LOCUS       40924_41954        5961 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Mammalian expression vector pSVhR55d327-426, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5961)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5961)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5961)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5961
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(750..884)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1309..1329)
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(1459..1524)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(1526..1600)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL87871.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     misc_feature    complement(1638..2060)
                     /label=3UTR
                     /note="3UTR"
     misc_feature    complement(2188..2226)
                     /label=E-tag
                     /note="E-tag"
     CDS             complement(2236..3210)
                     /codon_start=1
                     /note="unnamed protein product; TNFRd"
                     /protein_id="SJL87872.1"
                     /translation="MVPHLGDREKRDSVCPQGKYIHPQNNSICCTKCHKGTYLYNDCPG
                     PGQDTDCRECESGSFTASENHLRHCLSCSKCRKEMGQVEISSCTVDRDTVCGCRKNQYR
                     HYWSENLFQCFNCSLCLNGTVHLSCQEKQNTVCTCHAGFFLRENECVSCSNCKKSLECT
                     KLCLPQIENVKGTEDSGTTVLLPLVIFFGLCLLSLLFIGLMYRYQRWKSKLYSIVCGKS
                     TPEKEGELEGTTTKPLAPNPSFSPTPGFTPTLGFSPVPSSTFTSSSTYTPGDCPNFAAP
                     RREVAPPYQGADPILATALASDPIPNPLQKWEDSAHKPQSLDTD"
     misc_feature    complement(3211..3297)
                     /label=sTNFR
                     /note="sTNFR"
     misc_feature    complement(3298..3378)
                     /label=5UTR
                     /note="5UTR"
     promoter        complement(3390..3719)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(4134..4722)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4896..5753)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5754..5858)
                     /label=AmpR promoter
ORIGIN
        1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg
       61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac
      121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg
      181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc
      241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc
      301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa
      361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg
      421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct
      481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca
      541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc
      601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga
      661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg
      721 tttatgagga cacagaggag cttcctgggg atccagacat gataagatac attgatgagt
      781 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
      841 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
      901 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
      961 tctacaaatg tggtatggct gattatgatc tctagtcaag gcactataca tcaaatattc
     1021 cttattaacc cctttacaaa ttaaaaagct aaaggtacac aatttttgag catagttatt
     1081 aatagcagac actctatgcc tgtgtggagt aagaaaaaac agtatgttat gattataact
     1141 gttatgccta cttataaagg ttacagaata tttttccata attttcttgt atagcagtgc
     1201 agctttttcc tttgtggtgt aaatagcaaa gcaagcaaga gttctattac taaacacagc
     1261 atgactcaaa aaacttagca attctgaagg aaagtccttg gggtcttcta cctttctctt
     1321 cttttttgga ggagtagaat gttgagagtc agcagtagcc tcatcatcac tagatggcat
     1381 ttcttctgag caaaacaggt tttcctcatt aaaggcattc caccactgct cccattcatc
     1441 agttccatag gttggaatct aaaatacaca aacaattaga atcagtagtt taacacatta
     1501 tacacttaaa aattttatat ttaccttaga gctttaaatc tctgtaggta gtttgtccaa
     1561 ttatgtcaca ccacagaagt aaggttcctt cacaaagatc cgtcgactct agcggtaccg
     1621 agctcgaatt cgggcgggtg tatgtacaaa agtccacagc tccagctgaa ggccccattg
     1681 ttccgtgctc gcccctgcct taggacagtt cagcttgcta tgtgcttgtc caggcagagg
     1741 gcacaggagt gccaagtttc tattagtgta acatgattga tttaaaaaca aaacaaaaca
     1801 aaacaaaaac aaaaaaaact gcttatgcac tgtgaaaaag gctcagggac gaaccagggg
     1861 cccccgagca gccttgctgg tgaggacacc caaaacgggc atgaggcata gcgtccctca
     1921 tcctcgcaaa ccacccactc aggctcttga gcccacggcg cacctctctc cgcgcgcaca
     1981 gcgctgactg tcggcggcgc gcaggcagct gagaaaagct atgtacatcg aggggttagc
     2041 accaagtagg cggctgctag agtcgacatt ccacagacag ccctcatagt tagcgtaacg
     2101 atctaaagtt ttgtcgtctt tccagacgtt agtaaatgaa ttttctgtat gaggttttgc
     2161 taaacaactt tcaacagtct atgcggcacg cggttccagc ggatccggat acggcaccgg
     2221 cgcacctgcg gccgcatcag tgtctaggct ctgtggcttg tgggcgctgt cctcccactt
     2281 ctgaaggggg ttggggatgg ggtcggaggc gagggctgtc gcaaggatgg ggtcagcccc
     2341 ctgatagggt ggtgccacct ctctgcgggg agccgcaaag ttgggacagt caccgggggt
     2401 ataggtggag ctggaggtga aggtggaact gggcacggga ctgaagccca gggtgggggt
     2461 gaagcctgga gtgggactga agcttgggtt tggggccagg ggcttagtag tagttccttc
     2521 aagctccccc tctttttcag gtgtcgattt cccacaaaca atggagtaga gcttggactt
     2581 ccaccgttgg tagcgataca ttaaaccaat gaagaggagg gataaaaggc aaagaccaaa
     2641 gaaaatgacc aggggcaaca gcactgtggt gcctgagtcc tcagtgccct taacattctc
     2701 aatctggggt aggcacaact tcgtgcactc caggcttttc ttacagttac tacaggagac
     2761 acactcgttt tctcttagaa agaaacctgc atggcaggtg cacacggtgt tctgtttctc
     2821 ctggcaggag aggtgcacgg tcccattgag gcagaggctg caattgaagc actggaaaag
     2881 gttttcactc caataatgcc ggtactggtt cttcctgcag ccacacacgg tgtcccggtc
     2941 cactgtgcaa gaagagatct ccacctgacc catttccttt cggcatttgg agcagctgag
     3001 gcagtgtctg aggtggtttt ctgaagcggt gaaggagccg ctctcacact ccctgcagtc
     3061 cgtatcctgc cccgggcctg gacagtcatt gtacaagtag gttcctttgt ggcacttggt
     3121 acagcaaatc gaattatttt gagggtggat atattttcct tggggacaca cactatctct
     3181 cttctccctg tcccctaggt gagggaccag tccaataacc cctgaggggt atattcccac
     3241 caacagctcc aggagcacca gcggcagcag caggtcaggc acggtggaga ggcccatgcc
     3301 agacagctat ggcctctcac tcccccattt gggctcaggg cagtgtggca gcggcagtgc
     3361 tggggcttcc cgggactcag cttgagcttt ttgcaaaagc ctaggcctcc aaaaaagcct
     3421 cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa
     3481 aaaattagtc agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg
     3541 ggcggagtta ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt
     3601 ctgcctgctg gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg
     3661 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca
     3721 ttccacagtc gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt
     3781 tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac
     3841 gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa
     3901 tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ctcttccgct
     3961 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     4021 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     4081 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     4141 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     4201 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     4261 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     4321 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     4381 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     4441 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     4501 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     4561 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     4621 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     4681 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     4741 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     4801 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     4861 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     4921 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     4981 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     5041 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     5101 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     5161 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg
     5221 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     5281 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     5341 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     5401 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     5461 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat
     5521 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     5581 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     5641 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     5701 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     5761 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     5821 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     5881 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     5941 aggccctttc gtcttcaaga a
//
