LOCUS       62056_19175        3160 bp DNA     circular SYN 14-OCT-2021
DEFINITION  Binary vector pSaBIN2 DNA, complete sequence.
ACCESSION   LC652997
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Banno H.
  TITLE     Plant transient expression vector pSaBIN
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Banno H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2021) Contact:Hiroharu Banno Chubu University, 
            College of Bioscience & Biotechnology; 1200 Matsumoto-cho, Kasugai, 
            Aichi 4878501, Japan
REFERENCE   3  (bases 1 to 3160)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-OCT-2021) Contact:Hiroharu Banno Chubu University, College of 
            Bioscience & Biotechnology; 1200 Matsumoto-cho, Kasugai, Aichi 
            4878501, Japan"
FEATURES             Location/Qualifiers
     source          1..3160
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             250..906
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     rep_origin      1072..1660
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(2161..2185)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    2196..2246
                     /label=multiple cloning sites
                     /note="multiple cloning sites"
     misc_feature    complement(2298..2322)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     rep_origin      complement(2681..3116)
                     /direction=LEFT
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 agtgggttac atcgaactgg atcgtgggtt acatcgaact ggatctcaac agcggtaaga
       61 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      121 tatgtggcgc ggtattatcc cgtattgacg agcttggtaa gaggttccaa ctttcaccat
      181 aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag
      241 gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat
      301 cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt
      361 cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg
      421 gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaatttcg tatggcaatg
      481 aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag
      541 caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg gcagtttcta
      601 cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg
      661 tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac cagttttgat
      721 ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg caaatattat
      781 acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc cgtttgtgat
      841 ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga gtggcagggc
      901 ggggcgtaat ttttttaagg cagttattgg tgcctagaaa tattttatct gattaataag
      961 atgatcttct tgagatcgtt ttggtctgcg caagctcatg accaaaatcc cttaacgtga
     1021 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     1081 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     1141 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     1201 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     1261 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     1321 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     1381 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     1441 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     1501 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     1561 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     1621 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     1681 tttacggttc ctggcctttt gctggccttt tgctcacatg agatcacaga cccggattga
     1741 aggcgagatc cagcaactcg cgccagatca tcctgtgacg gaactttggc gcgtgatgac
     1801 tggccaggac gtcggccgaa agagcgacaa gcagatcacg cttttcgaca gcgtcggatt
     1861 tgcgatcgag gatttttcgg cgctgcgcta cgtccgcgac cgcgttgagg gatcaagcca
     1921 cagcagccca ctcgaccttc tagccgaccc agacgagcca agggatcttt ttggaatgct
     1981 gctccgtcgt caggctttcc gacgtttggg tggttgaaca gaagtcatta tcgcacggaa
     2041 tgccaagcac tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga
     2101 taaacctttt cacgcccttt taaatatccg attattctaa taaacgctct tttctcttag
     2161 gtttacccgc caatatatcc tgtcaaacac tgatagttta aacggatctc ctgcaggacc
     2221 accggtgact agtgatcctc ctcgaggtac attaaaaacg tccgcaatgt gttattaagt
     2281 tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc
     2341 cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac
     2401 ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc
     2461 ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat
     2521 gttgattgta acgatgacag agcgttgctg cctgtgatct aattccgggg atcggaaatc
     2581 cagaagcccg agaggttgcc gcctttcggg ctttttcttt ttcaaaaaaa aaaatttata
     2641 aaacgatctg ttgcggccgg ccgccgggtt gtgggcaaag gcgctcgacg gtgggcaacc
     2701 gcttgcggtt gtccacgggc ggagccggtg cgcgtagcgc attgtccaca agccaagggc
     2761 gaccaataat tgatatatat attcataatt gaaaagctaa ttgaacatac tacttgctgt
     2821 aactacttgc cggagcgagg ggtgtttgca agctgttgat ctgaaagggc tattagcgtt
     2881 ctcacgtgcc tttttgatta gcgatttcac gtgaccttat tagcgatttc acgtactccg
     2941 attagcgatt tcacgtaccc tgattagcga tttcacgtgg atagtttttg gagcgggccg
     3001 gaaagccccg tgaatcaagg ctttgcgggg cattagcggt ttcacgtgga taactaccct
     3061 ctatccacag gcttccgggg ataaaaaagc ccgctcgacg gcgggctgtt ggatggggat
     3121 cgcctgaatc gccccatcat ccagccagaa agtgacctag
//
