleton R, Hofmeister A.
  TITLE     New shuttle vectors for ectopic insertion of genes into Bacillus
            subtilis
  JOURNAL   Plasmid 51 (3), 238-245 (2004)
   PUBMED   15109830
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   Middleton RH, Hofmeister AEM.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-NOV-2003) Plant and Microbial Biology, University of
            California, Berkeley, 281 Koshland Hall, Berkeley, CA 94704, USA
REFERENCE   3  (bases 1 to 5045)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5045)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid";
            date: "2004"; volume: "51"; issue: "3"; pages: "238-245"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (13-NOV-2003) Plant and Microbial Biology, University of California,
            Berkeley, 281 Koshland Hall, Berkeley, CA 94704, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5045
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    56..700
                     /note="sucrase-6-phosphate hydrolase (sacA) from Bacillus
                     subtilis"
     CDS             complement(1115..1762)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     misc_feature    2257..2733
                     /note="sucrase-6-phosphate hydrolase (sacA) from Bacillus
                     subtilis"
     promoter        3139..3243
                     /label="AmpR promoter"
     CDS             3244..4101
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4275..4863
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcaggacagc
       61 acatgaccag gagcttcgtc gccgggctta tgaagaagtg gagaaaaaag agcccatcgc
      121 taacagcgat ccgcaccgcc agcattttca tatcatgccg ccggttgggc tgctgaatga
      181 cccgaatggc gtgatttatt ggaagggcag ctatcatgta ttctttcagt ggcagccgtt
      241 tcagacgggg cacggcgcaa aattttgggg gcattatacg acacaggatg ttgtgaattg
      301 gaagcgggaa gagattgcgc tggctccgag tgattggttt gataaaaacg gctgctactc
      361 gggcagcgct gtcacgaaag acgatcggct ctatcttttt tacacaggaa atgtcaggga
      421 tcaggatgga aatcgggaaa cgtatcaatg ccttgctgtt tctgacgacg ggctgtcctt
      481 tgagaaaaag ggtgtcgtcg cccgccttcc ggaagcgata ttaacggcgc acttttcgcg
      541 atccgaagta tgggagcatg aaggcacatg gtatatggtg attggtgcgc aaacagagaa
      601 tttgaaaggg caggctgtgt tgtttgcttc tgataacctg acagagtgga gatttcttgg
      661 cccgataacc ggcgcgggct tcaacgggct ggacgatttt ggcgcgccta aacggatatc
      721 atcatcgctc atccatgtcg acgctctccc ttatgcgact cctgcattag gaagcagccc
      781 agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg
      841 gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc
      901 atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca
      961 gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatctgg
     1021 agctgtaata taaaaacctt cttcaactaa cggggcaggt tagtgacatt agaaaaccga
     1081 ctgtaaaaag tacagtcggc attatctcat attataaaag ccagtcatta ggcctatctg
     1141 acaattcctg aatagagttc ataaacaatc ctgcatgata accatcacaa acagaatgat
     1201 gtacctgtaa agatagcggt aaatatattg aattaccttt attaatgaat tttcctgctg
     1261 taataatggg tagaaggtaa ttactattat tattgatatt taagttaaac ccagtaaatg
     1321 aagtccatgg aataatagaa agagaaaaag cattttcagg tataggtgtt ttgggaaaca
     1381 atttccccga accattatat ttctctacat cagaaaggta taaatcataa aactctttga
     1441 agtcattctt tacaggagtc caaataccag agaatgtttt agatacacca tcaaaaattg
     1501 tataaagtgg ctctaactta tcccaataac ctaactctcc gtcgctattg taaccagttc
     1561 taaaagctgt atttgagttt atcacccttg tcactaagaa aataaatgca gggtaaaatt
     1621 tatatccttc ttgttttatg tttcggtata aaacactaat atcaatttct gtggttatac
     1681 taaaagtcgt ttgttggttc aaataatgat taaatatctc ttttctcttc caattgtcta
     1741 aatcaatttt attaaagttc atttgatatg cctcctaaat ttttatctaa agtgaattta
     1801 ggaggcttac ttgtctgctt tcttcattag aatcaatcct tttttaaaag tcaatattac
     1861 tgtaacataa atatatattt taaaaatatc ccactttatc caattttcgt ttgttgaact
     1921 aatgggtgct ttagttgaag aataaagacc acattaaaaa atgtggtctt ttgtgttttt
     1981 ttaaaggatt tgagcgtagc gaaaaatcct tttctttctt atcttgataa taagggtaac
     2041 tattgccgat gataagctgt caaacatgag aattcgataa gcttctagga tcctgagcgc
     2101 cggtcgctac cattaccagt tggtctggtg tcaaaaataa taataaccgg gcaggccatg
     2161 tctgcccgta tttcgcgtaa ggaaatccat tatgtactat ttcgatcaga ccagttttta
     2221 atttgtgtgt ttccatgtgt ccagtttggc gcgccccatc cgaccattga ctgccactgg
     2281 attcactgca tgacgctgcc gagacagctg acgctttcag gacagaagct gattcagcag
     2341 ccgctgcctg agctaaaagc catgcgcaga aatgagaaaa aaatacacat caacatgcat
     2401 ggatcatctg gtgcgcttcc agtggaaaaa cctgaaagaa ctgagattct actggaagac
     2461 attcatacgg agtctggctt ttcaatcagt atccgcggaa cggctacgtt ttccttccat
     2521 aaagacgagg ggattgttac gctggaacga aagagctttg acggaaaaag aacagaagcg
     2581 agacattgcc gcatcaagga tttgcatacc gtacacatgt ttctcgacgc gtcatctgtg
     2641 gaaatcttta tcaataacgg agaagaggtc tttagtgcaa gatattttcc tttcccggga
     2701 aatcatgaag taacagccag tgcgaccggg aaatcctggc gtaatagcga agaggcccgc
     2761 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
     2821 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
     2881 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     2941 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     3001 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     3061 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     3121 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     3181 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     3241 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     3301 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     3361 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     3421 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     3481 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     3541 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     3601 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     3661 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     3721 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     3781 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     3841 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     3901 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     3961 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     4021 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     4081 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     4141 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     4201 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     4261 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     4321 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     4381 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     4441 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     4501 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     4561 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     4621 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     4681 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     4741 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     4801 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     4861 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     4921 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     4981 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     5041 gaaga
//
