LOCUS       40924_41969        6268 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSX, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6268)
  AUTHORS   Owen JG, Ackerley DF.
  TITLE     Characterization of pyoverdine and achromobactin in Pseudomonas 
            syringae pv. phaseolicola 1448a
  JOURNAL   BMC Microbiol. 11 (1), 218 (2011)
  PUBMED    21967163
REFERENCE   2  (bases 1 to 6268)
  AUTHORS   Ackerley DF, Owen JG.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-SEP-2011) School of Biological Sciences, Victoria 
            University, Kelburn Parade, Wellington, NA 6140, New Zealand
REFERENCE   3  (bases 1 to 6268)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6268)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Microbiol."; date: "2011"; volume: "11"; issue: "1"; pages: "218"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-SEP-2011) School of Biological Sciences, Victoria University, 
            Kelburn Parade, Wellington, NA 6140, New Zealand"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6268
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2014..3093)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(3094..3171)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        3401..3429
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    3433..3457
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3472..3494
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     misc_feature    3498..3527
                     /note="multiple cloning sited region; contains recognition
                     sites for NdeI, BamHI, XbaI, SalI and HindIII"
     terminator      3730..3816
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3908..3935
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     CDS             complement(4011..4841)
                     /label=pRO1600 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pRO1600 from Pseudomonas aeruginosa"
     rep_origin      4855..5206
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     promoter        5256..5284
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             5473..6003
                     /label=GmR
                     /note="gentamycin acetyltransferase"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcaccggt tcactgcccg ctttccagtc gggaaacctg
     2041 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     2101 cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc
     2161 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc
     2221 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc
     2281 cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc
     2341 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat
     2401 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg
     2461 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac
     2521 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc
     2581 cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc
     2641 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc
     2701 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg
     2761 caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc
     2821 acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc
     2881 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac
     2941 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc
     3001 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata
     3061 ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc
     3121 cgggcgctat catgccatac cgcgaaaggt tttgcaccat tcgatggtgt caacgtaaat
     3181 gccgcttcgc cttcgcgcgc gaattgcaag ctgatccggg cttatcgact gcacggtgca
     3241 ccaatgcttc tggcgtcagg cagccatcgg aagctgtggt atggctgtgc aggtcgtaaa
     3301 tcactgcata attcgtgtcg ctcaaggcgc actcccgttc tggataatgt tttttgcgcc
     3361 gacatcataa cggttctggc aaatattctg aaatgagctg ttgacaatta atcatcggct
     3421 cgtataatgt gtggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa
     3481 ctttaagaag gagatatcat atgggatcct ctagagtcga caagcttggc tgttttggcg
     3541 gatgagagaa gattttcagc ctgatacaga ttaaatcaga acgcagaagc ggtctgataa
     3601 aacagaattt gcctggcggc agtagcgcgg tggtcccacc tgaccccatg ccgaactcag
     3661 aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga gtagggaact
     3721 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     3781 tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt
     3841 gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggcatcaa
     3901 attaagcaga aggccatcct gacggatggc ctttttgcgt ttctacaaac tcttttgttt
     3961 atttttctaa atacattcaa atatgtatcc gactagtgcc acggcctcta ggccagatcc
     4021 agcggcatct gggttagtcg agcgcgggcc gcttcccatg tctcaccagg gcgagcctgt
     4081 ttcgcgatct cagcatctga aatcttcccg gccttgcgct tcgctggggc cttacccacc
     4141 gccttggcgg gcttcttcgg tccaaaactg aacaacagat gtgtgacctt gcgcccggtc
     4201 tttcgctgcg cccactccac ctgtagcggg ctgtgctcgt tgatctgcgt cacggctgga
     4261 tcaagcactc gcaacttgaa gtccttgatc gagggatacc ggccttccag ttgaaaccac
     4321 tttcgcagct ggtcaatttc tatttcgcgc tggccgatgc tgtcccattg catgagcagc
     4381 tcgtaaagcc tgatcgcgtg ggtgctgtcc atcttggcca cgtcagccaa ggcgtatttg
     4441 gtgaactgtt tggtgagttc cgtcaggtac ggcagcatgt ctttggtgaa cctgagttct
     4501 acacggccct caccctcccg gtagatgatt gtttgcaccc agccggtaat catcacactc
     4561 ggtcttttcc ccttgccatt gggctcttgg gttaaccgga cttcccgccg tttcaggcgc
     4621 agggccgctt ctttgagctg gttgtaggaa gattcgatag ggacacccgc catcgtcgct
     4681 atgtcctccg ccgtcactga atacatcact tcatcggtga caggctcgct cctcttcacc
     4741 tggctaatac aggccagaac gatccgctgt tcctgaacac tgaggcgata cgcggcctcg
     4801 accagggcat tgcttttgta aaccattggg ggtgaggcca cgttcgacat tccttgtgta
     4861 taaggggaca ctgtatctgc gtcccacaat acaacaaatc cgtcccttta caacaacaaa
     4921 tccgtccctt cttaacaaca aatccgtccc ttaatggcaa caaatccgtc cctttttaaa
     4981 ctctacaggc cacggattac gtggcctgta gacgtcctaa aaggtttaaa agggaaaagg
     5041 aagaaaaggg tggaaacgca aaaaacgcac cactacgtgg ccccgttggg gccgcatttg
     5101 tgcccctgaa ggggcggggg aggcgtctgg gcaatccccg ttttaccagt cccctatcgc
     5161 cgcctgagag ggcgcaggaa gcgagtaatc agggtatcga ggcggattca cccttggcgt
     5221 ccaaccagcg gcaccagcgg cgcctgagag gcgaattgac ataagcctgt tcggttcgta
     5281 aactgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     5341 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttgtacagt
     5401 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
     5461 ggagcagcaa cgatgttacg cagcagcaac gatgttacgc agcagggcag tcgccctaaa
     5521 acaaagttag gtggctcaag tatgggcatc attcgcacat gtaggctcgg ccctgaccaa
     5581 gtcaaatcca tgcgggctgc tcttgatctt ttcggtcgtg agttcggaga cgtagccacc
     5641 tactcccaac atcagccgga ctccgattac ctcgggaact tgctccgtag taagacattc
     5701 atcgcgcttg ctgccttcga ccaagaagcg gttgttggcg ctctcgcggc ttacgttctg
     5761 cccaggtttg agcagccgcg tagtgagatc tatatctatg atctcgcagt ctccggcgag
     5821 caccggaggc agggcattgc caccgcgctc atcaatctcc tcaagcatga ggccaacgcg
     5881 cttggtgctt atgtgatcta cgtgcaagca gattacggtg acgatcccgc agtggctctc
     5941 tatacaaagt tgggcatacg ggaagaagtg atgcactttg atatcgaccc aagtaccgcc
     6001 acctaacaat tcgttcaagc cgagatcggc ttcccggccg cggagttgtt cggtaaattg
     6061 tcacaacgcc gcggccaatt cgtatggtgc actctcagta caatctgctc tgatgccgca
     6121 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     6181 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     6241 ttttcaccgt catcaccgaa acgcgcga
//
